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Biosystems Engineering (2004) 89 (1), 109–117

doi:10.1016/j.biosystemseng.2004.06.012
PH—Postharvest Technology

Some Physical Properties of Amaranth Seeds


R. Abalone1; A. Cassinera2; A. Gastón3; M.A. Lara2
1
Fac. de Cs. Exactas Ingenierı́a y Agrimensura, UNR. IFIR (CONICET/UNR), Av. Pellegrini 250, 2000 Rosario, Argentina;
e-mail of corresponding author: rabalone@fceia.unr.edu.ar
2
IFIR (CONICET/UNR), Av. Pellegrini 250, 2000 Rosario, Argentina
3
Consejo de Investigaciones de la UNR, Fac. de Cs. Exactas Ingenierı́a y Agrimensura. UNR, Av. Pellegrini 250, 2000 Rosario,
Argentina; e-mail: analiag@fceia.unr.edu.ar

(Received 13 November 2003; received in revised form 22 June 2004; published online 20 August 2004)

Abstract

Several physical properties of amaranth seeds (Amaranthus cruentus) were evaluated as a function of moisture
content. When moisture content changed from 77 to 439% d.b., true density decreased from 1390 to 1320 kg
m3, bulk density from 840 to 720 kg m3, specific volume increased from 078 to 110  103 m3 kg1 and
porosity varied from 040 to 045. Shrinkage coefficient was found to be 125. By means of image analysis,
relevant dimensions were measured as a function of moisture content. In the moisture content range of
95–436% d.b., the average length, width and thickness were 142, 129 and 087 mm, respectively. The
geometric mean diameter of seed ranged from 110 to 124 mm, whereas mean sphericity was 082. The average
pycnometric volume and area of one grain were 065 mm3 and 396 mm2, respectively.
r 2004 Silsoe Research Institute. All rights reserved
Published by Elsevier Ltd

1. Introduction and Universidad Nacional Rı́o Cuarto, Córdoba),


where research programs focused on agronomic aspects
Amaranths grains, rediscovered by modern agricul- are being conducted to promote the cultivation.
ture, have remarkable nutritional properties because of Amaranth grain yields are extremely variable dependent
the amount and quality of protein (16–18%) and of upon cultivar selection and the growing season, parti-
lysine and triptophan content, in low proportion in cularly with regard to available soil moisture. Tosi and
other cereals. Owing to the outstanding nutritional, Ré (2003) have reported grain yields from 1800 to
industrial and pharmacological applications, interna- 2300 kg ha1 and exceptionally, 4500 kg ha1.
tional demand for this grain is increasing. Amaranth is Proper postharvest handling is critical for producing
common in Peru, Bolivia and Mexico. In the United high-quality grain and appropriate technology for this
States, about 2400 ha are planted in Great Plains and new crop now requires to be developed.
Midwest states, but the largest producer is China, Due to the smallness of amaranth, conventional
yielding 86 000 tones per year (Ag Innovation News, drying systems are not adapted to the characteristics
2003). of this seed and the use of silos for drying is limited
Argentina has potential areas suitable for the cultiva- because of the important pressure drop developed
tion of amaranth located mainly in Jujuy, Santiago del through the depth of the grain bed. To adapt an existing
Estero, Córdoba, Eastern La Pampa and Western industrial dryer or design a new one, physical and
Buenos Aires (Covas, 1994). At present, the growth is transport properties or drying parameters of the grain
reduced to experimental stations (Universidad Nacional must be known, and the available information on these
de La Pampa, Estación Anguil (INTA) in La Pampa topics is rather scarce (Tosi et al., 1999; Lema et al.,

1537-5110/$30.00 109 r 2004 Silsoe Research Institute. All rights reserved


Published by Elsevier Ltd
110 R. ABALONE ET AL.

Notation

A, B, C length, width, thickness of grain, mm W moisture content, % d.b.


