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INTRODUCTION
bacterial strains).
- Several active compounds have less inhibitory effect on Pseudomonas spp. than
on Bacillus spp., due to the presence of lipoproteins and liposaccharides on the outer
layer of peptidoglycane.
- In Pseudomonas spp., the higher content of phospholipids and n e u t r a l lipids
increases resistance. Benzalkonium chloride makes the cell more permeable. The same
phenomenon is observed in Enterobacter cloacae.
- In Gram-positive bacteria, the product becomes bound to the wall proteins and is
thus able to enter and destroy the membrane.
- U n i f o r m a b s o r p t i o n may be observed in Gram-positive and G r a m - n e g a t i v e
bacteria, c o r r e s p o n d i n g to an increase in permeability and loss of viability
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Iodine also interacts preferentially with the proteins of the cytoplasm membrane in a
form with a positive ( H 0 +1) or neutral (I or H O I ) charge.
2 2
In the presence of oxidising agents such as peroxides, bacteria such as E. coli defend
themselves by producing enzymes which either destroy the oxidising agent before
bacterial degradation takes place or help the restoring mechanisms.
Oxidised molecules are more sensitive to proteolysis than other molecules, and it has
been suggested that a 'system of cell sanitisation' may enter into effect under the action
of an oxidising agent such as H 0 . 2 2
Ozone (21)
O z o n e p r o b a b l y acts on bacteria by oxidation. In viruses, ozone inactivates
bacteriophages F2 and T4 by attacking the protein capsid to release and then inactivate
the nucleic acids.
Aldehyde compounds (6, 21)
Formaldehyde
Formaldehyde acts on proteins by denaturation and on nucleic acids by alkylation, as
follows:
0 = CH o 2 Protein-NH-CH -OH 2
The primary site of action is the cytoplasmic membrane, with resulting modification
of m e m b r a n e permeability. This effect was observed to be due to electrostatic
interaction of the P H M B with the acid phospholipids in the cytoplasmic m e m b r a n e .
Given the size of the P H M B molecules, this implies actual absorption o n t o the
cytoplasmic m e m b r a n e of G r a m - p o s i t i v e or G r a m - n e g a t i v e bacteria, leading to a
destructive effect at this level. Unlike certain antibiotics (penicillins, bacitracin and
novobiocin), there is no accumulation of bacterial wall precursors; destruction is caused
by rupture of the membrane and loss of permeability without lysis of the cell wall.
R e l e a s e of the cell constituents occurs at very low concentrations. At the high
concentrations used under antiseptic conditions, the bactericidal effect is very rapid,
due to coagulation of the cytoplasm. Therefore, bacterial death is not due to leakage of
the cell constituents at all concentrations of P H M B .
CONCLUSION
Although the notion of a target in the bacterial cell is frequently evoked in the case of
antibiotics, this aspect remains r a t h e r vague with regard to disinfectants. N o r can
disinfectant action be considered specific to a particular bacterial species, whereas such
specificity is commonplace in antibiotics. A t most, disinfectants may be said to have
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REFERENCES
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disinfectants. Chem. Mikrobiol. Technol. Lebensm., 9, 11-13.
18. N O L L H . & YOUNGNER J . S . (1959). - Virus-lipid interactions. The mechanism of
adsorption of lipophilic viruses to water insoluble polar lipids. Virology, 8, 319-343.
19. OSANAI S. & A B E Y . (1985). - Chirality, antimicrobial relationship of quaternary
ammonium cationies. J. chem. tech. Biotechnol., 35B, 43-45.
20. RICHARDS R . M . E . & CAVILL R . H . (1976). - Electron microscope study of effect of
benzalkonium and edetate de sodium on cell envelope of Pseudomonas aeruginosa.
J. pharm. Sci., 65,76-79.
21. RUSSELL A.D. (1983). - Principles of antimicrobial activity. In Disinfection, sterilization
and preservation, 3rd Ed. ( S . S . Block, ed.). Lea & Febiger, Philadelphia, 717-745.
22. RYTER A. & DODIN A. (1988). - Altrations structurales provoques par des antiseptiques
chez diverses espces bactriennes. Bull. Soc. pathol. exot., 81,811-818.
23. SAKAGAMI Y , YOKOYAMA H . , NISHIMURA H . , O S E Y. & TASHIMA T. (1989). - Mechanism
of resistance to benzalkonium chloride by Pseudomonas aeruginosa. Appl. environ.
Microbiol, 55,2036-2040.
24. SCOTT F.W. (1979). - Virucidal disinfectants and feline viruses. Am. J. vet. Res., 41,410-414.