mAU(x100)

285nm,4nm (1.00)

6.5

6.0

5.5

5.0

4.5

4.0

3.5

3.0

2.5

2.0

1.5

1.0

0.5

0.0

0.0 2.5 5.0 7.5 10.0 12.5 min

inj no. name RT wavelength area area% height height% purity index
Baicalein 4.164 Ch3 285nm 2568378 45.9314 511611 2.218 1
Biochanin A 6.129 Ch3 285nm 1518207 27.1507 317663 2.673 1
1 Embelin 9.68 Ch3 285nm 965214 17.2613 192697 10.991 0.444834
Baicalein 4.183 Ch3 285nm 2528078 47.9089 511367 -- 1
Biochanin A 6.15 Ch3 285nm 1501902 28.4621 313416 2.628 1
2 Embelin 9.697 Ch3 285nm 951905 18.0393 187660 11.032 0.946527
Baicalein 4.149 Ch3 285nm 2585718 46.3799 514468 2.171 0.999997
Biochanin A 6.11 Ch3 285nm 1535277 27.5382 319671 2.709 1
3 Embelin 9.655 Ch3 285nm 972959 17.4519 192799 10.9 0.295389
Baicalein 4.141 Ch3 285nm 2594352 45.7925 531483 2.201 1
Biochanin A 6.097 Ch3 285nm 1527611 26.9636 326390 2.625 1
4 Embelin 9.645 Ch3 285nm 984597 17.379 194211 10.654 0.413939
Baicalein 4.143 Ch3 285nm 2556725 45.1589 523484 2.346 1
Biochanin A 6.096 Ch3 285nm 1520472 26.8558 323887 2.613 1
5 Embelin 9.645 Ch3 285nm 971207 17.1542 190747 10.697 0.27247
Baicalein 4.129 Ch3 285nm 2561079 45.3823 519624 2.251 1
Biochanin A 6.078 Ch3 285nm 1517487 26.8899 321416 2.691 1
6 Embelin 9.631 Ch3 285nm 963774 17.0781 187999 10.539 0.335599

average SD RSD
Baicalein 4.15 0.02 0.5
Biochanin 6.11 0.03 0.4
Embelin 9.66 0.02 0.3
tailing plate
1.182 12550.63
1.181 27511.84
1.175 63354.47
1.152 13056.65
1.173 27603.11
1.166 66099.21
1.161 12220.03
1.173 28063.47
1.171 66162.87
1.185 13099.55
1.175 28218.45
1.169 65557.75
1.179 12962.45
1.17 28049.2
1.17 65419.51
1.165 12884.21
1.169 28614.84
1.174 64408.51
 mAU(x1,000)
285nm,4nm (1.00)
1.6