De equivalent diameter, mm Wi initial moisture content, % d.b.
Dg geometric mean diameter, mm b deep bed porosity, dimensionless
e1 , e2 semi-axis of oblate spheroid, mm b; b volume shrinkage coefficient, dimensionless
e3 ; e4 ; semi-axis of solid of revolution, mm rt true density of moist amaranth grains,
e5 ; e6 kg m3
mgrain total mass of sample, g r0 density at a moisture content of 0% d.b.,
ms dry mass, g kg m3
mw mass of water, g rs density of dry solids, kg m3
m1 dry mass of one kernel, mg rw density of water, kg m3
N grain number, dimensionless rb bulk density or apparent density of a deep-
R2 coefficient of determination, dimensionless bed of grains, kg m3
S oblate surface area of oblate spheroid, mm2 n specific volume (volume per unit of dry
S solid surface area of solid of revolution, mm2 matter), m3 kg1
S sph surface area of equivalent sphere, mm2 n0 specific volume at a moisture content of 0%
Va volume of air, mm3 d.b., m3 kg1
Vb bulk volume, mm3 ni specific volume at initial moisture content,
Vg pycnometric volume of one kernel, mm3 m3 kg1
V grain volume of grains, mm3 f grain sphericity, dimensionless
Vs volume of dry solids, mm3 x eccentricity, dimensionless
Vw volume of water, mm3

2001; Calzetta Resio et al., 2003; Vizcarra Mendoza The seeds were divided into lots and they were
et al., 2003). Size, surface area and volume are required conditioned to obtain moisture contents in the moisture
in different handling and processing operations and are content range of 7–44% d.b. by adding pre-determined
also needed as input parameters for the prediction of quantities of distilled water and mixing for 30 min. The
transport properties and drying rates of grains through prepared samples were sealed in hermetic flasks and
simulation models. In particular, the estimation of the stored in a refrigerator for 2 days for the moisture to
effective diffusion coefficient of water is affected by the distribute uniformly throughout the sample.
geometry assumed to approximate the grain shape Before the start of each test, the samples were
(Gastón et al., 2002; Kang & Delwiche, 2000). removed from the refrigerator and allowed to equili-
The objective of present work is to measure some brate to room temperature. The moisture content
physical properties, such as true density, bulk density, was determined using the ASAE standard method
specific volume, porosity, shrinkage coefficient, geo- (ASAE 1984). Oven temperature and heating period
metric dimensions, volume and surface area of amar- were set at 901C and 48 h (Lema et al., 2001),
anth seeds. respectively.

2.2. Physical properties of amaranth seeds


2. Materials and methods
A standard pycnometric method (Mohsenin, 1970)
2.1. Sample preparation was used to determine the volume of a weighed sample
for different moisture contents (pycnometer volume:
Amaranth seeds (Amaranthus cruentus) from the 2000 (100701) ml; toluene density: (880710) kg m3). The
crop at the experimental station of Universidad Nacio- mass of the sample was measured by means of an
nal de La Pampa, La Pampa, Argentina, were used in electronic balance (Mettler, type H5) reading to 0001 g.
the tests. Amaranth is currently harvested at about 30% Another grain sample was poured into a vessel of known
d.b., and then dried to 11% d.b. for preservation. The volume (108 ml) and weighed to determine bulk density.
grain was hand-cleaned to remove any foreign material Five replicates were conducted for each moisture
before being stored in plastic bags. content.
SOME PHYSICAL PROPERTIES OF AMARANTH SEEDS 111

A simple parametric model was used to describe the grain porosity is zero, r0 is equal to the density of dry
dependence of physical properties with moisture content solids rs .
(Zogzas et al., 1994). The density of the moist amaranth Bulk density rb or apparent density of a deep-bed of
grains or true density rt in kg m3 is defined as: grains is defined by:
mgrain ms þ mw mgrain
rt ¼ ¼ ð1Þ rb ¼ ð8Þ
V grain V s þ V w þ V a Vb

