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Food Measure

DOI 10.1007/s11694-017-9663-6

ORIGINAL PAPER

Non-destructive sensing methods for quality assessment of on-tree


fruits: a review
Satyam Srivastava1 · Shashikant Sadistap2 

Received: 9 July 2017 / Accepted: 5 October 2017


© Springer Science+Business Media, LLC 2017

Abstract  Optimum maturity at harvest is a very important application, prediction capabilities, cost, accessibility and
determinant to ensure the final quality of the fruits. Har- reliability.
vesting fruits at appropriate time maintains different quality
parameters such as taste, size, shape, nutritional parameters Keywords  Non-destructive · Harvesting time · Fruits ·
and also have longer shelf life. While as fruits picked too Shelf life · And data fusion
early or too late in season are more susceptible towards dif-
ferent physiological disorders as well as have very shorter
shelf life. So optimal harvesting time judgement is one of Introduction
the most important information for various fruit growers
to optimize the yield and to decrease the on-field losses. India is one of the largest producer of fruits and vegetables
In recent years, various rapid and reliable non-destructive as well as one of the biggest wasters in the world. Money
sensing techniques along with different multivariate, data involved in Indian wastage due to different fruits and veg-
fusion, and chemometric algorithms have been evolved to etables worth Rs 13,300 crores annually. There are various
measure the quality parameters of different fruit samples reasons behind this problem but more than 20% of fruits and
at the time of harvesting. This paper presents an extensive vegetables spoilage occurs due to wrong on-field judgements
review on different on-field issues during harvesting of vari- (Fig. 1) as well as different handling and storage issues [1].
ous fruits and also how these issues significantly contributes One of the most common and important on-field judgement
to fruit spoilage all over the world. Presented paper also that affects the fruits and vegetables quality is optimal har-
consist review on different non-destructive sensing tech- vesting time judgement. Fruits picked at optimal harvest-
niques (electronic nose, spectroscopy, ultrasonic, imaging ing time have better quality parameters compared to fruits
etc) along with various data processing algorithms (data picked too early or too late. Various fruit cultivars can be
treatment, feature extraction, data fusion etc) used for qual- classified in two different classes such as climacteric fruits
ity assessment of different fruit cultivars. Scope of multiple and non-climacteric fruits [2]. Non-climacteric fruits cannot
non-destructive sensing techniques fusion, challenges and continue their ripening cycle after picking from the tree and
bottlenecks also have been explored in the context of fruit also not responds towards ethylene force ripening method.
quality assessment. Various products available in global Climacteric fruit have the capability to continue their ripen-
market are also reviewed based on their sensing technique, ing cycle after harvesting and also towards ethylene force
ripening method. Force ripening practices are not good for
human health as well as it damages different fruit quality
parameters such as taste, nutritional parameters etc [3].
* Satyam Srivastava
So, in the case of non-climacteric fruit samples, optimal
satyamsrivastava@ceeri.res.in
harvesting time is one of the most important parameter at the
1
Academy Scientific & Innovative Research (AcSiR), time of harvesting. One of the other pressing issue related
CSIR-CEERI, Pilani, India to on-field problems is early warning of different disease
2
CSIR-CEERI, Pilani, India attacks and suggestion for right remedial action. Earlier,

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Vol.:(0123456789)
S. Srivastava, S. Sadistap

covers all non-destructive sensing methods in brief along


with the scope of multi-sensing techniques fusion to extract
better information from various fruit samples. Presented
review also discuss about the different on-field problems and
their solution available globally and compares them based
on their features, availability and cost.

Non‑destructive methods for fruit quality


assessment and authentication

Various non-destructive sensing methods have been explored


by different researchers to judge quality parameters of fruits
samples. Few of them have been used for on-tree fruit qual-
ity inspection process, while as others are best suited for lab
level applications. This paper covers six major sensing tech-
niques used over the time period along with their advantages
and disadvantages in the area of fruit identification, quality
inspection and control.

Fruit quality inspection using electronic nose


Fig. 1  Various on-field issues during harvesting of fruits
Sensing technology of artificial olfaction had its beginnings
with the invention of first multi-gas sensor array in 1982
fruit grower was dependent on his own intelligence based [15]. Advancements in aroma sensors technology, electron-
on the change in color, size, shape and weight. Chemical and ics and instrumentation, chemical engineering and artificial
mechanical methods are also used in different fruit prod- intelligence made it possible to develop variety of instru-
ucts industries to accesses the fruit quality [4]. Recently, ments to detect, measure and characterize different vola-
there is a growing interest in different electronic methods tile organic compounds in various applications. Electronic
to accesses the fruit quality parameters. Various electronic noses have been specially designed and developed for the
destructive type methods (impedance measurement, pressure automatic acquisition, detection, identification and classi-
based measurement) [5] have been explored for fruit quality fication of complex mixtures of odours, vapours and gases.
assessment but most of these methods are time consuming, Electronic nose instrument works based on the principle of
damages fruit sample while measurement and best suited human nose (Fig. 3) and it consist of selective and non-
for lab level applications. With the rapid development in selective chemical sensor array for aroma sensing, dynamic
science, technology and computation algorithms, new elec- or static gas sampling scheme for aroma flow control, front
tronic non-destructive sensing methods have been explored end readout interface along with the combination of intel-
to extract different quality parameters of on-tree fruits and ligent pattern recognition algorithms for data acquisition,
also to overcome all existing challenges and bottlenecks processing and quantification.
with destructive methods. Few of these methods such as Various prototypes of artificial e-nose have been
hyperspectral imaging [6], electronic nose (e-nose) [7], designed and developed to discriminate complex odor
ultrasonic based stiffness measurement [8] etc are also used mixture consist of different volatile organic compounds.
in non-contact sensing mode to avoid any kind of damage Most of presented work in the area of e-nose design and
to the on-tree fruit crop. Widely explored non-destructive development uses different kind of sensors such as metal
methods includes UV–Vis–NIR/MIR type spectroscopy oxide (MOS), semi conductive polymer, optical, elec-
sensing, sonic vibration, e-nose, and machine vision and trochemical and surface acoustic wave sensors [16]. In
so on. Sensing methods used for fruit quality measurement most of the applications, cross selective gas or VOC sen-
and authentication over the time period are presented in the sor array has been used to sense wide range of chemical
form of timeline (Fig. 2). Various researchers contributed odor mixture and further to discriminate various diverse
their investigation regarding non-destructive methods for mixtures of possible analytes. Response of individual sen-
fruit quality inspection and authentication but most of them sors are collectively integrated to produce a unique digital
were focused on lab level solutions and haven’t discussed the response pattern and further converted in to the form of
on field problems and their solutions. Presented investigation unique aroma signature. Collected signatures represents

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Non-destructive sensing methods for quality assessment of on-tree fruits: a review

Fig. 2  Sensing methods used for fruit quality assessment and authentication [9–14]

Table 1  Different volatile organic compounds found in different fruit


cultivars

Fruit cultivar Chemical family Major compound Ref.

