TM

PERFORMANCE MATERIALS

Reagents for Mindray analyzers

Mindray BC-5500, BC-5200
tWBC + 5 part differential analysis
Reagent Description Product Nr. Pack size
Diluent Diluid M5 3419.9020PC 20L
Lysing reagent CyMet MH CN Free 3497.0500PE 500 mL
Baso reagent CyMet MBA 3489.1000PE 1L
Diff reagent CyMet MD(I) 3418.1000PE 1L
Diff reagent CyMet MD(II) 3488.0500PE 500 ml
Cleaner ProClean MX5 3498.1000PE 1L
Hematology Control BC Diff 5 Control L/N/H 3613, 3614, 3615 3.0 ml
The connection of the 20L polycube can be optimised by using our adaptor (code 82166).

Mindray BC-5300
tWBC + 5 part differential analysis
Application Description Product Nr. Pack size
Diluent Diluid M5 3419.9020PC 20 L
Lysing reagent CyMet MD(I) 53 2984.1000PE 1L
CyMet MD(II) 3488.0500PE 500 ml
CyMet LH 53 2985.1000PE 1L
Cleaner Proclean MX5 3498.1000PE 5L
Hematology Control BC Diff 5 Control L/N/H 3613/3614/3615 3.0 ml

Mindray BC-2800, BC-3000 Plus, BC-3200, Automated

tWBC + 3 part differential analysis
Application Description Product Nr. Pack size
Diluent Diluid Mindray 3439.9020PC 20 L
Lysing reagent CyMet Mindray 3441.0500PE 500 ml
CyMet Mindray CN Free 3440.0500PE 500 ml
Rinsing/Auxiliaries Rinse Mindray 3442.5000PE 5L
Emergency Cleaning ProClean Plus 3901 100 ml
Hypochlorite 0.5% 3917 1L
3917.5000 5L
Hematology Control 8 Parameter Control L/N/H 3427/3428/3429 2.5 ml
3463/3464/3465 2.5 ml, pierceable
3701/3702/3703 4.5 ml
Hematology Control 3 Diff Control L/N/H 3433/3434/3435 2.5 ml
3820/3821/3822 4.5 ml

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PERFORMANCE MATERIALS

melet schloesing
Mindray BC-2000, BC-2300, Semi-Automated
tWBC + 3 part differential analysis
Application Description Product Nr. Pack size
Diluent Diluid Mindray 3439.9020PC 20 L
Lysing reagent Lyzerglobin PCE 3771 10 x 10 ml
CyMet Mindray 3441.0500PE 500 ml for BC 2300

mindray
Cleaning ProClean Extra 3862.1000 1L
3862.5000 5L
Emergency Cleaning ProClean Plus 3901 100 ml
Hypochlorite 0.5% 3917 1L
3917.5000 5L

nihon kohden
Hematology Control 8 Parameter Control L/N/H 3427/3428/3429 2.5 ml
3463/3464/3465 2.5 ml, pierceable
3701/3702/3703 4.5 ml
Hematology Control 3 Diff Control L/N/H 3433/3434/3435 2.5 ml
3820/3821/3822 4.5 ml

Diluid, CyMet and ProClean are all trademarks of Avantor Performance Materials Inc.

orphee
Cross list: Mindray reagents vs. J.T.Baker
Mindray BC-3000 H Nr. J.T.Baker JTB Nr.
Lyse A12-000044 500 ml CyMet Mindray 3441.0500PE

seac
CyMet Mindray CN Free 3440.0500PE
Diluent A12-000042 5.5L Diluid Mindray 3439.9020PC
Diluent A12-000047 20L Diluid Mindray 3439.9020PC
Rinse A12-000043 5.5L Rinse Mindray 3442.5000PE
Rinse A12-000048 20L Rinse Mindray 3442.5000PE

swelab
E-Z Cleaner A12-000045100 ml ProClean Plus 3901
Probe cleanser A12-00004617 ml Hypochlorite 3917

Mindray BC-5500/5200 H. Nr J.T.Baker JTB Nr.