where mgrain is mass of the sample in g; Vgrain is volume where V b is the bulk volume in mm3 including volume of
of grains in mm3; ms is dry mass in g; mw is mass of void spaces.
water in g; and Vs, Vw and Va, are the volumes in mm3 Deep bed porosity is:
of dry solids, water and air, respectively. It was assumed r
b ¼ 1  b ð9Þ
that the mass of air is negligible. rt
Density of dry solid rs in kg m3 is defined by:
ms 2.3. Geometrical dimensions of amaranth seeds
rs ¼ ð2Þ
Vs
In order to determine the size and shape of the seeds,
and the specific volume n (volume per unit of dry matter)
the images of individual grains were acquired. A 23 inch
in m3 kg1 by:
charge-coupled device (CCD) video camera (XC-711P,
V grain Sony Corp., Japan) was used (Granitto et al., 2002),
n¼ ð3Þ
ms connected to a colour frame grabber (IC-PCI, Imaging
Technology Inc., USA) with 8-bit reference tables per
The volume shrinkage coefficient b is defined by the
colour channel. Illumination was provided by a 150 W
equation:
  light source (Fostec Inc., USA) through a quadruple
W fibre optic bundle of 127 mm diameter, with the four
n ¼ n0 1 þ b ð4Þ
100 guides in a symmetric arrangement to produce an even
illumination with good texture enhancement.
where W is moisture content in % d.b. and n0 is specific Grains were chosen at random and exposed to the
volume in m3 kg1 at a moisture content of 0% d.b. camera with two orientations: horizontally and verti-
Other researchers (Saravacos & Maroulis, 2001) cally, as shown in Fig. 1. Images were taken with a
define the volume shrinkage coefficient by the following 768  512 pixel resolution on a blue background, which
relationship, which represents the proportion of initial can be easily subtracted by standard segmentation
specific volume that shrinks as water is removed: routines because of the difference in colour with the
b ðW i  W Þ seeds. From the binarised images, length A, width B and
n ¼ ni  ð5Þ
rw 100 thickness C, in mm, were determined (Granitto et al.,
2002; Keefe et al., 1986; Yutaka et al., 1982).
where W i is the initial moisture content of the moist Geometric mean diameter Dg in mm and sphericity f
food material in % d.b., ni is the specific volume at values were found using the following expressions
initial moisture content in m3 kg1 and rw is water (Mohsenin, 1970):
density in kg m3. The symbol b is introduced to
1
identify this coefficient from the previous one. The Dg ¼ ðABC Þ3 ð10Þ
shrinkage coefficient b thus defined varies between 0
(no shrinkage) and 1 (full shrinkage). Both coefficients 1
ðABC Þ3
are simply related by: f¼ ð11Þ

A
b
b¼ ð6Þ
n0 rw
2.4. Volume, shape and surface area of amaranth seeds
Combining Eqns (1) and (3), leads to the following
non-linear relationship between the grain density and
If the number of grains N contained in the sample is
moisture content:
known, the pycnometric volume of one grain V g in mm3
r0 ð100 þ W Þ can be determined from the pycnometric volume of the
rt ¼ ð7Þ
ð100 þ bW Þ sample V grain :
where r0 is the density at a moisture content of 0% d.b. V grain
Vg ¼ ð12Þ
If the volume of air pores V a is negligible so that the N
112 R. ABALONE ET AL.

Fig. 1. Image of amaranth grain with principal measured


dimensions: A, length; B, width; C, thickness

The shape of amaranth (Fig. 1) was approximated by


three geometric shapes illustrated in Fig. 2(a)–2(c), being
in all cases the volume of geometric shapes the same as
the grain pycnometric volume V g .
In the first case [Fig. 2(a)], an equivalent sphere was
assumed and the equivalent diameter De in mm was
determined by:
 1
6 3
De ¼ Vg ð13Þ
p