Apple Aliphatic esters Ethyl butanoate [18]


Banana Aliphatic esters Isoamyl acetate [19]
Peach Lactones γ-Decalactone [20]
Kiwifruit Aliphatic aldehydes Hexanal [21]
Pineapple Organic acids Hexanoic acid [22]
Raspberry Aliphatic ketones Raspberry ketone [23]
Fig.  3  a Functional diagram of human odor sensing scheme, b Strawberry Furanone lactones Furaneol [24]
mimic diagram of electronic nose Tomato Apocarotenoids β-ionone [25]

as the identity of a simple or complex mixture, which


could be further utilized for training, testing and clas- Kiani et  al. presented a new portable e-nose system
sification. Fruit aroma consist of a complex mixture of (Fig. 4) for identification of different types of saffron, stigma
different VOCs whose composition corresponds to spe- of Crocus sativus L. (Iridaceae), based on their volatile
cific plant and fruit cultivar. Few of the fruits share many organic compounds (VOC’s). Presented e-nose uses metal
aromatic characteristics while as each fruit also have a oxide based gas sensor array consist of ten gas sensors, tem-
distinctive aroma that depends upon the combination of perature, humidity sensor along with direct head space sam-
volatile organic compounds [17]. Most common aroma pling integrated with microcontroller based real time data
compounds found in different fruit cultivars (Table 1) are acquisition unit. Eleven saffron samples have been collected
phenols, esters, ketones, aldehydes etc. from different regions for analysis and testing. Authors used

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S. Srivastava, S. Sadistap

Fig.  4  a The portable e-nose expert system developed for saffron aroma quality evaluation. b The sensor array and the test chamber. Repro-
duced with permission from Kiani et al. [26]. Copyright Elsevier 2016

principal component analysis (PCA) (Fig. 5) and hierarchi- samples. This was further confirmed by HCA which classi-
cal clustering analysis (HCA) as unsupervised models and fied the groups into five distinct quality classes (excellent,
multilayer perception (MLP) neural networks and partial very good, good, medium and poor quality) which were used
least squares (PLS) as supervised models were utilized to as the MLP and PLS classification goals. Results of analysis
develop the e-nose classification capability. Based on the showed that performance of the MLP model for prediction
results, PCA of volatile compounds fingerprints revealed of saffron samples quality class was better than PLS model,
11 distinct groups corresponding to 11 different saffron with 100% success rate and high correlation coefficients of

Fig. 5  PCA score plot of the


characterization of 11 different
saffron samples with various
aromas [26]. Reproduced with
permission from Zhang et al.
[27]. Copyright Taylor and
Francis 2016

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Non-destructive sensing methods for quality assessment of on-tree fruits: a review

cross validation (­ R2 = 0.989 and relatively low RMSE value at three different storage conditions and the volatile pro-
of 0.141) [26]. Zhang et al. presented a case study to analyse file changes were followed subsequently over a period of
the soluble solid content in persimmon fruits using com- 15 days. PCA has been implemented on collected e-nose
mercial available e-nose system (PEN3). Presented system data and score plot of PCA showed no storage history effect
consist of sampling apparatus, a detector unit containing an and only very little shelf life effect. While as MSE-Nose and
array of ten different metal oxide sensors and pattern recog- GC/MS score plots clearly indicated the presence of both
nition software (Win Muster v. 1.6) for data acquisition and shelf life and storage history trend. Moreover, the volatile
analysis. PCA has been used to discriminate different pick- profile changes during shelf life depended on the storage
ing dates for persimmon samples corresponding to different history. Partial least mean square model has been imple-
maturity level (Fig. 5). While as authors also used support mented to correlate the firmness with days of shelf life with
vector machine (SVM), multiple linear regression and back the volatile production of apples [29].
propagation neural network and it has been observed that Brezmes developed based e-nose to measure the shelf
SVM shows better prediction accuracy [27]. life of pink lady apples based on the change in the ripe-
Zhang et al. presented a study to predict optimal harvest- ness stage. In this study, a personal computer e-nose sys-
ing time for peach samples based on the responses of the tem has been developed to accesses the ripeness state of
metal oxide based gas sensor array. Authors implemented apples throughout their shelf life. Authors used PCA and
PCR and PLS model on collected gas sensor array data and fuzzy ART technique for classification between mature and
it has been observed that PLS model represents a good abil- immature apple samples. PCA analysis doesn’t shows any
ity in predicting quality index, with high correlation coef- clustering behaviour that might be attributed to ripening
ficient ­(R2 = 0.86) for penetrating force, sugar content and while as fuzzy ART shows a tendency to classify measure-
acidity (Fig. 6) [28]. ments regarding to their ripeness stage (Fig. 7) [30].
Saevels presented a study to understand the potential Llobet et al. developed an e-nose using an array inex-
of the e-nose along with mass spectrometer based e-nose pensive commercial tin oxide odor sensors and used them
(MSE-Nose) to monitor the changes in apple fruit volatiles to analyse the ripeness states of banana samples. Authors
during shelf life. Collected apples were stored for 8 months used PCA along with investigatory techniques to classify

Fig. 6  Scatter plot of the first two main axes illustrating the e-nose sensor response data categorized into the three groups: a PCA and b LDA.
Reproduced with permission from Zhang et al. [28]. Copyright Springer 2012

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S. Srivastava, S. Sadistap

Fig. 7  PCA scores using col-


lected e-nose signal a PCA are
computed using fruit and cali-
bration measurement togther, b
PCA are computed using fruit
measurement alone then both
calibration and measurement
projected. Reproduced with
permission from Brezmes [30].
Copyright Elsevier 2004

banana ripeness stages based on the change in sample skin to predict storage life based on the change in volatile pro-
color. Three other supervised classification techniques duction of mandarin samples. Authors used commercial
such as fuzzy ARTMAP, LVQ and MLP also have been e-nose (PEN-2) technology for measurement and testing
used to classify the samples into the observed seven states purpose. PCA and LDA have been used to classify man-
of ripeness and it has been observed that fuzzy ARTMAP darin samples having different shelf life, LDA shows good
and LVQ classifiers outperformed the MLP classifier with classification results and well classified mandarin samples
the accuracies of 90.3 and 92% compared with 83.4% having same storage time [32]. Lebrun et al. used e-nose
respectively [31]. Gomez et al. used e-nose technology

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Non-destructive sensing methods for quality assessment of on-tree fruits: a review

along gas chromatography technology to predict matu- fruit quality inspection have been summarized in tabular
rity stages of mango samples. Harvested mango samples form as following (Table 2).
have been evaluated using GC and E-Nose (Alpha FOX
3000) for aroma and other volatiles as well as for soluble Fruit quality inspection using UV–Vis–NIR
solid and acids. Volatile data from the different harvest spectroscopy
maturities and ripening stages were discriminated using
multivariate statistics (DFA). Both the e-nose and GC Fruits have different absorption and reflection properties
results were able to separate fruits having different har- in the light of different wavelength band change in their
vest maturity index [33]. Yu et al. investigated about long internal attributes such as sugar, acidity, SCC etc. Electro-
Jing green tea quality parameters using electronic nose magnetic spectrum covers a large range of photon energies
technology. Commercially developed PEN-2 electronic which could be divide in to different bands such radio wave,
nose instrument to measure the quality parameters of long microwave, infrared, near infrared, visible, ultraviolet, X-ray
Jing green tea samples. Four different groups based on dif- etc. Optical properties indicate the response of the fruits to
ferent quality grade (A120, A280, A380 and A600) have visible light band (400–700 nm) and near infrared (NIR)
been used for measurement, training and testing proce- band (700–2500 nm). When a light beam falls on an object,
dure [34]. Xiaobo et al. presented an e-nose based method part of the incident beam gets reflected by the surface of the
to classify apples based on their maturity and freshness. object, which is called as regular reflectance. Other remain-
50 fresh and 50 rotten apples have been collected and ing radiation is transmitted through the surface into cellular
used commercial e-nose setup. PCA and GA-RBF neu- structure of the object where it gets scattered by small inter-
ral network based algorithms have been used for feature faces with in the tissues or absorbed by cellular constituents.
extraction process from measured dataset. Satisfactory Absorbed portion of radiation could be transformed to dif-
classification has been shown between fresh and rotten ferent parts of energy such as heat, luminescence, chemical
apples and it has been observed that GA-RBF has been changes etc. So, overall part of the transmitted energy gets
shown 96.4% recognition probability for fresh and rotten absorbed (absorption) and some part is reflected back to
apples [35]. Wilson et al. reviewed various advancements the surface and remaining part transmitted through object
in the area of e-nose development along with their applica- (Fig. 8) [56].
tions in different area such as food, medical etc. Authors Visible and NIR spectrum majorly explored for fruit
explained different types of aroma along with its role in internal quality inspection while as ultraviolet (UV) band
human society and commerce. Concept of different types used for disinfection of fruits and beverages from differ-
of e-nose instrumentation along with various data treat- ent bacteria’s, virus etc. Visible and NIR spectroscopy have
ment and analysis techniques also have been explained in been utilized for fruit quality measurement for many years.
detailed [36]. Falasconi et al. reviewed e-nose technology NIR spectroscopy has several advantages over the visible
for microbiological screening of various food products. spectroscopy such as NIR radiation is highly penetrative and
Authors presented a detailed review of four case studies thus it can be applied directly to the sample without any
for microbial contamination detection using e-nose: (1) sample preparation. Other advantages are that they could
Alicyclobacillus spp. spoilage of fruit juices, (2) early provide quantitative information on the major organic com-
detection of microbial contamination in processed toma- ponents of food products. Various modes have been explored
toes, (3) screening of fungal and fumonisin contamination for different category of fruits like transmission mode is con-
of maize grains, and (4) fungal contamination on green sidered to access sugar, SSC and acidity of thick skinned
coffee beans. Baietto et al. reviewed the chemical nature fruits [57]. Few of the other explored modes are reflectance
of different fruit volatiles during different ripeness stages and interactance (Fig. 9). In reflectance method, source and
of the agro-fruit production process and describe some of detector lactated in the same direction and when fruit get
the important applications for fruit identifications, cultivar illuminated by source, scattered reflected radiation recorded
discriminations, ripeness assessments and fruit grading for by the detector. Generally different lamps are used as a
assuring fruit quality in commercial markets [37]. Ampu- source and diode array as detector while as few of efforts
ero investigated the efforts have been taken in the direction also have been taken in the direction of LED array as source.
e-nose application for dairy products quality inspection Cen et al. reviewed the potential of NIR reflectance spec-
such as milk, cheese etc. Authors explained different types troscopy to determine the quality of various food samples.
of sensors technology (nose metal oxide, conductive poly- Authors explained the concept and features associated with
mer etc) used in electronic along with different data treat- NIR reflectance spectroscopy along with different issues
ment methods (PCA, LDA, PLS, DFA etc) [38]. Few of and challenges related to spectroscopy system design. Pre-
the other major efforts taken in the direction of e-nose for processing and chemometric techniques used for qualitative
and quantitative used for calibration model development

