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  • Basilus Subtilis: Banana Leaves Extract

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    Ruby Cocal
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    This document reports the results of an experiment testing the antibacterial properties of banana leaf, peel, and stem extracts against Bacillus subtilis and Escherichia coli bacteria. The extracts were tested at different concentrations (5, 15, 30, 60 mg/ml) and time periods (24, 48, 72 hours) by measuring zone of inhibition. The ethanolic extracts generally showed higher inhibition zones than the aqueous extracts. Zone of inhibition increased with higher extract concentrations and longer exposure times, indicating the banana extracts have antibacterial effects in a dose-dependent manner.

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    This document reports the results of an experiment testing the antibacterial properties of banana leaf, peel, and stem extracts against Bacillus subtilis and Escherichia coli bacteria. The extracts were tested at different concentrations (5, 15, 30, 60 mg/ml) and time periods (24, 48, 72 hours) by measuring zone of inhibition. The ethanolic extracts generally showed higher inhibition zones than the aqueous extracts. Zone of inhibition increased with higher extract concentrations and longer exposure times, indicating the banana extracts have antibacterial effects in a dose-dependent manner.

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    2 views4 pages

    Basilus Subtilis: Banana Leaves Extract

    Uploaded by

    Ruby Cocal
    This document reports the results of an experiment testing the antibacterial properties of banana leaf, peel, and stem extracts against Bacillus subtilis and Escherichia coli bacteria. The extracts were tested at different concentrations (5, 15, 30, 60 mg/ml) and time periods (24, 48, 72 hours) by measuring zone of inhibition. The ethanolic extracts generally showed higher inhibition zones than the aqueous extracts. Zone of inhibition increased with higher extract concentrations and longer exposure times, indicating the banana extracts have antibacterial effects in a dose-dependent manner.

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    of 4

    Test Banana Leaves Extract

    Organisms Treatments Replicates ZONE OF INHIBITION (mm)


