Untitled

SECTION 2 HYDRAULICS
2.1 PNEUMATIC SYSTEM........................................................................................................................1
2.2 USE OF PRESSURE AND VACUUM .................................................................................................2
2.3 HYDRAULIC SYSTEM ........................................................................................................................3
2.4 ANALYSIS PARAMETER ....................................................................................................................4
2.5 REAGENT SYSTEM ...........................................................................................................................6
2.6 ANALYSIS FLOW................................................................................................................................7
2.7 FCM DETECTOR BLOCK...................................................................................................................8

2.7.1 Function .............................................................................................................................9
2.8 OPTICAL SYSTEM ...........................................................................................................................11
2.9 HYDRAULIC SEQUENCE DESCRIPTION .......................................................................................12
2.10 HYDRAULIC PARTS .......................................................................................................................33

2.10.1 Solenoid Valve Function ................................................................................................33
2.10.2 HYDRAULIC PARTS LOCATION ..................................................................................35
2.11 SRV STRUCTURE ..........................................................................................................................40
2.12 CONVERSION TABLE OF VACUUM AND PRESSURE VALUES..................................................42
2.13 Hydraulic System Difference Between XE-2100 and XE-2100 DC ........................................44

2.13.1 XE-2100 Hydraulic System ............................................................................................44
2.13.2 XE-2100DC (XE-2100C) Hydraulic System...................................................................44
XE-series S/M February 5, 2009

SECTION 2 HYDRAULICS
2.1 PNEUMATIC SYSTEM
The system to provide the required pressure and vacuum for main unit operation consists of the
following. Pressurized air is regulated at 2.5 kg/cm2 by a relief valve in the pneumatic unit, any dust and
water droplets are removed by an air filter. An air drier is used to remove any remaining moisture before
it is supplied to the main unit. The pressure of the dry air is regulated at 1.6, 0.7, and 0.3 kg/cm2.
The vacuum (400 mmHg or higher) is adjusted to 300 mmHg by the bellows unit in the main unit.
A filter is provided on the bellows unit to prevent the needle in the bellow from accumulating dirt.
The outline of pneumatic control system is shown below.
XE-series S/M 2-1 February 5, 2009

2.2 USE OF PRESSURE AND VACUUM
Use of pressure and vacuum for the main unit are as follows.
Type Name Functions
2
Pressure 2.5 kg/cm Driving master valves, air cylinders, and diaphragm pump (DP 5mL for
FCM (EPK) replenishment)
1.6 kg/cm2 Sheath Flow forming for FCM
0.7 kg/cm2 Draining waste chamber and driving of diaphragm pumps
0.3 kg/cm2 Sheath Flow forming for RBC
Vacuum Source Vacuum Aspiration of reagent and driving diaphragm pumps.
300 mmHg Aspiration of waste to WC1

2.3 HYDRAULIC SYSTEM
WBC/RBC Block Diagram
RBC/HGB Block Diagram

2.4 ANALYSIS PARAMETER
Analysis Block RBC/PLT HGB IMI

Principle Searth Flow DC Detection Method Colorimetric RF/DC
Analysis RBC/PLT Channel HGB Channel IMI Channel
Channel
Information PLT Histogram RBC IMI Scattergram*5
Histogram
Parameter PLT HCT RBC HGB -
MCV,MCH,MCHC,
Analytical MPV,PDW, P- RDW-SD/CV
Parameter LCR
Reagent CELL PACKII / SE SEATHII SULFOLYSER STOMATOLYSER-IM
Analysis Block WBC/RET

Principle Flowcytometry by using Semi-Conductor Laser
Analysis WBC/BASO 4DIFF Channel NRBC RET
Channel Channel Channel Channel
Information WBC/BASO 4DIFF Scattergram *1 NRBC RET
Scattergram*2 Scattergra Scattergra
m*3 m*4
Parameter WBC# BASO Lymph MONO NEUT EOSI NRBC% RET%
% % % % % NRBC# RET# PLT-
BASO Lymph MONO NEUT EOSI O
# # # # #
Analytical L/M/HFR,
Parameter IRF
Reagent STROMATOLYS STROMATOLYSER-4D STROMAT RET
ER-FB Lysing solution/ Staining Dye (NEW) OLYSER- SERACHII
NR Lysing Diluent
solution/ solution/
Staining Staining
Dye (NEW) Dye (NEW)