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Table 2  Major contributions in the area of electronic nose for fruit quality assessment and authentication
Fruit cultivar Sensing technique Instrumentation Parameters Preprocess Data processing Validation Model accuracy Ref.
details details

13
Apple (Royal Gala) Electronic nose + GC FOX 4000 (Alpha Maturity, Harvest Residual maximum PCA, DFA Cross-validation by – [39]
MOS System) date likelihood (REML), GC data
Descriptive Profil- least significant dif-
ing by 18 trained ference (LSD)
experts about
aroma, flavor,
texture
Apples (Pinklady) Electronic nose Customized experi- Shelf life, firmness, Normalization PCA, Fuzzy ART – Firmness: 0.93 [30]
mental setup [21 starch, pH Starch: 0.68
sensors (FIS(SB/SP pH: 0.84
Series), TGS)]
Banana Electronic nose PC based electronic Ripeness stage Normalization PCA, LDA, SIMCA, – Classification: [40]
nose experimental SVM 98.66%
setup consist of 6
gas sensors along
with dynamic
sampling scheme,
NI-USB6009
Mango (Harumanis) Electronic nose Developed electronic Ripeness stage Normalization PCA, ANN (MLP) – Classification: 95% [41]
nose experimental
setup with 8 metal
oxide gas sen-
sors, software on
Labview
Strawberry Electronic E-nose (PEN 3, Win Pathogenic fungal Normalization PCA, ANOVA, MLP Cross-validation by Classification: 96.6% [42]
nose + GCMS Muster Air-sense disease detection GCMS
Analytics Inc., (Botrytis, Penicil-
Germany) lium and Rhizopus)
Peach Electronic nose + GC Electronic nose (PEN Ripeness stage Normalization PCA, LDA, CART Cross-validation by Recognition rate: [43]
2, Win Muster Air GC 100%
sense)
Apples (Malus Electronic nose Libra Nose (Tech- Soluble solids, Normalization, drift PCA, PLS – SCC: 0.77 Firmness: [44]
domestica Borkh.) nobiochip, Elba acidity, starch and compensation 0.80 Acidity: 0.69
Island, Italy) firmness
Apples (Romanian) Electronic nose Alpha FOX 3000 Acidity, soluble Normalization PCA, DFA – Recognition Per: [45]
solid, sweetness, 100%
astringency
Peaches and Nectar- Electronic nose Libra nose (Tech- Sweetness, flesh Relative normaliza- PCA, LVQ – Identification: 86% [46]
ines nobiochip, Elba color tion and average
Island, Italy)
S. Srivastava, S. Sadistap
Table 2  (continued)
Fruit cultivar Sensing technique Instrumentation Parameters Preprocess Data processing Validation Model accuracy Ref.
details details

Mandarin Electronic nose Electronic nose (PEN Picking dates (ripe- Normalization PCA, LDA – Classification rate: [47]
2, Win Muster Air ness stage) 92%
sense)
Peaches, nectarines, Electronic nose Customized experi- Firmness, saturation, Normalization PCA, PLS – Firmness: 0.94, satu- [48]
apples, and pears mental setup of PC hue, firmness, SCC ration: 0.79, hue:
based electronic 0.99, SCC: 0.92
nose (dynamic sam-
pling scheme with
24 MOS Sensors)
Peach Electronic nose Customized experi- Penetrating force, Normalization MLR, QPST and – Penetrating force: [49]
mental electronic acidity, sugar BPANN 0.87
nose with MOS content Acidity: 0.81
sensors Sugar content: 0.79
Peach and pears Electronic nose Customized experi- Fruit ripeness Relative normaliza- PLA, MLP – Success rate: 92% [50]
mental PC based tion
electronic nose with
MOS sensors
Apple (Fuzi) Electronic Nose Customized experi- Storage time Normalization, sto- PCA – Success rate: 84.62% [51]
mental PC based chastic resonance
electronic nose with signal-to-noise ratio
MOS sensors
Peaches Electronic Nose e-nose EOS 835 Sweetness, juiciness, ANOVA, Student– PCA Sweetness: 0.92 Juci- [52]
Non-destructive sensing methods for quality assessment of on-tree fruits: a review

(SACMI, Imola, texture Newman–Keuls ness: 0.92, Texture:


Italy) separation 0.93
Banana Electronic nose Customized experi- Ripeness stage Normalization Fuzzy ARTMAP, – Success rate: 92% [31]
mental PC based LVQ and MLP
electronic nose with
MOS sensors
Apple Electronic nose Cyranose 320 (Model Firmness, TSS, starch Normalization PCA, MANOVA, – Success rate: 83% [53]
320, Cyrano Sci- DA, CCA
ences)
Pear Electronic nose Customized Electron- Acidity, SCC, firm- Normalization PCA, PCR, LDA, – Acidity: 0.56 [54]
ics with six MOS ness MLR, PLS SCC: 0.94
sensors along with Firmness: 0.92
USB-6009 card
Oranges and Apples Electronic nose Libra nose (Tech- Mealiness, skin Normalization PCA, PLS, PLS-DA Cross-validation Success rate: 99% [55]
nobiochip, Elba damage
Island, Italy)

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S. Srivastava, S. Sadistap

Ref.

