KENDRIYA VIDYALAYA SANGATHAN, BHUBANESWAR REGION

SAMPLE QUESTION PAPER- 4

Sub: BIOLOGY Class: XII
Time allowed: 3hrs Maximum Marks: 70

BLUE PRINT

MARKS VSA SA II SA I VBQ LA TOTAL

UNIT 1M 2M 3M 4M 5M

I (Reproduction) 1(1m) 1(2m) 2(6m) - 1(5m) 14 m

II ( Genetics) 1(1m) - 4(12m) - 1(5m) 18 m

III (Biology in 1(1m) 2(4m) 3(9m) - - 14m

human welfare)
IV (Biotechnology) 2(2m) - 1(3m) - 1(5m) 10m

V Ecology and - 2(4m) 2(6m) 1(4m) - 14m

Environment
Total 5(5m) 5(10m) 12(36m) 1(4m) 3(15m) 70 m
 KENDRIYA VIDYALAYA SANGATHAN, BHUBANESWAR REGION
SAMPLE QUESTION PAPER- 4
Sub: BIOLOGY Class: XII
Time allowed: 3hrs Maximum Marks: 70
General Instructions:
1. There are a total of 26 questions and five sections in the question paper. All questions are
compulsory
2. Section A contains question number 1 to 5, Very Short Answer type questions of one mark
each.
3. Section B contains question number 6 to 10, Short Answer type I questions of two marks
each.
4. Section C contains question number 11 to 22, Short Answer type II questions of three
marks each.
5. Section D contains question number 23, Value Based Question of four marks.
6. Section E contains question number 24 to 26, Long Answer type questions of five marks
each.
7. There is no overall choice in the question paper, however, an internal choice is provided in one
question of two marks, one question of three marks and all three questions of five marks. An
examinee is to attempt any one of the questions out of the two given in the question paper with
the same question number.
SECTION-A
1. Identify the vegetative propagule in the following –

2. A child suffering from Down’s syndrome shows trisomy of 21st

chromosome. Mention the cause of this abnormality?
3. State the importance of biofortification.
4. How is the action of exonuclease different from that of an endonuclease?
5. Name the easier technique to differentiate recombinants from the non-recombinants
other than inactivating antibiotic resistance gene.
SECTION-B
6. ‘Saheli’ is widely accepted as ideal methods of contraception by women. Give any 4
reasons to highlight its advantage over other methods.
7. A person shows a strong immunogenic reaction while exposed to certain
substances. Name this condition and the common term for such substances.
Mention the cell and the chemical which causes this condition.
8. How are somatic hybrids produced?
9. How do mycorrhizae act as biofertilizer? Explain. Name the genus of the fungi
that forms mycorrhizal associations with plants.
OR
Name the source of Streptokinase. What is the role of this bioactive molecule in the
human body.
10. Name the type of interaction in each of the following-
a) Sucker fish attached to shark b) wasp pollinating fig inflorescence
c) Smaller barnacles disappeared when Balanus dominated the coast of Scotland.
d) Ascaris lives in the intestine of man.
 SECTION-C
11. Mention any four strategies adopted by flowering plants to prevent self pollination.Why is
bagging done by plant breeders? How many times will it be done during a cross?
12. What is gestation period?Give the technical word for child birth.What signals the process
of child-birth?? What does it result in initially? Name the hormone that is released by this
event.Name the kind of milk that is produced in the initial stages of lactation.
13. Name and explain the evolutionary concept represented in the illustration given
below. What type of evolution does it show – Convergent / divergent?

