FAMILY BACILLACAEA

DICHOTOMOUS KEY: GENUS TO SPECIES

Genus Bacillus
1a. Gram positive…………………………………………………………………................ go to 2
b spore forming…………………………………………………………………….……...go to 3

2a. classified strains are R-6540T (=LMG 22081T=DSM 16013T) ……………………. mojavensis
b) strains TF-11(T) and TF-12(T).................................................................................aquimaris
c) strains IDA3307T=R-15439T…...................................................................................novalis
d) strains L87-10T (= NRRL B-23154T) ....................................................................sonorensis
. e) strains DSM 4216T….........................................................................................................smithii
f) strain DV1-F-3 (5 NRRLB-14890)) .......................................................................valismortis
g) strain R-6540T…...................................................................................................farraginis
h) strains YKJ‐10, AF221061, JCM 10885, CIP 107104, KCCM 41040......................jeotgal
I) strains AM31D, IAM 15000, NCIMB 13904, JCM 11691.............................krulwichiae
j) strains ATCC 53909, DSM 10191, LMG 12887, KCTC 3742..........................naganoensis
k) strains FO‐92, ATCC BAA‐519, DSM 15077................................................nealsonii
l) strains PN‐3, DSM 8715, NCIMB 10283, LMG 17944, ATCC 700159.........pseudofirmus

3a) aerobic...............................................................................................................................go to 5
b) facultative aerobic.............................................................................................................go to 7
c) rod shaped……………………………………………………………………………….go to 6
d) perch shaped……………………………………………………………………………..go to 4

4a. considered pathogenic…………………………………………………………………..cerceus

b. considered non-pathogenic……………………………………………………………….fordii

5a. black-pigmented bacteria……………………..………………………………………atrophaeus

b. red-pigmented bacteria …………………………..………………………………...megatarium
c) produces grayish yellow pigment .................................................................... carboniphilu
d) produces semitransparent.........................................................................................algicola
e) produces cream‐white to pale yellow in color .........................................................clarkii
f) produces white or pink‐redt.....................................................................................ampicillin
g) soft‐brown pigment ................................................................................................bataviensis
h) pink and red pigmentation…...........................................................................endophyticus
i) creamy‐brown, and butyrous....................................................................................fumarioli
j) smooth, flat and butyrous …......................................................................galactosidilyticus
k) yellowish‐orange pigmented.......................................................................................indicus
l) soft, off‐white and irregular …...................................................................................insolitus
m) cream‐colored, produce a soft brown pigment............................................................neidei
n) round, smooth, flat with entire edges and beige in color ...................................odysseyi

6a. grow at NaCl concentrations of 0.5-25 % (w/v) ..............................................halosaccharovorans

b) grow occurred with between 0.5 and 7.5% (w/v) NaC..................................................salsus
c) grew optimally in 1 % trypticase soy broth (TSB)) ..............................................decisifrondis
d) grew optimally at 30-37 degrees C and pH 7.0-8. 0...............................................pallidus
e) growth lies between 58°C and 60°C............................................................................gelatini
f) Growth temperature range 15–55°C..............................................................halodurans
g) Optimal growth at pH 10.0 ...................................................................................agardhaerans
h) Optimal growth at pH 9.0–10. 0.....................................................................alchaphilus
 i) growth requirements are complex; non‐fermentative) .......................................azotoformans
j) growth is weak at pH 6. 0....................................................................................barbaricus
k) growth is 0–10% (w/v), with an optimum of 4–7% (w/v) ....................................decolorationis
l) Grows in presence of 5% NaCl...........................................................................fastidiosus
m) growth is 6.0–7.0.................................................................................................. hwajinpoensis
n) growth at pH 8–10....................................................................................................hortim)
o) Growth occurs at 28–37°C, pH 7–8.4 and 0.12–3% NaCl......................................... macyae
p) grow in presence of 7% NaCl..............................................................................mycoides

7a cell width 0.8–0.9 µm in diameter..................................................................................................flexus

b ) cell width 0.9 µm...........................................................................................................firmus
c)Cells 0.6–1.0 by 2.0–3.0 µm.....................................................................................................gibsonii
e) Cells 0.6–1.0 by 3.0–4.0 µm....................................................................................halmapalus