[27]
Model accuracy

SCC: 91.36%
Cross-validation

Fig. 8  Light distribution scheme on fruit sample


Validation

PCA, MLR, SVM


Data processing

and BPANN
Normalization
Preprocess

Picking dates (SCC)


Parameters

E-nose (PEN 3, Win


Muster Air-sense
Analytics Inc.,
Instrumentation

Germany)
details
Sensing technique

Electronic nose

Fig. 9  Different geometrical configurations a reflectance mode, b


transmittance mode
details

are also reviewed and explained in detail [58]. Jha et al.


reviewed different non-destructive techniques contributed
Table 2  (continued)

by various researchers for quality evaluation of intact fruits


and vegetables. Nuclear magnetic resonance (NMR), X-ray,
Fruit cultivar

Persimmon

computerized tomography and spectroscopy techniques


have been reviewed and explained in detail. Scope of dif-
ferent miscellaneous techniques such as acoustic based

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Non-destructive sensing methods for quality assessment of on-tree fruits: a review

firmness detection, color detection using machine vision etc in to two different classes based on sugar content and the
also has been explored [59]. Magwaza et al. talked about the overall accuracy of classification is around 91% [61].
application of NIR spectroscopy for internal and external Huang et al. reviewed application of NIR spectroscopy
quality analysis of citrus fruits. Authors explained the con- for online monitoring of quality in food and beverage sam-
cept of NIR spectroscopy, wavelength band selection, data ples such as such as meat, fruit, grain, dairy products etc.
acquisition positions, spectral preprocessing and chemo- Presented review emphasize the methods designed for on/in-
metric models specially used for various citrus cultivars. line measurement of data, chemometric treatment, as well as
Challenges with model accuracy and robustness also have interpretation of the experimental observations [62]. Lam-
been discussed in detail [60]. Choi et al. reviewed various mertyn et al. investigated about the light penetration proper-
efforts taken in the direction of NIR spectroscopy for fruit ties of NIR radiation for apples. Authors explored bifurcated
quality evaluation along with different measurement modes and optical configuration for spectroscopy data acquisition
such as half and full transmission and reflectance mode. over the wavelength band of 500–1900 nm. Partial least
Multiple linear regression model (MLR) has been devel- mean square (PLS) model has been used on collected spec-
oped using 16 wavelength data for sugar content estimation troscopy data for calibration model development. Results
of peach samples with determination coefficient of 0.71 and shown that bifurcated configuration (­ R2 = 0.91) performed
standard error of calibration of 0.42 Brix. Model. Overall better compared to optical configuration (­ R2 = 0.81) for SSC
developed system (Fig. 10) could classify peach samples prediction of apple samples [63] (Fig. 11).
Peirs et al. used visible and NIR spectroscopy for opti-
mal harvesting date’s prediction of different apple cul-
tivars. Authors used commercial spectrometer (Optical
Spectrum Analyzer (OSA) 6602, Rees Instruments Ltd.,
Goldalming, UK) having range of 380–2000 nm with the
resolution of 0.5 nm. Authors used multiplicative scatter

Fig.  10  a Peach quality grading system, b calibration model using Fig.  11  a The bifurcated optical configuration, b The 0°:45° optical
PLSR. Reproduced with permission from Choi et al. [61]. Copyright configuration. Reproduced with permission from Huang et  al. [63].
ISEECAF, 2006 Copyright Elsevier 2000

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S. Srivastava, S. Sadistap

correction along with PCA for feature extraction and Few of the other major efforts taken in the direction of
PLS for calibration model development. Presented study spectroscopy technique used for fruit quality inspection have
predicted SSC, acidity, firmness and maturity of apple been summarized as following (Table 3).
samples with good correlation coefficient [64]. Das et al.
recently presented a smartphone based spectrometer for Fruit quality inspection using ultrasound sensing
instantons ripeness detection of apple samples. Devel- technique
oped system measures apples properties over the wave-
length band of 340–780 nm with the resolution of 15 nm. Ultrasonic sensing technology is popularly used various food
Authors basically tried to measure the chlorophyll con- quality monitoring and control applications such as emulsifica-
tent in apple samples and further converted in to ripeness tion, freezing, thermosonication, cavitation and disinfection.
stage. Authors developed 5th order calibration model to Recently, there is a growing interest for ultrasound technique
predict chlorophyll content of apples. Overall presented application for non-destructive food quality measurement.
system is very portable and have satisfactory accuracy of Ultrasound technique could be used directly on field or in
61–74% [65] (Fig. 12). industries for food quality inspection due to its non-destructive

Fig.  12  a Close-up of the nozzle depicting illumination and collection geometry, b interface for spectral acquisition with integration times and
modes of operation, c typical ChlF for Golden Delicious apple sample. Reproduced with permission from Das et al. [65]. Copyright Nature 2016

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Table 3  Major contribution in the area of UV–Vis–NIR spectroscopy for fruit quality assessment and authentication
Fruit cultivar Sensing technique Instrumentation Parameters Preprocess Data processing Model accuracy Error analysis Ref.
details details

kiwifruit (Actinidia Visible–NIR spec- Commercial SCC, density, internal Scaling, smooth- PLS and PCR cali- SCC: 0.93, density: SEP SSC: 0.80 [66]
chinensis) troscopy spectrom- flesh color ing, absorbance bration models 0.74, internal flesh brix, Density:
eter wavelengths: transformation, color: 0.82 3.6 kg m−3, Internal
300–1100 nm with MSC, SNV, NSD, Flesh Color: 1.6
3.3 nm res ABSD1-2
Apples NIR diffuse reflec- Commercial spec- Firmness, sugar Relative reflectance, PCA for features Firmness 0.38 0.58, SSC: 0.37–0.42 brix, [67]
tance spectroscopy trometer wave- content absorbance calcula- extraction and PCR SCC: 0.93–0.97 Firmness: 6.6-
lengths 300 to tion, smoothing, 10.1N
1100 nm with 2 nm MSC
res
Mango NIR reflectance PC based experimen- Firmness, total solu- First and second MLR, PCA and PLS Firmness: 0.9276 Firmness 1.223 [68]
spectroscopy tal setup with com- ble content, acidity, derivative of reflec- TSS: 0.6085 TSS: 0.161
mercial spectrom- storage period tance spectrum Acidity: 0.8226 Acidity: 0.8226
eter Wavelength: Storage period: Storage period:
1200–2400 nm with 0.9380 0.9380
1 nm res
Blueberries MIR and NIR spec- Instrument: FT–NIR Ripening Parameters Mean, SD, and mul- Partial least squares TSS: 0.96 TSS: 0.65 [69]
troscopy spectrometer (MPA, (TSS), nutraceuti- tiplicative scatter (PLS) regression Phenols: 0.87–0.97 Phenols: 0.18
Bruker Optics, cal compounds correction algorithms Flavonoids: 0.97 Flavonoids: 0.25
Ettlingen, Ger- (phenols, total Ascorbic acid: 0.91 Ascorbic: 0.22
many) Wavelength: flavonoids and Anthocyanins: 0.92 Anthocyanins: 0.80
833–2777 nm total anthocyanins,
Non-destructive sensing methods for quality assessment of on-tree fruits: a review

ascorbate)
Golden Delicious Hyperspectral imag- A hyperspectral Flesh color, firmness, Normalization, SD, PCA for feature Acidity: 0.57 Acidity: 0.8 [70]
Apple ing fluorescence imag- soluble solids con- mean and max extraction and SCC: 0.66 SSC: 1.19
ing system has been tent, and titratable ANN for training Flesh color: 0.93 Flesh color: 0.03
developed Wave- acid Firmness: 0.75 Firmness: 8.57
length: 408–430 nm
Apple parenchyma, Visible–NIR spec- Bifurcated and opti- Soluble solids content Normalization, SD, PLS model has been Bifurcated: 0.91 bifurcated_ [63]
Jonagold troscopy cal config. has been (SCC) mean and max used to develop Optical conf.: 0.87 RMSEP:0.73 Opti-
used to study the calibration model cal_RMSEP: 0.87
penetration depth of
Vis/NIR spec-
trum, Wavelength:
500–1900 nm
Apricot (Kioto and Visible–NIR spec- Portable spectrometer Soluble solids content SNV, second deriva- FDA and PLS SSC: 0.88–.96 SSC: 0.67–1.1 TA: [71]
Harostar) troscopy has been devel- (SSC), total acidity tive after SNV TA: 0.73–0.97 24
oped using off (TA) and firmness Firmness (Fi): 0.92 Firmness (Fi): 6.2–13
the shelf source, (Fi)
detector, grating,
filters Wavelength:
650–1200 nm

13

Table 3  (continued)
Fruit cultivar Sensing technique Instrumentation Parameters Preprocess Data processing Model accuracy Error analysis Ref.
details details

