JOURNAL REVIEW

Isolation and Partial Characterization of Bacteria Activity Associated with

Gorgonian Euplexaura sp. Against Methicillin-Resistant Staphylococcus
aureus (MRSA)

LECTURER:
Nimas Mayang Sabrina Sunyoto STP., MP., M.Sc

ARRANGED BY
Ilmy Septa Islami 185100301111006
Novy Aprillia Eka Sari 185100301111019
Devina Permata Dewi 185100301111021

JURUSAN TEKNOLOGI INDUSTRI PERTANIAN

FAKULTAS TEKNOLOGI PERTANIAN

UNIVERSITAS BRAWIJAYA

MALANG

2019
 Title Isolation and Characterization of Lactic Acid Bacteria from Inasua
Journal Type Journal of Tropical Biodiversity and Biotechnology
Volume & Pages Vol. 1, Issue 2, 71-76
Year 2016
Ferymon Mahulette, Nisa Rachmania Mubarik, Antonius Suwanto,
Writer
Widanarni
Reviewer Mahdan Rizqi Pratama, Saiful Islam, Andre Baruna Anggaraksa
This research is to isolate and identify the characteristic of lactic
Research goals
acid bacteria in inasua.
Samples of inasua that were processed using three different types
of fish. These three samples are Samandar inasua, Gala-gala inasua,
Subject
Inasua sikuda. The fish has get trough fermentation for 5 months
and taken from people at TNS-Waipia District, Cream Island.
Methods that used in this research are dilution method to isolate the
bacteria, streak method to conduct the bacteria, overlay method to
Research Method
investigate the activity of antibacterial, and molecular identification
to identify the potent bacteria.
Methicillin-Resistant Staphylococcus aureus (MRSA), Euplexaura
Variables
sp., ciprofloxacin, erythromycin, clindamycin, Strain PG-344
Gorgonian Euplexaura sp. were collected from Panjang Island,
Jepara, Indonesia as the sample of this research. The sample were
rinsed by sterile sea water and kept inside cool box. Gorgonian
identification was done by identify the sclerit. One gram of the
sample was shattered slowly and then the homogenate that occur
from sample, was diluted with sterile sea water at 4 dilutions (10-1,
10-2, 10-3, 10-4). 1 ml from each dilution, was inoculated to nutrient
Steps
agar by spread plate method and being incubated for 2 days at 29oC.
The purification bacteria separated based on their colors, texture,
and shape of the colonies. The selective colony moved to nutrient
agar using inoculum method. Secondary metabolite is being filtered
by overlay method. It was done by bacterial activity that inoculated
inside nutrient agar toward MRSA. Bacterial association being
incubated for a night at 28oC. S. aureus inoculated on nutrient broth
 medium and grown a night at 27oC based on the condition. S.
aureus poured on nutrient agar plate which contains of bacteria to
indicate the activity of antibacterial. The active isolate created a
stain that can be identified the biological morphology. The
observation activity use microscope with magnification 100 X in
microscope.
Samples were identified by observation using a microscope at 10x
magnification. Euplexaura sp. has 3 forms sclerites. They are a
strong club, and a spindle rod and a reddish color. Each type has a
gorgonian sclerites which is distinct. Basic forms sclerites include
double head, spindle, club, scaphoid and rod. Sclerites form of a
specific character possessed by each type of gorgonian can be used
as a basic in determining taxonomic species. Therefore the name of
the species can be recognized from the samples acquired. The
results showed that one of the 11 Euplexaura sp. isolates possess
antibacterial activity. Isolates PG-344 possess antibacterial activity
as indicated by the inhibition zone around the isolates. Isolates PG-
344 were able to inhibit the growth of S. aureus with an inhibition
Research Result
zone diameter of 21.86 mm. This is consistent with research done
that revealed that the gorgonian that contain the bioactive
compound can function as an antimicrobial. Gorgonian produces a
metabolism secondary to the environmental conditions in the
crucial competitive interactions within a restricted environment.
Isolates PG-344 have the ability to inhibit the growth of S. aureus
activity caused by the presence of the active compounds.
Staphylococcus aureus has experienced resistance to some
antibiotics, such as chlorampenicol, fosfomicin, tetraciclin and
ciprofloxacine. The clear zone that appears around the isolates
indicates that there are bioactive compounds in isolates which are
capable of inhibiting the growth of S. aureu.
Test bacteria was selected by using a bioassay, which found one
Conclusion isolate, namely PG-344. PG-344 is caple of antibacterial activity
that can protect against methicillin-resistant Staphylococcus aureus
(MRSA). Selection of the test bacteria using a bioassay and gained
1 isolates, namely PG-344 which has antibacterial activity against
methicillin-resistant Staphylococcus aureus (MRSA). Analysis of
the isolate PG-344 showed that 93% of bacteria is closely related
to Virgibacillus salaries.