*1 4DIFF Scattergram *2 WBC/BASO Scattergram
*3 NRBC Scattergram *4 RET Scattergram
*5 IMI Scattergram
Figure 2-1 Scattergrams

2.5 REAGENT SYSTEM
Figure 2-2 Reaction Chamber Construction

2.6 ANALYSIS FLOW

2.7 FCM DETECTOR BLOCK
NOTE:
Laser Diode is very sensitive to static electricity. Follow the instruction below
when you replace the Laser Diode.
- Remove jumper pin from the terminal on the Laser Diode AFTER connect-
ing to PCB No. 2140. If the jumper pin is removed before connecting to
PCB No.2140, Laser Diode will be damaged.
- Do not disconnect the connector to the PCB No.2140.
In the worst case, the device inside the Laser Diode will be destroyed.
The life of the Laser Diode is approx. 10,000 hours.

2.7.1 Function
(1) Outline
Laser diode apply to samples in sheath flow generated flow cell. FCM Detector Block detects front
scattered light, side scattered light, and side fluorescence light from these samples. XE-2100 gen-
erates two-dimensional scattergrams from these signals as follows.
X-axis Y-axis
WBC# / BASO SSCL FSCP
DIFF SSCL SFLP
NRBC SFLL FSCP
RET SFLP FSCP
PLT-O SFLP FSCL
SSCL: Side SCattered peak Logarithm

FSCL: Front SCattered peak Logarithm
SFLP: Side Fluorescence Light Peak linear
SFLL: Side Fluorescence Light peak Logarithm
FSCP: Front SCattered Peak linear
Following table shows which receiver is used for each parameter.

FSC Photo diode Side Scattered PMT Side Fluorescence PMT
WBC# / BASO Used Used Not Used
DIFF Used but not Used Used
displayed in
Scattergram
NRBC Used Not Used Used
RET Used Not Used Used
(2) Beam spot generating system

Beam spot generating system consists of laser diode, collimator lens. Laser beam generated by
laser diode is converted to parallel beam by collimator lens. This beam is collected by condenser
lens and applies to samples. Beam shape is as follows.
(3) Sheath flow system

Sheath flow is generated in the flow cell. Sample flows center of the flow cell.
(4) Front scattered light system
Front scattered light system consists of beam stopper and FSC condenser lens.
Beam stopper shade direct light from the laser diode. FSC condenser lens collect the scattered
light of the sample.
(5) Dichroic mirror
Light below 640 nm wave length reflects to the side scattered light PMT.

(6) Side fluorescence light system
Side fluorescence light system consists of interference filter, PMT (Photo Multiplier Tube). The light
through dichroic mirror is filtered by interference filter and fluorescence light over 660 nm wave
length is detected by PMT. Detected fluorescence light signal is amplified by pre-amplifier and
transmitted to main amplifier.
(7) Side Scattered light system
Side Scattered light system consists of ND filter (Neutral Density filter) and PMT.
The light through dichroic mirror is attenuated by ND filter and scattered light is detected by PMT.
Detected scattered light signal is amplified by pre-amplifier and transmitted to main amplifier.
(8) Temperature Control
FCM Detector block is heater controlled to maintain the optical stability.
(9) Cover switch
Cover switch is installed to prevent interference from the outer light and leakage of the laser beam.
If the cover is opened, XE-2100 stops laser radiation and massage is displayed.

2.8 OPTICAL SYSTEM
XE-2100 S/M 2-11 February 5, 2009

2.9 HYDRAULIC SEQUENCE DESCRIPTION
0- 5 sec
[WB aspiration, reagent chamber]
- SRV turns activating SV60 for 0 - 0.5 sec.
- Whole blood pump descend to aspirate diluent for 0 - 2.0 sec.
- SRV is rinsed (perimeter cleaning) by activating SV60 for 0 - 0.5.sec.
- WC2 is drained by activating SV 43 for 1.5 - 7.5 sec.
- SNR is replenished by activating SV8 for 0 - 3.0 sec.
[HGB]
- Solution in HGB flow cell is drained by activating SV36 for 3.0 - 3.8 sec.
- HGB Diluent DP dispense diluent into HGB flow cell for rinsing for 3.8 - 4.9 sec.
- SV 11 is activated to push the solution in the air mixing tubing to WC1.
XE-2100 S/M 2-12 February 5, 2009