13
Satsuma mandarin Vis–NIR spectros- Commercial spec- Firmness soluble sol- Averaging, Smooth- PCA, PCAR and PLS Firmness: 0.83 Firmness: 8.53 [72]
copy trometer system has ids content (SSC) ing, MSC SCC: 0.94 SSC: 0.33
been used having acidity Acidity: 0.8 Acidity: 0.18
wavelength range of
35–2500 nm
Mango fruit NIR spectroscopy Commercial spec- SCC, DM, Starch MSC MLR, PLS DM : 0.86 DM: 0.73 [73]
trometer Wave- Starch: 0.82 Starch: 0.77
length: 700–
1100 nm
Apple Vis–NIR spectros- Commercial spec- Soluble solids, Streif Normalization, PCR, PLS Maturity: 0.90 Maturity: 7.4 [64]
copy trometer Wave- index, acidity and second derivative, Streif index: 0.84 Streif index: 0.18
length: 380– firmness, maturity MSC SCC: 0.84 SCC: 0.73
2000 nm Acidity: 0.80 Firmness: 1.13
Firmness: 0.78
Apricot FT-near-infrared Commercial spec- Soluble solids and Normalization, SNV PCA, PLS Soluble solids: 0.92 Soluble solids: 0.98 [74]
spectroscopy trometer Wave- titratable acidity Titratable acidity: Titratable acidity:
length: 800– 0.89 3.62
2500 nm
Mandarin NIR spectroscopy Experiment setup Light distribution Monte Carlo simula- – – – [75]
developed (laser study tion of the light
diode: 808 nm) levels inside a
mandarin
Apple (Malus domes- UV–Vis spectroscopy Developed smart Ripeness stage chlo- Averaged, noise 5th order calibration Firmness: 0.61–0.74 – [65]
tica) phone based spec- rophyll maturity removal model
trometer wave-
length: 340–780 nm
with 15 nm
S. Srivastava, S. Sadistap
Non-destructive sensing methods for quality assessment of on-tree fruits: a review

nature. Other advantages of ultrasound sensing technique over Generally, ultrasound based measurement can be cat-
other non-destructive techniques are that it is highly sensitive, egorized into two types such as contact and non-contact
has fast response, is robust and is able to perform high velocity type mode. Contact type ultrasound measurement method
measurement. Ultrasound technique is performed by accessing has been explored by several researchers for different food
the echo wave propagated through different mediums. Ampli- and beverage materials while as non-contact measurement
tude and time of flight of the propagated echo signal changes method still have its own challenges and limitations. Three
with change in the properties of the material (refractive index, types of ultrasonic sensing modes have been explored by
weight, mass etc). Furthermore, recorded amplitude and time different researchers such as pulse echo, pitch catch and
of flight of echo ultrasound signal could be converted in to transmission to measure the thickness, velocity, density
attenuation and propagation delay introduced due to test sam- and defects of the test sample. In transmission mode, test
ple [76]. General architecture of a ultrasound measurement sample is located between the transmitter and receiver and
experimental setup consist of a pulse triggering circuit having evaluation of the test sample is based on transmission wave.
frequency of ultrasound transmitter centre frequency, band Besides pitch catch mode is based on the reflection wave
pass filter having, zero threshold detector unit, time to digital where transmitter and receiver installed at the same side.
converter unit and processor unit to calculate attenuation and Transmitter and receiver transducer can be couple on the
propagation delay in echo signal (Fig. 13). single chip and work as a transceiver. Pulse echo model used
the transceiver where the test sample was evaluated based on
Tpd = Tstop − Tstart
change in reflection wave (Fig. 14a, b, c). Selection of ultra-
Attenuation = Atransmitted − Arecieved sonic sensing mode depends on the nature of application as
well as frequency range of transmitter and receiver like for
Attenuation = 20log10 Attenuation potato stiffness assessment pitch catch or pulse echo mode
will be better compared to transmission mode because ultra-
sonic transmitted signal cannot propagate through potato
flesh [78].
Frequency level for producing acoustic signal depends
on the nature of application such as safety, quality assur-
ance, product modification or others. Low frequency acous-
tic signal (20–100 kHz) consist higher power and could be
used for quality inspection of fruits having thick peel while
as other low frequency applications in food industries are
fermentation, drying, emulsification and crystallization.
High frequency ultrasonic signal (2–19 MHz) consist low
power could be useful for food quality assessment [77]. A
very first effort in the direction of ultrasonic based quality
assessment has been taken by Ha et al. for potato quality
inspection. Authors used longitudinal ultrasonic transducer
with the frequency range of 50 kHz–1 MHz and change in
the time of flight and amplitude has been measured for rotten
and healthy potato samples. It has been observed that change
in ultrasound signal amplitude and time of flight propa-
gated through potato flesh can be easily classified between
healthy and rotten potatoes [78]. Mizrach et al. presented
a similar kind of ultrasound sensing experimental setup
for ripening detection of autumn and winter grown ‘galia’

Fig.  13  a General architecture of ultrasonic measurement experi-


mental setup. b Yellow: triggering TX pulse train. Violet: echo ultra- Fig. 14  Ultrasonic sensing mode a transmission; b pitch catch; c
sound signal. Cyan: ultrasound echo signal converted in to pulse form pulse-echo (T transmitter, R receiver, TR transceiver)

13
S. Srivastava, S. Sadistap

melons. Firmness, dry weight and total soluble solid have General computer vision experimental setup consist of a
been measured using commercial mechanical ultrasound camera to capture the image of the sample, light source with
sensing experimental setup (Chatillon durometer) and good directional waveguide for proper illumination, color filters
correlation model ­(R2 = 0.80) has been found [79]. Mizrach and connectivity with a personal computer or an embedded
presented a non-destructive ultrasonic method to monitor the system for image acquisition, storage and processing. Accu-
firmness and sugar content of greenhouse tomatoes during racy of an application for fruit quality is highly dependent on
shelf life. Author measured the acoustic wave attenuation various factors such as quality of the input image, illumina-
by using contact type method and a second order non-linear tion conditions, and orientation of camera, shape and size
model with good correlation index ­(R2 = 0.9648) has been of the object to be inspected [98]. To develop an automated
found between attenuation of the reflected ultrasound signal machine vision sensing system for fruit quality inspection,
and the firmness of the tomato samples while as unable to image process techniques are often combined with mechani-
correlate the firmness results with sugar index [80]. Saggin cal and instrumental devices to minimize the human inter-
et al. measured the density of sucrose (0–60 wt%), glyc- vention involved. Machine vision based system has been uti-
erol (0–50 wt%), sodium chloride (0–27 wt%) solutions and lized at various places such as shorting and grading based
tomato ketchup (0–100 wt%) using a modified ultrasound on size, shape, color and defects on the outer surface of the
experimental setup. Authors used pulse echo technique fruits, counting the number of ripen fruits on tree, disease
with the ultrasound transducer having a centre frequency detection and recognition etc [99]. Aleixos et al. presented
of 2.25 MHz. Linear model with high correlation index a multispectral based real time inspection for different citrus
­(R2 = 0.999 for sodium chloride, tomato ketchup, glycerol cultivars using machine vision and digital signal processors.
and ­R2 = 0.994 for sucrose) has been obtained between the Presented work includes the development of a multispectral
density variations and the amplitude of the echo signal [81]. camera, which is able to visible and NIR images from same
Gaete-Garretón contributed a novel non-invasive ultrasonic scene. Authors also designed application specific algorithms
based method for avocado ripening prediction. Results and their implementation on a specific board having two
were validated with the standard penetrometer results and a parallel DSPs to divide the inspection task and to reduce
high correlation index ­(R2 = 0.9762) linear model has been the overall processing time. Developed system is able to
obtained between acoustic absorption coefficient of propa- inspect different cultivars of citrus based on size, color and
gated ultrasound signal and firmness of the avocado sam- presence of defects at a minimum rate of 5 fruits/s [100]. Du
ples [82]. Dolatowski et al. reviewed various applications of et al. reviewed various learning techniques used in computer
ultrasound for inactivation process of different bacteria and vision for food quality inspection in recent years such as sta-
meat quality assessment [83]. Mizrach reviewed ultrasonic tistical learning, fuzzy logic, genetic algorithms and decision
technology applications for fruits and vegetable assessment tree [101]. Brosnan et al. reviewed various advancement in
during pre and post harvesting process. Author explained the area of computer vision for quality inspection of food
fundamentals of ultrasound technology and associated qual- products. Authors explained the fundamentals of computer
ity parameters for fruits and vegetables [84]. Few of the vision with their various food quality applications such as
other major efforts taken in this area have been summarized bakery products, meat, fish, fruits and vegetables [102]. Few
as following (Table 4). of the other major efforts taken in this area have been sum-
marized as following (Table 5).
Fruit quality inspection using machine vision sensing
technique
Scope of multiple non‑destructive sensing
The application of machine vision in the area of food indus- techniques fusion
try has increased considerably in recent years. The quality of
a processed or fresh fruits or vegetable could be defined by Recently, there is a growing interest towards multiple sens-
a series of physicochemical parameters such as weight, size, ing techniques fusion for improving the quality assessment
shape, colour ripeness etc. Most of these factors have tradi- and authentication of various food samples. Fusion of data
tionally been accessed by visual inspection by trained opera- from complementary sensing techniques can provide bet-
tors but this is time consuming, required more man power ter insight about any food sample than a single sensing
and always there is a high risk involved due to human error. technique. The concept of multiple sensing techniques
Automated computer vision has been taken in to account to fusion is inspired by humans, just like human mind relies
decrease the human intervention involved and at the same on multiple sensing techniques in order to achieve more
time to increase the accuracy. Automated computer vision accurate inferences about food suitability and improve
can simplify different tedious monitoring processes that take the chance of survival. Data fusion approaches have been
a long time and required complex apparatus to performed combined in different ways to improve the classification

