BIOL1362 Enzymes tutorial

1. i) Briefly describe what is meant by competitive enzyme inhibition

ii) Briefly describe how this type of inhibition is influenced by relative concentrations of inhibitor
and substrate

iii) Briefly explain how this type of inhibition differs from uncompetitive inhibition

2. An enzyme which obeys Michaelis-Menton kinetics was found to be inhibited by compound A.

The following results were obtained at a fixed total enzyme concentration:

Substrate conc Initial velocity (µmol product formed per minute)

(mmol/L) 1/[S] Inhibited (vo) 1/(vo) With 1mmol l A (vio) 1/(vio)
12.5 0.08 0.252 3.968 - -
18 0.056 0.333 3.003 0.286 3.497
25 0.04 0.416 2.404 0.357 2.801
50 0.02 0.625 1.6 0.541 1.848
66.6 0.015 0.714 1.401 0.617 1.621
100 0.01 0.833 1.2 0.714 1.401
200 0.005 0.666 1.502 - -

i) In both cases, derive the Vmax and Km

ii) What kind of inhibitor is compound A and how might it bind
iii) Comment on both sets of results

3. Explain the effects of pH on enzyme activity

4. List four ways in which enzyme activity may be regulated without changing enzyme
concentration