GROUP 1

QUESTIONS FOR RESEARCH:

1. Which group of carbohydrates reacts most readily to the Seliwanoff’s Test?

- Polysaccharides, such as maltose, lactose, and sucrose, and oligosaccharides, like

fructose, galactose, and glucose, reacts most readily to the Seliwanoff’s Test.

2. Write the chemical equation involved in the reaction between concentrated HNO3 and

galactose.

CH2OH – (CHOH)4 – CHO + NHO3 -> CH2OH – (CHOH)4 – CHO – N03 + H+

3. Give other sugars that will give a positive result in the mucic acid test

- Only lactose and galactose will give a positive result in the Mucic Acid Test.

4. Give sugars that form characteristic osazone crystals. How do the crystals differ?

- Reducing sugars like some disaccharides and monosaccharides form characteristic

osazone crystals. These characteristic crystals differs in shape. Maltose forms petal-shaped
crystals. Lactose forms powder puff-shaped crystals. Galactose forma rhombic plate-
shaped crystals. Arabinose forms ball-shaped crystals. Monosaccharides like glucose,
fructose, and mannose produce needle-shaped osazone crystals.
5. Give the principle involved in the formation of an intense blue color formed in the Iodine test

for starch.

- Amylose present in starch is responsible for the blue color. The formation of the intense blue
color formed in the Iodine Test is the result of iodine molecules bound inside the amylose
helix
6. Give the reason why grinding of the sand and TCA is necessary in preparing glycogen.

- Animal tissue, the source for glycogen, is pretty tough and has to be broken down nicely so
that all glycogen is available for extraction. The grinding of sand and TCA (Tri-Chloro Acetic
Acid), a highly corrosive agent, aids in the breaking down of the tissue.
sucrose