13
Table 4  Major contribution in the area of ultrasound based measurement for fruit quality assessment and authentication
Fruit Cultivar Sensing technique details Instrumentation details Parameters Preprocess Data processing Model Accuracy Reference

Plum Nondestructive ultra- High-power, low- The acoustic pulse ampli- Acoustic wave attenua- Firmness linear model- R2 = 0.8996 [85]
sound method was used frequency ultrasonic tude were recorded tion, analyzed ling attenuation
as sensing technique pulser-receiver (Kraut- until full ripening was
kramer, Model USL33), detected. Firmness,
a pair of 50-kHz ultra- attenuation
sonic transducers
Apple Ultrasonic energy absorb- Chatillon LRX testing Mealiness in apples Energy absorbance cal- Energy profile R2 = 0.69 [86]
ance machine and cylindrical culation
probs
Avocado fruits Ultrasonic technique Krautkramer USL 33 Transit time of the acous- Acoustical parameters Correlation, curve fitting R2 = 0.9946 [87]
high- power low- tical wave and the pulse analysis
frequency ultrasonic amplitude, Firmness,
pulser/receiver, a pair refractive index in oil
of 50-kHz narrow-band content, dry weight
ultrasonic transducers
Apple, Pear, and Peach Ultrasonic measurement High power ultrasonic Attenuation, velocity and Elastic modulus, bio Multi-linear regression Apple: 0.706 [88]
techniques pulser (Ritec Model peak frequency yield strength, force– analyses, statistical Pear: 0.861
SP-801) and a broadband deformation curves, regression analysis Peach: 0.675
receiver (Ritec Model Multi-linear regression
BR-640). Panametrics, models
peak frequency: 0.5
[MHz]
Korean Apple Ultrasonic measurement UTM (Universal testing Apparent elastic modulus, Ultrasonic velocity Linear regression model R2 = 0.833 [89]
Non-destructive sensing methods for quality assessment of on-tree fruits: a review

techniques machine) ultrasonic velocity, measurement, firmness


apparent attenuation measurement
Avocado fruits Ultrasonic effect Acoustic generator (pie- Surface acoustic absorp- Theoretical calculations, Normalization of ripen- – [82]
zoelectric pre-stressed tion coefficient, equato- absorption, firmness ing state
sandwich transducer rial diameter, weight, and error analysis at dif-
coupled to a stepped and firmness ferent interval of time
horn made of titanium
alloy)
Orange Ultrasonic eco pulse Panametrics PI20-2 20; Velocity measurement Signals backscattered Longitudinal speed com- – [90]
method 0.25 F = 2.00 0690004,, Minimization pression, peel thickness
Pulser-Receiver model compression
5052PR Sofranel,,
oscilloscope, GBIP
connector
Avocado fruits Ultrasonic Method Krautkramer USL 33 Wave velocity, attenua- Pre cooling and sizing Data normalization, non- R2 = 0.9946 [91]
tion coefficient, firm- of sample, ultrasonic linear regression
ness, dry weight, oil and penetration test,
content DW and oil content
determination

13

Table 4  (continued)
Fruit Cultivar Sensing technique details Instrumentation details Parameters Preprocess Data processing Model Accuracy Reference

13
Apple Pressure and Ultrasonic Ultrasonic pulse receiver Sugar content, firmness Scaling, Calibration, FE modeling – [92]
method (UPR) finite element, analysis
Tomato Ultrasonic method Low frequency system, Color, firmness, attenua- Stiffness factor calcula- Statistical modelling R2 = 0.95 [93]
USD10NF tion, time of flight tion, mean observation
Avocado and Mango Ultrasonic High-power, low- Firmness, attenuation, Correlation model Nonlinear regression R2 = 0.95 [94]
frequency ultrasonic velocity analysis
pulser-receiver (Kraut-
kramer Model USL33)
Apple Ultrasound Spectrometer Module Absorbance, stiffness, Non-destructive SSC prediction model R2 = 0.93 [95]
(MMS1 NIR-enhanced), firmness R2 = 0.91
Photo diode array detec-
tor (S4874-250Q)
Orange Ultrasound Harmonic wave func- Ultrasound velocity, Statistical analysis Correlation R2 = 0.874 [96]
tion generator (Sony dehydration R2 = 0.961
AFG320), power ampli-
fier (ENI 240L)
Mango Ultrasound LT374M Lecroy oscil- Ultrasound velocity, PCA, correlation Linear SSC and PLS R2 = 0.98 [97]
loscope, MM4005 attenuation model R2 = 0.81
Microcontroller based
S. Srivastava, S. Sadistap
Table 5  Major contribution in the area of machine vision based measurement for fruit quality assessment and authentication
Fruit cultivar Sensing technique Instrumentation details Parameters Preprocess Data processing Model accuracy Reference
details

Citrus Multispectral computer Customized experi- Identification of differ- RGB, HSI color extrac- LDA Success rate: 65–94% [103]
vision mental setup (CCD ent defects tion
(coupled charge
device) camera (Sony
XC-003P), UV and
NIR light source)
Apples (Jonagold) Machine vision (normal Customized conveyer Identification of infected Background segmenta- K-Mean clustering, Success rate: 73% [104]
image processing) belt based grading sampels tion, Color, shape and QDA
machine (Four lighting texture Extraction
tubes (690, 600 nm)
(18 W, type 33 from
Philips, The Nether-
lands), two cameras
(colour 3CCD CX003-
P from Sony, Japan)
Strawberry Hyperspectral imaging A laboratory hyperspec- Moisture, TSS, pH Corrected with a white MLR and PLS Moisture: 0.90, TSS: [105]
tral imaging system and a dark reference 0.80, pH: 0.87
was constructed (an
illumination unit
which consists of two
50 W halogen lamps
and CCD camera
(PCO-1600, Pco.
Non-destructive sensing methods for quality assessment of on-tree fruits: a review

Imaging, Germany))
Watermelon Machine vision (visible Image processing Volume Evaluation Grayscale, binary image, Ellipsoid approxima- Success rate: 95% [106]
image processing) system consisted of a outline detection tion, Bland–Altman
CMOS camera with approach
USB connection, a
fluorescent ring light
source (32 W), laptop
computer equipped
with ImageJ 1.36b,
Labview 6.1
Citrus Multispectral machine Customized experi- Color, size and shape Segmentation, edge Size and shape estima- Success rate: 93–94% [100]
vision mental setup along finding, color extrac- tion, surface inspec-
with conveyor belt tion, singularization tion, defect detection
with CCD camera
and illumination light
(classification between
mandarin and lemon
based on parameters)

13

Table 5  (continued)
Fruit cultivar Sensing technique Instrumentation details Parameters Preprocess Data processing Model accuracy Reference
details

