Professional Documents
Culture Documents
Applications
GC and GC-MS
Applications
Ion Chromatography
Applications
Liquid Chromatography
Applications
BeerNHop Solutions
Once a manufacturer has developed a popular product they need to ensure that they can reproduce
Ion Chromatography
it consistently, and in sufficient quantity, in compliance with any local & national regulations. It also
Applications needs to remain stable over the shelf-life of the beer.
Brewers have to ensure that raw ingredients are consistent from batch to batch and methodology
Liquid Chromatography
is available to detect minute differences in the raw ingredients as it can affect taste, color and the
Applications shelf life of the product. Raw ingredient contamination can also result in a food safety issue. Results
need to be available quickly so there are no unforeseen delays in the brewing process as these can
BeerNHop Solutions be costly to the business. Rapid analysis methods are also required during the brewing process to
ensure the process is going according to set standards. Failure to monitor various critical parameters
Peer Reviewed Journal References during the brewing process can lead to product recalls, vast wastage of the product or down time
to the brewing process. The final beer is tested to ensure it meets all quality and labelling criteria to
comply with local and national regulations.
Thermo Scientific™ chromatography systems and photometric analyzers offer distinct benefits
throughout the beer brewing analysis process. Using these systems and our highly sensitive and
selective detectors, state-of-the-art column technologies, along with the proven analytical methods
presented here, will help you to:
• Monitor isomerization and decomposition progress during and after brewing to determine
flavor and stability
• Maintain and preserve essential flavoring components such as bitterness and smoothness
• Use metabolomic approaches to characterize your beer sample, monitor the fermentation
process and study product stability
Table of Contents
Automated Discrete Photometry
Complete Brewing Solutions
Automated Discrete Photometry Fast and effective quality monitoring for breweries
Applications
Over 40 years experience in designing and manufacturing automated analyzers established the
ability to measure wine and water and recently progressed to testing beer, wort and malt. Discrete
GC and GC-MS
photometric analyzers provide excellent analytical performance for colorimetric and enzymatic
Applications testing. Their automated operating systems allow laboratories to simultaneously measure multiple
analytes while reducing total analysis time and increasing efficiency. Thermo Scientific™ Gallery™ Plus
Ion Chromatography Beermaster, launched in 2011, is a new generation analyzer available in a bench-top compact system
Applications that is reliable and user-friendly. In addition to over 30 beer, wort and malt tests, the analyzer can
measure water and several other parameters, like enzyme activity, measurements often required by
manufactures. Gallery Plus Beermaster can analyze bitterness, SO2, beta-glucan, free amino nitrogen,
Liquid Chromatography
beer color, sugars, pH, acetaldehyde and acids quickly and accurately. This analysis requires no
Applications extra sample preparation with the exception of degassing for carbonated samples. With a maximum
capacity of 350 photometric tests per hour, the analyzer is very straightforward to use requiring only
BeerNHop Solutions minimal training to run routine analysis and provide maintenance. Use of this automated system
increases efficiency in quality control, reduces costs, and improves productivity in breweries.
Peer Reviewed Journal References
• Multiple colorimetric tests can be run simultaneously
Applications
GC and GC-MS
Applications
Ion Chromatography
Applications
Discrete Photometry for Beer Analysis
Liquid Chromatography
Applications
Download Poster Note: Automated Wort and Beer Quality Control Analysis
Table of Contents
Discrete Photometry for Beer Analysis
Complete Brewing Solutions
250
Measured (mg/L)
200
150
100
50
0
0 50 100 150 200 250 300
FIA (mg/L)
Figure 1. Method comparison showing wort samples (red) and beer samples (black).
Download Poster Note: Rapid Determination of High Molecular Weight 1,3/1,4-Beta-D-Glucan by a Novel Photometric Method
Table of Contents
Discrete Photometry for Beer Analysis
Complete Brewing Solutions
Automated Discrete Photometry Free Amino Nitrogen (FAN) Analysis using NOPA method
Applications
In the fermentation process that produces beer, yeast (usually Saccharomyces) converts sugars to
ethanol and carbon dioxide. This yeast synthesizes the proteins required for healthy growth from
GC and GC-MS
amino acids, created by yeast from ammonia or by removal of the amino group from other alpha
Applications amino acids. The alpha amino acids available to the yeast in fermentation are known as FAN.
