Confocal Microscope A1

Confocal Microscope
 — the ultimate confocal microscope

2 3
 Capturing high-quality images of cells and molecular events at high speed,
Nikon’s superior A1 confocal laser microscope series, with ground breaking technology,
enables you to bring your imaging aspirations to life.
A1 with high performance and A1R with additional high-speed resonant scanner
The new A1 series dramatically improves confocal performance and ease of operation. The A1R with a hybrid
scanner supports advanced research methods using photo activation fluorescence protein. The new ergonomic
user-friendly design facilitates live-cell work and a huge array of new imaging strategies.

Dynamics
A high-speed resonant scanner allows imaging of intracellular dynamics at 30 frames per
second (fps). Moreover, image acquisition of 230 fps is possible.

Interaction
Simultaneous imaging and photo activation with the proprietary hybrid scanner reveal
intermolecular interaction. Analysis software for FRAP and FRET is provided as standard.

Spectrum
Fast spectral image acquisition for 32 channels at a maximum of 16 fps is possible. New real-
time spectral unmixing and the V-filtering functions expand the range of use of spectral images.

Image Quality
Fluorescence efficiency is increased by 30 percent, and S/N ratio of images is also increased.
With diverse new technologies such as the VAAS pinhole unit, superior image quality has been
achieved.

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 Dynamics & Interaction
A1R’s hybrid scanner for ultrahigh-speed imaging and photo activation
A1R incorporates two independent galvano scanning systems: high-speed resonant and high-resolution
non-resonant. This allows ultrafast imaging and photo activation imaging required to unveil cell
dynamics and interaction.

Ultrahigh-speed imaging Optical path in the A1R

World’s fastest 230 fps (512 x 64 pixels) Optical output ports Continuous variable hexagonal pinhole (page 12)

A resonant scanner with ultrahigh resonance frequency of A detector port for the 4-PMT
7.8kHz is simultaneously mounted with a non-resonant detector, spectral detector port
scanner that is capable of high-resolution (4096 x 4096 pixels) and optional detector port is
image capture. incorporated. Resonant scanner
1D scanning 15,600 lps Resonant scanner Non-resonant scanner For high-speed imaging of 230 fps.
2D scanning 230 fps (512 x 64 pixels)
During photo activation imaging, it is
used for image capture.
Full frame scanning 30 fps (512 x 512 pixels)

Stable, high-speed imaging

The Nikon original optical clock generation method is employed for high-speed imaging with a resonant scanner.
Stable clock pulses are generated optically, offering images that have neither flicker nor distortion even at the highest speed.

High-speed data transfer with fiber-optic communication

The fiber-optic communication data transfer system can transfer data at a maximum of four giga bps—40 times faster than
the conventional method. This allows transfer of image data (512 x 512) in five modes at more than 30 frames per second. Excitation input ports
Up to seven lasers (maximum
nine colors) can be loaded.

High-speed imaging of wide field of view Non-resonant scanner

Low-angle incidence dichroic mirror (page 12)
When a non-resonant scanner is being used for high-speed image Wide field of view of resonant scanner For high-resolution imaging
acquisition, the field of view of the scanned image is reduced to avoid up to 4096 x 4096 pixels.
overheating of the scanner (motor). Resonant scanners do not suffer During photo activation
Field of view of non-resonant scanner
overheating. Therefore, the field of view of the scanned area is imaging, it is used for
approximately five times larger. stimulation.

What is a hybrid scanner?

High-speed photo activation imaging This mechanism allows flexible switching Photo activation
or simultaneous use of two galvano Imaging
scanners (resonant and non-resonant)
Simultaneous photo activation and imaging with high-speed hyper selector. High-speed imaging laser Resonant scanner

Simultaneous photo activation and fluorescence imaging is conducted using non-resonant and resonant scanners.
ch:1
Because the resonant scanner can capture images at 30 fps, image acquisition of high-speed biological processes 4000
Photo activation laser
3500
after photo activation is possible.
3000

High-speed imaging of photo activation T

2500
Hyper selector
2000

1500

1000
Hyper selector
500

0
50 100 150
Z(pixels)

Points within the cell and changes of

fluorescence intensity Non-resonant scanner
(From the point closer to the activated point:
Imaged at video rate (30 fps) while photo activating the point with a 405nm laser red, blue, violet)
33ms
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 Dynamics & Interaction
Applications with a hybrid scanner
The A1R’s high-speed imaging and hybrid scanners allow advanced imaging of cell dynamics and molecular interactions.