XE-2100 S/M 2-13 February 5, 2009
5 - 10 sec
- Whole blood pump descend to aspirate sample in the second stage for 7.8 - 7.9sec.
- SV 54 is activated to switch the path to sample line for 9.3 - 17.7 sec.
- Diluent is dispensed to rinse cup by activating SV56 and drained into WC1 by activating SV59
while rinse cup descends for clean pipette outside.
- Sheath waste chamber is drained by activating SV 69 for 5.7 - 7.5 sec.
[BASO]
- Solution in BASO reaction chamber is drained by activating SV 40 for 8.7 9.4 sec.
- Lyse reagent is dispensed to BASO reaction chamber by activating SV19 for 9.4 - 10.6 sec for
rinsing.
- Mixing motor at reaction chamber rotates for 9.4 - 10.5 sec.
[DIFF]
- Solution in DIFF reaction chamber is drained by activating SV38 for 8.7 - 9.4 sec.
- Lyse reagent is dispensed to DIFF reaction chamber by activating SV34 for 9.4 - 10.6 sec for rins-
ing.
[NRBC]
- Solution in NRBC reaction chamber is drained by activating SV39 for 8.7 - 9.4 sec.
- Diluent is dispensed to NRBC reaction chamber by activating SV34 for 9.4 - 10.6 sec for rinsing.
[RET]
- Solution in RET reaction chamber is drained by activating SV37 for 8.5 - 9.1 sec.
- Diluent is dispensed into RET reaction chamber by activating SV32 for 9.6 - 10.6 sec for rinsing.
- Diluent is dispensed into RET reaction chamber by activating SV49 for 9.1 - 9.6 sec for rinsing.
[HGB]
- Solution in HGB flow cell is drained by activating SV36 for 4.9 - 5.8 sec.
- HGB Diluent DP dispense diluent into HGB flow cell for rinsing for 5.8 - 7.8 sec.
[RBC]
- Solution in RBC sample chamber is drained by activating SV65 for 6.7 - 8.2 sec.
- RBC Diluent DP dispense diluent into RBC sample chamber for 8.2 - 9.7 sec.
[IMI]
- Solution in IMI detector chamber is drained by activating SV14 for 7.5 - 9.1 sec.
- IMI lyse reagent DP dispense reagent into IMI detector chamber for 7.5 - 8.4 sec.
- IMI detector chamber is air mixed by activating SV12.
XE-2100 S/M 2-14 February 5, 2009

XE-2100 S/M 2-15 February 5, 2009
10 - 15 sec.
- Whole blood pump descend to aspirate diluent for 9.3 - 12.3 sec.
- Solution in WC1 is drained by activating SV57 for 11.8 - 14.6 sec.
- EPK is replenished by activating SV 10 for 12.6 - 16.4 sec.
[BASO]
- BASO reaction chamber is air mixed by activating SV13.
- Solution in BASO reaction chamber is drained by activating SV40 for 10.5 - 14.6 sec.
- Lyse reagent is dispensed with sample to BASO reaction chamber by activating SV19 for 11.6 -
14.1 sec.
- Mixing motor at reaction chamber rotates for 11.3 - 14.9 sec for sample mixing.
[DIFF]
- Solution in DIFF reaction chamber is drained by activating SV38 for 10.5 - 11.5 sec.
- Lyse reagent is dispensed with sample to DIFF reaction chamber by activating SV33 for 11.6 -
14.1 sec.
- Stain reagent is dispensed with sample to DIFF reaction chamber by activating SV63 for 12.5 -
14.1.
[NRBC]
- Solution in NRBC reaction chamber is drained by activating SV39 for 10.5 - 11.5 sec.
- Diluent is dispensed with sample to NRBC reaction chamber by activating SV34 for 11.6 - 14.1
sec.
- Stain reagent is dispensed with sample to NRBC reaction chamber by activating SV62 for 12.5 -
14.1.
[RET]
- Solution in RET reaction chamber is drained by activating SV37 for 10.6 - 11.7 sec.
- Diluent is dispensed with sample into RET reaction chamber by activating SV32 for 11.8 - 14.3
sec for rinsing.
- Stain reagent is dispensed with sample to RET reaction chamber by activating SV64 for 12.7 -
14.3.
[HGB]
- Solution in HGB flow cell is drained with sample by activating SV36 for 10.0 - 11.4 sec.
- HGB Diluent DP dispense diluent into HGB flow cell by activating SV15 for 11.4 - 14.0 sec.
- HGB lyse DP dispense diluent into HGB flow cell by activating for 11.4 - 14.0 sec.
- HGB flow cell is air mixed by activating SV11.
[RBC]
- RBC sample is charged by activating SV67 and SV68 for 14.4 - 15.9 sec.
- RBC sheath form is generated by activating SV70 for13.5 - 36.4 sec.
- Front flash activated by SV71 for 13.3 - 14.3 sec.
- Back flash activated by SV72 for 13.4 - 13.7 sec.
XE-2100 S/M 2-16 February 5, 2009