1.5

1.4

1.3

1.2

1.1

1.0

0.9

0.8

0.7

0.6

0.5

0.4

0.3

0.2

0.1

0.0

0.0 2.5 5.0 7.5 10.0 12.5 min

sam no. inj no. name RT wavelengtharea area% height height% purity inde
Baicalein 4.214 Ch3 285nm 1081582 16.2046 153454 13.612 0.980649
1 Biochanin 6.002 Ch3 285nm 776625 11.6356 112039 11.024 0.999999
Baicalein 4.197 Ch3 285nm 1050495 15.9295 121730 5.702 0.981209
1 2 Biochanin 5.963 Ch3 285nm 766752 11.6269 88406 10.843 0.999998
Baicalein 4.213 Ch3 285nm 1857051 11.2207 257531 14.014 0.987153
1 Biochanin 5.999 Ch3 285nm 829657 5.013 113880 10.836 0.999999
Baicalein 4.199 Ch3 285nm 1782882 11.5313 292198 5.859 0.984557
2 2 Biochanin 5.98 Ch3 285nm 784086 5.0713 133982 10.76 0.999998
Baicalein 4.219 Ch3 285nm 1041329 56.201 177621 5.859 0.98225
1 Biochanin 6.014 Ch3 285nm 811536 43.799 145423 10.73 0.999999
Baicalein 4.196 Ch3 285nm 1028771 22.672 191659 6.592 0.978543
3 2 Biochanin 5.985 Ch3 285nm 863082 19.0206 158807 0.566 0.93279
Baicalein 4.196 Ch3 285nm 1624555 10.0556 298755 2.92 0.980455
1 Biochanin 5.976 Ch3 285nm 747895 4.6054 135554 0.68 0.929401
Baicalein 4.2 Ch3 285nm 1634505 10.0556 297744 2.922 0.980466
4 2 Biochanin 5.986 Ch3 285nm 748593 4.6054 135401 0.682 0.929434
Baicalein 4.215 Ch3 285nm 1443639 10.4865 259511 2.925 0.978936
1 Biochanin 6.004 Ch3 285nm 750763 5.4535 135635 0.54 0.9369
Baicalein 4.219 Ch3 285nm 1429196 10.5257 266013 3.12 0.978463
5 2 Biochanin 6.005 Ch3 285nm 740538 5.4539 135941 0.572 0.926735
Baicalein 4.233 Ch3 285nm 1998742 12.2673 374146 3.13 0.978245
1 Biochanin 6.03 Ch3 285nm 944913 5.7994 176902 0.634 0.866409

6
 Baicalein 4.22 Ch3 285nm 1966810 12.1845 379593 3.227 0.975869
6 2 Biochanin 6.016 Ch3 285nm 933926 5.7857 174735 0.635 0.876705

average SD RSD
Baicalein 4.21 0.01 0.3
Biochanin 6.00 0.02 0.3
plate tailing
10445.32 1.229
22655.76 1.034
10562.52 1.24
21583.12 1.243
10656.48 1.25
20785.75 1.029
10167.27 1.371
21154.78 1.176
10135.86 1.375
20909.83 1.182
11776.18 1.378
23403.85 1.123
10856.81 1.388
22688.4 1.08
10856.1 1.387
22688.48 1.084
11432.9 1.411
21312.45 1.079
11962.13 1.426
23434.69 1.099
12065.44 1.444
24349.05 1.142
12122.59 1.435
24535.86 1.138
 mAU(x100)
285nm,4nm (1.00)

2.25

2.00

1.75

1.50

1.25

1.00

0.75

0.50

0.25

0.00

0.0 2.5 5.0 7.5 10.0 12.5 min

sam no. inj no. name RT wavelengtharea area% height height%

1 Embelin 9.722 Ch3 285nm 1110630 68.7054 215552 10.742
1 2 Embelin 9.703 Ch3 285nm 984983 26.0064 174300 2.932
1 Embelin 9.686 Ch3 285nm 1126984 30.3781 200908 6.094
2 2 Embelin 9.703 Ch3 285nm 1009547 36.7722 174627 9.755
1 Embelin 9.749 Ch3 285nm 1181580 30.0401 199329 2.415
3 2 Embelin 9.756 Ch3 285nm 1202953 31.9438 200317 3.51
1 Embelin 9.738 Ch3 285nm 1121718 30.561 179237 3.72
4 2 Embelin 9.754 Ch3 285nm 1128102 30.3979 170283 1.211
1 Embelin 9.756 Ch3 285nm 1098795 29.8463 160983 3.27
5 2 Embelin 9.752 Ch3 285nm 1111030 32.448 155915 0.436
1 Embelin 9.748 Ch3 285nm 1053026 32.9568 145900 0.22
6 2 Embelin 9.745 Ch3 285nm 1091401 33.8937 150374 0.559
purity inde tailing plate
0.280509 1.172 64223.57
0.445131 1.116 52518.64
0.206241 1.122 54101.62
0.326721 1.12 50691.72
0.678757 1.098 49716.85
0.267511 1.106 48094.54
0.82079 1.084 44843.24
0.493463 1.085 40965.12
0.709502 1.085 38952.38
0.646094 1.079 36533.34
0.472459 1.091 35879.13
-0.07017 1.097 35870.01