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Lab Activity VIII: Regeneration
Lab Activity VIII: Regeneration
REGENERATION
Day : Thursday
Date : 15th November 2018
A. Aim
The aims of this practical class is to be able to observe the regeneration process
of nilem fish (Osteochilus vittatus) fins.
B. Benefits
The benefits of this practical class are able to know the regeneration process
especially from nilem fish (Osteochilus vittatus) and to know the ability of regeneration
in different nilem fish fin.
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II. MATERIALS AND METHODS
A. Materials
The tools that used in this practical class are scissors, millimetre block,
magnifying glass, sieve, and container.
The materials that used in this practical class are Nilem fish (Osteochilus
vittatus) with the high of 10-15 cm, pellets, and well water.
B. Work Procedures
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III. RESULT AND DISCUSSION
A. Result
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Figure 3.1 Fish Fin Before Being Cut Figure 3.2 Fish Fin Day-0
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A. Discussion
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The basic phase of regeneration that starts from wound healing, the
beginning of formation in the epidermis by the mass of mesenchymal cells called
blastema, then the cells multiply which eventually will reshape or restore the
morphology of the fish fin (Ochandio et al., 2015). Following cutting in the first
12 hours, the injured fin is repaired by rapid epidermal cell migration over the
cutting surface. The mesenchymal tissue is located around the part of the cutting
that is experiencing being organized and displays increasing cell proliferation.
This process is followed by the formation of blastema at 24-36 hours post
amputation (hpa). The regenerating structure enlarges and remains whitish until
the third to fourth day. Starting from the fifth to sixth day, the white tissue persists
only at the distal area of the outgrowth, while the proximal part of the new tissue
progressively redifferentiates into the mature fin fold and acquires pigmentation
(Pfefferli & Anna, 2015). Blastema makes an apical mesenchymal growth zone
treated under external skin wounds during the period of regenerative development,
which takes approximately 2 weeks. With prolongation of development, the
blastema cells proximal to exit this area and begin differentiating to correct the
lost structure with proxomodistal direction (Rasmussen & Sagasti, 2016).
The ability to regenerate in various organisms is not the same, there are high
and some are low (Yatim, 1990). Regeneration is a process that is so important
for the life of living things. In the body of living things there is the ability to
regenerate at the cellular or tissue level whereas in certain animals it can
regenerate at the organ level. Factors that affect the regeneration of fish are
internal and external factors. Internal factors such as the activity of the thyroid and
pituitary glands that regulate the level of regeneration, while external factors such
as temperature, certain light intensity, environmental contaminants and drugs such
as amino propionitril, penicillin, indomethacin, dexaminaton, and acidmay
acetalisate which interfere with the regeneration capacity of fins. Regeneration is
influenced by several factors including temperature, biological processes and food
ingredients. An increase in temperature, in certain cases, accelerates regeneration.
Regeneration is faster at 29.7oC (Radiopoetro, 1989).
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IV. CONCLUSION
A. Conclusion
Based on the result and discussion, it can be conclude that the fin
regeneration process occur in three phases, namely wound healing, formation of
blastema and regenerative development.
B. Suggestion
Need anesthetion for the fish, so it won’t stress and potentialy dead. When
measuring and observing make space and search for brigthter light.
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