LAMPIRAN 4

Perhitungan Pembuatan Bahan Uji

1. Kontrol Negatif (Na CMC 0,5 %)

0,5 𝑔𝑟𝑎𝑚
Stok = 0,5 % = 500 mg/100 ml
100 𝑚𝑙
0,5 𝑔𝑟𝑎𝑚
Volume Pemberian = x 5 ml = 5 ml
100 𝑚𝑙

2. Suspensi Uji Ekstrak etanol 200 mg/200g BB

𝐷𝑜𝑠𝑖𝑠 𝑥 𝐵𝐵 𝑚𝑎𝑥
Stok = 1
𝑉
2
200𝑚𝑔/200𝑔𝐵𝐵𝑥200𝑔
= 1
𝑥5 𝑚𝑙
2
200 𝑚𝑔
= 2,5 𝑚𝑙
= 80 mg/ml
= 4000 mg/50ml

𝐷𝑜𝑠𝑖𝑠 𝑥 𝐵𝐵 𝑚𝑎𝑥
Volume Pemberian = 𝑠𝑡𝑜𝑘
200𝑚𝑔/200𝑔𝐵𝐵𝑥200𝑔
= = 2,5 ml
800 𝑚𝑔/𝑚𝑙

3. Suspensi Uji Ekstrak etanol 250 mg/200g BB

𝐷𝑜𝑠𝑖𝑠 𝑥 𝐵𝐵 𝑚𝑎𝑥
Stok = 1
𝑉
2
250𝑚𝑔/200𝑔𝐵𝐵𝑥200𝑔
= 1
𝑥5 𝑚𝑙
2
250 𝑚𝑔
= 2,5 𝑚𝑙
= 100 mg/ml
= 5000 mg/50ml

𝐷𝑜𝑠𝑖𝑠 𝑥 𝐵𝐵 𝑚𝑎𝑥
Volume Pemberian =
𝑠𝑡𝑜𝑘
250𝑚𝑔/200𝑔𝐵𝐵𝑥200𝑔
= = 2,5 ml
1000 𝑚𝑔/𝑚𝑙

4. Suspensi Uji Ekstrak etanol 300 mg/200g BB

𝐷𝑜𝑠𝑖𝑠 𝑥 𝐵𝐵 𝑚𝑎𝑥
Stok = 1
𝑉
2
300𝑚𝑔/200𝑔𝐵𝐵𝑥200𝑔
= 1
𝑥5 𝑚𝑙
2
 300 𝑚𝑔
= 2,5 𝑚𝑙
= 120 mg/ml
= 6000 mg/50 ml

𝐷𝑜𝑠𝑖𝑠 𝑥 𝐵𝐵 𝑚𝑎𝑥
Volume Pemberian = 𝑠𝑡𝑜𝑘
300𝑚𝑔/200𝑔𝐵𝐵𝑥200𝑔
= = 2,5 ml
1200 𝑚𝑔/𝑚𝑙