M-50D Diluent A12-000167 Diluid M5 3419.9020PC

sysmex
M-50 LHLysing reagent A12-000221 CyMet MH CN Free 3497.0500PE
M-50 LBA Baso reagent A12-000224 CyMet MBA 3489.1000PE
M-50 LEO (I) Diff reagent A12-000219 CyMet MD(I) 3418.1000PE
M-50 LEO (II) Diff reagent A12-000220 CyMet MD(II) 3488.0500PE
M-50 Cleanser A12-000225 ProClean MX5 3498.1000PE

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controls
cleaners
pbs
Stains

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PERFORMANCE MATERIALS

Priming and Calibration of Mindray Analyzers

Mindray BC-3000 Plus
Before switching from original reagents to Avantor Performance
Materials reagents it is important to know that the instrument is
running without any problems and that the values measured for
the blood controls (all three levels) are within the specifications
of the assay value sheet. Therefore it is necessary to run blood
controls or some fresh reference patient samples before you
switch to our reagents. These values are necessary for any
calibration afterwards.
Priming of Mindray BC-3000plus
1. Choose the option <SERVICE> from the main menu of the
software program by using the cursor keys. Then choose
<MAINTENANCE> and press <ENTER>.
Remove the aspirating tube from the Mindray reagent
cubitainer which you want to replace. Don’t lay the tubes
on tissues or dusty surfaces. Place them in clean beakers.
Reagent alarms will occur!
It is important to rinse the outside surface of the aspirating
tubes with distilled or de-ionised water to prevent any kind
of reagent contamination.
2. Choose the functional key <PRIME> followed by <ENTER>
from the reagent you want to replace:
Mindray Avantor
M-30D Diluent = Diluid Mindray
M-30R Rinse = Rinse Mindray
M-30L Lyse = CyMet Mindray or CyMet Mindray
CN Free
For example press <DILUENT PRIME>, <ENTER> when you
want to replace the M-30D Diluent for Diluid Mindray.
Hold the reagent sensor in a way (move sensor up) that the
reagent alarm will stop and that you can activate a reagent
prime with air.
3. Place the aspiration tube in the cubitainer with Avantor
reagent. Choose the functional key <PRIME> followed by
<ENTER> from the reagent you want to replace:
INFORMATION IN THIS DISTRIBUTOR CATALOGUE. ALSO REFER
TO SECTION 5 OF THE BC-3000PLUS OPERATOR’S MANUAL.
1. Run blood controls or the same fresh patient samples that
you ran before changing the original reagents for Avantor
reagents.
2. Choose the option <CALIBRATION> from the MAIN menu.
Then choose <MANUAL CALIBRATION> and press <ENTER>
to access Manual Calibration screen. The calibration factors
are shown in a table.
3. Calculate the New Calibration Factors. Use the following
formula to calculate the new calibration factors:
old factor x reference value
New factor = ---------------------------------------------
Average of test value
See EXAMPLE at the bottom of the page.
4. Enter the new Calibration Factors for all the parameters you
want to calibrate.
5. Confirm the new Calibration Factors.
6. After entering new factors, run the controls or patient
samples again. Verify the results.
Example
• 5 fresh patient samples were measured with Mindray
reagents. Next MCV values are measured: 91, 88, 89, 87, 89.
The mean value for MCV is 89.
• After priming the Avantor reagents into the instrument the
same 5 fresh patient samples gave next MCV values: 86, 84,
87, 84, 86. The mean value of MCV is 86.
• Old calibration factor in the whole blood mode is 97,2 %.
old factor x reference value
• New factor = -----------------------------------------------------
Average of test value
97,2 x 89
= ------------------ = 100,6 %
86

REPEAT STEPS 1 TO 3 FOR ALL THE THREE REAGENTS

CONNECTED!! Note: For Automatic Calibration please refer to Section 5.4 in
the BC-3000plus Operator’s Manual.
4. Choose the functional key <EMPTY BATHS>, <ENTER> to
empty the diluent from the WBC and RBC baths. After that,
press <ENTER> to prime the Diluent into the baths

5. Perform at least 5 blank counts and check the background

values. Acceptable values for the background are:
WBC < 0.5 K/µl HGB < 0.2 g/dL
RBC < 0.05 M/µl PLT < 10 K/µl
Repeat background counts until acceptable values are
found.