The ninhydrin method measures the content of ammonia in addition to the FAN equaling the total
Ion Chromatography assimilable nitrogen. A rapid 2-reagent NOPA (alpha-amino nitrogen by OPA) method was developed
Applications for the automated discrete analyzer using a blank buffer to eliminate sample color interference. Total
analysis time for six samples and 60 test requests was approximately 45 minutes. The NOPA method
showed excellent repeatability between 2.2 and 3.2 %. In this example the correlation between beer
Liquid Chromatography
and wort samples is measured according to the EBC FAN protocol and the NOPA method.
Applications
250
200
150
100
50
0
0 50 100 150 200 250 300
FAN Result (mg/L)
Figure 2. Method comparison. The two wort samples are the ones with highest NOPA concentration.
Download Poster Note: Beer Reference Sample Correlation between Free Amino Nitrogen (FAN) and NOPA (Nitrogen by OPA)
Table of Contents
Discrete Photometry for Beer Analysis
Complete Brewing Solutions
BeerNHop Solutions Linearity 2-50 mg/L Beer 8 5.3 4.4 0.9 1.4
60
y = 1.0248x + 0.1289
R² = 0.99974 Beer 9 3.5 2.7 0.8 1.1
Peer Reviewed Journal References 50
30
Beer 11 1.2 0.8 0.4 1.4
Download Poster Note: Correlation of Automated Total SO2 DTNB Method to EBC/ASBC Para-rosalinine Methods
Table of Contents
Discrete Photometry for Beer Analysis
Complete Brewing Solutions
200
Lager 3 110 108 98
Result (mg/l)
150
0
50 70 90 110 130 150 170 190 210 Bitter 1 195 207 106
Theoretical (mg/l)
Figure 4. Linearity.
Bitter 2 142 133 94
Download Poster Note: Evaluation of a Gallery Total Polyphenol Method Performance in Beer (and Wort) using the EBC/MEBAK Protocol
Table of Contents
Discrete Photometry for Beer Analysis
Complete Brewing Solutions
BeerNHop Solutions
y = 0.9302x + 0.6381
Linearity y = 0.9226x + 0.47 Method comparison
R2= 0.99974 R2= 0.99913
140 140
Peer Reviewed Journal References
120 120
100 100
Gallery (EBC)
Gallery (EBC)
80 80
60 60
40 40
20 20
0 0
0 20 40 60 80 100 120 140 0 20 40 60 80 100 120 140
Theoretical value (EBC) Manual spectrophotometer (EBC)
Download Poster Note: Gallery Method Performance of Beer (and Wort) Color Measurement using the EBC protocol
Table of Contents
GC and GC-MS
Complete Brewing Solutions
GC instruments from Thermo Scientific provide outstanding performance for routine analyses,
Ion Chromatography
advanced capabilities, and the flexibility to increase sample throughput. Instant connect injectors and
Applications detectors enable you to change modules in minutes to reconfigure the instrument for new workflows,
develop new methods and eliminate maintenance downtime. Current developments in GC-MS triple
Liquid Chromatography quadrupole technology deliver high sensitivity and selectivity in the small molecule mass range and
Applications
allow the detection of compounds at low concentrations, even in complex matrix samples. A simple
and standard approach using electron impact ionization (EI) enables a very straightforward method for
low-level analysis, such as that of dioxins.