13
Apple Multispectral machine Multispectral imaging Defect detection Corrected using dark 10-fold cross validation Success rate: 86–95% [107]
vision system was assembled images and reference technique with neural
(black and white CCD images (reflectance network
camera, UV-A fluores- (R), visible light
cent lamp, a tungsten induced fluorescence
halogen light source, (FVIS), and UV
seven band pass filters) induced fluorescence
(FUV))
Apple NIR imaging Charge-coupled device Defect Identification Color extraction, image PCA, ANN (Fletcher– Success rate: 77–93% [108]
(CCD) camera (Sony enhancement Reeves update
DFW-X700, Sony conjugate gradient
Corporation, Tokyo, algorithm)
Japan) mounted over
the conveyor
oranges, peaches, apples Machine vision (normal Machine vision system Length of damage, dam- Segmentation, color Fruit size, centroid loca- Success rate: 86–93% [109]
image processing) was composed of a aged area, overall fruit extraction (RGB, HIS, tion using morpho-
three charge cou- size and shape Lab), Bayesian discri- logical features
pled device (CCD) minant analysis
colour camera (Sony
XC003P) and a frame
grabber (Matrox
Meteor), connected to
a compatible personal
computer [Pentium
200 MHz, 48 Mb ran-
dom access memory
(RAM)]
Citrus Machine vision (visible CCD (charge-coupled Peel defect detection in Smoothing (peer group GLA (Generalized Success rate: 95% [110]
image processing) device) camera (Sony citrus filtering), histogram Lloyd Algorithm),
XC-003P), placed correction JSEG, Region-
400 mm above the growing iteration and
scene, provided the merging
video in the RGB (red,
green, blue) coordi-
nates
S. Srivastava, S. Sadistap
Table 5  (continued)
Fruit cultivar Sensing technique Instrumentation details Parameters Preprocess Data processing Model accuracy Reference
details

Apple Hyperspectral imaging Hyperspectral imaging Chilling Injury Detec- Global thresholding Color measurement, Success rate: 93% [111]
(400–1000 nm) system charge-coupled tion, firmness used for background BPANN
device (CCD) camera extraction
(PCO-1600, PCO
Imaging, Germany),
spectrograph (ImSpec-
tor V10E, Optikon
Co., Canada) coupled
with a standard
C-mount zoom lens
pomegranate Machine vision (visible Customized conveyer Detection of unwanted Pixel-oriented segmen- R/G ratio, Bayesian Success rate: 90% [112]
image processing) belt based grading part of pomegranate tation algorithm, color linear discriminant
machine system has fruit extraction analysis (LDA)
been developed
Mango (B74) Machine vision (visible Experimental setup con- Ripeness stage Lab and RGB color Statistical modelling Success rate: 55–69% [113]
image processing) sist (Two fluorescence extraction
lamps (TL-D Delux,
18W/965, Philips),
colour digital camera
(Olympus SP-500
UZ))
Strawberry Machine vision (visible Image processing part Shape, size and color Color extraction K-Mean clustering Classification: 90% [114]
Non-destructive sensing methods for quality assessment of on-tree fruits: a review

image processing) consists of camera


(WV-CP470, Pana-
sonic), image collect-
ing card (DH-CG300,
Daheng company)
Peach Multispectral imaging Frame grabber, National Firmness, SCC ANOVA Ward method Success rate: 82–87% [115]
Instruments, and a
three CCD custom
camera, MS-3100 with
three bandpass filters
centered at 800 nm
Infrared (IR), 675 nm
Red (R) and 450 nm
Blue (B), with a band-
width of 20 nm
Citrus Machine vision (visible 3-CCD camera (Sony Skin defect detection Color extraction, image PCA, T2 histogram and [116]
image processing) XC-003P), eight fluo- enhancement threshold analysis,
rescent tubes (daylight MIA method
type, 25W)

13
S. Srivastava, S. Sadistap

of food products and the prediction of their properties. fusion approaches used for food and beverage quality
Data fusion approaches could be classified in three dif- assessment and authentication. Authors explained funda-
ferent levels such as low, mid and high level data fusion mental of data fusion methods with their bottlenecks and
(Fig. 15). Other than data fusion, single and multilevel challenges. Few of the major efforts taken in the direction
prediction modelling have been explored in past research of multiple sensing technique fusion are summarized as
contributions. Borras et al. recently reviewed various data following (Table 6) [117].

Fig.  15  a Functional block diagram of different data fusion approach, b data processing methods used for fruit assessment and authentication
over the time period

13
Table 6  Major contribution in the area of non-destructive sensing techniques fusion for fruit quality assessment and authentication
Fruit cultivar Sensing technique Instrumentation Parameters Data fusion treat- Data processing Model accuracy Error Reference
fusion details ment

Apple Vis/NIR Spectros- A customized PC Firmness and SSC Simple concatena- PLS, PLS-DA Firmness: 0.93 Firmness: 7.73 [95]
copy and Acoustic based acoustic (on tree and shelf tion SCC: 0.20 SCC: 1.29
Sensing sensing system life)
and commercial
spectrometer
(400–1100 nm)
Grapes UV/NIR spectros- LCA 1000 E-Nose Fermentation time PCA, Genetic Algo- PLSR, PLS-DA Success rate: 90.4% Error: 9.6% [118]
copy and E-Nose and UV and FTIR rithm
Spectrometer
(Commercial)
Peaches Mechanical (impact Magness–Taylor Firmness, SSC and Simple concatena- PCA, MLR Success rate: 78.0% Error: 22.0% [119]
response) and opti- penetration force acidity (maturity, tion
cal sensing using impactor, harvest assess-
portable spec- ment)
trophotometer
Minolta CM-50I
(450–700 nm)
Wine Electronic Nose and PC based custom- Wine Quality and Identity declaration Fuzzy C-means, Success rate: Error: 5.6–16.7% [120]
electronic Tongue ized experimental Brand base clas- concatenation POFNN 83.3–94.4%
setup for E-tongue, sification
E-Nose
Apple Electronic Nose and Cyranose 320 (Con- Apple defect detec- Covariance matrix PCA, PNN, Bayes- Success rate: 89% Error: 11.0% [121]
Non-destructive sensing methods for quality assessment of on-tree fruits: a review

Acoustic Sensing ductive polymer tion adaptation evolu- ian network


Sensor) and Z-nose tionary strategy
(Surface acoustic (CMAES)
sensor)
Peaches Electronic nose and Commercial Firmness, SSC, Outer product PLS, PLS-DA Success rate: 93% Error: 7.0% [122]
visible spectros- Spectrometer Chlorophyll and analysis Feature
copy (300–1100 nm) acidity, Carotenoid extraction e PCA
and Libra Nose scores
Apple Electronic Nose, Commercial FTIR SSC, Sugar content (a) Variable selec- (a) NNs, MCR, PLS Success rate: 94% Error: 6.0% [123]
Machine Vision spectrometer, CCD and classification tion stepwise and PCR (b) Deci-
and NIR Spectros- color camera, cus- based on quality (b) Decision tree sion tree
copy tomized gas sensor
setup
Tomato Acoustic, weight Minolta CR 300 Ripening Stage Identity declaration Bayesian classifier Success rate: 95% Error: 5.0% [124]
and Colorimetric colorimeter, weigh- concatenation
Sensing ing instrument cus-
tomized acoustic
experimental setup

13

Table 6  (continued)
Fruit cultivar Sensing technique Instrumentation Parameters Data fusion treat- Data processing Model accuracy Error Reference
fusion details ment

