Migoto ProBac 9 for

Aquaculture

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 Bacteria in Migoto
ProBac 9

• Bacillus coagulans strain M203 & Bacillus subtilis strain M202 are
the active ingredients used.

• These strains stimulate shrimp and fish, and improves its immune
and digestion systems.

• Both live or dead show the same beneficial action to fish and shrimp.

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 Strong points of Migoto ProBac 9

1. It contains lactic acid & Bacillus strains

2. It stimulates immune-system and promotes digestion.

3. It is heat-stable that it can be mixed into a feed during

manufacturing process.

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 Shrimp Data

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Migoto ProBac 9 enhances shrimp growth rate

Survival Rate Body Weight Gain

100 140

130
90

Body Weight Gain(%)

120
Survival rate (%)

80

110
70
100

60
90

50 80
Control D1813 Control D1813

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 Migoto ProBac 9 promotes the expression
of digestion enzyme-related genes

Hepatopancreas Intestine (mid-gut)

Relative ixpression of target/EF1-α nRNA

Relative expression of target/EF1-α mRNA

5 5
control control
4 4
Migoto Migoto

3 3

2 2

1 1

0 0

The expressions of the genes encoding trypsin, chitinase, cathepsin B, cathepsin C were examined with real-time
PCR.

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Migoto ProBac 9-fed Shrimp shows stress-
resistance
Freshwater Stress Resistance of Juveniles After
30 days of Feeding Trial
4
Comulative Mortality Index

0
Control Migoto

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 Migoto ProBac 9 stimulates the immune
system of the shrimp
Immunostimulatory effects of Migoto ProBac 9 during 30 days feeding trial

Total Hemocyte Count Viable Cells Phagocytic Activity

8 100 60

Phagocytic activity (%)

Total hemocyte count

6 95
Viable Cells (%)
(×106 cell/ml)

40

4 90

20
2 85

0 80 0
0 10 20 30 0 10 20 30 0 10 20 30
Time of feeding (day) Time of feeding (day) Time of feeding (day)

Control
Migoto

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 Migoto ProBac 9 promotes the expression
of immune-related genes

Hepatopancreas Intestine (mid-gut)

7 7
Relative expression of target/EF1-α mRNA

Relative expression of target/EF1-α mRNA

control
6 control 6 Migoto
Migoto
5 5

4 4

3 3

2 2

1 1

0 0

The expressions of the genes encoding lysozyme, crustin, prophenoloxidase (proPO), Anti-
lipopolysaccharide factor (ALF) , Toll-1 and Toll-2 were examined with real-time PCR.

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Artificial infection with shrimp pathogen
Vibrio penaeicida
100%

80%
Survival rate (%)

60%

40%

20%

0%

Non- treatment Migoto

Shrimp was fed Migoto ProBac 9 containing feed 7 days after Vibrio
inoculation. 10
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 Field experiment of Migoto ProBac 9 on shrimp
aquaculture

Product : Migoto ProBac 9

Farming Species : White shrimp (Litopenaeus vannamei) farming
Type of Culture : Intensive culture

Food conversion ratio Average body weight Productivity

(FCR) (g/shrimp) (ton/ha)

1.8 28 20

1.7 1.75 26 19.65

26.1 19
1.6 24
18
1.5 1.55 22
21.4 17.58
1.4 20 17
Control Migoto Control Migoto Control Migoto

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 Fish Data

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 Migoto ProBac 9 stimulates the production of
immune-factor
Tiger puffer
Immuno-sitimulation TNF-

Leucocyte Migoto TNF-N
More than 5 times
IL-10
＋ IL-4/13A
IL-4/13B
IL-21
IFN-
(1 x 107 cells/ml) (1 x 109 cells/ml)

TGF-1
CSF-1b

Migoto can stimulate leucocyte directory and promote gene expression of

various important immune-factors.

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Growth and feeding performance of red tilapia Oreochromis
niloticus fed with practical diet containing Migoto ProBac 9

Parameter1 Control Migoto

(n=50) (n=50)
Initial weight (g) 13.8 ± 0.2 13.8 ± 0.2
Final biomass (kg) 8.07 ± 0.7a 12.03 ± 0.61c
Survival (%) 91 ± 1a 99 ± 1b
SGR2 (%/day) 1.69 ± 0.06a 1.90 ± 0.02c
FCR3 3.50 ± 0.29a 2.31 ± 0.12c
Protein retention (%) 16.25 ± 1.34a 23.34 ± 1.25c
Lipid retention (%) 17.91 ± 1.41a 48.99 ± 2.52d

1 Different
superscript following the mean value within the same parameter row indicate significant difference
2 SGR: specific growth rate
3 FCR: feed conversion ratio

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 Phagocytic Index of blood from Migoto –fed
tilapia before and after the artificial infection

12
Control
Migoto
10
Phagocytic Index (%)

0
Before After
Infection with Aeromonas hydrophylla

15
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Respiratory burst of blood from Migoto–fed tilapia
after infection with Aeromonas hydrophylla

Respiratory burst (absorbance/ml) 15

10

0
Control Migoto

16
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Artificial Infection with fish pathogen Aeromons hydrophyla

100
Survival rate (%)

75 96.67

50
53.3
25

0
Non-treatment Migoto

Survival of red tilapia (Oreochromis niloticus) fed with practical diet containing
Migoto and post challenged with Aeromonas hydrophyla (106 cell/mL, 2 weeks).
Each bar indicates significant difference (P<0.05).

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 Summary

1. Migoto Probac 9 is mainly composed of Bacillus coagulans M203 &

Bacillus subtilis M202.

2. Migoto Probac 9 showed immunostimulatory activity and promotes

growth rate.

3. Migoto Probac 9 -fed fish and shrimp show resistance to pathogens.

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