Ultrahigh-speed imaging and photo activation Photo conversion protein

High-speed imaging (high time resolution imaging) from a video rate of 30 fps (33ms time resolution) to 420 fps (2.4ms time resolution) Photo conversion protein is a fusion protein of the CFP variant and the PA-GFP variant. When the PA-GFP variant is activated with violet to
is possible. In addition, X-t scanning mode enables ultrahigh-speed imaging of dynamics with 64µs time resolution. Simultaneous photo ultraviolet light, it changes blue fluorescence to green fluorescence due to intermolecular FRET from CFP to PA-GFP.
activation during such high-speed imaging is also possible.
Observation with band scanning Observation with X-t scanning mode
Imaging at 420 fps (2.4ms/frame) Imaging with 64µs time resolution (15,600 lps)
Image size: 512 x 32 pixels
4000
3000
3500
2500 3000

2500 20ms
2000

20ms 2000
1500
1500
1000
1000

500 500
4000

Spot1
0 0 3000
10 20 30 40 50 500 1000 1500 2000 2000
Frame Line
1000
0/4000
HeLa cells expressing PA-GFP are excited with 488nm laser light. Directly after photo-activation (using 405nm laser light) of a region of interest, green emission (shown in grayscale) by photo- 3000

Spot2
activated PA-GFP is detected and the subsequent distribution of the photo-activated protein is recorded at high speed. Please note that photo-activation (with the 405nm laser) and image acquisition 2000
(with the 488nm laser) is performed simultaneously. Both XYt and Xt recordings are displayed. Graphs show fluorescence intensity (vertical) versus time (horizontal). 1000
Activation laser wavelength: 405nm, Imaging laser wavelength: 488nm 0/4000
3000

Spot3
Photos courtesy of: Dr. Tomoki Matsuda and Prof. Takeharu Nagai, Research Institute for Electronic Science, Hokkaido UniversityProf.
2000
1000
0/4000
Kaede photo conversion fluorescence protein 3000

Spot4
2000
1000
Kaede changes fluorescence colors irreversibly from green to red due to fluorescence spectral conversion when it is exposed to light with 0/4000
The graph indicates the changes of
3000

Spot5
a spectrum from ultraviolet to violet. 2000 fluorescence intensity in each ROI.
1000 The blue line indicates the changes of
2000 0 fluorescence intensity of the CFP
0 5 10
variant and the red line indicates the
Frame changes of fluorescence intensity of
1500
the PA-GFP variant.

1000 While imaging a HeLa cell expressing photo conversion protein with blue and green fluorescence using 457nm laser as excitation light, the PA-GFP variant in an ROI
is continuously activated with the 405nm laser. The activated part observed in blue fluorescence (shown in monochrome in the images) emits green fluorescence
500
(shown in red in the images). And the dispersion of photo conversion protein indicated by this green (shown in red in the images) is observed.
Activation laser wavelength: 405nm, Imaging laser wavelength: 457nm, Image size: 512 x 512 pixels, 1 fps
Photos courtesy of: Dr. Tomoki Matsuda and Prof. Takeharu Nagai, Research Institute for Electronic Science, Hokkaido University
0
While imaging a HeLa cell expressing Kaede with green and red fluorescence using 488nm and 561nm lasers as excitation lights, Kaede in a ROI is continuously 2 4 6 8
Frame
activated with the 405nm laser for photo conversion. The dispersion of Kaede red fluorescence produced by photo conversion is observed. The horizontal axes of
two graphs indicate time and the vertical axes indicate fluorescence intensity (pixel intensity). The green line and red line in the graph respectively indicate
intensity change of Kaede green and red fluorescence in the ROI.
Activation laser wavelength: 405nm, Imaging laser wavelength: 488nm/561nm, Image size: 512 x 512 pixels, 1 fps
Four-color imaging
Photos courtesy of: Dr. Tomoki Matsuda and Prof. Takeharu Nagai, Research Institute for Electronic Science, Hokkaido University

Standard four-channel detector eliminates the necessity of an additional fluorescence

FRET (Förster Resonance Energy Transfer) detector after purchase and allows easy imaging of a specimen labeled with four probes.

