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:IEC
rlNSTRUCTION
MANUAL
IM-3411
Revision 2

·- r QUALITY
IEC
\
CENTRIFU&ES
SINCE 1901.

INTERNATIONAL EQUIPMENT COMPANY

IEC 300 SECOND AVENUE, NEEDHAM HEIGHTS, MA 02194 U.S.A.
TEL . (781) 449-8060 • TOLL FREE: (800) 843-1113 • FAX: (781) 444-6743
 Table of Contents

INTRODUCTION ·· ····· ········· ················· ················ ···················· ······· ··················· 2

1
1.1 General ........ ......... .... .......... .... ..... ........... .... ............................................... 2
1.2 Hematocrit Test (using 275 rotor) ... ...... .............................. .. .·..... .............. 2
2 INSTALLATION ................ ..................................................... :......... :...... .... ... .... 4
3 OPERATION ............................................................................................. ......... 5
3.1 Warnings and Cautions ....................................................... :...................... 5
3.2 Rotor Installation ........................................................................................ 6
3.3 Starting And Stopping A Run .................................................................... 8
3.4 Rotor Removal ........................................................................................... 8
4 MAINTENANCE ............ ..................................................................................... 9
4. 1 Cleaning ..................................................................................................... 9
4.2 General ...................................................................._.............................·..... 9
4.3 Brush Replacement ..................................... : ............................................. 9
4.4 Warranty .................................................................................................... 10
4.5 Condition of Returned Equipment ............................................................. 10
5 SPECIFICATIONS ............................................................................................. 11
6 DRAWINGS ....................................................................................................... 12

IMPORTANT

This manual may not contain information on all changes that have occurred to the subject
instrument since the manual issue date. It was prepared for use by IEC authorized factory-trained
service or dealer personnel who are kept current through a program of service letters and bulletins
and training seminars.

This manual contains warnings against operating procedures which could result in an accident
and/or personal injury. It also contains cautions against procedures which could result in
damage to your centrifuge or accessory equipment. Read this manual thoroughly before
operating or servicing this centrifuge.

Micro MB Instruction Manual 1

I 1 INTRODUCTION
I
I

1.1 General
. . rtable benchtop centrifuge designed specifically for
The IEC M1~ro Mh B is t ~ centrifuge (for in vitro diagnostic use) or a
use as a micro ema . • ed
microcentrifuge, depending on which rotor is us .

There are six models:

Cat No 3413 for 120 V' 50/60 Hz (No Rotor)
• C t. No. 3414 for 240 V, 50/60 Hz (No Rotor)
• c:t: No: 3615 for 120 V, 50/60 Hz (837 Rotor)
• Cat. No. 3616 for 240 V, 50/60 Hz (837 Rotor)
• Cat. No. 3411 for 120 V' 50/60 Hz (275 Rotor)
• Cat. No. 3412 for 240 V, 50/60 Hz (275 Rotor)
•
The 837 rotor is a 45° foced angle microtube rotor. The 275 rotor is a ~4
place micro hematocrit rotor which requires a separate reader for readmg the
hematocrit test (IEC Reader No. 2201 ).

The Micro MB is a compact and quiet, single speed centrifuge that utilizes a
power interrupt switch, so when the cover is opened during rotation, ~wer to
the motor is removed. It has a momentary spm feature and a fast acting
manual brake.

1"2 Hematocrit Test (using 275 rotor)

The micro method for detennination of hematocrit has been widely used in
clinical laboratories for many years. The results of micro hematocrit
determinations are recognized as being fully equivalent to the results of
macro detenninations. The hematocrit is a quantitive test of the red cells and
the plasma content of a micro sample of human blood.

The usual micro technique for determining hematocrit utilizes a small glass
capillary tube, internally coated with an anticoagulant (heparin). This
anticoagulant is non-readive with the separation of blood cells and plasma
and is placed inside of the capillary tube as a catalytic preservative. The
tube is partially filled with blood obtained from a finger puncture. One end of
the tube is sealed with plastic clay. The capillary tube is placed in a micro
hematocrit centrifuge where it is spun at high speed for about 5 minutes.

2 Micro MB Instruction Manual

 After centrifugation, tha blood sample appears into two major fractions - a
column of packed red calla a nd a column of clear plasma; with the small
fraction of white cells and platelets between the major fractions .

Th e hematocrtt is defined as th e ratio of the volume of packed red cells to the

volume of the origina l sample, multiplied by 100. Since the capillary _tube is
of uniform bore, the hematocrit may be easily determined by measuring the
length of the column of packed red cells against the overall length of the
blood sample. The hematocrit value may then be redefined as follows :
'
HEMATOCAIT = length of column of packed red cells X 100
overall length of blood sample

Usually the two lengths are measured with the aid of special mechanical
reading devices, such as the IEC reader (Cat. No. 2201 ). Such devices
present the final hematocrit value without requiring any computation by the
operator. ·

Micro MB Instruction Manual

3
 2 INSTALLATION

The Micro MB, as shipped from the factory, is ready for use. Verity that .
voltage at your site is appropriate for the model you received. The centrifuge
is equipped with a universal motor for use on 120 or 240 volts, 50/60 Hz.
The voltage requirements are shown on the centrifuge's data plate (located
on bottom plate of centrifuge).

Warning: This centrifuge is equipped with a three-prong power

cord which requires a grounded power supply. If your
facility does not have grounded power outlets, proper
grounding of the cabinet must be otherwise provided.

