2. What is a Prokaryote? - Prokaryotes are organisms made up of cells that lack a cell nucleus or any membrane-encased organelles. This means the genetic material DNA in prokaryotes is not bound within a nucleus. In addition, the DNA is less structured in prokaryotes than in eukaryotes: in prokaryotes, DNA is a single loop while in Eukaryotes DNA is organized into chromosomes. Most prokaryotes are made up of just a single cell (unicellular) but there are a few that are made of collections of cells (multicellular). FUNCTIONS 1. NUCLEUS The nucleus contains a cell’s deoxyribonucleic acid (DNA), its genetic material. DNA contains instructions for making proteins, which controls all of the body’s activities. In the nucleus, DNA is tightly winded around histones, which are proteins, to form structures called chromosomes. The nucleus regulates which genes are expressed in the cell, which controls the cell’s activity and functioning and will be different depending on the type of cell. DNA is located in the nucleolus region of the nucleus, where ribosomes are made. The nucleus is surrounded by a nuclear envelope (also called nuclear membrane), which separates it from the rest of the cell. The nucleus also regulates the growth and division of the cell. When the cell is preparing to divide during mitosis, the chromosomes in the nucleus duplicate and separate, and two daughter cells are formed. Organelles called centrosomes help organize DNA during cell division. Cells usually have one nucleus each. 2. RIBOSOMES Ribosomes are where proteins are synthesized. They are found within all cells, including animal cells. In the nucleus, a sequence of DNA that codes for a specific protein is copied onto a complementary messenger RNA (mRNA) chain. The mRNA chain travels to the ribosome via transfer RNA (tRNA), and its sequence is used to determine the correct placement of amino acids in a chain that makes up the protein. In animal cells, ribosomes can be found freely in a cell’s cytoplasm, or attached to membranes of the endoplasmic reticulum. 3. ENDOPLASMIC RETICULUM The endoplasmic reticulum (ER) is a network of membranous sacs called cisternae that branches off from the outer nuclear membrane. It modifies and transports proteins that are made by ribosomes. There are two kinds of endoplasmic reticulum, smooth and rough. Rough ER has ribosomes attached. Smooth ER does not have ribosomes attached and has functions in making lipids and steroid hormones and removing toxic substances. 4. VESICLES Vesicles are small spheres of a lipid bilayer, which also makes up the cell’s outer membrane. They are used for transporting molecules throughout the cell from one organelle to another and are also involved in metabolism. Specialized vesicles called lysosomes contain enzymes that digest large molecules like carbohydrates, lipids, and proteins into smaller ones so that they can be used by the cell. 5. GOLGI APPARATUS The Golgi apparatus, also called the Golgi complex or Golgi body, is also made up of cisternae, but the cisternae are not interconnected like those of the ER. The Golgi apparatus receives proteins from the ER and folds, sorts, and packages these proteins into vesicles. 6. MITOCHONDRIA The process of cellular respiration occurs in the mitochondria. During this process, sugars and fats are broken down and energy is released in the form of adenosine triphosphate (ATP). ATP powers all cellular processes, and mitochondria produce a cell’s ATP, so mitochondria are commonly known as “the powerhouse of the cell”. 7. CYTOSOL The cytosol is the liquid contained within cells. Cytosol and all the organelles within it, except for the nucleus, are collectively referred to as a cell’s cytoplasm. This solution is mostly made of water, but also contains ions like potassium, proteins, and small molecules. 8. CYTOSKELETON The cytoskeleton is a network of filaments and tubules found throughout the cytoplasm of the cell. It has many functions: it gives the cell shape, provides strength, stabilizes tissues, anchors organelles within the cell, and has a role in cell signaling. There are three types of cytoskeletal filaments: microfilaments, microtubules, and intermediate filaments. Microfilaments are the smallest, while microtubules are the biggest. 9. CELL MEMBRANE The cell membrane surrounds the entire cell. Animal cells only have a cell membrane; they do not have a cell wall as plant cells do. The cell membrane is a double layer made up of phospholipids. Phospholipids are molecules with a phosphate group head attached to glycerol and two fatty acid tails. They spontaneously form double membranes in water due to the hydrophilic properties of the head and hydrophobic properties of the tails. The cell membrane is selectively permeable, meaning it only allows certain molecules through. Oxygen and carbon dioxide pass through easily, while larger or charged molecules must go through a special channel in the membrane. This maintains homeostasis within the cell. 10.