Lampiran 2.

Perbandingan Protein-Protein Homolog

Penentuan Persen Homologi

1. RefSeq, RefSeqgene (Homo sapiens)

MRAGRGATPARELFRDAAFPAADSSLFCDLSTPLAQFREDITWR
RPQEICATPRLFPDDPREGQVKQGLLGDCWFLCACAALQKSRHLLDQVIPPGQPSWAD
QEYRGSFTCRIWQFGRWVEVTTDDRLPCLAGRLCFSRCQREDVFWLPLLEKVYAKVHG
SYEHLWAGQVADALVDLTGGLAERWNLKGVAGSGGQQDRPGRWEHRTCRQLLHLKDQC
LISCCVLSPR AGARELGEFH AFIVSDLREL QGQAGQCILL LRIQNPWGRR CWQGLWRE
GGEGWSQVDA AVASELLSQL QEGEFWVEEE EFLREFDELT VGYPVTEAGH LQSLYTER
LLCHTRALPG AWVKGQSAGG CRNNSGFPSN PKFWLRVSEP SEVYIAVLQR SRLHAADW
AGRARALVGD SHTSWSPASI PGKHYQAVGL HLWKVEKRRV NLPRVLSMPP VAGTACHA
YDREVHLRCE LSPGYYLAVP STFLKDAPGE FLLRVFSTGR VSLSAIRAVA KNTTPGAA
LPAGEWGTVQLRGSWRVGQ TAGGSRNFAS YPTNPCFPFS VPEGPGPRCV RITLHQHCRP 50
SDTEFHPIGF HIFQVPEGGR SQDAPPLLLQ EPLLSCVPHR YAQEVSRLCL LPAGTYKVVP
STYLPDTEGA FTVTIATRID RPSIHSQEML GQFLQEVSVM AVMKT
2. RefSeq (Gorilla gorilla gorilla)

MRAGRGATPARELFRDAAFPAADSSLFCDLSTPLAQFREDITWR
RPQEICATPRLFPDDPREGQVKQGLLGDCWFLCACAALQKSRHLLDQVIPPGQPSWAD
QEYRGSFTCRIWQFGRWVEVTTDDRLPCLAGRLCFSRCQREDVFWLPLLEKVYAKVHG
SYEHLWAGQVADALVDLTGGLAERWNLKSVAGSGGQQDRPGHWEHRTCRQLLHLKDQC
LISCCVLSPRAGARELGEFHAFIVSDLRELQGQAGQCILLLRIQNPWGRRCWQGLWRE
GGEGWSQVDAVVASELLSQLQEGEFWVEEEEFLREFDELTVGYPVTEAGHLQSLYTEK
LLCHTRALPGAWIKGQSAGGCRNNSGFPSNPKFWLRVSEPSEVYIAVLQRSRPHAADW
AGRAQALVGDSHTSWSPASIPGKHYQAVGLHLWKVPEG GRSQDAPPLL VQEPLLSCVP
H RYAQEVSRL C LLPAGTYKV V PSTYLPDTE GA FTVTIATR IDRP SIHSQE MLGQF LQE
VSVMAVM KT

Diketahui :

Total AA sekuen pertama (La) = 672 AA

Total AA sekuen kedua (Lb) = 517 AA

Total AA yg beda = 92 AA

Jumlah AA yang mirip (Ls) = 425 AA

Ditanya :

Persen homologi = ....?

Jawab :
2
S = + 100

4252
S = 672+517 100 = 71,48%

Jadi, persen homologi kedua sekuen tersebut adalah sebesar 71,48%