BeerNHop Solutions
Applications
GC and GC-MS
Applications
Ion Chromatography
Applications
GC and GC-MS for Beer Analysis
Liquid Chromatography
Applications
Vicinal Diketones
BeerNHop Solutions
Download Application Note 10418: Vincinal Diketones in Beer Using Valve-and-Loop Headspace Analysis
Table of Contents Gas Chromatography-Mass Spectrometry
Complete Brewing Solutions for Beer Analysis
Automated Discrete Photometry Analysis of Nitrosamines
Applications
Nitrosamines, also called N-nitrosodiaklyamines, comprise a class of Experimental Conditions GC-MS/MS Instrument
compounds with a variety of alkyl moieties. These compounds are toxic TRACE 1310 GC
GC and GC-MS
and highly carcinogenic in humans and animals, with higher doses leading iC Injector Module Split/Splitless Injector
Applications to severe liver damage and internal bleeding. Nitrosamines are produced Injector Temperature 250 °C
in beer brewing operations during the process of drying germinated Injection Mode Splitless
malt. Today’s GC-MS triple quadrupole technology delivers the sensitivity Surge Mode 300 KPa
Ion Chromatography
and selectivity to detect nitrosamines at very low concentrations, even Splitless Time 1.0 min
Applications in complex matrix samples like beer. This example describes a turnkey Analytical Column TG-WAX MS, 30m×0.25mm×0.5μm
GC-MS/MS method for routine detection and quantitation of food-borne Carrier Gas He (99.999% purity)
Liquid Chromatography nitrosamine compounds. Flow Rate 1.0 mL/min, constant flow
Oven Program 45 °C for 3 min,
Applications
25 °C/min to 130 °C,
12 °C/min to 230 °C, 1 min hold
BeerNHop Solutions Transfer Line Temperature 250 °C
RT: 7.87 Total Analysis Time 14.7 min
AA: 6336
SN: 31
RT: 8.55 Total Cycle Time 18.4 min
Peer Reviewed Journal References AA: 32766
SN: 231
TriPlus RSH Autosampler
RT: 9.75 RT: 11.35
AA: 1657812 AA: 23756 Injection Volume 1 μL
SN: 2695 SN: 26
Solvent Dichloromethane
RT: 11.80
AA: 25711
RT: 12.06
Standard Runs 3 replicate of injections each
NDMA NPIP
SN: 10
AA: 22994 Dilution of Standard Mix 1 ppb, 5 ppb, 10 ppb, 25 ppb, 100 ppb, 250 ppb,
NDBA SN: 4
NDEA RT: 12.47 500ppb
AA: 100389
NDPA (ISTD) NDBA SN: 40 Internal Standard NDPA added to each calibration level at 50 ppb
100 NPIP Thermo Scientific™ TSQ™ 8000 Triple Quadrupole GC-MS/MS system
80
Relative Abundance
NPYR
60 NPYR Ionization Mode EI
40 Mass Resolution Setting Normal
20 NMOR
NMOR Source Temperature 220 °C
0
8 9 10 11 12 Scan Mode MRM, retention time-based SRM mode
Time (min)
Download Application Note 10315: High Sensitivity Analysis of Nitrosamines Using GC-MS/MS
Table of Contents
Ion Chromatography
Complete Brewing Solutions
Applications
GC and GC-MS
Applications
Ion Chromatography
Applications
Ion Chromatography for Beer Analysis
Liquid Chromatography
Applications
Automated Discrete Photometry Carbohydrates, Alcohols, Organic Acids, Inorganic Anions, and Cations
Applications
Ion chromatography is an efficient technique for the quantification Conditions for Figure 10
of ions in solution. The compounds of interest for the beer industry Column: Dionex CarboPac PA1
GC and GC-MS
range from inorganic ions, organic acids, and hop bittering principles Eluent 1: Deionized water
Applications that contribute to the overall taste and bitterness of the beverage to Eluent 2: 500 mM Sodium hydroxide
proteins, carbohydrates, and alcohols that are monitored to determine Gradient: Time E1 E2 Comments
the extent of fermentation. The finished beer product may be analyzed Initial 99 1 Reequilibrate
Ion Chromatography 5.00 99 1 Inject
to determine the concentration of added preservatives and colorants,
6.00 99 1 Back to Load
Applications in addition to ensuring manufacturing authenticity. The examples below 20.00 91 9
show the separation of malto-oligosaccharides in beer (Figure 9) and the 45.00 0 100
separation of fermentable sugars in wort (Figure 10) by ion exchange 50.00 0 100
Liquid Chromatography
chromatography. Ion-exchange or ion-exclusion chromatography can be Flow Rate: 1.0 mL/min
Applications used for the determination of many classes of compounds of interest to Inj. Volume: 10 µL
the brewing industry, including carbohydrates, alcohols, organic acids, Detection: Pulsed amperometry, gold electrode
0 5 10 15 20 25
Minutes 0 5 10 15 20 25 30
11435 Minutes 11433
Figure 9. Separation of malto-oligosaccharides in an American beer by ion-exchange Figure 10. Separation of fermentable sugars in wort by ion-exchange chromatography
chromatography with pulsed amperometric detection. with pulsed amperometric detection.