FRET is a physical phenomenon that occurs when there are at least two fluorescent molecules within a range of approximately 10nm. When the
emission spectrum of a fluorescent molecule overlaps with the absorption spectrum of another fluorescent molecule and the electric dipole directions
of the two molecules correspond, radiationless energy transfer from a donor molecule to an acceptor molecule may occur.

2000

1500

1000

HeLa cells expressing Yellow Cameleon 3.60 were excited with 457nm laser light. After stimulation with histamine, calcium ion concentration dynamics were 500
observed. The (blue) emission of CFP and the (yellow) emission of YFP are shown as green and red channels respectively. The graph displays fluorescence Image of a zebra fish labeled with four probes
intensity (vertical) versus time (horizontal). The green and red lines in the graph indicate the intensity change of CFP emission (green) and YFP emission (red) Nucleus (blue): Hoechst33342, Pupil (green): GFP, Nerve (yellow): Alexa555, Muscle (red): Alexa647
from the region of interest (ROI). Along with the increase of calcium ion concentration in the cell, the intermolecular FRET efficiency between CFP and YFP within 0 Photographed with the cooperation of: Dr. Kazuki Horikawa and Prof. Takeharu Nagai,
Yellow Cameleon 3.60 increases, the CFP fluorescence intensity decreases, and the YFP fluorescence intensity increases. 10 20 30 40 50 60 Research Institute for Electronic Science, Hokkaido University
8 Imaging laser wavelength: 457nm, Image size: 512 x 512 pixels, 30 fps Frame 9
Photos courtesy of: Dr. Kenta Saito and Prof. Takeharu Nagai, Research Institute for Electronic Science, Hokkaido University
 Spectrum
Enhanced spectral detector
Nikon’s original spectral performance is even further enhanced in the A1 series, allowing high-speed spectral
acquisition with a single scan. In addition, new functions including a V-filtering function are incorporated.

Optical fiber
The wavelength resolution is
Fast 32-channel imaging at 16 fps DEES system independent of pinhole diameter.
High diffraction efficiency is achieved by
New signal processing technology and high-speed AD conversion circuit allow acquisition of a 32-channel matching the polarization direction of
Unpolarized light
spectral image (512 x 512 pixels) in 0.5 second. Moreover, acquisition of 512 x 64 pixels images at 16 frames light entering a grating to the polarizing
per second is realized. Polarized beam splitter
light beam S.
S2

Polarization rotator P S1

Faster spectral unmixing S1

S2
Nikon’s original algorithms and high-speed data processing enable fast and accurate unmixing during image
acquisition in less than a second. Coupled with high-speed spectral imaging, an image with no crosstalk can
be created in real time.

0.75

0.5

32-channel detector
0.25

Multiple gratings A precisely corrected 32-PMT array detector

is used. A three-mobile-shielding
Wavelength resolution can be varied
0 mechanism allows simultaneous excitation
400 450 500 550 600 650 700 between 2.5/6/10nm with three gratings.
by up to four lasers
Specimen: HeLa cell Each position is precisely controlled for
Fluorescence reagent: anti-tubulin/Alexa488 (microtubule), Histone H2B-GFP (chromosome) high wavelength reproducibility.
Specimen courtesy of: Dr. Tokuko Haraguchi, Kobe Advanced ICT Research Center, NICT

