I.

Tools and Materials

A. Tools
1. Volumetric Flask 10 mL 2 pieces
2. Volumetric Pipette 5 mL 2 pieces
3. Test tube 4 pieces
4. Breaker Glass 100 mL 1 piece
5. Spectrophotometer UV-Vis 1 set
B. Materials
1. Ammonium hydroxide 1 M Sufficiently
2. Cu2+ solution 0,1 M Sufficiently
3. Distilled Water Sufficiently

II. Lanes Work

1. First Solution Test

2 mL of Cu2+ solution 0,1 M

- Add into volumetric flask 10 mL

- Add distilled water until mark point

2 mL of Cu2+ solution 0,1 M

- Observe the absorbance with UV-Vis

spectrophotometer at λ = 700 – 850 nm

Absorbance Value (A)

- If the value A > 1, dilute it until value

of A ≤ 1.

A Value ≤ 1

1
2. Second Solution Test

2 mL of Cu2+ solution 0,1 M

- Add into volumetric flask 10 mL

- Add 5 mL of ammonium 1 M
- Add distilled water until mark point
2 mL of Cu2+ solution 0,1 M

- Observe the absorbance with UV-Vis

spectrophotometer at λ = 350 – 750 nm

Absorbance Value (A)

- If the value A > 1, dilute it until value

of A ≤ 1.

A Value ≤ 1

3. Third Solution Test

2 mL of Cu2+ solution 0,1 M

- Add into volumetric flask 10 mL

- Add 2,5 mL of ammonium 1 M
- Add distilled water until mark point
2 mL of Cu2+ solution 0,1 M

- Observe the absorbance with UV-Vis

spectrophotometer at λ = 350 – 750 nm

Absorbance Value (A)

- If the value A > 1, dilute it until value

of A ≤ 1.

A Value ≤ 1

2
4. Blank Solution

Distilled Water

- Add into volumetric flask 10 mL until

markpoint

Distilled Water

- Observe the absorbance with UV-Vis

spectrophotometer at λ = 400 – 600 nm

Absorbance Value (A)

- If the value A > 1, dilute it until value

of A ≤ 1.

A Value ≤ 1

3
III. Experiment Result
No. Lanes Work
First Solution Test
a. Add Cu2+ 0, 1 M 2 mLinto
volumetric flask 10 mL
b.Add distilled water until
mark point
c. Observe the absorbance
1
with UV-Vis
spectrophotometer at λ =
700 – 850 nm
d.If the value A > 1, dilute it
until value of A ≤ 1.
Second Experiment
a. Add Cu2+ 0, 1 M 2 mLinto
volumetric flask 10 mL
2
b.Add ammonia 1M 5 mL
c. Add distilled water until
mark point
Experiment Result
Hypothesis / Reaction Conclusion
Before After
- CuSO4 : Light blue - CuSO4 dilute : soft Reaction: Based on the
solution light blue solution - 𝐶𝑢2+ + 6𝐻2 𝑂(𝑙) → experiment, we can
- Distilled water : - The absorbance : [𝐶𝑢(𝐻2 𝑂)6 ]2+ (𝑎𝑞) conclude that
colourless 0, 2644 nm wavelength of this
- Wavelength : 811 experiment is 811 nm
nm with absorbance
- 10 Dq : 35,3356 value 0,2644 and
kkal/mol obtained 10 Dq =
35,3356 kkal/mol.
- CuSO4 : Light blue - CuSO4 + NH4OH : Reaction: Based on the
solution dark blue solution - [𝐶𝑢(𝐻2 𝑂)6 ]2+ (𝑎𝑞) + experiment, we can
- Distilled water : (++) 3𝑁𝐻3 (𝑎𝑞) → conclude that
colourless - + distilled water : [𝐶𝑢(𝐻2 𝑂)3 (𝑁𝐻3 )3 ]2+ (𝑎𝑞) wavelength of this
- NH4OH : dark blue solution experiment is 610 nm
colourless with absorbance
4
 Experiment Result
No. Lanes Work Hypothesis / Reaction Conclusion
Before After
d.Observe the absorbance - The absorbance : value 0,4745 and
with UV-Vis 0, 4745 nm obtained 10 Dq =
spectrophotometer at λ = - Wavelength : 610 46,2651 kkal/mol.
350 – 700 nm nm
e. If the value A > 1, dilute it - 10 Dq : 46,2651
until value of A ≤ 1. kkal/mol
Third experiment - CuSO4 : Light blue - CuSO4 + NH4OH : Reaction: Based on the
a. Add Cu2+ 0,1 M 2 mL into solution dark blue solution - [𝐶𝑢(𝐻2 𝑂)6 ]2+ (𝑎𝑞) + experiment, we can
volumetric flask 10 mL - Distilled water : (++) 2𝑁𝐻3 (𝑎𝑞) → conclude that
b.Add ammonia 1M 2,5 mL colourless - + distilled water : [𝐶𝑢(𝐻2 𝑂)4 (𝑁𝐻3 )2 ]2+ (𝑎𝑞) wavelength of this
c. Add distilled water until - NH4OH : dark blue solution experiment is 611 nm
mark point colourless - The absorbance : with absorbance
3
d.Observe the absorbance 0, 2768 nm value 0,2768 and
with UV-Vis - Wavelength : 611 obtained 10 Dq =
spectrophotometer at λ = nm 46,3401 kkal/mol.
350 – 700 nm - 10 Dq : 46,3401
e. If the value A > 1, dilute it kkal/mol
until value of A ≤ 1.

5
 Experiment Result
No. Lanes Work Hypothesis / Reaction Conclusion
Before After
Blank Solution Test - Distilled water : - distilled water : -
e. Add distilled water until colourless colorless
mark point - The absorbance :
f. Observe the absorbance 0, 0000 nm
with UV-Vis - Wavelength : 400
4
spectrophotometer at λ = nm
400 – 600 nm -
g.If the value A > 1, dilute it
until value of A ≤ 1.

6
7