ca RID

Determination of the C3 protein, by radial immunodifhrsion plate

Procedure 1
Measure's limit
H
i
TEST SUMMARY

containing a specific antibody will form an

Construct a curve

ir,. ctntroli. R ir4tr

plots the square of the-
precipitating ring wsrs the concentrations of
tine, with an intercept in
26 - 300 mg/dl
WASTE D]SPOSAL
ir*rno-Jorpf"x, visible as a ring around the
to the range 10-11 nrnr'sirould be obtained' ffiprofessionai bca,f
;;il il" ring diameter is direcl proportional
protdn The SamPle-values #brmined by ioterpotatioo' faborifories Fbase consslt 'eguhtirns'or
#-'""no.rriti* cf the analysed 1E aorr€ddry.
roodtirl cdrespdtds b ltle dfrrsbn lirE [l Pmced.se 2 \
Ed * tt" erd aTat)' trre sryae d dancer crrr"rt a or.re tnafPmtsl- sqze d h
*ifl-b" i^ linear p'loportbn to lhe concentralion .r".EE "o ring versus tEgraph togari[trn
;;;;;;d*; t-s), white afrer shorter period or [n itr""d"s of the controls'Tr will be
[ftuiion the square of diameter will be in a a *dfrt line onlY for low values
roortitnri. proportion to the concentration
toioceOure 2i. tn notn cases, a calibration curve
Sat?6 values aie determined
Prcctdure
by itEgorafion'
icr
PACKAGING

1x1swells
InorfO Ou constructed, using at least three
3

.ufiUtution points. However a reference table

is R d on enclosed reference Ble the
t' tt"
orovided showing the relation between any ;rir,Efi"tb" value corresponding
ring vah'e is
REFERENCES
Loncentration and the end of the procedure
."ciflatts ring diameter. The(that Mancini & coll.-lmmunocnemEtryzzes
(r 96s)
;EiEd trm ttr! control serum has b be a coll.- J. lmmunol g4 : 84 (1965)
Faney
used wery time) which should have a
corffence iimit ot O'2 mm (from the refetence
@at+"c. r*b)-
MANUFACTURER
LTA s.r.l.
REAGENTS Via Milano 15/F
-Cl the goat X'TES 20060 Bussero (Milan) ITALY
Plate: Agarose containing
TtE dfius'rd time and the reading time depend Tel: ++39 02 95409034
antiserum Cg.
qr tre concentration and the specific diffusion Fax: ++39 02 95334185
..*ir. etet 72 h the diffusion of the protein at e-mail: info@ltaonline'it
REAGENTS PREPARATION AND STORAGE lnv concentration is completed' For lower website: httP://www.ltaonline it
The olates are ready to use'
on
it is possible to read in lower
Tne ieagents are stable until expiration-date lt: (.". 36 h), however h sudt cases il is
"onoerg-ation SYTBOLS
tne AUei it preserved horizontal at 2€"C !o read-again afler 315 turs' If tle
Stabilitv afrer opening: weeks if' der fie
trc
fiJus6, is preserved well closed at 2-8'C'weeks
;;.ih
ffi-it ule l-r:
n ir P9=u-bsettE
concenhation, on tlc contrary, if the dtneter
;ifilr".t
=nii

ring shox be
ls
remeasured afler a
bE Odtb nDte
Lot of manufactuling
in"-rf.t" can be used for further 2
checking the accuracy by a control serum'
further 3/5 hours. E Code number
Storage temPerature interval
CALIBRATION T
!9I sy!l!!E9
MATERIALS REQUIRED BUr
ffitioe rule, current It-E-G6E6ieA toffioom an internal quality
this prrpose the following human
I Expiration date

laboratory instrumentation "onttof.-iot

based control sera ae avaible: A Warning, read enclosed documents

PRECAUTIONS 1C00400 Multiset multiparametric

4 x 1 ml rE Read the directions

acid Glvcoprotein, c3, c4' lgA' lgG'

@nt9_,_.1Y,"11
or"rL*rtiru components lt is suggested to
ii"i"-i
igM and Transfenin)
& Biological risk

- 13/09/2005)
ii, avoiding contact with skin Mod. 01 .06 (ver 1 '4
[r-nO]"
""t"rurfy
and swallow. TEST PERFORMA}'GE
ili"* to the general "Good
in" t"tt according
(GLP) guidelines'
((
Laboratory Practice"

PROCEDURE
ffiloPeand.leave
io al room temperature for few minutes
ro "i"na
itt"t condensed water in the wells can
"nv the wells with 5 pl of sample
I"oioi ionttort and wait it has been coropletely
"r"ootut".'Fill
before handling the plate Close the
"i""iti""
ili;;;i place it in a moist chamber' Wait the
I"irituJ-iir.ruation period (72 hours for the
orocedure 1 or 3, 18 hours for the proceoure.zJ' Methods comParison
i;;;;i"" analvsis time it is possible to put the a tetween LTA and a commercially
plates in a thermostat' product gave lhe following results on
"orno"tiion
"ruif"6fu
70 samPles:
RESULTS ]NTERPRETATION
imm, after the required period according
to Ine
C:LTA=x
cr competitor = Y
'18 hours' n=70
procedure should be followed, 72 or
y = 1,008x + 0,366 r = 0,9997