Simultaneous excitation of four lasers

High-quality spectral data acquisition
Three user-defined laser shields allow simultaneous use of four lasers selected from a maximum of nine
colors, enabling broader band spectral imaging. Diffraction Efficiency Enhancement System (DEES) High-efficiency fluorescence transmission technology
With the DEES, unpolarized fluorescence light emitted by the specimen is The ends of the fluorescence fibers and detector surfaces use a proprietary anti-
separated into two polarizing light beams P and S by a polarizing beam reflective coating to reduce signal loss to a minimum, achieving high optical
splitter. Then, P is converted by a polarization rotator into S, which has higher transmission.
diffraction efficiency than P, achieving vastly increased overall diffraction
V-filtering function freely utilizes 32 channels efficiency. Accurate, reliable spectral data: three correction techniques
Characteristics of grating Three correction techniques allow for the acquisition of accurate spectra:
100 interchannel sensitivity correction, which adjusts offset and sensitivity of each
With the V-filtering function, up to four desired spectral ranges can be selected from 32 channels and total channel; spectral sensitivity correction, which adjusts diffraction grating spectral
90
intensity of each range is adjusted individually, as if separating colors and controlling four PMTs by using Diffraction efficiency (%)
efficiency and detector spectral sensitivity; and correction of spectral transmission
80 S polarizing light beam
optical filters. It allows acquisition of the desired spectral range, providing flexibility to handle any new of optical devices in scanning heads and microscopes.
70
fluorescence probes.
60 Multi-anode PMT sensitivity correction
50 (Brightness) Pre-correction (Brightness) Post-correction
4000 4000
40
30 P polarizing light beam 3500 3500

Up to four wavelength ranges 20 3000 3000

are selectable. 10 2500 2500

0 2000 2000
The intensity of each wavelength 1 4 7 10 13 16 19 22 25 28 31 1 4 7 10 13 16 19 22 25 28 31
400 Wavelength (nm) 750 (Channel) (Channel)
range is adjustable.

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 Image Quality
Key Nikon innovations for improving image quality
A best ever image quality is realized by an increased light sensitivity resulting from comprehensive technological
innovations in electronics, optics and software.

Low-angle incidence dichroic mirror realizes 30% increase in fluorescence efficiency VAAS pinhole unit transcends the existing concept of a confocal microscope
With the A1series, the industry’s first low-angle incidence method is employed on dichroic mirrors. It is commonly known that reducing pinhole size to eliminate flare light from non-focal plane causes darker images.
High transmission rate of an average 98% and a 30% increase of fluorescence efficiency are realized. The VAAS (Virtual Adaptable Aperture System) provides a new confocal microscopy that can eliminate flare while
retaining image brightness.
Low-angle incidence method
Increased fluorescence efficiency
45º incidence angle method
100 Principle and features
Conventional 45º Low-angle
incidence angle method incidence method 90
80 Conventional confocal microscope VAAS pinhole unit
Transmission rate (%)

70 Small pinholes reduce flare By the deconvolution of the

but darken images, while light that passes through the
60
large pinholes brighten pinhole and the light that
50 images but increase flare. doesn’t pass through the
Focal plane pinhole, flare can be eliminated Focal plane
40
Reflection-transmission Reflection-transmission
characteristics have high characteristics have lower 30
while using a large pinhole.
polarization dependence polarization dependence
20
10
0
380 430 480 530 580 630 680 730 780
Comparison of fluorescence efficiency

Brighter images with continuous variable hexagonal pinhole

Light that doesn’t pass Light that doesn’t pass
through the pinhole is through the pinhole is
Instead of a continuous variable square pinhole, the industry’s first hexagonal pinhole is employed. High brightness not used. also used.
equivalent to that of an ideal circular pinhole can be achieved while maintaining the confocality.
Square pinhole Hexagonal pinhole Light that passes through the
Light that passes through the
pinhole is detected. pinhole is detected.
30% more light
Effects
1 Acquisition of brighter images with less flare is possible.
2 Different sectionings (slice thicknesses) can be simulated via deconvolution after image acquisition.
64% of the area of a circle 83% of the area of a circle
3 Images of both focal plane and non-focal plane can be acquired with a single scan, boosting speed and reducing damage to live cells.

Conventional confocal microscope image

DISP improves electrical efficiency Minimum pinhole Maximum pinhole VAAS pinhole unit image
Unblurred but dark image Bright but blurred image Bright and unblurred image
Nikon original DISP (Dual Integration Signal Processing) technology has been implemented in the image processing circuitry
to improve electrical efficiency, preventing signal loss while the digitizer processes pixel data and resets. The signal is
monitored for the entire pixel time resulting in an extremely high S/N ratio. Flare

DISP

Integrator (1)

Integrator (2)

Pixel time
Integration Hold Reset

Two integrators work in parallel as the optical signal is read to ensure there are no gaps. Photographed with the cooperation of: Dr. Yasushi Okada, Cell Biology, Medical Dept. of Graduate School, Tokyo University
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 Easy to Use
Increased flexibility and ease of use
Control software NIS-Elements C features easy operation and diverse analysis functions. Combined with a
remote controller and other hardware, it provides a comprehensive operational environment.

