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Topic 2-Questionanswer
Topic 2-Questionanswer
Objective
Magnification
4x
10x
40x
Ocular
Magnification
10x
10x
10x
Total
Magnification
40x
100x
400x
Downward/toward you
7. Suppose you were looking at a small, motile organism in a drop of water under the microscope.
The organism was moving across your field of view from left to right as you observed it under
the microscope, and was about to move out of the field. In which direction would you need to
move the slide to bring the organism back into the center of the field?
Move right. When the slide is moved right, things in the field of view move to the left.
Questions: B. Field of View
TABLE 2-2. Diameter of Field of View under Various Magnifications
Objective Lens
Scanning (measured)
Low (measured)
High (calculated)
Diameter in mm
Answer will vary
Answer will vary
Answer will vary
Diameter in m
Answer will vary
Answer will vary
Answer will vary
Scanning
3. What is the relationship between magnification of the objective and depth of focus?
Higher magnification decreases the depth of focus.
Questions: B. Provision of ContrastBacteria of the Human Mouth
1. What do you observe? Sketch the cells.
(Sketched drawing)
2. Are these cells more easily visible than those of the same type found in the hay infusion?
May differ for each student depending on how long the hay infusion ahs been in H2O
and how well the smear was done.
Questions: III. The Stereoscopic Dissecting Microscope
1. What is the advantage of one type of light over the other?
Advantage of transmitted light: able to see the entire slide of a whole mount that is too
large to view on a compound microscope.
Advantage of reflected light: able to see large specimens and see detail of small
specimens.
2. How do you adjust the brightness of the light?