Mia Dilone

Stem -10
Catalase control and experiment
Enzymes are proteins that increase the rate of reaction. Substrate is
like the receptors or hormones that connect to enzymes. What we did
was to try to change the speed of the magnetic bar. How we try to
change it was to experiment different amounts of speed we changed
them to see at which best would increase or change how fast it goes. We
used hydrogen peroxide which basically digested into water and oxygen
and we also used a type of enzyme called catalase. The enzymes reacts
depends on how much catalase you put and depends on what
temperature or speed amount you have it on.
When we tried out our experiment or when we predicted our
hypothesis, it came out to be wrong. When we changed the speed and
the stirring metal bar did not react. It did not go faster than we expected
it to go. When we did this trial the first graph shows the slope of 0.3787
kpa/min. Now the second time we tried it out there was a drastic change
between the both of them. The second graph shows a slope of 30.90
kpa/min. The second time the metal bar barely reacted. We knew that
the enzyme will react but when we changed the speed it wasnt
shocking or intriguing.
Overall this procedure was minor easy because we were able to
create our own prediction/ hypothesis, we changed the materials put in
the flask in order to come up with a way to make the metal bar react.
This experiment did not work as well as we thought it was going to
work. The second time it did not change in speed of nothing. We tried to

find another way in order to increase the rate but it would all be a
different process.

Catalase Lab Procedure

1. _____all_____Check that you have all the equipment below.
Computer with Internet access and Vernier LoggerPro software
LabQuest Mini
Vernier Gas Pressure Sensor
Two-hole black rubber stopper (top)
Plastic tubing with Luer-lock connectors
20-200 L micropippetor set to 100 L
micropipette tips
50mL graduated cylinder
125 mL or 250 mL Erlenmeyer flask
Magnetic stirrer
Stirring bar
Thermometer
Metal clamp stand
At the central table:
Hydrogen peroxide
Catalase enzyme suspension
2. ____all______Check that every person is wearing apron and goggles.

3. ____all______Check that everyone who will touch the enzyme or liquids is

wearing thick latex gloves (blue and yellow gloves)
On the blank lines, write the initials of the teammate who completed the task:
4. __________Open your laptop and open Logger Pro .
5. __________Click File Open and open the folder in Biology by
Vernier. Then choose 06 Enzyme (Pressure).
6. __________Connect the LabQuest Mini to the computer using the USB
cable, and connect the Gas Pressure Sensor to CH 1 of the LabQuest Mini.
Set up the laboratory apparatus as seen in the picture:
7. __________Measure out 50mL of 1.5% H2O2
and pour into an Erlenmeyer flask.
8. __________Carefully place a magnetic stir bar in
the flask.
9. __________Place the flask on a magnetic stir
plate. Use a clamp to fasten the flask to the ring
stand as shown. Position the flask at the center
of the magnetic stirrer.
10. __________Test the stirrer at 100 rpm.
11. __________Stop the stirrer.
12. __________Use the plastic tubing with two Luerlock connectors to connect the two-hole rubber
stopper assembly to the Gas Pressure Sensor as shown in the image. The
valve connected to the stopper should stay closed during this investigation.

Its closed when its flat, parallel to the floor

Complete all the steps below quickly to complete your test reaction.
13. __________Start data collection: click green Collect button on Logger Pro.
14. __________Using a micropipette, add 100 L of enzyme suspension to the
contents of the flask.
15. __________IMMEDIATELY tightly seal the flask by placing the stopper in and
HOLDING it in carefully.
16. __________IMMEDIATELY Turn the stir plate on to 100.
NOTE: If the pressure exceeds 130 kPa, the pressure inside the flask will be
too great and the rubber stopper will likely pop off. HOLD DOWN the
stopper or be ready to have uncovered chemicals on your desk
WAIT 200 seconds (3.3 minutes) while data is
collecting- Do NOT click STOP. Data collection will automatically stop
after 200 seconds.
17. __________Turn off the stir plate.
18. __________Carefully remove the stopper from the flask to relieve the
pressure.
19. __________Use a thermometer to test and record the temperature of the
liquid in your lab packet
20. __________Pour all chemical waste into a RED bucket.
21. __________Remove the magnetic bar.
22. __________ Dispose of your used micropipette tip.

23. ___all_______Take off your safety gear and place it neatly away.
24. Observe the graph generated using LoggerPro software (example shown
below).
25. Hold down Control on the keyboard and press j to zoom in the graph.
26. Highlight the section of the graph where the slope is increasing, by clicking
and dragging your mouse across it.
27. Click the Analyze tab at the top of the page, and choose Linear Fit. A
statistics box will appear for your highlighted section of the graph.
28. Record the slope of the line, m, as the rate of catalase activity in kPa/s in
your lab packet (page 13)
29. Click File, Save As, and save with the file name:
(Your Names) Sam, Anabel, Jenni, Jose Control Trial Logger Pro Data
Email the graph to Ms. Lenowitz by following these final steps!
30. Click File and choose Print Graph. In the drop down menu of printers, choose
Cute PDF Writer in the drop down list. This will export your graph as a
PDF file. Be patient- it takes a few seconds to pop up.
31. Save the PDF with the this file name:
(Your Names) Sam, Anabel, Jenni, Jose Control Trial Data PDF
32. Open your MESA email and Compose a new email to
glenowitz@mesacharter.org
33. Attach the PDF to the email.
34. Press send!