Download Application Note 46: Ion Chromatography: A Versatile Technique for the Analysis of Beer
Column: Dionex IonPac AS11-HC-4µm with guard, 4 × 250 mm
Eluent Source: Dionex EGC 500 KOH cartridge
Table of Contents
Ion Chromatography for Beer Analysis
Gradient: 1 mM KOH (-5–8 min), 1–15 mM KOH (8–18 min),
15–30 mM KOH (18–28 min), 30–60 mM KOH (28–38 min),
60 mM KOH (38–45 min)
Complete Brewing Solutions Flow Rate: 1.5 mL/min
Inj. Volume: 10 µL
Temperature: 30 °C
Automated Discrete Photometry Detection:
Determination of Organic Acids
Suppressed conductivity, Dionex ASRS 300 suppressor, 4 mm,
AutoSuppression, recycle mode
Beer Samples: A: Lager, B: Lager 2, C: Light Lager
Applications Sample Prep: Degas, 5-fold dilution
Organic acids are end products of yeast fermentation critical to the flavor
Peaks:
of beer, but are also products of bacterial fermentation that introduce a 1. Quinate 9. Sulfate
GC and GC-MS 2. Fluoride 10. Oxalate
sour flavor, either purposely or unintentionally due to spoilage. The 3. Lactate 11. Fumarate
Applications 4 µm resin particle Dionex IonPac AS11-HC-4 µm anion-exchange 4. Acetate 12. Phosphate
5. Pyruvate 13. Citrate
column is a high resolution, high-capacity column optimized for organic 6. Chloride 14. Isocitrate
7. Succinate + Malate 15. cis-Aconitate
acids in complex matrices, ideal for analysis of beer samples. At standard 8. Carbonate 16. trans-Aconitate
Ion Chromatography
flow rates, this 4 µm resin particle column operates above 3000 psi,
10 6
Applications which necessitates the use of a high-pressure-capable system such
9 12
as the Dionex ICS-5000+ HPIC system. if the advantages of eluent
Liquid Chromatography generation are to be realized. In the example below, three U.S. domestic
lager beers were analyzed on a Dionex HPIC system, using a
Applications 4 µm particle-size column. 7
13
5 8
23 10
4 14
C 1 11
0
BeerNHop Solutions 9
12
7 13
µS 2 8 10
Peer Reviewed Journal References 34 5 14
B 11
Column: Dionex IonPac AS11-HC-4µm with guard, 4 × 250 mm
12 13
9
Eluent Source: Dionex EGC 500 KOH cartridge 7 10
8
Gradient: 1 mM KOH (-5–8 min), 1–15 mM KOH (8–18 min) 12 14
3 5 11 15 16
15–30 mM KOH (18–28 min), 30–60 mM KOH (28–38 min), 4
A
60 mM KOH (38–45 min)
Flow Rate: 1.5 mL/min -20
Injection Volume: 10 µL 0 10 20 30 40 45
Minutes
Temperature: 30 °C
Figure 11. Analysis of beer samples.