[IMI]
- Solution in IMI detector chamber is drained by activating SV14 for 10.2 - 11.4 sec.
- IMI detector chamber is air mixed by activating SV12.
XE-2100 S/M 2-17 February 5, 2009

XE-2100 S/M 2-18 February 5, 2009
15 - 20 Sec
- Whole blood pump ascend to rinse sample line for 17.7 - 19.7 sec. (moves slowly to prevent drop-
ping sample from the pipette tip.)
- SRV is rinsed (perimeter cleaning) by activating SV60 for 15.7 - 16.7.sec.
- Diluent is dispensed to rinse cup by activating SV56 and drained into WC1 by activating SV59.
- SLS, SIM, FBT,FFD, and RED reagent is replenished by activating SV1, 4, 5, 6, 7, and 9.
- SNR is replenished by activating SV8 for 17.5 - 18.5 sec.
- Diluent is dispensed to NRBC reaction chamber by activating SV46 for 17.2 - 17.4 sec.
[NRBC]
- NRBC sample is charged to sheath sample line by activating SV47 and SV 24 for 16.4 - 17.5 sec.
- Sheath syringe ascends for 17.5 - 22.5 sec.
- Low speed sheath form is generated by SV28 and SV20 from 15 sec and after.
- High speed sheath form is generated by SV29 and SV20 for 17.7 - 22.6 sec.
- NRBC is counted for 18.8 - 22.5 sec.
- Sheath syringe is rinsed by SV53 for 17.3 - sec.
- EPK is replenished by activating SV3 for 17.0 - 18.2 sec.
- Sheath syringe is ascend for 17.5 - 22.5 sec.
- NRBC is counted for 18.8 - 22.5 sec.
[RBC]
- RBC sample is charged by activating SV67 and SV68 for 14.4 - 15.9 sec.
XE-2100 S/M 2-19 February 5, 2009

XE-2100 S/M 2-20 February 5, 2009
20 - 25 sec.
- Whole blood pump ascend to rinse sample line for 19.7 - 21.6 sec.
- Whole blood pump descend to make air gap for 22.6 - 23.1 sec.
- Diluent is dispensed to rinse cup by activating SV56 and drained into WC1 by activating SV59
while rinse cup ascends for clean pipette outside.
- Water in the air filter is drained by activating SV55 for 23.9 - 24.0 sec.
[BASO]
- Diluent is dispensed to BASO reaction chamber by activating SV52 for 24.3 - 24.5 sec.
- Charge line is rinsed by activating SV21 and SV22.
- Sample is charged by activating SV16 and SV24.
- Sheath syringe descends for 22.6 - 24.6 for preparing BASO counting.
- Sheath syringe line is rinsed by activating SV51 for 22.5 - 23.2 sec.
- EPK is replenished by activating SV3 for 22.5 - 23.7 and 24.0 - 25.1 sec.
[RBC]
- RBC counts for 23.0 - 35.8 sec.
[IMI]
- IMI manometer is filled by activating SV 17 for 22.9 - 31.3 sec.
XE-2100 S/M 2-21 February 5, 2009

