Methods

1. Acclimate flies to environment. There should be 4 groups of flies(1

genetically modified w/ low concentration of celecoxib, 1 genetically
modified w/ high concentration of celecoxib, 1 genetically modified
w/o drug, 1 with no genetic modification). Give them a constant food
source in a constant environment.
2. Place celecoxib in food source of two groups of the flies. Place .15
grams of celecoxib in the group with a low concentration and .30
grams of celecoxib in group with high concentration.
3. Train flies to recognize odor 1 versus odor 2. When the flies are
exposed to odor 1, place them in a new container with 3 grams of
sucrose for 7 minutes. Repeat over next three days.
4. Build T-Maze
5. Place fruit flies in maze and observe to which odor they fly to. Give
sugar to flies that go to the left, which is odor 1. Repeat for 4 days, and
continue to monitor which side they go to.
6. Once experimentation is complete, euthanize fruit flies by placing in
the freezer for 20 minutes.

Materials
Drosophila melanogaster 2 liquid odors(placed on
1 strain without genetic foam tops)
modification Sucrose
3 strains with T-maze
modification in APP Freezer
2 containers of Drosophila Mortar and pestle
dry media source(food) Balance
15 containers for fruit flies Gloves
with foam tops Pipettes
1 200 mg container of Water
celecoxib

Conclusion
My hypothesis was supported by these results. These results
showed that fruit flies with an APP mutation had a more
sporadic pattern when choosing the odor, while flies that had
this mutation but were given celecoxib tended to choose the
odor that related to sucrose, which was more similar to the
control(the group with no mutation). This demonstrates that
the celecoxib caused the fruit flies to regain memory function,
most likely by targeting the amyloid plaques in the NLRP3
inflammasome. This data was also proven statistically
significant, showing that there is a real correlation between
celecoxib and memory functioning.

Impact
While these findings did show that celecoxib did reduce AD
symptoms, there is still quite a bit of experimentation that
should be done on how NSAIDs affect Alzheimers disease.
This project was on such a small scale, and while there are
many similarities to genetics and memory functioning of fruit
flies and humans, there is still a long way to go before this will
be carried into an effective treatment for humans. Because
Alzheimers is such a devastating disease and has become so
prevalent in our world, it is important to continue to look at
ways to treat it, and it is also important to look at existing
medication and treatments to see how they can be used to find
a cure for this disease.

Abstract, Safety, and Works

Cited