Detection: Thermo Scientific™ Dionex™ ASRS™ 300 suppressor, 4mm,
AutoSuppression, recycle mode
Beer Samples: A: Lager, B: Lager 2, C: Light Lager
Sample Prep: Degas, 5-fold dilution
Download Technical Note 126: Determination of Organic Acids in Beer Samples Using an HPIC System
Table of Contents
Ion Chromatography for Beer Analysis
Complete Brewing Solutions
µS 1
4
3 6
2
5
0
0 10 20 30 40
Minutes
Download Application Note 182: Determination of Biogenic Amines in Alcoholic Beverages by Ion Chromatography with
Suppressed Conductivity Detection and Integrated Pulsed Amperometric Detection
Table of Contents
Ion Chromatography for Beer Analysis
Complete Brewing Solutions
µS
µS
6
4
1 4 5 2 3
D
C
B
A 3
-0.5 –1
0 2 4 6 8 10 12 0 10 20
Minutes Minutes
28819
Caption: Figure 13. Municipal drinking water samples. Caption: Figure 14. Cations in municipal drinking water on
the Dionex IonPac CS12A capillary column.
Download Application Update 196: Anion Determinations in Municipal Drinking Water Samples Using EPA Method 300.1 (A) on an
Integrated IC System
Download Application Brief 133: Cost-Effective Determination of Inorganic Anions and Cations in Municipal Drinking Water Using
Capillary Ion Chromatography
Table of Contents
Liquid Chromatography
Complete Brewing Solutions
Automated Discrete Photometry Redefining HPLC and UHPLC to Give You More
Applications
The Thermo Scientific liquid chromatography platform is the most
complete LC solution provided by a single chromatography powerhouse.
GC and GC-MS
By enabling all our Thermo Scientific™ Dionex™ UltiMate™ 3000 systems
Applications to be UHPLC compatible by design, we provide the market-leading
system solution to all users, all laboratories, and all analytes.
We also provide the latest in state-of-the-art UHPLC with the Thermo
Ion Chromatography
Scientific™ Vanquish™ UHPLC system, delivering better separations,
Applications more results, and easier interaction than ever before.
Liquid Chromatography
Our advanced workflow automation and software solutions boost
productivity and ease-of-use of your liquid chromatography systems
Applications beyond traditional concepts:
Applications
GC and GC-MS
Applications
Ion Chromatography
Applications
Liquid Chromatography for Beer Analysis
Liquid Chromatography
Applications
of beer during storage with the formation of haze (e.g., catechins and
EC Parameters: 16 channel array from 0 to +900 mV in +60 mV increments
Applications their polymers the proanthocyanidins). In the chromatograms below,
Column: Thermo Scientific™ Acclaim™ 120,
two beer samples with differing amounts of hops were measured for a C18 (3.0 × 150 mm, 3 µm particle size)
Liquid Chromatography targeted analysis of polyphenols. This example presents multi-channel Flow Rate: 0.65 mL/min
electrochemical array chromatograms for two different beer samples. Injection Volume: 10 or 20 µL
Applications Electrochemical array detection reveals the presence of many more
Mobile Phase A: 20 mM monobasic sodium phosphate, 3% acetonitrile, 0.2%
polyphenols in a high hops beer (Figure 15A) compared to a regular tetrahydrofuran, pH 3.35
BeerNHop Solutions domestic beer (15B). Mobile Phase B: 20 mM monobasic sodium phosphate, 50% acetonitrile,
10% tetrahydrofuran, pH 3.45
Mobile Phase C: 90% methanol
Peer Reviewed Journal References
A
A Gradient: 0-2 min: 2%B/3%C, 30 min: 97%B/3%C, Curve 7 (concave),
2.50
45 min: 97%B/3%C
2.00
1.50
1.00
0.50
0.00
A
B
2.50
2.00
1.50
1.00
0.50
0.00
0.00 10.00 20.00 30.00 40.00 min
Figure 15. Polyphenol method chromatograms of (A) high-hops beer and B) regular
domestic beer.
Download Application Note 1020: Chalconoids and Bitter Acids in Beer by HPLC with UV and Electrochemical Detection
Table of Contents
Liquid Chromatography for Beer Analysis
Complete Brewing Solutions
cis iso 3
A
trans-iso-humulone
adhumulone/humulone
5.0
trans-iso-adhumulone
trans-iso-cohumulone
prenylnaringinen
isoxanthohumol
Peer Reviewed Journal References 4.0
xanthohumol
cohumulone
adlupulone &
cis iso 2
colupulone
cis-iso 1
3.0
lupulone
2.0
1.0
0.0
0.00 10.00 20.00 30.00 40.00 min
A B
trans-iso-humulone
trans-iso-adhumulone
isoxanthohumol
cis iso 2
5.0
adhumulone &
trans-iso-cohumulone
humulone
cohumulone
4.0
prenylnaringinen
xanthohumol
3.0
cis iso 3
2.0
1.0
0.0
0.00 10.00 20.00 30.00 40.00 min
Figure 16. Chromatograms of (A) bitter acids standard mixture; (B) Ultra IPA beer sample.