XE-2100 S/M 2-22 February 5, 2009
25 - 30 sec
[BASO]
- BASO is counted for 26.1 - 30.5 sec.
[HGB]
- HGB converts for 26.0 - 27.0 sec.
[RBC]
[IMI]
- IMI manometer is filled by activating SV 17 for 22.9 - 31.3 sec.
XE-2100 S/M 2-23 February 5, 2009

XE-2100 S/M 2-24 February 5, 2009
30 - 35 sec
[DIFF]
- Diluent is dispensed to DIFF chamber by activating SV50 for 32.1 32.3 sec.
- Charge line is rinsed by activating SV21 and 24 for 30.5 - 31.2 sec.
- DIFF Sample is charged by activating SV31 and 24 for 31.4 - 32.4 sec.
- DIFF is counted for 18.8 - 22.5 sec.
- Sheath syringe (low speed) is rinsed by SV53 for 32.2 - 32.7 sec.
- Sheath syringe (high speed) is rinsed by SV51 for 30.5 - 31.2 sec.
- EPK is replenished by activating SV3 for 30.5 - 32.1 sec 32.1 - 33.0 sec.
[RBC]
XE-2100 S/M 2-25 February 5, 2009

XE-2100 S/M 2-26 February 5, 2009
35 - 40 sec
[RET]
- Diluent is dispensed to RET reaction chamber by activating SV49 for 39.9 - 40.0 sec.
- RET sample is charged by activating SV35 and 24 for 39.2 - 40.3 sec.
- RET is counted for 43.0 - 46.2 sec.
[RBC]
- RBC sheath syringe is rinsed by activating SV 68 and 66 for 35.9 - 36.4 sec.
- ESE is replenished by activating SV2 for 35.9 - 37.9 sec.
- RBC sheath syringe descends for 35.9 - 39.0 sec.
XE-2100 S/M 2-27 February 5, 2009

XE-2100 S/M 2-28 February 5, 2009
40 - 45 sec
[RET]
- Diluent is dispensed to RET reaction chamber by activating SV49 for 39.9 - 40.0 sec.
- RET sample is charged by activating SV35 and 24 for 39.2 - 40.3 sec.
XE-2100 S/M 2-29 February 5, 2009

XE-2100 S/M 2-30 February 5, 2009
45 - 50 sec
[RET]
- Charge line is rinsed by activating SV21, 22, and 51 for 47.1 - 48.1 sec.
- RET background is counted for 46.4 - 47.1 sec to subtract the background value from the sample
value to eliminate the high background effect.
XE-2100 S/M 2-31 February 5, 2009

XE-2100 S/M 2-32 February 5, 2009
2.10 HYDRAULIC PARTS
2.10.1 Solenoid Valve Function
SV Function Located Page SV Function Located Page

No Valve No. Valve
Unit Unit
1 SLS replenishment 139 2-36 42 ESE outer float bubble 136 2-34
removal
2 ESE replenishment 139 2-36 43 WC2 (FCM sheath 136 2-34
waste) drain
3 EPK (FCM Sheath) 139 2-36 44 SIM outer float bubble 136 2-34
replenishment removal
4 FBT replenishment 139 2-36 45 WC3 (IMI waste) drain 136 2-34
5 SIM replenishment 139 2-36 46 NRBC reaction 136 2-34
chamber rinse
6 apply vacuum to 139 2-36 47 NRBC charging line 136 2-34
Reagent chambers open
7 4DL replenishment 139 2-36 48 Latex for OPT align/ 136 2-34
CELL CLEAN for
SHUTDOWN
8 SNR replenishment 139 2-36 49 RET reaction chamber 136 2-34
rinse (discrete)
9 RED replenishment 139 2-36 50 DIFF reaction chamber 136 2-34
rinse(discrete)
10 EPK (Diluent) 139 2-36 51 FCM sheath syringe 136 2-34
replenishment intense rinse
11 HGB air mixing 134 2-33 52 BASO reaction 136 2-34
chamber
rinse(discrete)
12 IMI air mixing 134 2-33 53 FCM sheath syringe 136 2-34
rinse
13 BASO air mixing 134 2-33 54 WB aspirates (OFF for 136 2-34
SRV)
14 IMI draining 134 2-33 55 FCM Sheath, RBC 137 2-34
bubble removal 2.5Kg/
cm2 drain)
15 HGB dilution DP 134 2-33 56 Dispense rinse solution 137 2-34
dispense to manual rinse cup
16 BASO charging line 134 2-33 57 WC1 drain 137 2-34
open
17 IMI count 134 2-33 58 CP rinse cup drain 137 2-34
18 RBC dilution DP 134 2-33 59 Manual rinse cup drain 137 2-34
dispense
19 BASO dilution DP 134 2-33 60 SRV perimeter rinse 137 2-34
dispense
20 FCM sheath output port 134 2-33 61 IMI (discrete) rinse 137 2-34
open
21 FCM Rinse DP drive 134 2-33 62 NRBC dye DP 138 2-35
(Rinse) dispense
22 FCM charging line 134 2-33 63 DIFF dye DP dispense 138 2-35
switch
XE-2100 S/M 2-33 February 5, 2009

