NAMA : FEBIOLA SILVIA NINGSIH

NIM : 171810301042
KELAS : KINETIKA A

SOAL

The enzyme carboxypeptidase catalyses the hydrolysis of polypeptides, and here we consider
its inhibition. The following results were obtained when the rate of the enzymolysis of
carbobenzoxy-glycyl-D-phenylalanine (CBGP) was monitored without inhibitor:
[CBGP] (M) 1.25×10-2 3.84×10-2 5.81×10-2 7.13×10-2
Uninhibited Relative Reaction Rate 0.398 0.669 0.859 1.00
(All rates in this exercise were measured with the same concentration of enzyme and are
relative to the rate measured when [CBGP] = 0.0713 M in the absence of inhibitor.) When
2.0 mM phenylbutyrate ion was added to a solution containing the enzyme and substrate, the
following results were obtained:
[CBGP] (M) 1.25×10-2 2.50×10-2 4.00×10-2 5.50×10-2
Inhibited Relative Reaction Rate 0.172 0.301 0.344 0.548
In a separate experiment, the effect of 50 mM benzoate ion was monitored and the results
were:
[CBGP] (M) 1.75×10-2 2.50×10-2 5.00×10-2 10.00×10-2
Inhibited Relative Reaction Rate 0.183 0.201 0.231 0.246

JAWAB :
1. Biasa

c v 1/c 1/v
1.25 0.398 0.8 2.512563
3.84 0.669 0.260417 1.494768
5.81 0.859 0.172117 1.164144
7.13 1 0.140252 1
Grafik :

Enzim dan Substrat

3

2.5 y = 2.1747x + 0.7965

R² = 0.9795
2

1.5

0.5

0
0 0.2 0.4 0.6 0.8 1

2. Inhibitor 1

c v 1/c 1/v
1.25 0.172 0.8 5.813953
2.5 0.301 0.4 3.322259
4 0.344 0.25 2.906977
5.5 0.548 0.181818 1.824818
Grafik :

Penambahan Fenilbutirat
7
6 y = 6.0086x + 1.0158
R² = 0.9718
5
4
3
2
1
0
0 0.2 0.4 0.6 0.8 1

3. Inhibitor 2

c v 1/c 1/v
1.75 0.183 0.571429 5.464481
2.5 0.201 0.4 4.975124
5 0.231 0.2 4.329004
10 0.246 0.1 4.065041
Grafik 2 :

Penambahan Ion Benzoat

6

2 y = 3.0099x + 3.7517
R² = 0.999
1

0
0 0.1 0.2 0.3 0.4 0.5 0.6 0.7

Grafik Gabungan :

gabungan
7 y = 6.0086x + 1.0158
y = 3.0099x + 3.7517 R² = 0.9718
6 R² = 0.999

2
y = 2.1747x + 0.7965
1 R² = 0.9795

0
0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9

Berdasarkan grafik yang diperoleh diatas yaitu grafik tersebut termasuk jenis inhibisi
campuran (mixed), dikarenakan tidak bertemu pada satu titik yang sama dan memiliki
intersep yang beda.