13
Tomato Impact acoustic Minolta CR 300 Ripening Stage Identity declaration Bayesian classifier Success rate: 89% Error: 11.0% [125]
sensing and colori- colorimeter, Cus- concatenation (Bhattacharyya
metric sensing tomized Acoustic distance)
Experimental setup
Soybean flour NIR and MIR spec- MB160 FT-NIR Quality par. correla- Preprocessed data PLS Success rate: 95% Error: 5.0% [126]
troscopy spectrom- tion (crude protein
eter (ABB), FTLA and moisture)
2000 FTIR spec-
trometer
Wine Electronic nose and Electronic nose Classify four types Simple concatena- PCA, LDA and Success rate: 98.1% Error: 1.9% [127]
tongue (PEN2), custom- of Barbera wines tion CART
ized E-tongue
Experimental setup
Cherry tomato Electronic nose and PEN2 e-nose (Air SSC, Acidity Variable selection e PCA and CVA LVQ, SSC: 0.9970 Acid- SSC: 0.0056 Acid- [128]
juices tongue sense analytics), stepwise, factor F Lib-SVM and PCR ity: 0.9985 ity: 0.0553
alpha-Astree
e-tongue (Alpha
MOS company)
Apple Acoustic sensor, Vis- Vis-SWNIR spec- SSC, Firmness Variable selection e PLS SSC: 94.2% Firm- SSC: 5.8% Firm- [129]
SWNIR, HSI and trometer (S4000, specific for ness: 92.7% ness: 7.3%
bioyield tester Ocean Optics, each technique
Dunedin, FL),
portable firmness
tester, online hyper
spectral scattering
system (OHSS)
White grapes E-Nose, UV and LCA 1000, FTIR White grapes clas- Identity declaration PLS-DA, Bayesian Success rate: Error: 4.7% − 5.6% [130]
FTIR spectroscopy spectrometer sification concatenation inference 94.4–95.3%
(Bruker)
S. Srivastava, S. Sadistap
Non-destructive sensing methods for quality assessment of on-tree fruits: a review

Globally available solutions Discussion and conclusion

Various research efforts have been reported in the area of Different on-field issues during fruit harvesting time have
food quality assessment and authentication but very few of been raised and reported solutions in literature have been
the efforts have been translated in to the handheld proto- reviewed. Various non-destructive sensing methods used
type, which could be used to solve various on-field issues over the time period for fruit quality assessment and authen-
as mentioned above. Most of the reported research efforts tication have been discussed and compared with other sens-
are time consuming, bulky, need expert manpower for ing methods based on their advantaged and disadvantages.
operation and best suited for lab level applications. NIR Fundamental concepts of different non-destructive sens-
spectroscopy is one the common and mostly used method ing methods such as e-nose, UV–Vis–NIR spectroscopy,
in commercial available handheld system to predict on-tree machine vision and ultrasonic based sensing contributions
fruits quality parameters such as firmness, sugar content, have been explained in detail along with contribution of
ripeness stage and acidity. Most of the developed handy various researchers. Other non-destructive sensing meth-
spectroscopy systems are costly due to usage of expensive ods NMR, computer tomography etc are not considered for
NIR source, detectors, filters and other interface circuitry this review because those are time consuming, bulky and
but results consists higher repeatability and reliability. not suitable for field applications. Different non-destructive
Chemometric is majorly used for data processing and sensing techniques fusion approaches used for fruit quality
prediction model development in almost all spectroscopy assessment and authentication have been reviewed along
instruments. Other than spectroscopy, ultrasound based with their positive and negative points. Various portable
stiffness and firmness measurement is one of the widely instruments available for on-tree fruits quality inspection
used and cost effective method to predict on-tree fruit have been compared and reviewed based on their features
quality parameters as mentioned above. Measured firm- such as sensing technique, prediction variables, portability
ness and stiffness of the on-tree fruits further converted and cost. So many researchers have contributed in the area
in the form of various other quality parameters using sim- of non-destructive sensing methods for on-tree fruit quality
ple linear or non-linear statistical modelling. In most of inspection still very few global market products are avail-
the reported ultrasound sensing methods for fruit quality able to address few of the on-field issues. Even, most of the
assessment, contact type mode has been used for hard- systems available to address on field issues are very costly
ness measurement because non-contact ultrasound method and address very limited issues.
for fruit quality assessment have various limitations and
challenges such as distance-weight modelling, depth of
penetration, frequency range and size of transducers and Implications and future work
other interface electronics. Electronic nose using metal
oxide based gas sensors is also widely reported for on-tree Various fruit growers are facing different issues at different
fruit quality parameters. Limitations and bottlenecks with stages of the supply chain such as harvesting from field,
metal oxide based e-nose are cross selectivity and sensitiv- storage and precooling, drying, disinfection, grading, pack-
ity of the sensors due dynamic environmental conditions aging and transport. As all later stages are highly dependent
and baseline variation. Most of the solutions available on first stage related to quality, cost and losses. There is
in global market for on-tree fruit quality inspection are an essential requirement to solve on-field issues for mini-
based on contact type methods and uses single sensing mizing the losses as well as to harvest high quality fruits.
technique followed by single level prediction modelling. Even different vendors and fruit growers uses various artifi-
Most of the existing solution addresses harvesting time cial ripening methods using different chemicals and gases,
judgement issue while as other important issues such as which leads towards serious social implications related to
shelf life prediction and early warning for disease attack human health. Such kind of practices could be minimized
still need a cost effective, accurate and fast solution. There using the various electronic intervention to judge optimal
is no single effort has been identified in global market harvesting time for different fruits. Several efforts have been
related to multiple non-contact sensing techniques fusion identified in the direction of fruit quality assessment and
followed by predictive algorithmic intelligence to solve authentication but most of them are bulky, time consum-
various on-field issues such as early warning for disease ing, lab basis and requires skilled manpower to operate. Few
attack and related remedial action, harvesting time judge- of the efforts have been also recognized in the direction to
ment and many more. Fe of the efforts (Table 7) have been develop a handy instrument for fruit quality assessment and
identified in the direction to solve the problem of harvest- authentication but most of them are either destructive type
ing time judgement but most of them are very costly as or predicts very limited parameters. Future of fruit quality
well as have very limited features. assessment and authentication should be based on multiple

13

13
Table 7  Globally available handheld non-destructive sensing based fruit inspection systems
Instrument name and company Sensing technique details Instrumentation details Parameters Fruit cultivars Cost (INR) Reference
(approxi-
mate)

F-750 Handheld Brix and Dry Near-infrared (NIR) spectros- Handy instrument with graph- Total Soluble Solids (TSS or Cherry, apple, chico 5 Lakhs [131]
Matter Meter (Felix Ins., copy ics lcd and keys for human brix), titratable acidity, and
USA) interface, offers off tree color
testing
Nondestructive Fruit Tester Near-infrared (NIR) spectros- Handy Instrument with Graph- Total Soluble Solids (TSS or Citrus, apple, peer 20 Lakhs [132]
(H-100C) (Sunforest, Korea) copy ics LCD and Keys for Human brix), titratable acidity, and
Interface, offers on tree color
testing
DA-Meter (Turoni, Italy) Vis–Near-infrared (NIR) spec- Handy Instrument with graph- Measures amount of chloro- Apple 8 Lakhs [133]
troscopy ics LCD and keys for human phyll in fruit samples
interface, offers on tree testing
QS300, Portable instrument Vis-Near-infrared (NIR) spec- Handy Instrument with graph- Sugar content expressed in Brix Peaches, nectarines, apples, 5 Lakhs [134]
for fruit assessment (Unitech, troscopy ics LCD and keys for human degrees, acidity pears, kiwis
Italy) interface, offers off tree
testing
HFH80 Series Fruit and Ultrasonic Sensing Handy Instrument with Seven Measures stiffness and firmness Peaches, cherry, citrus 3.2 Lakhs [135]
Vegetable Ripeness Tester segment LCD and keys for
(Omega, USA) human interface, offers off
tree testing
Food Sniffer, Portable E-Nose Electronic Nose Pocket Size Module connectiv- Measures VOC, Temperature, Meat 10K [136]
(PRESS, USA) ity with smart phone Humidity, Ammonia Meas-
ures freshness
6003-E Fruit Firmness Tester Pressure based firmness tester Bench type system, best suited Firmness, stiffness Peaches, nectarines, apples, 25K [137]
(Vaiseshika Instruments, for lab applications pears, kiwis
India)
6007-A Refractometer Visible Spectroscopy (destruc- Handy module with keys and Sugar content Peaches, cherry, citrus 5K [138]
tive type) LCD for human interface
S. Srivastava, S. Sadistap
Non-destructive sensing methods for quality assessment of on-tree fruits: a review

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