14. During the process of transcription in Eukaryotes, the primary transcript formed
is very different from the mature RNA.
a) What is the primary transcript called as?
b) How is it different from the mature RNA ?
c) Explain the steps in the formation of mature RNA from the Primary transcript.
15. The amino acid composition of a portion of the chain of haemoglobin is shown
below:

The codon for the sixth amino acid is GAG.The sixth codon GAG mutates to GAA
as a result of mutation’A’ and to GUG as as a result of mutation ‘B”. Haemoglobin
structure did not change as a result of mutation’A’ whereas it became sickle shaped
as a result of mutation ‘B’ .Explain giving reasons how is this possible
16. (a) Describe the series of experiments of F.Griffith. Comment on the significance of
the results obtained. (b) State the contribution of Macleod, Mc Carty and Avery.
17. MOET is considered to be a successful programme in cattle breeding. Explain the
steps involved in carrying out this programme in cows.
18. Differentiate between a benign and a malignant tumor.(any 2) How do cancerous
cells differ from normal cells? b) What is the role of interferons in the treatment of
cancer ?
19. Fill in the blank spaces a, b, c, d, e, and f, given in the following table :
Name of organism Commercial product Application
Penicillium notatum Penicillium a
b Lactic acid Making Curd
Streptococcus Clot buster enzyme c
Trichoderma polysporum d Immuno suppressive agent
Saccharomyces cerevisiae ethanol e
f Swiss cheese Food Product

20. Observe the given sequence of nitrogenous bases on a DNA fragment and answer the
following questions.
5’ – GAATTC – 3’
3’ – CTTAAG - 5’
(a) Name of restriction enzyme which can recognise this DNA sequence. (b) Write the
sequence after digestion. (c) Why are the ends generated after digestion called sticky
ends?
21. A population 100 spotted deer were living without any carnivores in an enclosure of a
few hectares of tropical forest land. The deer census was taken after a few years.
Now study the graph given below and answer the questions that follow-

a) Identify the curve that represents the deer population.

b) What is this this type of population growth curve called as?
c. What is ‘K’ in the graph?
22. a. Draw a pyramid of numbers of a situation ,where a large population of insects
feed upon a very big tree. The insects in turn are eaten by small birds, which
in turn are fed upon by big birds.
b) Differentiate giving reasons between the pyramid of energy of the above and the
pyramid of number that you have drawn.
23. Manas was waiting at a bus stop. Many passengers along with their kids were on
Their way to school. A bus came and painted the children with black smoke ejected
from the exhaust pipe. Manas immediately stopped the bus and called the
conductor and driver to show what they had done. Passengers waiting in the bus
stop supported Manas while those on board became restless for being delayed.
a. What values did Manas promote through his action?
b. Why are children more affected by vehicle exhausts? 1
c. Cars are seen with Bharat stage IV stickers. What does it imply? 1
d. How do catalytic converters reduce vehicular gas emission?
24. a)Show diagrammatically the stages of embryonic development from zygote to
implantation in humans.
b) Briefly explain the formation of chorionic villi and the placenta in embryonic
development.
 OR
a) Diagramatically show the stages in the development of megaspore mother cell to from
a mature embryo sac in angiosperms.
b) Why is this type of embryo sac development called as monosporic?

