SEG-A-092

WIDE PH VERSION
NOW AVAILABLE!

Extent your pH range from pH 1 to 14 with the Nexera wide pH kit*

Any standard Nexera HPLC/UHPLC system can be converted into a wide pH version by changing the following parts:

Part number Name

228-57729-41 Wide pH kit - SIL
228-57725-41 Wide pH kit - LC-30AD

Optional: Special MR mixers

Part number Name Remark

228-45209-41 MR mixer 20 For binary gradient, 20 L
228-45209-42 MR mixer 40 For binary gradient, 40 L
228-45209-43 MR mixer 100 For binary gradient, 100 L
228-45209-44 MR mixer 180 II For binary gradient, 180 L

Change in specification of Nexera wide pH version

SIL-30AC wide pH SIL-30AC standard

Available pH range 1 to 14 1 to 9
Maximum pressure 130 MPa (19,000 psi) 130 MPa (19,000 psi)
Material for needle seal PEEK Polyimide
Replacement Every 40,000 injections Every 40,000 injections

LC-30AD, MR mixer wide pH LC-30AD, MR mixer standard

Available pH range 1 to 14
Maximum pressure 130 MPa (19,000 psi)
PEEK, SUS316L, ruby, sapphire,
Wetted part materials
HastelloyC, polyethylene
1 to 9
130 MPa (19,000 psi)
Polyimide, PEEK, SUS316L, ruby,
sapphire, HastelloyC, polyethylene

* Please contact us to check the availability of these products in your country.

 pH kit 1.0/E/04/14/L

Nexera X2 wide pH version

The Nexera X2 system was developed to handle ultra-fast, ultra-high resolution applications at system backpressure up to
130 MPa. As all-round LC system it supports conventional as well as ultra-high pressure applications without changes on the
flow line, and with the high pH conversion kit it now also allows to work in a pH range of 1- 14.

Changing the pH of a mobile phase is a powerful tool to obtain significant changes in the selectivity of a reversed-phase
HPLC separation. Ionizable compounds, meaning acidic, basic or zwitterionic analytes will show extreme changes in retention
depending on their pKa and pH of the mobile phase. Therefore, a wide pH range of the HPLC equipment offers additional
possibilities for HPLC method optimization by exploiting selectivity changes at low, intermediate and high pH.

Example: Separation of seven pharmaceuticals in a) acidic or b) basic mobile phase conditions

5/3.728

a)
3/2.080
1/1.816
2/1.963

4/3.259

6/4.631

7/5.877
3/3.435
2/1.889

b)
1/2.895

4/3.277
5/1.761

6/6.571
7/5.909

0.0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 5.0 5.5 6.0 6.5 min

1. Nizatidine 2. Amiloride 3. N-Acetylprocainamide 4. Quinoxaline 5. Methyl paraben 6. Reserpine 7. Toluene

Shimadzu Europa GmbH

Albert-Hahn-Str. 6-10 D-47269 Duisburg
Tel.: +49 - (0)203 - 76 87-0
Fax: +49 - (0)203 - 76 66 25
shimadzu@shimadzu.eu
www.shimadzu.eu

To find your local Shimadzu contact

please visit www.shimadzu.eu