Manual calibration of Mindray BC-3000Plus Analyzers

FOR CALIBRATION INSTRUCTIONS AND CALCULATING NEW
CALIBRATION FACTORS REFER TO THE PARAGRAPH GENERAL

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PERFORMANCE MATERIALS

melet schloesing
Mindray BC-5500
• T he separation between Monocytes and Neutrophils
1. Important NOTES (see figure 2) is improved when using the JT Baker
reagents in comparison to the Mindray reagents. The
Please read these notes prior to installation of the

J.T.Baker reagent package on the Mindray BC5500 Auto
Hematology analyzer.
• T he recipe for the CyMet MD(I) has been improved on
mindray
Monocytes are positioned closer to the Lymphocyte
area. Mindray reagents tend to give more often
Monocytosis flagging, where JT baker reagents tend to
give more often Abn./Atypical Lym? The Lymphocyte
cluster is more stretched with JT Baker reagents, but the

nihon kohden
Eosinophilia detection. Therefore these reagent total amount counted is equal.
replacement instructions are intended for use with
CyMet MD(I) lots higher than lot 0928900030 and all lots
of CyMet MBA

• This current reagent system has been tested on BC5500

with software version up to 1.13. In case of any newer

orphee
software versions or any hardware modification of the
BC5500 model we cannot guarantee the performance of
the reagent.
J.T. BAKER MINDRAY
• Reagents for the Mindray BC5500 are bottled in similar Figure 2. Improved Monocytes / Neutrophils separation
square bottles as the original reagents. For the Diluid J.T. Baker vs. Mindray
cubitainer we will provide our customers a special

adapter to overcome the difference in height due to the
fixed Diluent reagent aspiration tube.
• T o guarantee optimal performance of the JT Baker
reagents, the priming protocol mentioned in this
seac
Therefore we advise to set the Sensitivity for this

Abn./Atypical Lym flag to 4 instead of 5. Go to
SETUP, Advanced, Flag Sensitivity, Edit, Abn./
Atypical Lym and change the value to 4.

handout should be followed every time you switch from
original Mindray reagents to JT Baker reagents or vice
versa. Especially the priming protocol for the Diluent and
the CyMet MD(I) reagents should be performed correctly
to avoid cross contamination between the reagents.
swelab
• J T Baker CyMet MBA reagent causes an effect on the
position of the mononuclear and polynuclear white
blood cells and Basophil population in the BASO-
scattergraph (see figure 3). The Mindray Basophil reagent
is much stronger than the JT Baker Basophil reagent
resulting in different gain settings for both reagent

• Before changing any reagents on the Mindray BC5500
instrument, run at least 5-10 fresh human samples or
BC-Diff 5 control blood with the original Mindray
reagents for reference and calibration purposes.
sysmex
packages. Please follow the Optical System Setup
procedure for Basophils mentioned in this handout.
Figure 3. Basophil subpopulation J.T. Baker vs. Mindray

• J .T. Baker differential reagents result in a different

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separation between the mononuclear (Lymphocytes
and Monocytes) and polynuclear (Neutrophils,
Eosinophils and Basophils) cells compared to Mindray
reagents. Figure 1 shows and example of the different
mononuclear and polynuclear white blood cells
controls

2. Priming protocol

Use next cross list for correct reagent installation and usage.
cleaners

MINDRAY J.T. BAKER

M-50D Diluent Diluid M5
M-50LEO(I) Lyse CyMet MD(I)
M-50LEO(II) Lyse CyMet MD(II)
M-50LH Lyse CyMet MH CN Free
M-50LBA Lyse CyMet MBA
pbs