NIS-Elements C 4-channel detector unit with changeable filters

Superior operability based on analysis of every possible confocal microscope operation pattern realizes easy operation without In combination with four lasers, simultaneous observation of four
an instruction manual, satisfying both beginners and experienced confocal users. Taking advantage of the hybrid scanner, it fluorescence labels is possible as standard. Each of three filter wheels
enables a complicated sequence of experiments such as photo activation to be carried out with simple setting and operation. can mount six filter cubes that are commonly used for a microscope, and
are all easily changeable by users. This combines modularity, flexibility
with user friendliness.
Simple image acquisition
･ Basic operation ･ Optical setting
Parameters for basic image acquisition are integrated in a single By simply selecting a fluorescence probe, an appropriate filter and laser
window, allowing simple image acquisition. wavelength are set automatically. Microscope setup is also conducted
automatically.

Spline Z scans for real-time display of cross-sectional images

High-speed image acquisition in the Z direction as well as the XY
direction is possible. By using the piezo motorized Z stage, arbitrary
vertical cross-sectional view can be achieved in real time without
acquiring a 3D image.

Easy operation by remote controller

Diverse application The remote controller allows the regulation of major settings of laser,
detector, and scanner with simple operation using push buttons and
･ Parameter setting for photo activation ･ Multidimensional image acquisition dials.
Timing and imaging parameters for Acquisition of images with a free
photo activation are set intuitively. combination of multidimensional
parameters including X, Y, Z, t and λ is
possible.

CLEM (Controlled Light-Exposure Microscopy)

During lengthy time-lapse imaging, cellular apoptosis caused by the exposure to light is a problem. The CLEM senses
the fluorescence signals and controls the on/off of the laser exposure depending on signal intensity. This reduces laser
exposure and alleviates the problem of cellular apoptosis.
Non-CLEM: Apoptosis occurs after a lapse of one hour
Reliable analysis functions
Real-time ratio display
Deconvolution
10 µm
High-speed 3D rendering
0 min 27 min 96 min 169 min
Multidimensional image display (nD Viewer)
Synchronized display of multidimensional images (View CLEM: Apoptosis doesn’t occur even after a lapse of 2.5 hours
synchronizer) Expression GFP in Histone 2B of HeLa cell
Diverse measurement and statistical processing Excitation laser wavelength: 488nm, Fluorescence acquisition
wavelength: 500-530nm, Single image acquisition time: 4
Powerful image database function seconds, Intervals: 1 minute, Total acquisition time: 3 hours
Colocalization and FRET Photos courtesy of: Dr. Merel Adjobo-Hermans, Department of Cell Biology,
0 min 27 min 96 min 169 min Amsterdam University, The Netherlands

14 15
 System components Recommended objective lenses
CFI Plan Apochromat 10x NA 0.45, W.D. 4.0mm
CFI Plan Apochromat 20xVC NA 0.75, W.D. 1.0mm NEW
Laser unit Detector unit Filter Cubes CFI Plan Apochromat 40xC NA 0.95, W.D.0. 14mm
LU-LR 4-laser CFI Plan Apochromat VC 60xWI NA 1.20, W.D. 0.27mm
Power Source Rack
CFI Apochromat TIRF 60x NA 1.49, W.D. 0.13mm
CFI Apochromat TIRF 100x NA 1.49, W.D. 0.12mm
CFI Plan Fluor 10x NA 0.30, W.D. 16.0mm
AOM
Filter Wheel
Unit A1 Scanning Head 4-detector Unit CFI Plan Fluor 20x NA 0.50, W.D. 2.1mm
for VAAS
CFI Plan Fluor 40x NA 0.75, W.D. 0.66mm
CFI S Fluor 10x NA 0.50, W.D. 1.2mm
Filter Cubes
Option CFI S Fluor 20x NA 0.75, W.D. 1.0mm
CFI S Fluor 40x NA 0.90, W.D. 0.3mm
A1-DUS Spectral Detector Unit A1-DU4 4-detector Unit CFI S Fluor 40x Oil NA 1.30, W.D. 0.22mm
CFI S Fluor 100x Oil with Iris diaphragm NA 0.5-1.30, W.D. 0.20mm

