TissueLyser II

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For medium- to high-throughput sample disruption for molecular

analysis
 Convenient and secure disruption process
 Adapter sets optimized for high-throughput disruption
 Wide range of accessories available
 Reproducible results with difficult-to-lyse tissues
 Front-end solution for QIAGEN automation

The TissueLyser II simultaneously disrupts multiple biological samples through high-speed shaking
in plastic tubes with stainless steel, tungsten carbide, or glass beads. Using the appropriate adapter
set, up to 48 or 192 samples can be processed at the same time. Alternatively, a grinding jar set can
be used to process large samples. A range of beads, bead dispensers, and collection microtubes
and caps are also available. All accessories for the TissueLyser II, including adapter sets, are also
compatible with the TissueLyser (no longer available).

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Cat No./ID: 85300

TissueLyser II

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Bead mill, 100–120/220–240 V, 50/60 Hz; requires the TissueLyser Adapter Set 2 x 24 or TissueLyser Adapter Set 2 x 96 (ava
 The TissueLyser II is intended for molecular biology applications. This product is not intended for the
diagnosis, prevention, or treatment of a disease.

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Reproducible RNA purification from plant tissues.
Frozen plant leaves were disrupted using the TissueLyser II (2 x 1 minute). RNA was purified using
the RNeasy Plant Mini Kit and analyzed on a 1.2 % formaldehyde agarose gel. The ribosomal RNA
bands were sharp and of equal intensity, indicating reproducible purification of intact RNA. T: tomato
(100 mg); A: arabidopsis (25 mg); C: cotton (100 mg); M: maize (100 mg); R: rape (100 mg).

Performance
The TissueLyser II is well-suited for high-throughput disruption of human, animal, and plant tissues,
bacteria, and yeast. Highly reproducible purification of high-quality DNA, RNA, miRNA, and protein is
achieved, even with difficult-to-lyse tissues (see figures "Efficient disruption of animal tissues",
"Reproducible RNA purification from plant tissues", "Reproducible DNA purification from
animal tissue", "Purification of intact protein from animal tissue", "Reproducible RNA
purification from Gram-positive bacteria", and "Reliable detection of miRNAs").

Principle

Genotyping, gene expression, and proteomics applications demand effective disruption of biological
samples to ensure high yields of DNA, RNA, and protein. Effortless disruption with the TissueLyser II
system is achieved through high-speed shaking with beads, which beat and grind samples. The
TissueLyser II system delivers thorough and rapid disruption of samples to fully release
biomolecules, and also simultaneously homogenizes samples to facilitate subsequent purification
procedures using QIAGEN kits (see table "QIAGEN purification kits compatible with QIAGEN
disruption systems").

The TissueLyser II is an integral part of QIAGEN's complete solution for sample management —
from sample collection to purification and analysis of DNA, RNA, and protein. The TissueLyser II
complements QIAGEN automation for high-throughput sample preparation and analysis (see table
"QIAGEN high-throughput automation"), such as the QIAsymphony SP. This easy-to-use instrument
automates purification of DNA, RNA, and protein from 1–96 samples. The TissueLyser II is also
compatible with QIAGEN manual sample preparation kits (see table "QIAGEN purification kits
compatible with QIAGEN disruption systems").

For RNA applications, stabilization of fresh tissues in RNAlater RNA Stabilization Reagent prevents
RNA degradation during sample handling. For applications requiring purification of DNA, RNA, and
protein, these 3 analytes can be immediately stabilized by placing fresh tissues in Allprotect Tissue
Reagent.