SV Function Located Page SV Function Located Page
No Valve No. Valve
Unit Unit
23 SRV turn (ascend on 134 2-33 64 RET dye DP dispense 138 2-35
ON)
24 FCM charging DP drive 134 2-33 65 RBC sample chamber 133 2-33
drain
25 IMI clog removal air ON 134 2-33 66 RBC sheath syringe 133 2-33
rinse
26 IMI filling 134 2-33 67 RBC sample charging 133 2-33
27 SLS DP drive 135 2-34 68 RBC charging DP 133 2-33
activate
28 Generate low speed 135 2-34 69 RBC sheath WC drain 133 2-33
sheath
29 Generate high speed 135 2-34 70 RBC sheath generate 133 2-33
sheath
30 IMI DP drive 135 2-34 71 RBC front flash & 133 2-33
transducer bubble
removal
31 DIFF charging line 135 2-34 72 RBC back flash 133 2-33
open
32 RED DP dispense 135 2-34 73 Waste container sensor --- ---
drain
33 DIFF diluent DP 135 2-34 74 Not used --- ---
dispense
34 NRBC diluent DP 135 2-34 CP1 Apply pressure air to CP Unit ---
dispense CP needle
35 RET charging line open 135 2-34 CP2 Dispense rinse solution CP Unit ---
to CP needle
36 HGB drain 135 2-34 CP3 CP needle pierce CP Unit ---
37 RET reaction chamber 135 2-34 CP4 CP clump CP Unit ---
drain
38 DIFF reaction chamber 135 2-34 CP5 CP hand turning mixing CP Unit ---
drain
39 NRBC reaction 135 2-34 CP6 CP hand forward / CP Unit ---
chamber drain backward (ON for
forward)
40 BASO reaction 135 2-34 CP7 CP hand descend CP Unit ---
chamber drain
41 FCM flow cell air 135 2-34 CP8 CP hand ascend CP Unit ---
bubble remove
XE-2100 S/M 2-34 February 5, 2009

2.10.2 HYDRAULIC PARTS LOCATION
Left side
XE-2100 S/M 2-35 February 5, 2009

Valve Unit No.133
XE-2100 S/M 2-36 February 5, 2009

Valve Unit No. 134
Valve Unit No.135 (ECR 308F056 not applied)
Valve Unit No.135 (ECR 308F056 applied)
XE-2100 S/M 2-37 February 5, 2009

Valve Unit No.136 (ECR 308F056 not applied)
Valve Unit No.136 (ECR 308F056 applied)
Valve Unit No.137
XE-2100 S/M 2-38 February 5, 2009

Valve Unit No.138
Valve Unit No. 139
XE-2100 S/M 2-39 February 5, 2009

2.11 SRV STRUCTURE
XE-2100 S/M 2-40 February 5, 2009

XE-2100 S/M 2-41 February 5, 2009
2.12 CONVERSION TABLE OF VACUUM AND PRESSURE VALUES
Conversion formulas for vacuum and pressure values are shown as following (1) and (2). When
the value is not listed, calculate and obtain those values.
(1) Vacuum (y) in SI unit (MPa) = Vacuum (x) in conventional unit (mmHg) * 0.000133322
(2) Pressure (Y) in SI unit (MPa) = Pressure (X) in conventional unit (kg/cm2) * 0.0980665
Vacuum in Vacuum in SI Unit Pressure in Pressure in SI Unit

