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Determinationoflipids 1
Determinationoflipids 1
Determination of Lipids
Classification of lipids
I. Simple lipids: They are esters of fatty acids with various alcohols. They
include oils and fats which are esters of fatty acids with glycerol (i.e.
triglycerides).
II. Complex lipids: They contain (in addition to fatty acids and alcohols)
additional groups as phosphate (e.g. phospholipids), carbohydrates (e.g.
glycolipids) and proteins (e.g. lipoproteins).
III. Precursor and derived lipids: They include fatty acids, cholesterol,
steroid hormones, fat-soluble vitamins (vitamin A, D, E & K) and
2
BIOC 201 2010-2011 www.google.com
4. Lipoproteins (e.g. LDL & HDL) are a mean for transporting lipids in
blood.
B- Saponification of oils:
Note that the time for collection of soap solution is less than that for
collection of water because soap lowers the surface tension of water
making it flow easily through the syringe needle.
A- Acrolein test:
How to perform the test:
B- Denestan experiment:
How to perform the test:
VI- Cholesterol
A- Salkowski test:
How to perform the test:
B-Liebermann-Burchard test:
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