Evaluation of grain surface is straightforward and will


be denoted by S sph , in mm2.
In the second case [Fig. 2(b)], by rotating an ellipse
about its minor axis an oblate spheroid was generated to
approximate the grain shape. The long semi-axis e1 of
the ellipse in mm was chosen to be the average of Fig. 2. Approximation of amaranth shape: (a) sphere; (b)
measure dimensions A and B: oblate spheroid; (c) solid of revolution; De, equivalent diameter;
  e1 to e6, semi-axes
1 AþB
e1 ¼ ð14Þ
2 2
SOME PHYSICAL PROPERTIES OF AMARANTH SEEDS 113

and the short semi-axis e2 in mm was calculated so that Table 1


the geometric volume of the ellipsoid equals the Mean measured values of gravimetric properties of amaranth in
the moisture content range of 7 . 7–43 . 9% d.b.
pycnometric volume Vg of the grain:
4 Moisture Total mass Dry mass Volume Grain
V g ¼ pe2 e21 ð15Þ content % d.b. (mgrain), g (ms), g (Vgrain), Number
3 mm3 (N)
The surface area of an oblate spheroid Soblate in mm2
77 7114 66 5100 9500
can be calculated to estimate the grain surface, accord- 148 7006 610 5200 8760
ing to the following equation (Mohsenin, 1970): 172 7143 609 5200 8750
206 7105 589 5200 8460
e22 1þx
S oblate ¼ 2p e21 þ p ln ð16Þ 225 7071 577 5300 8290
x 1x 256 6636 528 4900 7580
290 6333 491 4700 7050
where the eccentricity x is given by:
353 6349 469 4800 6740
sffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffi
 2 439 6163 428 4700 6150
e2
x¼ 1 ð17Þ
e1
In the third case, in order to improve the geometric
approximation of the actual shape of amaranth, two volume increased from 078 to 110  103 m3 kg1
intersecting ellipses were used to fit the grain silhouette and bulk density decreased from 840 to 720 kg m3 with
as closer as possible [Fig. 2(c)] and then rotated about the increase of moisture content from 77 to 439% d.b.
the minor axis to generate a solid of revolution. One The following linear correlations were derived from
ellipse is characterised by semi-axes e3 and e4, and the data:
other by semi-axes e5 and e6. Two of these semi-axes
were chosen to be equal to mean measured dimensions: n ¼ 071  103 ð1 þ 00125 W Þ ð20Þ
 
1 AþB
e3 ¼ ð18Þ
2 2 rb ¼ ð869  350 W Þ ð21Þ

C
e6 ¼ ð19Þ with values for the coefficient of determination R2 of
2 099 and 087, respectively.
The other two semi-axes, e4 and e5, were determined The results for bulk density are in accordance with
so as to make the relative difference between geometric those presented for sunflower (Gupta & Das, 1997),
volume and grain pycnometric volume negligible guna (Aviara et al., 1999), green gram (Nimkar &
(5  104%) in comparison to the relative error (5%) Chattopadhyay, 2001), Turkish mahaleb (Aydin et al.,
involved in the grain pycnometric volume measurement. 2002) and cotton (Özarslan, 2002). Vizcarra Mendoza et
Geometric volume and surface areas of the solid of al. (2003) reported for the variety Amaranthus hipocon-
revolution were calculated by rotation of the area and driacus 860 kg m3 for bulk density, without reference of
the boundary shown in Fig. 2(c) about the Y axis of the moisture range considered. Compared to quinoa
symmetry, respectively. seed, a pseudocereal of similar characteristics, in the
moisture content range of 7–25% d.b., amaranth bulk
density was 15% higher than those reported by Vilche
3. Results and discussion et al. (2003).
Comparing Eqn (20) with Eqn (4) the shrinkage
3.1. Physical properties of amaranth seeds coefficient b and the specific volume n0 were determined,
giving values of 125 and of 071  103 m3 kg1,
Table 1 shows the mean measured values of mass of respectively. b is calculated from Eqn (6), giving a
sample mgrain, dry mass ms and volume of sample Vgrain value of 089. The density r0 (the reciprocal of n0 ) has a
together with the number of grains N contained in each value of 1411 kg m3.
sample. Figure 5 displays true density rt as function of
Applying Eqns (1), (3), (8) and (9) true density rt , moisture content. True density decreased from 1390 to
specific volume n, bulk density rb and porosity b were 1320 kg m3 in the moisture range of 77–439% d.b.
determined for each moisture content. Vizcarra Mendoza et al. (2003) reported for Amaranthus
Figures 3 and 4 display specific volume n and bulk hipocondriacus a value of 1370 kg m3. In order to verify
density rb as function of moisture content. Specific the parametric model presented previously (Section 3.2),
114 R. ABALONE ET AL.