L4 L3 L2 L1
A1R Scanning Head Spectral Detector Unit Recommended filters
Excitation laser Channel 1 Channel 2 Channel 3 Channel 4
405/488/561/638 450/50 525/50 595/50 700/75
C-LU3EX 3-laser Unit EX LU4 4-laser Unit
405/488/543/638 450/50 515/30 585/65 700/75
457/514 482/35 540/30 — —
For filters other than the above, please consult your local Nikon representative.

A1R scanner set/A1 standard scanner set 4-laser Unit Diascopic Detector Unit

Controller

Scanning Head

Remote Controller
Either A1 standard scanner set or A1R scanner set can be chosen. 4-laser Power Source Rack

Microscope 90i/FN1 Adapter Set

Y-IDP-A1
D-DH-E-A1
Double Port 0/100
Software

A1-TI Ti Adapter Set A1-TE TE Adapter Set A1-FN1 FN1 Adapter Set A1-90I 90i Adapter Set

3-laser Unit EX

Ti-E TE2000-E FN1 90i PC

Z-focus Module

A1-DUT Diascopic Detector Unit

16 17
 Specifications

Diverse peripherals and systems for pursuit of live cell imaging A1R A1
Input/output port Laser input port: 3 (FC x2, direct x1)
Signal output port: 4 (SMA x2, FC x1, VAAS x1)
CFI Plan Apochromat VC series objectives Laser Wavelength and power 405LD: max. 38mW, Multi-Ar (457/488/514): max. 65mW, 488DPSS: max. 75mW, 561DPSS: max. 25mW, 543HeNe: max.
The VC lens corrects aberrations up to the viewfield periphery 1mW, 638LD: max. 10mW
and eliminates shading, providing uniform high resolution 440LD: max. 15mW (available as option)
throughout the viewfield. Axial chromatic aberration is corrected
up to 405nm (h line), making this series perfect for confocal Modulation Method: AO (Acousto) device or drive current control
observations and photo activation with a semiconductor laser. Control: power control for each wavelength, Return mask, ROI exposure control
The frequently used 20x objective has been added recently to this Laser unit Standard: LU4 4-laser unit
series.
Optional: C-LU3EX 3-laser unit EX
Standard fluorescence detector Wavelength 485-650nm
Detector 4 PMT
Filter cube 6 filter cubes commonly used for a microscope mountable on each of three filter wheels
Recommended wavelengths: 450/50, 482/35, 525/25, 595/50, 700/75, 540/30, 515/30, 585/65
Diascopic detector Wavelength 440-700nm
CFI Plan Apo VC 100x Oil, NA 1.40
CFI Plan Apo VC 60x Oil, NA 1.40 Detector PMT
CFI Plan Apo VC 60x WI, NA 1.20
CFI Plan Apo VC 20x, NA 0.75 (NEW)
Scanning head Scanning Scanning range: square inscribed in a ø18mm circle
Standard image acquisition Standard image acquisition
Scanner: non-resonant scanner x2 Scanner: non-resonant scanner x2
Confocal microscope with Perfect Focus System Multi-mode imaging system—A1 with TIRF system Pixel size: max. 4096 x 4096 pixels Pixel size: max. 4096 x 4096 pixels
With the inverted microscopes Ti-E and TE2000, an automatic focus The laser TIRF system and the confocal microscope system A1 series can be Scanning speed: 4 fps (512 x 512 pixels) Scanning speed: 4 fps (512 x 512 pixels)
maintenance mechanism—Perfect Focus System (PFS) can be used. It mounted simultaneously on the inverted microscope Ti-E or TE2000-PFS. Zoom: 1-1000x continuously variable Zoom: 1-1000x continuously variable
continuously corrects focus drift during long time-lapse observation and The laser TIRF system incorporates an epi-fluorescence module. Switching Scanning mode: X-Y, XY rotation, Free line, Line Z Scanning mode: X-Y, XY rotation, Free line, Line Z
when adding reagents. and adjustment of alignment is easy between the two light sources. High-speed image acquisition
By combining the observations of single molecules with laser TIRF and the
Scanner: resonant scanner (X-axis, resonance frequency 7.8kHz),