Download Application Note 1020: Chalconoids and Bitter Acids in Beer by HPLC with UV and Electrochemical Detection
Table of Contents
Liquid Chromatography for Beer Analysis
Complete Brewing Solutions
Applications an Irish stout, and an American high-hops ultra IPA. Metabolomic Irish
Beer 6
Stout
profiles containing several hundred analytes—including both known
Liquid Chromatography and unknown compounds—were measured in each sample. Principal
Belgian Beer 11
component analysis (PCA) was then used to differentiate samples for both
Applications EC data (Figure 17, Plot A) and UV data (Figure 17, Plot B).
B Factor2
BEER 10
Bavarian, BEER 13
Belgian Microbrews,
Ultra IPA
BEER 6
BEER 7
Figure 17. Principal component plots for (A) EC and (B) UV data
(USA = American).
Download Application Note 1065: Gradient HPLC Method for Analysis of Beer Polyphenols, Proanthocyanidins,
and Bitter Acids Using a Novel Spectro-Electro Array Platform
Table of Contents
Liquid Chromatography for Beer Analysis
Complete Brewing Solutions
1
2
5 6
0 3 6 9
Minutes
Download Application Brief 153: Save the Flavor—Robust Iso-α-Acids Assaying in Beer within Ten Minutes
Table of Contents
Liquid Chromatography for Beer Analysis
Complete Brewing Solutions
10. Colupulone
11. n-Lupulone
12. Adlupulone
2
9 10
3
11
1 6
12
5
0 5 10 15
Minutes
Figure 19. Chromatogram of hop bitter acids and iso-α-acids in beer.
Download Application Brief 155: Monitor the Brewing Process with LC—Transformation of Hop α-Acids into Beer Iso-α-Acids
Table of Contents
Liquid Chromatography for Beer Analysis
Complete Brewing Solutions
12. Tetrahydro-cis-Isoadhumulone
4
9
12
2 7
3
1 6
5 11
0 5 10 15
Minutes
Figure 20. Chromatogram of iso-α-acids and tetrahydro-iso-α-acids in beer.
Download Application Brief 156: The Everlasting Paradigm—Keep Beer Tradition or Prevent Beer from a Skunky Off-Flavor?
Table of Contents
BeerNHop Solutions
Complete Brewing Solutions
Absorbance
method, provides no information about the individual contents of each 1 3
iso-α-acid variant. Furthermore, non-bitter components are also detected
6
5
and adulterate that BU value. Hence, only the measurement of the pure 2000 mAU 40 mAU 4 13 min
Ion Chromatography Beer matrix
2 Retention Time
Absorbance
concentration of iso-α-acids offers precise and comparable information The BeerNHop Solution for Occasional 3 Beer Analyses
Applications 1
6
about genuine beer bitterness. 2000 mAU 40 mAU 4 5
4
2 13 min
Beer matrix 40 mAU
Absorbance
Absorbance
2
Reduced beer matrix Retention Time
Liquid Chromatography High performance liquid chromatography (HPLC) is the only analytical +
solvent peak 1
1
3
53
6
Scientific BeerNHops Solutions for isohumulones in beer provide specific 40 mAU Retention
Retention Time
Time
Absorbance
2
Reduced beer matrix
determination and quantitation of all major cis- and trans-isomer variants +
solvent peak
1 3
BeerNHop Solutions of the iso-α-acids present in beer. 40 mAU
4
5
6
15 min
Absorbance
70 mAU 4 2 Legend (all chromatograms)
Reduced beer matrix Retention Time 1 - Trans-iso-cohumulone
Absorbance
2 Unknowns
Solventsolvent
peak peak
+
2 - Cis-iso-cohumulone