Figure 1. White blood cells subpopulations M-50 Cleanser Proclean MX5

4-part Diff Scattergraph M-50P Probe Cleanser Hypochlorite 0.5%
Stains

67
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PERFORMANCE MATERIALS

For the complete Deprime of the instrument we advise to MAS = High angle scatter (horizontal X-axis), related to
use the Pack-up function from the Overall maintenance complexity
screen. This is the best method to flush out the original LAS = Low angle scatter (vertical Y-axis), related to size
reagents, flush the system with water and prime the system
with JT Baker reagents. 7µm standard particle test (DIFF & BASO channel):
:
Pack-up protocol (see page 10-33 operator manual)
Click the “service” icon in the “Main” screen and click the 1) In the count screen select “Mode” and change to the
“Overall” button to enter the maintenance screen. Proceed as “OV-PD” mode and click “Diluent” to dispense diluent
follows: into an empty tube 3 times.
1. Click the “Pack-up” button at the “Overall” screen.
2. Click “Yes” to proceed with the pack-up. 2) Dispense 1 drop of 7µm standard particle into the
3. Remove all reagent pickup tube assemblies from their centrifugal tube and mix them.
bottles and containers when prompted on the screen.
4. Click “OK” to start the fluidic system emptying. 3) Change the mode to “OV-Whole blood-CBC+5DIFF” and
5. A progress bar pops up and shows the remaining time. test the dilution as whole blood
6. When prompted place all reagent pickup assemblies
into a container with distilled water. 4) Then go to the “Review > OpAdjust” screen and select
7. Click “OK” to start to flush the analyzer with distilled the “DIFF” channel (see figure 5).
water.
8. After this flushing process a dialog box will ask to
remove all reagent pickup assemblies from the distilled
water.
9. Remove them and place them in a clean beaker.
10. Click “OK” to start to empty the fluidic system.
11. The instrument will power off automatically.
12. Power on the instrument and wait for initialization to
finish.
13. Several alarms will sound.
14. Click the “Count” button and click on the upper alarm
box.
15. Place all reagent pickup assemblies in their correspon-
ding J.T. baker reagents.
16. Click on the “Remove error” button and reagent priming
will start for all the reagents.
17. When ready, perform at least 5 background counts. Figure 4. Optical Adjust screen (OpAdjust)5)
18. Perform the Optical System setup protocol.
19. After that perform the calibration of the instrument. 5) Click “Calculate” to check the four parameters:
Mindray reagents: AS-peak, 88 ± 4; LAS-peak, 45 ± 4
JT Baker reagents: MAS-peak, 88 ± 4; LAS-peak, 45 ± 4
3. Optical System Setup
MAS 0.1max width, =14
Next methods should be used setting up the new types of LAS 0.1max width, =14
Optical systems of the BC5500 instruments with JT Baker
reagents correctly: 6) Select the “BASO” channel and click “Calculate” to check
1. Using standard latex particles of 7µm. the two parameters:
2. Using fresh human samples. Mindray reagents: MAS-peak, 147 ± 4; LAS-peak, 58 ± 4
Both methods are perfectly described in Mindray instruction JT Baker reagents: MAS-peak, 89 ± 4; LAS-peak, 45 ± 4
materials.
7) If the MAS/LAS-peak value is out of normal range, input
3.1. I nstruction on using the standard particles of 7µm . the target value and press “>>>” to calculate the new
Gain. Please write down the new Gain; then access
For the new optical system, 7µm standard particle is used for “Setup > Gain” screen to modify the gains accordingly.
both channels (DIFF & BASO).

For the DIFF channel there are no differences for the

MAS-peak and LAS-peak between the Mindray and JT
Baker reagents.
For the BASO channel there are differences between the
Mindray and JT Baker reagents. MAS-peaks and
LAS-peaks are different.

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PERFORMANCE MATERIALS

3.2. Instructions using fresh samples
IIt is advised to setup the instrument using standard
latex particles. are not available, gains can be adjusted for
the DIFF and BASO channels according to experience.
Experienced customers can used next quick setup method
to adjust the optical gains for DIFF and BASO channels.
melet schloesing
There are several important checks to see if the MAS-peak and
LAS-peak gains for the BASO are set up correctly:
• There should be a clear separation between the Basophils
and the rest of the white blood cells.
• If the bottom of the total WBC population is cut by a

mindray
straight line, the total WBC count is not ok and the LAS
Quick Optical Gain Setup method: PEAK gain has to be increased.
• See figure 7 for an incorrect separation between Basophils
WBC LAS DIFF J.T. Baker = WBC LAS DIFF Mindray and other White Blood Cell populations. MAS PEAK gain
WBC MAS DIFF J.T. Baker = WBC MAS DIFF Mindray has to be lowered.