sectioning capabilities of the A1, this system
allows for multi-perspective cellular analysis. non-resonant scanner (Y-axis)
Pixel size: max. 512 x 512 pixels
Concept of the Perfect Focus System Scanning speed: 30 fps (512 x 512 pixels) to 230 fps
(512 x 64 pixels),
Specimen 15,600 lines/sec (line speed)
Interface Zoom: 7 steps (1x, 1.5x, 2x, 3x, 4x, 6x, 8x)
Coverslip Motorized PFS nosepiece CFI Apochromat TIRF 60x Oil, NA 1.49 (left) Scanning mode: X-Y, Line
CFI Apochromat TIRF 100x Oil, NA 1.49 (right)
Oil, water (inverted microscope Ti-E) Acquisition method: Standard image acquisition,
Objective
Perfect Focus Nosepiece High-speed image acquisition, Simultaneous photo activation
LED and image acquisition
Near-IR light
Line-CCD Dichroic mirror Low-angle incidence method
Position: 8
Offset lens
Observation
Standard filter: 405/488, 405/488/561, 405/488/561/638, 405/488/543/638, 457/514, BS20/80
light path Pinhole 12-256µm variable (1st image plane)
Camera
Spectral detector Number of channels 32 channels
The diagram shows the case when an immersion type objective is used.
A dry type objective is also available.
Spectral image 4 fps (256 x 256 pixels), 1000 lps
acquisition speed
Maximum wavelength 80nm (2.5nm), 192nm (6nm), 320nm (10nm)
Motorized stages Stage incubation system INU series and resolution Wavelength range variable in 0.25nm steps
Motorized stage makes multipoint observation easy. It allows multipoint Temperature of the stage, water bath, cover, and objective lens is
XYt (4D), multipoint XYZ (4D), multipoint XYZt (5D) and multipoint XYZtλ controlled, allowing living cells to be maintained for a long period. A Unmixing High-speed unmixing, Precision unmixing
(6D, including spectral information) observations. By using the standard transparent glass heater prevents condensation, and focus drift due to heat Z step 0.025µm
motorized stage or motorized XY stage equipped with a linear encoder with expansion on the stage surface is prevented, making this system ideal for
Compatible microscopes ECLIPSE Ti-E inverted microscope,
enhanced positioning repeatability in combination with the optional lengthy time-lapse imaging applications.
motorized piezo Z stage with high-speed Z-direction scanning capability, Manufactured by Tokai Hit Co., Ltd. ECLIPSE TE2000-E inverted microscope,
high-speed line Z scans are possible. ECLIPSE 90i upright microscope,
ECLIPSE FN1 fixed stage microscope
Option Motorized XY stage, High-speed Z stage, VAAS, CLEM
Software Display/image generation 2D analysis, 3D volume rendering/orthogonal, 4D analysis, spectral unmixing
Image format JP2, JPG, TIFF, BMP, GIF, PNG, ND2, JFF, JTF, AVI, ICS/IDS
Application FRAP, FLIP, FRET, photo activation, three-dimensional time-lapse imaging, multipoint time-lapse imaging, colocalization
Control computer OS Microsoft Windows® XP 32bit SP (English version)
CPU Intel Xeon 5160 (3GHz/1333MHz/dual core) or higher
Standard motorized XY stage Memory 4GB or more
Hard disk SAS (15,000rpm), 160GB or more x2, RAID 0 configuration
Data transfer Dedicated data transfer I/F
Monitor 1600 x 1200 or higher resolution, 2 LCD monitor configuration recommended
Motorized Piezo Z stage
Installation condition Temperature 5 to 35°C, humidity 65% (RH) or less (non-condensing)
18 19
 Layout Unit: mm

Scanning Head Remote Controller PC＋Monitor

4-detector Unit

Spectral Detector Unit

4-laser Unit

1150
EMISSION POWER
○

4-laser Power Controller

Supply Rack 2990

Dimensions and weight

LU4 4-laser unit 438(W) x 301(H) x 690(D)mm Approx. 35kg (without laser)