1.2 1420

1400
-1

1.1
Specific volume v, 10-3m kg

True density  , kgm-3


3

1380
1.0

i
1360

0.9 1340

0.8 1320

1300
0.7
10 20 30 40 10 20 30 40
Moisture content W,% d.b. Moisture content W,% d.b.

Fig. 3. Specific volume as function of moisture content. Fig. 5. True density as function of moisture content.
, experimental data; —— , linear fit , experimental data; —— , predicted values with Eqn (22)

800 0.48

840
Bulk density  , kgm-3

0.44
800
Porosity b
b

760
0.40

720

0.36
680
10 20 30 40 10 20 30 40
Moisture content W,% d.b. Moisture content W,% d.b.
Fig. 4. Bulk density as function of moisture content. Fig. 6. Deep-bed porosity as function of moisture content.
, experimental data; —— , linear fit , experimental data; —— , linear fit

b and r0 values were introduced in Eqn (7) giving: Deep bed porosity varied from 040 to 045 (Fig. 6),
showing an increasing linear dependence with moisture
1411ð100 þ W Þ
rt ¼ ð22Þ content expressed by:
ð100 þ 125 W Þ
b ¼ ð038 þ 00016 W Þ ð23Þ
True density calculated with Eqn (22) was compared
to measured values in Fig. 5 showing small deviations with a value for the coefficient of determination R2 of
between them. True density bears a non-linear decreas- 075.
ing relationship with moisture content, different from In the moisture content range of 77–439% d.b.,
the linear decreasing trend reported for guna (Aviara et porosity only increased 125% in contrast with the wide
al., 1999), green gram (Nimkar & Chattopadhyay, variation reported for quinoa (Vilche et al., 2003) of
2001), Turkish mahaleb (Aydin, 2002) and cotton about 1258% in the moisture range of 46–258% d.b.
(Özarslan, 2002) and opposite to the increasing linear On average porosity of amaranth was found to be of the
trend reported for quinoa (Vilche et al., 2003) and same order as wheat, shelled corn and sorghum
sunflower (Gupta & Das, 1997). (Brooker et al., 1974).
SOME PHYSICAL PROPERTIES OF AMARANTH SEEDS 115

Table 2
Mean measured dimensions of amaranth grains in the moisture content range of 9 . 5–43 . 6% d.b.

Moisture content Length (A), Width (B), Thickness (C), Geometric Mean Diameter Sphericity
% d.b. mm mm mm (Dg), mm (f)
95 135 122 081 110 081
155 140 126 084 114 082
210 140 127 087 115 083
305 144 129 089 118 082
436 150 137 093 124 083

3.2. Geometrical dimensions of amaranth seeds

The size of the seeds were determined at five different 0.8

Pycnometric volume Vg , mm3


moisture content level of 95, 155, 210, 305, 436% d.b.
For each moisture content, 40 kernel were measured,
mean values of dimensions A, B and C were determined 0.7
(Table 2) and correlated with moisture content giving
the following equations:
A ¼ ð1319 þ 000405 W Þ ð24Þ 0.6