Conventional Unit (x) (y) Conventional Unit (X) (Y)
5 mmHg 0.0007 MPa 0.001 kg/cm2 0.0001 MPa
2
10 mmHg 0.0013 MPa 0.003 kg/cm 0.0003 MPa
20 mmHg 0.0027 MPa 0.005 kg/cm2 0.0005 MPa
2
35 mmHg 0.0047 MPa 0.01 kg/cm 0.0010 MPa
2
100 mmHg 0.0133 MPa 0.03 kg/cm 0.0029 MPa
150 mmHg 0.0200 MPa 0.05 kg/cm2 0.0049 MPa
180 mmHg 0.0240 MPa 0.07 kg/cm2 0.0069 MPa
200 mmHg 0.0267 MPa 0.1 kg/cm2 0.0098 MPa
215 mmHg 0.0287 MPa 0.2 kg/cm2 0.0196 MPa
220 mmHg 0.0293 MPa 0.25 kg/cm2 0.0245 MPa
230 mmHg 0.0307 MPa 0.27 kg/cm2 0.0265 MPa
240 mmHg 0.0320 MPa 0.300 kg/cm2 0.0294 MPa
248 mmHg 0.0331 MPa 0.301 kg/cm2 0.0295 MPa
250 mmHg 0.0333 MPa 0.33 kg/cm2 0.0324 MPa
252 mmHg 0.0336 MPa 0.35 kg/cm2 0.0343 MPa
260 mmHg 0.0347 MPa 0.380 kg/cm2 0.0373 MPa
270 mmHg 0.0360 MPa 0.400 kg/cm2 0.0392 MPa
280 mmHg 0.0373 MPa 0.43 kg/cm2 0.0422 MPa
290 mmHg 0.0387 MPa 0.45 kg/cm2 0.0441 MPa
298 mmHg 0.0397 MPa 0.47 kg/cm2 0.0461 MPa
299 mmHg 0.0399 MPa 0.497 kg/cm2 0.0487 MPa
2
300 mmHg 0.0400 MPa 0.500 kg/cm 0.0490 MPa
380 mmHg 0.0507 MPa 0.510 kg/cm2 0.0500 MPa
2
390 mmHg 0.0520 MPa 0.52 kg/cm 0.0510 MPa
2
400 mmHg 0.0533 MPa 0.53 kg/cm 0.0520 MPa
2
415 mmHg 0.0553 MPa 0.55 kg/cm 0.0539 MPa
420 mmHg 0.0560 MPa 0.57 kg/cm2 0.0559 MPa
2
440 mmHg 0.0587 MPa 0.60 kg/cm 0.0588 MPa
2
450 mmHg 0.0600 MPa 0.605 kg/cm 0.0593 MPa
2
500 mmHg 0.0667 MPa 0.62 kg/cm 0.0608 MPa
510 mmHg 0.0680 MPa 0.622 kg/cm2 0.0610 MPa
530 mmHg 0.0707 MPa 0.650 kg/cm2 0.0637 MPa
550 mmHg 0.0733 MPa 0.665 kg/cm2 0.0652 MPa
2
600 mmHg 0.0800 MPa 0.70 kg/cm 0.0686 MPa
XE-2100 S/M 2-42 February 5, 2009

Vacuum in Vacuum in SI Unit Pressure in Pressure in SI Unit
Conventional Unit (x) (y) Conventional Unit (X) (Y)
0.701 kg/cm2 0.0687 MPa
2
0.750 kg/cm 0.0735 MPa
1.000 kg/cm2 0.0981 MPa
2
1.030 kg/cm 0.1010 MPa
2
1.05 kg/cm 0.1030 MPa
2
1.1 kg/cm 0.1079 MPa
1.5 kg/cm2 0.1471 MPa
1.588 kg/cm2 0.1557 MPa
1.60 kg/cm2 0.1569 MPa
1.601 kg/cm2 0.1570 MPa
1.7 kg/cm2 0.1667 MPa
1.80 kg/cm2 0.1765 MPa
1.9 kg/cm2 0.1863 MPa
2.000 kg/cm2 0.1961 MPa
2.010 kg/cm2 0.1971 MPa
2.1 kg/cm2 0.2059 MPa
2.15 kg/cm2 0.2108 MPa
2.200 kg/cm2 0.2157 MPa
2.240 kg/cm2 0.2197 MPa
2.25 kg/cm2 0.2206 MPa
2.30 kg/cm2 0.2256 MPa
2.4 kg/cm2 0.2354 MPa
2.492 kg/cm2 0.2444 MPa
2.5 kg/cm2 0.2452 MPa
2.513 kg/cm2 0.2464 MPa
2.533 kg/cm2 0.2484 MPa
2.7 kg/cm2 0.2648 MPa
2.8 kg/cm2 0.2746 MPa
3.0 kg/cm2 0.2942 MPa
XE-2100 S/M 2-43 February 5, 2009