25. a)A true breeding homozygous pea plant with green pods and axial flowers as
dominant characters is crossed with a homozygous recessive pea plant with
yellow pods and terminal flowers. Work out the cross upto F2 generation. Give
the phenotypic ratio of the F2 .
b) Name the law that can be deduced from this cross and not from a monohybrid
cross.
OR
How did Alfred Hershey and Martha Chase prove that DNA is the hereditary material?
Explain.
26. What are the three different approaches of treating ADA deficiency? Which one is permanent
cure for it? How is such treatment carried out. Explain the process in a sequential order.
OR
What is meant by “making the host cell competent? Explain different methods
employes in making plant, animal and bacterial cells competent.
 MARKING SCHEME OF SAMPLE QUESTION PAPER- 4
CLASS XII BIOLOGY
Q. Suggestive answer Marks Total
No. distribu
tion
1 offset 1 1
2 Failure of segregation of chromatids during cell division/ Non- 1 1
disjunction of chromosomes during meiosis
3 Helps in improving the nutritional quality of crops . 1 1
4 Exonuclease removes nucleotides from the ends of the DNA endonuclease ½ +½ 1
cuts the DNA at specific sites
5 Insertional inactivation of a-galactosidase enzyme gene. 1 1
6 Once a week pill, very few side effects, non-steroidal,high contraceptive value ½X4 2
7 Allergy(1/2), allergens(1/2), Mast cells(1/2) histamine and serotonin( any one)(1/2) ½X4 2
8. Isolation of plant cells(1/2), digesting their cell walls(1/2) - isolation of the naked ½X4
protoplasts(1/2)- Fusion of isolated protoplasts from two plants of desirable quality(1/2) 2
9 Absorb phosphorous and transfer it to the plant(1) Glomus (1) 1+1 2
OR
Streptococcus (1) used as clot buster for removing blood clots from patients who have
suffered myocardial infarction.(1)
1+1
10 a. Commensalism(1/2) b. mutualism (1/2) c. competition(1/2) parasitism(1/2) ½X4 2
11 Unisexual flowers,dioecy,heterostyly, maturity of pollen and pistil at different times, self incompatibility( ½X4 3
any four)
Covering the flower with a paper bag/butter cup to prevent it from getting pollinated by unwanted
pollen.(1/2 m) Done twice( ½ m)( once after emasculation,then after pollinating with desired pollen)
½ +½
12 Duration of pregnancy, Parturition, placenta and fully developed foetus,Oxytocin, colostrum ½X6 3
13 Adaptive radiation in Marsupials(1) -A process of evolution of different species 3
in a given geographical area starting from a point and literally radiating to other
areas (1) Convergent evolution (1)
14 a. hn-RNA /heterogenous RNA (1/2) b. Hn RNA contains the coding sequences,-the exons 3
and the non coding sequences -the introns. The mature RNA has only the coding
sequences.(1) c.Splicing (1/2), capping (1/2), tailing (1/2)

15 Codon GAA and GAG code for the same amino acid, glutamic acid. The genetic code is 3
degenerate.(1)
GUG codes for valine.(1) Mutation B results in a change in the amino acid in the 6th position .
This results in a change in the shape to sickle shape(1)
16 S strain bacteria when injected – mice die; R- mice live; heat killed S – mice live; heat killed ½X4 3
S + live R – mice die; recovered living S from dead mice; R strain bacteria have been
transformed to S strain by the genetic material of heat killed S strain. 1
They discovered that the enzymes digesting the other molecules such as RNA,Proteins could
not affect transformation. Hence they concluded that DNA is the genetic material.
17 Cow is administered with FSH(1/2) ½X6 3
- induces follicular maturation and super ovulation(1/2)
- mating with an elite bull or artificial insemination(1/2)
- recovery of the fertilised egg at 8-32 celled stage(1/2)
- its transfer to the surrogate mother(1/2)
- Mother ready for another round of super- ovulation(1/2).
18 Benign tumor Malignant tumor 2+½
Restricted to one place Spreads/metastasis +½
Does not recur after surgery( mostly) Mostly recur after surgical removal
Not very harmful Lethal
Cancerous cells do not follow contact inhibition (1/2)
Interferons activates immune system and destroys tumor cells.(1/2)

19 a.to kill disease causing bacteria b. Lactobacillus c. remove clots from blood vessels ½X6
d. Cyclosporin A e. Beverage/medicines f. Propionibacterium sharmanii.
3
20 (a) EcoRI 3
1X3

(b)
(c) These are named sticky ends, because they form hydrogen bonds with their
complementary cut parts.
21 a) Curve ‘a’(1) b) exponential growth(1) carrying capacity(1) 1X3
22
3