nihon kohden
WBC LAS BASO J.T. Baker = WBC LAS BASO Mindray -71
WBC MAS BASO J.T. Baker = WBC MAS BASO Mindray -66

Example:
Gain Mindray J.T. Baker
WBC LAS DIFF 97 97

orphee
WBC MAS DIFF 70 70
WBC LAS BASO 161 90 (161 - 70)
WBC MAS BASO 191 125 (191 - 66)

After correct Optical Gain Setup a normal scatter graph for Figure 7. Incorrect separation Basophil population
the 4-part Differential with JT Baker reagents is shown in

seac
figure 5.

swelab
sysmex
Figure 5. WBC 4-part Differential

There are several important checks to see if the MAS-peak and

LAS-peak gains for the DIFF are set up correctly:
• There should be a clear separation between Lymphocytes
and Monocytes populations.

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• There should be a clear separation between
Neutrophils+Baso and Eosinophils populations.
• The DIFF scattergraph should be 3/4 or 4/5 of the DIFF
screen (LAS-peak).
• The centre of the “Neu+Baso” should be in the right middle
controls

of DIFF scattergraph. Otherwise there will be too many “left

shift” and “immature cells” flags.

A normal scatter graph for the Basophils with J.T. Baker reagents
is shown in figure 6.
cleaners
pbs

Figure 6. Basophil population

Stains

69
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PERFORMANCE MATERIALS

4. Calibration

It is recommended before changing to JT Baker reagents to

run fresh human samples or the BC-Diff 5 control blood as
reference.

After installation of JT Baker reagents on the Mindray BC5500

instrument calibration is necessary for several CBC parame-
ters. Especially HGB and MCV are affected by the change of
reagents.

Please refer to Chapter 9 of the Operation Manual how to use

the calibration programs. As well you can use the Quick
Calibration guide mentioned below.
.

Quick Calibration guide

If you ran fresh human samples with original Mindray

reagents before changing to JT Baker reagents you can use
next quick calibration guide to perform the calibration.

Step 1: Calculate the mean for parameters WBC, RBC, HGB,

MCV and PLT from the samples that were
measured with original Mindray reagents
(reference values)

Step 2:  easure the same samples, but now with JT Baker

M
reagents

Step 3: Calculate the mean for parameters WBC, RBC, HGB,

MCV and PLT from the samples that were now
measured with JT Baker reagents

Step 4: At the “Main” menu, click the “Calibration” icon to

enter the “Calibration” screen. Print the old
calibration factors

Step 5: Calculate for every parameter the new calibration

factor
For example: MCVmindray = 92, MCVJ.T.Baker = 88 and
the old calibration factor is 99.0

MCVmindray
New Cal factor for MCV = ------------------ * Old factor MCV =
MCVJ.T.Baker


92
New Cal factor for MCV = ---- * 99.0 = 103.5
88

Step 6: Enter the new factors in the Calibration

screen

Step 7: For verification measure the same samples

again and compare the averages with the
reference values

Step 8: If values are still not ok, repeat Step 5 to 7

70
About Avantor™ Performance Materials
Avantor Performance Materials manufactures and markets high-performance chemistries and materials
around the world under several respected brand names, including the J.T.Baker®, Macron Fine Chemicals™,
Rankem™, Diagnova™, BeneSphera™, and POCH™ brands.

Avantor products are used in a wide range of industries. Our biomedical and life science solutions are
used in academic, industry and quality control laboratories for research, pharmaceutical production and
medical lab testing, while our electronics solutions are used in the manufacturing of semiconductors and
flat panel displays. Based in Center Valley, Pennsylvania (USA), Avantor is owned by an affiliate of New
Mountain Capital, LLC.

For additional information please visit www.avantormaterials.com or follow www.twitter.com/avantor_news

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Trademarks are owned by Avantor Performance Materials, Inc. or its affiliates unless otherwise noted.