Professional Documents
Culture Documents
™ Coagulation Systems: Instrumentation Laboratory
™ Coagulation Systems: Instrumentation Laboratory
REVISION STATUS
TRADEMARKS
Instrumentation Laboratory, , ACL TOP®, HemosIL® and AccuTrak® are registered trademarks of
Instrumentation Laboratory company in the Unites States and several international jurisdictions.
All other trademarks, registered trademarks, service marks, products or services are trademarks or registered
trademarks of their respective holders.
BECKMAN COULTER, INC. URGES ITS CUSTOMERS TO COMPLY WITH ALL NATIONAL HEALTH AND
SAFETY STANDARDS SUCH AS THE USE OF BARRIER PROTECTION. THIS MAY INCLUDE, BUT IT IS
NOT LIMITED TO, PROTECTIVE EYEWEAR, GLOVES AND SUITABLE LABORATORY ATTIRE WHEN
OPERATING OR MAINTAINING THIS OR ANY OTHER AUTOMATED LABORATORY ANALYZER.
WARNING
Risk of operator injury if:
• All doors, covers and panels are not closed and secured in place prior to and during instrument
operation.
• The integrity of safety interlocks and sensors is compromised.
• Instrument alarms and error messages are not acknowledged and acted upon.
• You contact moving parts.
• You mishandle broken parts.
• Doors, covers and panels are not opened, closed, removed and/or replaced with care.
• Improper tools are used for troubleshooting.
To avoid injury:
• Keep doors, covers and panels closed and secured in place while the instrument is in use.
• Take full advantage of the safety features of the instrument. Do not defeat safety interlocks and sensors.
• Acknowledge and act upon instrument alarms and error messages.
• Keep away from moving parts.
• Report any broken parts to your Beckman Coulter Representative.
• Open/remove and close/replace doors, covers and panels with care.
• Use the proper tools for troubleshooting.
CAUTION
System integrity might be compromised and operational failures might occur if:
• This equipment is used in a manner other than specified. Operate the instrument as instructed in the
Product Manuals.
• You introduce software that is not authorized by Beckman Coulter into your computer. Only operate
your system’s computer with software authorized by Beckman Coulter.
• You install software that is not an original copyrighted version. Only use software that is an original
copyrighted version to prevent virus contamination.
IMPORTANT
If you purchased this product from anyone other than Beckman Coulter or an authorized Beckman Coulter
distributor, and, if it is not presently under a Beckman Coulter service maintenance agreement, Beckman Coulter
cannot guarantee that the product is fitted with the most current mandatory engineering revisions or that you will
receive the most current information bulletins concerning the product. If you purchased this product from a third
party and would like further information concerning this topic, call your Beckman Coulter Representative.
RUN QC 1
OBJECTIVES
Given:
• The ACL TOP Family coagulation system is performing to specifications
• Controls are reconstituted and ready to run
• The instrument is setup and ready to run PT-RP and APTT-SS
Action:
• Run all QC levels for PT-RP and APTT-SS from the vial
• Review QC Analysis
SKILL CHECK
• Successfully complete all steps in the Performance Guide
• Correctly answer the questions in the Skill Check
MODULE RESOURCES
• ACL TOP Operator’s Manual – Contents Tab ► Quality Control ►
Performing Quality Control
• Performance Guide – Run QC 1
Run QC 1
Performance Guide
• ACL TOP 700 or ACL TOP 700 CTS or ACL TOP 700 LAS
Review QC Analysis
1. Verify that you are on the QC Results List (top left corner). If not, choose
QC ►Results List from the Menu Bar.
2. How do you know which QC levels are running? _______________________
3. Double click anywhere on the QC Results List to display the Material/Tests
Definition Tree on the QC Statistics screen.
4. Single click on the words Normal C. Assayed under PT-RP from the
Material/Tests Definition Tree to display the QC data for this level.
NOTE:
The checkmark before the QC level is only
used to select which QC levels to run.
TIP: Double clicking on the word FAILED from the QC Results List to open
the QC Statistics screen displays the failed QC backlit in gray or blue on the
Material/Tests Definition Tree.
SKILL CHECK
1. What QC runs when you select the check box next to a test? ____________________
________________________________________________________________________
2. What QC runs when you select the check box next to the QC level?
________________________________________________________________________
RUN QC 2
OBJECTIVES
Given:
• The ACL TOP Family coagulation system is performing to specifications
• All controls required for this module have been reconstituted and are ready to
be run
• PT-RP, APTT-SS, Fib-C and DDHS tests are enabled and calibrated
Action:
• Run QC from Vials
• Run QC from sample cups
• Repeat FAILED QC
• Review QC Statistics
• Add a Comment to FAILED QC
SKILL CHECK
• Successfully complete all steps in the Performance Guide
• Correctly answer the questions in the Skill Check
MODULE RESOURCES
• ACL TOP Operator’s Manual
Run QC 2
Performance Guide
1. Load the D-Dimer Low and D-Dimer High controls on the instrument as
follows:
• ACL TOP 500 CTS
• ACL TOP 700 or ACL TOP 700 CTS or ACL TOP 700 LAS
4. Single click or touch Clear Tree Selection to clear all selections from
the last run.
5. Select DDHS from the Navigation Tree.
1. Pour the Normal C. Assayed, High Abn C. Assayed and Low Fib. Control
into labeled 2.0mL sample cups.
8. Single click or touch Add/Remove Tests to open the Tests and Profiles
Programming Window.
9. Choose PT-RP, APTT-SS and Fib-C (choose appropriate tests for the other
controls).
10. Single click on the next available position on the Sample Rack and repeat
steps 4-9 for each QC material.
11. Single click or touch Insert Rack and load the Sample Rack onto the
analyzer.
NOTE: There should be NO ?’s on the newly loaded Sample Rack. If there are,
you forgot to choose the Insert Rack icon first. Remove the rack, double
click on the Offline Rack to reopen it, choose the Insert Rack icon then
reload the rack.
Repeat FAILED QC
Review QC Statistics
1. Single click on the words for any QC level on the Material/Tests Definition
Tree.
2. Single click or touch the Switch View icon to switch between the
following views:
2. Single click or touch Switch View until the Grid View (table of results)
is displayed.
3. Looking at the picture above, how do you know D-Dimer Low Control is
being displayed?
______________________________________________________________
4. Place your mouse over the word FAILED (4 on the screen capture above) in
the QC Status column.
5. What information is displayed?
______________________________________________________________
1. Single click or touch Switch View until the Chart View (Levey-
Jennings) is displayed.
1. Find a QC that has a failed result. Use any view you like.
2. Select the data point. Use the scroll bar at the bottom of the table of results to
find the Comment column then look for a result with a check in this column.
6. Use the scroll bar to scroll over to the Comment column then run your
mouse over the check in the column to view the comment. Using the Grid
view (table of results) is an easy way to locate a data point with a comment.
SKILL CHECK
________________________________________________________________________