2+1

(2m)
( numbers and weight if not mentioned can be ignored while giving marks)
Pyramid of number is inverted while pyramid of energy is
always upright as energy always flows from one trophic level to
the next.( 1)
23 a. alertness, responsibility and firmness in tackling a problem. He expresses boldness towards
environment consciousness.
b. Height of the smoke emitted from exhaust pipe is closer to the height of the children
c. It indicates that the vehicles comply with the new auto fuel policy. At present vehicles complying 1X4 4
Euro-IV or equivalent BS-IV norms are to ply in the identified 11 cities, rest of the country to follow
Euro III / BS-III norms.
d. In these devices, platinum - palladium and Rhodium are used as catalysts. As the exhaust passes
through the convertor, unburnt hydrocarbons are converted into CO2 and H2O. Carbon monoxide
and nitric oxide are changed into CO2 and N2 gas respectively. Vehicles fitted with such equipment
should run on unleaded petrol only since lead in petrol inactivates the catalysts
24 a. NCERT Text Page 52 Fig 3.11 b,c.d,e,f,g Diag(11/2) and correct labelling zygote,
morula,blastocyst(11/2)
b.After implantation, finger like projections appear on the trophoblast-chorionic villi(1)
-It is surrounded by uterine tissue and maternal blood(1/2)
-Villi and uterine tissue become interdigitated forming placenta(1/2) 5
OR
a. NCERT Text Page 26 Fig 2.8 (a) 1 ½ marks ,(b) 1 ½ marks (c) 1 mark
b.Only one of the megaspores of the tetrad is functional and it develops into the embryo sac
(1)
25 Green pod colour dominant and axial flowers are dominant.
a. GGAA X ggaa (1/2) Gametes GA ga (1/2) F1 GgAa (1/2)
F2 Punnet square(2), Phenotypic ratio 9:3:3:1, Law of independent assortment(1) 5m

OR
-The bacteriophage injects only the DNA into the bacterial cells and not the protein coat.(1)
- They made 2 different preparations of bacteriophage.In one, DNA was made radioactive
with P32 and in the other the protein was made radioactive with S35.(1)
-Phage allowed to infect bacteria.(1/2)
-Agitation in a blender to separate the viral particles from the bacteria.(1/2)
- Centrifugation to separate the viral coat and the bacteria. .(1/2)
Phages with radioactive protein donot transfer the radioactivity to the bacteria(1/2),
-phages with radioactive DNA transfer the radioactivity to the bacteria.(1/2)hence DNA is the
genetic material(1)
26 i. enzyme replacement, ii. bone marrow transplantation, iii. gene theray.( 1 1/2 m) 1 ½+½+3
gene isolate from the bone marrow of young embryos.(1/2 m)

MECHANISM OF GENE THERAPY:

i. DNA coding for ADA enzyme extracted From a healthy person. ii.Introduced into a
retrovirus where its cDNA produced(cDNA is complementary DNA) iii. This cDNA is then
introduced into the lymphocytes of the patient which are cultured outside his body.
iv.These lymphocytes with altered DNA are introduced into the bone marrow of the 5m
patient.v.Patient starts producing ADA enzyme on his own.
OR
To make the host accept the rDNA is called making it "competent". The problem in this
process is that DNA is Hydrophilic and cannot pass through the cell membrane which
allows fat soluble substances only. To overcome this problem, the following practices are
followed:
A) Bacteria are treated with divalent cations such as Ca2+ , which enables the rDNA to pass
through the membrane followed by heat shock method. By incubating the cells with rDNA on 1+4
ice and then exposing to heat shock (420) and putting them back on ice. This treatment
helps bacteria pick up the r DNA
B) Through micro injection method host can be made competent. In this directly the r DNA is
injected into the nucleus of animal cell.
C) In another method using biolistics or gene gun the DNA is coated on gold or tungsten micro-
pellets with high velocity and the plant cells are bombarded with these pellets.
D) Using disarmed pathogen: these are the pathogens which while infecting the cells, transfer
desirable rDNA into the host cell.

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