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1. AOAC. Official Method 999.07 (2000). Aflatoxins and total aflatoxins in peanut butter, pistachio paste, fig paste
and paprika powder. Immunoaffinity column-liquid chromatography with post-column derivatization. First action
1999. J. AOAC Int. 83: 320.
2. Bryden, W. L. 1994. Neuromycotoxicoses in Australia. PP. 363-368. In: S. M. Colegate and P. R. Dorling (Eds.),
Plant-Associated Toxins. CAB International, Wallingford, UK.
3. Charmley, L. L., H. L. Trenholm and D. B. Prelusky. 1995. Mycotoxins: their origin, impact and importance; insight
into common methods of control and elimination. PP. 41-63 In: T. Lyons and K. A. Jacques (Eds.),

www.SID.ir
... ; 3:/ 6 7 "3 48 4 49 B1 + , -. / + 0 1 2 3 " 45"

Biotechnology in the feed industry. Proceedings of alltech's 11th annual symposium.

4. Cole, R. J. 1986. Etiology of Turkey-"X" disease in retrospect: a case for involvement of cyclopiazonic acid.
Mycotoxin Res. 2: 3-7.
5. Coles, E. H.1974. Veterinary Clinical Pathology. 2nd ed., W. B. Sauders Co., London.
6. Dafalla, R., A. I. Yagi and S. E. I. Adam. 1987. Experimental aflatoxicosis in Hybro-type chicks: sequential changes
in growth and serum constituents and histopathological changes. Vet. Hum. Toxicol. 29: 222-226.
7. Darman Kave Research Laboratory. 2001. Isfahan, Iran.
8. Dersjant-Li, Y., M. W. A. Verstegen and W. J. J. Gerrits. 2003. The impact of low concentrations of aflatoxin,
deoxynivalenol of fumonisin in diets on growing pigs and poultry. Nutr. Res. Rev. 16: 223-239.
9. Devlin, T. M. 2002. Textbook of Biochemistry. Wiley-Liss, New York, USA.
10. Edrington, T. S., L. F. Kubena, R. B. Harvey and G. E. Rottinghaus. 1997. Enfluence of a superactivated charcoal
on the toxic effects of aflatoxin or t-2 toxin in growing broilers. Poult. Sci. 76:1205–1211.
11. Giroir, L. E., W. E. Huff, L. F. Kubena, R. B. Harvey, M. H. Elissalde, D. A. Witzel, A. G. Yersin and G. W. Ivie.
1991. The individual and combined toxicity of kojic acid and aflatoxin in broiler chickens. Poult. Sci. 70: 1351-
1356.
12. Huff, W. E., L. F. Kubena, R. B. Harvey, D. E. Corrier and H. H. Mollenhauer. 1986. Progression of aflatoxicosis in
broiler chickens. Poult. Sci. 65: 1891-1899.

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13. Hussein, H. S. and J. M. Brasel, 2001. Toxicity, metabolism and impact of mycotoxins on humans and animals.
Toxicol. 167: 101-134.
14. Kubena, L. F., R. B. Harvey, W. E. Huff, D. E. Corrier, T. D. Phillips and G. E. Rottinghaus. 1990. Efficacy of a

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hydrated sodium calcium aluminosilicate to reduce the toxicity of aflatoxin and T-2 toxin.. Poult. Sci. 69:1078-
1086.
15. Kubena, L. F., R. B. Harvey, W. E. Huff, M. H. Elissalde, A. G. Yersin, T. D. Phillips and G. E. Rottinghaus, 1993.
Efficacy of a hydrated sodium calcium aluminosilicate to reduce the toxicity of aflatoxin and diacetoxyscirpenol.
Poult. Sci. 72:51–59.
16. Ledoux, D. R., G. E. Rottinghaus, A. J. Bermudez and M. Alonso-Debolt. 1998. Efficacy of a hydrated sodium
of
calcium aluminosilicate to ameliorate the toxic effects of aflatoxin in broiler chicks. Poult. Sci. 77:204–210.
17. Leeson, S., G. Diaz and J. D. Summers. 1995. Poultry metabolic disorders and mycotoxins. University books,
Guelph, Ontario, Canada.
18. National Research Council. 1994. Nutrient Requirement of Poultry. 8th Revised ed., National academy press,
Washington, DC.
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19. Quist, C. F., D. I. Bounous, J. V. Kilburn, V. F. Nettles and R. D. Wyatt. 2000. The effect of dietary aflatoxin on
wild turkey poults. J. Wildlife Dis. 36: 436-444.
20. Rosa, C .A. R., R. Miazzo, C. Magnoli, M. Salvano, S. M. Chiacchiera, S. Ferrero, M. Saenz, E.C.Q. Carvalho and
A. Dalcero, 2001. Evaluation of the efficacy of bentonite from the south of argentina to ameliorate the toxic
effects of aflatoxin in broilers. Poult. Sci. 80:139–144.
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21. SAS institute. 1985. SAS Users Guide Statics. Version 5 ed., SAS institute Inc., Cary, NC.
22. Shotwell, O. L., C. W. Hesseltine, R. D. Stubblefield and W. G. Sorenson. 1966. Production of aflatoxin on rice .
Appl. Microbiol. 14: 425-428.
23. Tedesco, D., S. Steidler, S. Galletti, M. Tameni, O. Sanzogni and L. Ravarotto. 2004. Efficacy of silymarine-
phospholipid complex in reducing the toxicity of aflatoxin B1 in broiler chicks. Poult. Sci. 83: 1839-1843.
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