2.13 Hydraulic System Difference Between XE-2100 and
XE-2100 DC
2.13.1 XE-2100 Hydraulic System
2.13.2 XE-2100DC (XE-2100C) Hydraulic System

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SECTION 2 HYDRAULICS

2.1 PNEUMATIC SYSTEM........................................................................................................................1

2.2 USE OF PRESSURE AND VACUUM .................................................................................................2

2.3 HYDRAULIC SYSTEM ........................................................................................................................3

2.4 ANALYSIS PARAMETER ....................................................................................................................4

2.5 REAGENT SYSTEM ...........................................................................................................................6

2.6 ANALYSIS FLOW................................................................................................................................7

2.7 FCM DETECTOR BLOCK...................................................................................................................8

2.8 OPTICAL SYSTEM ...........................................................................................................................11

2.9 HYDRAULIC SEQUENCE DESCRIPTION .......................................................................................12

2.10 HYDRAULIC PARTS .......................................................................................................................33

2.11 SRV STRUCTURE ..........................................................................................................................40

2.12 CONVERSION TABLE OF VACUUM AND PRESSURE VALUES..................................................42

2.13 Hydraulic System Difference Between XE-2100 and XE-2100 DC ........................................44

XE-series S/M February 5, 2009

The outline of pneumatic control system is shown below.

XE-series S/M 2-1 February 5, 2009

XE-series S/M 2-2 February 5, 2009

WBC/RBC Block Diagram

RBC/HGB Block Diagram

XE-series S/M 2-3 February 5, 2009

Analysis Block RBC/PLT HGB IMI

Analysis Block WBC/RET

XE-series S/M 2-4 February 5, 2009

*3 NRBC Scattergram *4 RET Scattergram

XE-series S/M 2-5 February 5, 2009

Figure 2-2 Reaction Chamber Construction

XE-series S/M 2-6 February 5, 2009

XE-series S/M 2-7 February 5, 2009

The life of the Laser Diode is approx. 10,000 hours.

XE-series S/M 2-8 February 5, 2009

SSCL: Side SCattered peak Logarithm

Following table shows which receiver is used for each parameter.

(2) Beam spot generating system

(3) Sheath flow system

XE-series S/M 2-9 February 5, 2009

XE-series S/M 2-10 February 5, 2009

XE-2100 S/M 2-11 February 5, 2009

XE-2100 S/M 2-12 February 5, 2009

XE-2100 S/M 2-14 February 5, 2009

XE-2100 S/M 2-16 February 5, 2009

XE-2100 S/M 2-17 February 5, 2009

XE-2100 S/M 2-19 February 5, 2009

XE-2100 S/M 2-21 February 5, 2009

XE-2100 S/M 2-23 February 5, 2009

XE-2100 S/M 2-25 February 5, 2009

XE-2100 S/M 2-27 February 5, 2009

XE-2100 S/M 2-29 February 5, 2009

XE-2100 S/M 2-31 February 5, 2009

2.10.1 Solenoid Valve Function

SV Function Located Page SV Function Located Page

XE-2100 S/M 2-33 February 5, 2009

XE-2100 S/M 2-34 February 5, 2009

XE-2100 S/M 2-35 February 5, 2009

XE-2100 S/M 2-36 February 5, 2009

Valve Unit No.135 (ECR 308F056 not applied)

Valve Unit No.135 (ECR 308F056 applied)

XE-2100 S/M 2-37 February 5, 2009

Valve Unit No.136 (ECR 308F056 applied)

Valve Unit No.137

XE-2100 S/M 2-38 February 5, 2009

Valve Unit No. 139

XE-2100 S/M 2-39 February 5, 2009

3 NRBC Scattergram 4 RET Scattergram