QIAGEN high-throughput automation

Instrument Purpose

QIAsymphony SP Purification of DNA, RNA, and protein

BioRobot Universal System Purification of DNA and RNA

QIAxcel Electophoretic analysis of DNA fragments and RNA

QIAgility Reaction setup

Rotor-Gene Q Real-time PCR and high-resolution melting (HRM) analyses

PyroMark Q96 MD or ID Methylation and mutation analyses

QIAGEN purification kits compatible with QIAGEN disruption systems

Analyte purified Sample type QIAGEN kit

 RNeasy Kits

RNA Easy-to-lyse tissue

RNeasy Plus Kits

RNeasy Protect Kits

RNA Fiber-rich tissue RNeasy Fibrous Tissue Kits

RNeasy Lipid Tissue Kits

RNA All types of tissue
RNeasy Universal Tissue Kits

RNA Plant tissue RNeasy Plant Mini Kit

RNA Yeast RNeasy Kits

RNA Bacteria RNeasy Protect Bacteria Kits

microRNA All types of tissue miRNeasy Kits

DNA Human tissue QIAamp DNA Kits

DNA Animal tissue DNeasy Blood & Tissue Kits

Protein Tissue Qproteome Mammalian Protein

Phosphoprotein Tissue PhosphoProtein Purification Kit

Glycoprotein Tissue Qproteome Glycoprotein Kits

 DNA and RNA Tissue AllPrep DNA/RNA Kits

DNA, RNA, and protein Tissue AllPrep DNA/RNA/Protein Min

Procedure

TissueLyser Adapter Sets allow processing of up to 2 x 96 samples in collection microtubes or up to

2 x 24 samples in 2 ml microcentrifuge tubes. The adapter sets can be precooled at –80°C if
disrupting samples without lysis buffer. Tubes remain securely sealed during disruption to prevent
cross-contamination, which is especially important for highly sensitive downstream applications,
such as real-time RT-PCR or microarray analysis. Depending on the sample type, disruption is
carried out using stainless steel, tungsten carbide, or glass beads. TissueLyser Bead Dispensers are
available to conveniently deliver single beads into microcentrifuge tubes or to deliver 96 beads in
parallel into collection microtubes. The TissueLyser II can also disrupt large samples when used in
combination with Grinding Jar Sets.

Applications

The ability to process up to 192 samples per run makes the TissueLyser II the ideal front-end
solution to access biological information for genomics, transcriptomics, and proteomics applications.
For next-generation, high-throughput sequencing technologies, the TissueLyser II is the disruption
instrument of choice. A wide range of QIAGEN sample purification kits are compatible with the
TissueLyser II, and sample purification can be performed manually or can be automated using the
QIAcube, QIAsymphony SP, EZ1 Advanced, or BioRobot or BioSprint workstations.

Features Specifications

Disruption principle High-speed shaking of samples in 1.2 ml collection tubes or 2 ml microcentrifuge tubes with s

Convenient and secure disruption process. Adapter sets optimized for high-throughput disrupti
Features (e.g.,grinding jar set to process large samples). Reproducible results with difficult-to-lyse tissu
automation.

Kits compatible with

All kits for purifying RNA/DNA/Protein
instrument
Features Specifications

Protocol/main application
Sample preparation/sample disruption
on this intrument

Technical data 100–120/220–240 V, 50/60Hz; variable speeds from 3 to 30 Hz (180–1800 oscillations/minute

Technology Bead Mill

Throughput 2 x 96 collection microtubes (1.2 ml) or 2 x 24 microcentrifuge tubes (2ml)

Typical run time 15 sec - 2 x 3 minutes at 15–30 Hz

FAQs (1)
Kit Handbooks (1)
Supplementary Protocols (8)
User-Developed Protocols (11)
Instrument User Manuals (1)
Brochures & Guides (1)

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How to order

Online: www.qiagen.com/shop

By email: orders-bnl@qiagen.com

By fax: 8002 2073

By phone: 8002 2076

By mail: QIAGEN Benelux B.V.
Hulsterweg 82
5912 PL Venlo
The Netherlands

How to contact us

By email: customercare-bnl@qiagen.com

By fax: 8002 2073

By phone: 8002 2076

By mail: QIAGEN Benelux B.V.

Hulsterweg 82
5912 PL Venlo
The Netherlands

Office hours: Normal business hours are 09:00 – 17:00, Monday to Friday (excluding holidays)

Outside of our working hours, you can leave recorded messages for our immediate
attention the following work day