LU-LR 4-laser power source rack 438(W) x 400(H) x 800(D)mm Approx. 20kg (without laser power source)
Scanning head 276(W) x 163(H) x 364(D)mm Approx. 13kg

Controller 360(W) x 580(H) x 600(D)mm Approx. 40kg

A1-DU4 4-detector unit 360(W) x 199(H) x 593.5(D)mm Approx. 16kg

Power source

Controller Input voltage: 100–240VAC ±10％ 50–60Hz

Current rating: 5A @100VAC The AOTF incorporated into the 4-laser unit
Overcurrent protection: main breaker 15A and the AOM optionally incorporated into
the 3-laser unit are classified as controlled
LU-LR 4-laser power source rack Power source for Ar laser and control circuit: 100VAC, products (including provisions applicable to
controlled technology) under foreign
15A/115VAC, 15A/230VAC, 7.5A, 50/60Hz (breaker 15A) exchange and trade control laws. You must
Power source for lasers except Ar laser: 100VAC, 3A/115VAC, obtain government permission and
complete all required procedures before
3A/230VAC, 1.5A, 50/60Hz (breaker 5A) exporting this system.

Specifications and equipment are subject to change without any notice or obligation
on the part of the manufacturer. February 2008 ©2008 NIKON CORPORATION
TO ENSURE CORRECT USAGE, READ THE CORRESPONDING
WARNING MANUALS CAREFULLY BEFORE USING YOUR EQUIPMENT.
* Monitor images are simulated.
Company names and product names appearing in this brochure are their registered trademarks or trademarks.

NIKON CORPORATION
6-3, Nishiohi 1-chome, Shinagawa-ku, Tokyo 140-8601, Japan
phone: +81-3-3773-8973 fax: +81-3-3773-8986
http://www.nikon-instruments.jp/eng/

NIKON INSTRUMENTS INC. NIKON SINGAPORE PTE LTD NIKON UK LTD.

1300 Walt Whitman Road, Melville, N.Y. 11747-3064, U.S.A.
phone: +1-631-547-8500; +1-800-52-NIKON (within the U.S.A.only)
fax: +1-631-547-0306
http://www.nikoninstruments.com/
NIKON INSTRUMENTS EUROPE B.V.
Laan van Kronenburg 2, 1183 AS Amstelveen, The Netherlands
phone: +31-20-44-96-222 fax: +31-20-44-96-298
http://www.nikoninstruments.eu/
NIKON INSTRUMENTS (SHANGHAI) CO., LTD.
CHINA phone: +86-21-5836-0050 fax: +86-21-5836-0030
(Beijing branch) phone: +86-10-5869-2255 fax: +86-10-5869-2277
(Guangzhou branch) phone: +86-20-3882-0552 fax: +86-20-3882-0580
Printed in Japan (0802-00)T
SINGAPORE phone: +65-6559-3618 fax: +65-6559-3668 UNITED KINGDOM phone: +44-20-8541-4440 fax: +44-20-8541-4584
NIKON MALAYSIA SDN. BHD. NIKON GMBH AUSTRIA
MALAYSIA phone: +60-3-7809-3688 fax: +60-3-7809-3633 AUSTRIA phone: +43-1-972-6111-00 fax: +43-1-972-6111-40
NIKON INSTRUMENTS KOREA CO., LTD. NIKON BELUX
KOREA phone: +82-2-2186-8410 fax: +82-2-555-4415 BELGIUM phone: +32-2-705-56-65 fax: +32-2-726-66-45
NIKON CANADA INC.
CANADA phone: +1-905-625-9910 fax: +1-905-625-0103
NIKON FRANCE S.A.S.
FRANCE phone: +33-1-45-16-45-16 fax: +33-1-45-16-00-33
NIKON GMBH
GERMANY phone: +49-211-9414-0 fax: +49-211-9414-322
NIKON INSTRUMENTS S.p.A.
ITALY phone: +39-55-3009601 fax: +39-55-300993
NIKON AG
SWITZERLAND phone: +41-43-277-2860 fax: +41-43-277-2861
En
Code No.2CE-SBTH-1 This brochure is printed on recycled paper made from 40% used material.