    Bacteria Aqueous Extract Ethanolic Extract
    24 hr. 48 hr. 72 hr. 24 hr. 48 hr. 72 hr.
    Basilus T0 (negative R1 0 0 0 0 0 0
    subtilis control) R2 0 0 0 0 0 0
    R3 0 0 0 0 0 0
    T1 (5mg/3ml) R1 0 0 0 0 0 0
    R2 0 0 0 3 5 7
    R3 0 0 0 5 6 7
    T2 R1 0 0 0 5 7 8
    (15mg/3ml) R2 0 0 0 7 8 10
    R3 0 0 0 10 11 13
    T3 R1 0 0 0 9 10 11
    (30mg/3ml) R2 0 0 0 8 9 12
    R3 0 0 0 17 18 19
    T4 (60mg/ml) R1 0 0 0 10 13 14
    R2 0 0 0 12 13 15
    R3 0 0 0 14 15 17
    T5 (positive R1 14 15 16 13 14 15
    control) R2 12 13 15 14 16 17
    R3 16 17 17 16 17 17
    Eschirechia T0 (negative R1 0 0 0 0 0 0
    coli control) R2 0 0 0 0 0 0
    R3 0 0 0 0 0 0
    T1 (5mg/3ml) R1 0 0 0 0 0 0
    R2 0 0 0 3 4 4
    R3 0 0 0 0 0 0
    T2 R1 0 0 0 3 6 6
    (15mg/3ml) R2 0 0 0 4 6 7
    R3 0 0 0 5 6 6
    T3 R1 0 0 0 4 4 4
    (30mg/3ml) R2 0 0 0 4 4 4
    R3 0 0 0 5 6 6
    T4 (60mg/ml) R1 0 0 0 7 6 7
    R2 0 0 0 8 11 11
    R3 0 0 0 6 7 7
    T5 (positive R1 13 14 16 13 14 14
    control) R2 17 18 18 12 14 14
    R3 14 15 17 14 14 14
    Test Banana Peel Extract
    Organisms Treatments Replicates ZONE OF INHIBITION (mm)
    Bacteria Aqueous Extract Ethanolic Extract
    24 hr. 48 hr. 72 hr. 24 hr. 48 hr. 72 hr.
    Basilus T0 R1 0 0 0 0 0 0
    subtilis (negative R2 0 0 0 0 0 0
    control) R3 0 0 0 0 0 0
    T1 R1 0 0 0 3 3 4
    (5mg/3ml) R2 0 0 0 5 5 5
    R3 0 0 0 5 5 7
    T2 R1 0 0 0 6 6 7
    (15mg/3ml) R2 0 0 0 8 8 9
    R3 0 0 0 9 9 9
    T3 R1 0 0 0 11 11 12
    (30mg/3ml) R2 0 0 0 10 12 12
    R3 0 0 0 10 11 11
    T4 R1 0 0 0 13 13 14
    (60mg/ml) R2 0 0 0 13 13 13
    R3 0 0 0 12 12 13
    T5 (positive R1 14 16 17 15 15 15
    control) R2 15 16 16 14 14 16
    R3 12 13 14 17 18 18
    Eschirechia T0 R1 0 0 0 0 0 0
    coli (negative R2 0 0 0 0 0 0
    control) R3 0 0 0 0 0 0
    T1 R1 0 0 0 0 3 4
    (5mg/3ml) R2 0 0 0 0 4 4
    R3 0 0 0 0 3 3
    T2 R1 0 0 0 0 3 5
    (15mg/3ml) R2 0 0 0 3 4 6
    R3 0 0 0 0 3 4
    T3 R1 0 0 0 6 6 6
    (30mg/3ml) R2 0 0 0 7 8 10
    R3 0 0 0 6 6 7
    T4 R1 0 0 0 9 11 11
    (60mg/ml) R2 0 0 0 10 12 13
    R3 0 0 0 7 7 8
    T5 (positive R1 15 16 16 13 14 14
    control) R2 13 14 16 14 14 15
    R3 13 15 16 11 14 15
    Test Banana Stem Extract
    Organisms Treatments Replicates ZONE OF INHIBITION (mm)
    Bacteria Aqueous Extract Ethanolic Extract
    24 hr. 48 hr. 72 hr. 24 hr. 48 hr. 72 hr.
    Basilus T0 R1 0 0 0 0 0 0
    subtilis (negative R2 0 0 0 0 0 0
    control) R3 0 0 0 0 0 0
    T1 R1 0 0 0 4 5 6
    (5mg/3ml) R2 0 0 0 3 4 5
    R3 0 0 0 3 5 6
    T2 R1 0 0 0 5 7 7
    (15mg/3ml) R2 0 0 0 3 4 6
    R3 0 0 0 6 7 9
    T3 R1 0 0 0 11 13 14
    (30mg/3ml) R2 0 0 0 8 11 13
    R3 0 0 0 9 11 11
    T4 R1 0 0 0 12 14 15
    (60mg/ml) R2 0 0 0 10 13 15
    R3 0 0 0 9 12 13
    T5 (positive R1 12 13 15 13 13 14
    control) R2 14 16 17 14 16 17
    R3 14 15 16 13 14 16
    Eschirechia T0 R1 0 0 0 0 0 0
    coli (negative R2 0 0 0 0 0 0
    control) R3 0 0 0 0 0 0
    T1 R1 0 0 0 4 6 6
    (5mg/3ml) R2 0 0 0 3 5 5
    R3 0 0 0 3 3 4
    T2 R1 0 0 0 6 6 7
    (15mg/3ml) R2 0 0 0 5 5 5
    R3 0 0 0 5 6 6
    T3 R1 0 0 0 6 7 7
    (30mg/3ml) R2 0 0 0 6 6 7
    R3 0 0 0 6 6 6
    T4 R1 0 0 0 8 8 9
    (60mg/ml) R2 0 0 0 6 7 7
    R3 0 0 0 6 8 10
    T5 (positive R1 13 14 15 11 14 14
    control) R2 15 16 17 8 10 11
    R3 12 14 16 7 9 11

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