Peer Reviewed Journal References Each Complete Solution for Isohumulones in Beer consists of an UltiMate The BeerNHop Solution for Direct 3
Injection
1
of
3
Untreated
5
6 Beer Samples 3 - Trans-iso-n-humulone
4 - Cis-iso-n-humulone
15 min
1 5 - Trans-iso-adhumulone
3000 system package and its related isohumulones starter kit. Both 4
5
6
Retention Time 9 min
Legend (all chromatograms)
6 - Cis-iso-adhumulone
Absorbance
2 2 - Cis-iso-cohumulone
Solvent peak
software, as well as the UltiMate 3000 hardware parts needed to set 3
3 - Trans-iso-n-humulone
4 - Cis-iso-n-humulone
1 5 - Trans-iso-adhumulone
up the entire UHPLC -focused system. Add the appropriate Thermo
+ 70 mAU 4
5
6
9 min
Legend (all chromatograms)
6 - Cis-iso-adhumulone
1 - Trans-iso-cohumulone
Absorbance
2 Unknowns 2 - Cis-iso-cohumulone
Scientific Isohumulones Starter Kit, providing all of the necessary Solvent peak
Retention Time 3 - Trans-iso-n-humulone
4 - Cis-iso-n-humulone
consumables, and the analysis of isohumulones in beer can be 1
3
5
6
5 - Trans-iso-adhumulone
6 - Cis-iso-adhumulone
9 min
started immediately. Retention Time
Recommended Resources
Complete Brewing Solutions
Applications
Identification and determination of phenolic constituents in natural beverages and plant Achilli, G.; Cellerino, G.; Gamache, P.; J. Chromatogr., 632, 111- 1993
GC and GC-MS extracts by means of a coulometric electrode array system. Melzi d’Eril, G. 117.
HPLC mit coulometrischer elektroden-array-detektion: Bestimmung von 1-O-trans-p- Bernwieser, I.; Patzold, R.; Galensa, R.; Z. Lebensm Unters Forsch, 1994
Applications cumaraoylglycerol in sorghumhirse, mais und bier. Sontag, G. 198, 40-43.
Determination of Free Phenolic Acids in Wort and Beer by Coulometric Array Detection. Floridi, S.; Montanari, L.; Marconi, O; J. Agric. Food Chem., 51, 2003
Fantozzi, P. 1548-1554.
Ion Chromatography
Applications RP-HPLC analysis of phenolic compounds and flavonoids in beverages and plant extracts Jandera. P.; Skeifíková, V.; Rehová, L.; J. Sep. Sci., 28, 1005-1022. 2005
using a CoulArray detector. Hájek, T.; Baldriánová, L.; Skopová, G.;
Kellner, V.; Horna, A.
Liquid Chromatography Sensitive electrochemical detection method for alpha-acids, beta-acids and xanthohumol in Kac, J.; Vovk, T. J. Chromatogr., B: Anal. 2007
hops (Humulus lupulus L.). Technol. Biomed. Life Sci. 850
Applications (1–2), 531–537.
Determination of proanthocyanidins and catechins in beer and barley by high-performance Madigan, D.; McMurrough, I; Smyth, M. Analyst, 119, 863-867. 1994
liquid chromatography with dual-electrode electrochemical detection.
BeerNHop Solutions Phenolic acids from beer are absorbed and extensively metabolized in humans. Nardini, M.; Natella, F.; Scaccini, C.; J. Nutr. Biochem. 17 (1), 2006
Ghiselli, A. 14–22.
Optimization of gradient HPLC analysis of phenolic compounds and flavonoids in beer using a Rehová, L.; Skeríková, V.; Jandera, P. J. Sep. Sci., 27, 1345-1359. 2004
Peer Reviewed Journal References
CoulArray detector.
Fast Determination of Iso-alpha-Acids in Untreated Beer Samples with Specific Separation Heidorn, M. J. Am. Soc. Brew. Chem. 2013
into cis- and trans-Isomers.
www.thermofisher.com/chromatography
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