HumanKine Tumor Necrosis Factor , human

recombinant, expressed in HEK 293 cells

Catalog Number H8916
Storage Temperature 20 C
Synonyms: TNF-, cachectin
Product Description
HumanKine TNF- is expressed in human 293 cells
and has been shown to be predominantly a
glycosylated, non-covalently linked homotrimer with a
molecular mass of 51 kDa (gel filtration). Production in
human 293 cells offers authentic glycosylation.
Glycosylation contributes to stability in cell growth
media and other applications.
Tumor Necrosis Factor- (TNF-) is a protein secreted
by lipopolysaccharide-stimulated macrophages and
causes tumor necrosis in vivo when injected into
tumorbearing mice.1 TNF- is believed to mediate
pathogenic shock and tissue injury associated with
endotoxemia.2
TNF- is closely related to the 25 kDa protein Tumor
Necrosis Factor- (lymphotoxin), sharing the same
receptors and cellular actions.3 TNF- causes cytolysis
or cytostasis of certain transformed cells,4,5 being
synergistic with -interferon in its cytotoxicity.6 Although
it has little effect on many cultured normal human cells,5
TNF- appears to be directly toxic to vascular
endothelial cells.7
This product is lyophilized from a PBS solution.
ED50: 1.00 ng/mL
The specific activity was determined by the dosedependent cytotoxity of the TNF- sensitive cell line
L-929 in the presence of actinomycin D.
Purity: 95%
Endotoxin level: 1 EU/g

Precautions and Disclaimer

This product is for R&D use only, not for drug,
household, or other uses. Please consult the Material
Safety Data Sheet for information regarding hazards
and safe handling practices.
Preparation Instructions
Briefly centrifuge the vial before opening. It is
recommended to reconstitute the protein in sterile PBS
containing 0.1% endotoxin-free recombinant human
serum albumin.
Storage/Stability
Store the product at 20 C. The lyophilized product
remains active for one year at 20 C.
Upon reconstitution, the cytokine can be stored at
28 C for short term only, or at 20 C to 80 C in
aliquots for long term. Avoid repeated freeze-thaw
cycles.
References
1. Carswell, E.A., et al., Proc. Natl. Sci. USA, 72,
3666 (1975).
2. Tracey, K.J., et al., Science, 234, 470 (1986).
3. Hass, P.E., et al., J. Biol. Chem., 260, 12213
(1985).
4. Granger, G.A., et al., J. Clin. Immunol., 5, 217
(1985).
5. Helson, L., et al., Nature, 258, 731 (1975).
6. Williamson, B D., et al., Proc. Natl. Acad. Sci. USA,
80, 5397 (1983).
7. Sato, N., et al., J. Natl. Cancer Inst., 76, 1113
(1986).
HumanKine is a trademark of HumanZyme Inc.
GS,JF,MAM 12/10-1

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