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Type X

 Methylococcus capsulatus
 Price: £239
Details: https://www.ncimb.com/BioloMICSDetails.aspx?Table=Website%20catalogue&Rec=102580
Media : 131
Gas regime: CH4/Air/CO2 9:9:2
Incubation temperature (and/or range): 45°C
Growth factors (and/or information): NMS, 45C, 4d: fairly good growth

Type II
 Methylosinus trichosporium OB3b NCIMB 11131 (obligate –utilization of CH4)
https://www.ncimb.com/BioloMICS.aspx?Table=Website%20catalogue&info=Help%20on%20queries
Price: £239
Media given by NCIMB: 131  https://www.ncimb.com/TempFiles/20190310/TempF131_C131.pdf
Gas regime: CH4/Air 1:1
Incubation temperature (and/or range): 30°C
Growth factors (and/or information): NMS, 30C, 7d: fairly good growth 

https://www.ncimb.com/BioloMICSDetails.aspx?Table=Website%20catalogue&Rec=102607
Type X
 Methylomonas methanica
 Price band : Band B active (not available freeze-dried) £478
 Gas regime: CH4/Air 1:1
 Incubation temperature (and/or range): 30°C
 Growth factors (and/or information): Does not grow on VCR medium NMS, 30C, 7d: fairly good
growth, colonies adhere quite strongly to the agar
Agar plates (UK orders):

 Pre-made: https://www.coleparmer.co.uk/i/prepared-agar-plate-standard-methods-agar-10-
pk/1420144?PubID=UY&persist=true&ip=no&keyword=&&gclid=Cj0KCQjwjpjkBRDRARIsA
Kv-0O3Hj8sWx9xPIRh9WWpw6DGmQ4dOHJcetn-V-
oSwP7l_fA2c4Z9ZfY8aAqeKEALw_wcB&gclsrc=aw.ds

 Long list of options: https://uk.vwr.com/store/product/16067986/pre-poured-plates

 Make your own: https://www.buywholefoodsonline.co.uk/agar-agar-


500g.html?gclid=Cj0KCQjwjpjkBRDRARIsAKv-
0O0jSqwzA5JHUN7sjnUu_s6m26EraJ0dLzxIxOjjR9-CkCg7DlTfGh4aAslwEALw_wcB

Other materials:

1. https://www.scilabware.com/en/mbl-
burettes-class-b-with-glass-key-1

2. Desiccator with https://www.scilabware.com/en/pyrex-


vacuum desiccator-with-vacuum-connection-1
connection

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