Lab #3 –Cellular Respiration Simulation /20

Learning Outcomes:
 Understanding basic concepts about metabolism and energy production in the
cell.
 Learning the main catabolic pathways that make up the -cellular respiration:
Glycolysis, Lactic acid fermentation, and Krebs cycle.
 Understanding the electron transport chain and the role of redox electron
carriers.
 Understanding the concepts of oxidative phosphorylation, chemiosmosis, and
electrochemical gradients.
 Calculating the amount of ATP produced in the different metabolic routes.
 Achieving an integrated view of the whole cellular respiration process and energy
use via respirometry experiments.
 Understanding the importance and good use of animal models in
experimentation.

Part A – Cellular Respiration Review (7 marks total)

1. For each of the following statements, choose the appropriate option by
highlighting it in yellow. (3 marks)

a. Catabolic / Anabolic reactions break down big molecules to obtain energy.

b. The primary energy source for mammals is carbohydrates / fats.
c. The 3-carbon molecule that results from glycolysis is acetyl-CoA / pyruvate.
d. Anaerobic / Aerobic respiration does not need oxygen to occur.
e. The final electron acceptor in the electron transport chain is water / oxygen.
f. ATP / ADP is considered the energy currency of the cell.

2. Complete the following table which summarizes the details of cellular respiration.
(4 marks)

Where in the cell it takes Product(s) # of ATP

Stage [including waste products;
place amounts not needed] produced
Glycolysis Click here Click here Click here
NADH, FADH2, carbon Click here
Krebs Cycle Click here
dioxide
Electron
Click here Click here Click here
Transport Chain
Part B – Respirometry Experiment (9 marks total)
3. For each of the following statements, choose whether it is true or false by
highlighting the appropriate answer in yellow. (3 marks)

a. True / False: An advantage of using animal models for experiments in the lab
is the affordability.
b. True / False: Mice and rats are the most commonly used lab animals because
they are similar to humans and easy to maintain.
c. True / False: Respirometry measures oxygen consumption.
d. True / False: The two parameters in the respirometer that can be modified are
glucose usage and exercise intensity.
e. True / False: When the mouse starts running on the treadmill, oxygen
consumption will presumably decrease.
f. True / False: When the mouse first starts exercising, the main source of ATP is
lactic acid fermentation.

4. Fill in the blanks to complete the following statements. (4 marks)

a. In order to test what happens under extreme exercise conditions, the test was
done at high __________ and low ___________ availability.

b. The pain we feel after exercising at a very high intensity is a direct result of the
massive amount of _________________ produced.

c. The best way to test the importance of a certain metabolic route in an

organism is to __________ it and measure the effect.

5. What chemical was measured in the simulation to assess the importance of

aerobic respiration for energy production in our cells? Choose only one answer.
(1 mark)
☐ Lactic acid
☐ Carbonyl cyanide m-chlorophenylhydrazone (CCCP)
☐ Potassium cyanide (KCN)
☐ ATP synthase
 6. Which of the following is an example of an electron carrier?
Choose only one answer. (1 mark)
☐ FAD
☐ NADH
☐ CO2
☐ O2

Part C – Final Conclusions (4 marks)

7. What were the final conclusions of the lab experiment? Select all that apply. (2
marks)

☐ Physical activity increases oxygen requirements

☐ Oxygen is not essential for a sufficient energy production
☐ Oxygen is essential for a sufficient energy production
☐ Lactic acid fermentation can also provide enough energy for exercising
☐ Athletic training should avoid extreme conditions that could lead to lactic
acidosis
☐ If not enough oxygen is available, lactic acid is accumulated

8. Why would training at high altitudes benefit the football players? (2 marks)

Click here to insert your answer.