B ¼ ð1183 þ 000409 W Þ ð25Þ


0.5
C ¼ ð0787 þ 000335 W Þ ð26Þ
with values for the coefficient of determination R2 of 10 20 30 40
096, 096 and 095, respectively. Moisture content W,% d.b.
Geometric mean diameter and sphericity (Table 2)
Fig. 7. Pycnometric volume of one grain as function of moisture
were found to bear a linear relationship with moisture
content. , experimental data; —— , linear fit
content expressed by the following correlations:
Dg ¼ ð1071 þ 000385 W Þ ð27Þ
determine the mass of one dried grain, the mass of a
f ¼ ð0813 þ 0000365 W Þ ð28Þ sample of 15 000 dried grains was measured by
both with a coefficient of determination R2 of 099. analytical balance (Mettler, type H5), giving m1 a value
In the range of moisture content considered of (69637001) mg. The variation of one grain volume
(95–436% d.b.) geometric mean diameter increased with moisture content was represented by the following
127% varying from 110 to 124 mm. Amaranth correlation depicted in Fig. 7:
sphericity has an average value of 082, which is 5% V g ¼ ð0493 þ 000611 W Þ ð29Þ
higher than the averaged value reported for quinoa of
078 in the moisture content range of 46–258% d.b. with a value for the coefficient of determination R2 of
(Vilche et al., 2003). Amaranth sphericity increased 099.
12% exhibiting a weaker dependence with moisture The characteristic dimensions of the different geo-
content than the one obtained for quinoa of 32%. metric shapes, selected to approximate the seed shape
were correlated with moisture content.
The equivalent diameter of the sphere having the
3.3. Volume, shape and surface area of amaranth seeds
same pycnometric volume of the grain was expressed by:
The pycnometric volume of one grain was calculated De ¼ ð0987 þ 000341 W Þ ð30Þ
from the measured pycnometric volume of the samples
presented in Table 1 using Eqn (12). The number of with a value for the coefficients of determination R2 of
grains N contained in a sample (listed in Table 1) was 099.
estimated as the ratio of the known dried mass of the If the grain is represented by an oblate spheroid, the
sample ms to the mass of one dried grain m1. In order to ellipsoid semi-axis dependence with moisture content
116 R. ABALONE ET AL.

Table 3
Measured pycnometric volume and calculated geometric area of one grain in the moisture content range of 9 . 5–43 . 6% d.b.

Moisture content % Volume (Vg), Equivalent sphere Oblate spheroid area Solid of revolution
d.b. mm3 area (Ssph), mm2 (Soblate), mm2 area (Ssolid ), mm2

95 055 326 359 360


155 059 340 373 374
210 062 353 386 388
305 068 375 410 412
436 076 404 443 447

were found to be: to 720 kg m3 and porosity varied from 040 to 045.
e1 ¼ ð0625 þ 000203 W Þ ð31Þ Shrinkage coefficient was found to be 125. If it is
defined as the proportion of initial specific volume
e2 ¼ ð0308 þ 000119 W Þ ð32Þ that shrinks as water is removed, was found to
2 be 089.
with values for the coefficient of determination R of Relevant dimensions of the grain were measured and
096 and 098, respectively. correlations with moisture content were obtained in the
In the solid of revolution case, the semi-axis of the moisture content range of 95–436% d.b. On average,
ellipsoids were found to bear the following relationship length, width and thickness were 142, 129 and 087 mm,
with moisture content: respectively. The geometric mean diameter of seed
e3 ¼ ð0625 þ 000203 W Þ ð33Þ varied from 110 to 124 mm, whereas mean sphericity
was 082. The average pycnometric volume of one grain
e4 ¼ ð0231 þ 000098 W Þ ð34Þ was 065 mm3.
Three different geometries (equivalent sphere, oblate
e5 ¼ ð0481 þ 000157 W Þ ð35Þ spheroid, solid of revolution) having the same pycno-
metric volume as the grain were assumed to approx-
e6 ¼ ð0393 þ 000168 W Þ ð36Þ
imate amaranth shape and calculate the grain surface
2
with values for the coefficient of determination R of area. Based on the solid of revolution shape, the
096, 095, 088 and 091, respectively. estimated surface area was on average 396 mm2, being
Table 3 lists the grain pycnometric volume and this values 10% greater than that of the equivalent
calculated surface areas associated to each representa- sphere.
tion. Results show that the surface area calculated with
the solid of revolution is 10% greater than the surface of
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