4
Micro MB Instruction Manual
 3 OPERATION

3.1 Warnings and Cautions

Warnings To Avoid Electric Shock:

• Plug the power cord into a grounded outlet.

• Never remove the grounding prong from the power plug, or use any
adapter which does not complete the grounding circuit.

• Always unplug the power cord before attempting to clean or service

the centrifuge.

• Do not open the cover before the rotor comes to a complete stop.

Cautions • For proper operation, always load the rotor symmetrically with a ful\
(or at least paired) set of tubes. Verify that each tube is filled
equally (by weight or at least visually) to its corresponding opposite.

• Do not block the vents because airflow will be restricted and

overheating may take place.

Micro MB Instruction Manual

5
I
'
I ■
3.2 Actor Installation
I
Open the latch and lift the cover of the centrifuge. Remove any locking nut
that may be located on the head adapter (threaded s~ft) . ~lace a rotor (275
or 837) onto the adapter making sure it engages the drive pms of the
I
adapter. To do so, place one hand on the rotor and one hand on the shaft.
Rotate the rotor and the shaft in opposite directions until you feel the rotor
drop down onto the drive pins. Install the rotor locking nut onto the threaded I
shaft and tighten it using the spanner wrench.

275 Rotor When using a 275 rotor it is first necessary to install the 1505 cushion. Place
it so that it lines the inner side of the flange of the rotor making a smooth
butt-joint with the ends. (It may be necessary to cut off a short piece of
excess length to obtain the correct fit.) A thin layer of vacuum grease (IEC
Part No. 2084) on the outer side of the cushion will provide sufficient
adhesion to hold it in place and will facilitate replacement. Occasional
shifting of the cushion will be necessary as it becomes punctured with use.

When loading hematocrit tubes into the 275 rotor, be sure that all tubes are
touching the rubber cushion, and that the other ends of the tubes do not
protrude into the center recess in the rotor where the locking nut fits.

Place the rotor cover onto the adapter and tighten the knob onto the rotor
until it is finger tight.

837 Rotor
Adapters c~me with each 837 rotor to allow smaller microtubes to be used.
The rotor directly accepts 11 mm diameter, 1.5 or 2.0 ml plastic microtubes
~se the black plastic ad~p~ers (IEC Part No. 5763) when using 8 mm ·
d,a_meter ~-0 (Becton Dickinson) Microtainers and 0.5 ml tubes Use the
white plastic adapters (IEC Part No. 5764) when using 6 mm di~meter O 4
and 0.25 ml tubes. ·

Balance
lndstall equ~I (b~ weight or by visual inspection) samples (including
a apters) ,n pairs across from each oth . . any
tubes may be used in the same run e_r ,n a rotor. Different adapters or
rotor as though they were the only prov,,de~ they are balanced around the
samp es in the rotor.

Micro MB Instruction Manual

 ~ Rotor Locking t,lut ·

I I I

Rotor

Head Adapter

~ p a n n e r Wrench

Rotor Installation

Micro MB Instruction Manual 7

 I

3.3 Starting And Stopping A Run

I
Plug the centrifuge in. Close and latch the cover. The centrifuge ~n be run
in a Timed or Untimed Mode. To run in the Timed Mode, tum the t~mer knob
clockwise to the desired setting. The run will start as soon as the timer knob
is turned clockwise. Select a time up to 15 minutes in 30 second increments.
If the desired run time is less than 5 minutes, turn the timer knob clocl<Wise to
the 5 minute mark and then back to the desired time. Changing the timer
knob setting during a run affects the run in progress. When the timer knob
returns to the o minute position, a signal bell sounds and the rotor coasts to a

stop.
To run in an Untimed Mode, set the timer knob to o minutes and press and
hold the Momentary Spin button. The run will start as soon as this button is
pressed, and will end as soon as it is released.

In either Mode, if faster stopping is desired, hold the brake switch down until
the rotor has almost come to rest (approximately 5 seconds) and release the
brake switch. The rotor can be seen through the top vent in the cover of the
centrifuge.

Note: The rotor will reverse direction of rotation if the brake switch is held
down after the rotor stops.

Warning: Do not open the cover before the rotor comes to a

complete stop.

Note: The powe~ will be interrupted and the brake switch will not function if
the cover rs opened. .

Once the rotor has come to a complete stop, unlatch and lift the cover.

~it Rotor Removal

To remove the rotor, use the spanner wrench
the locking nut(s) and lift the rotor off f th (IEC Part No. 1883) to loosen
0 e motor shaft.

8
Micro MB Instruction Manual
I 1
4 MAINTENANCE i
I
'
4.1 Cleaning
eb .~ r spilled sample to avoid .
Keep the centrifuge chamber free of d "~essories after each use or daily •
excessive vibration. Clean the r?tor and a Th 275 rotor will corrode when
Do not use caustic or acid cleaning agents. e
exposed to sodium or chlorine ions.

4.2 General
The Micro MB motor requires no lubrication. The motor bearings are sealed ,
pennanently lubricated bearings.

The Micro MB requires no calibration. Verification ~f spe~d is recommended

every six months. The minimum speed of the centrifuge 1s 11,600 RPM.

If the centrifuge does not start, check that the cover switch is_ engaged when
the cover is latched. If it does not engage, the latch can be tightened by
adjusting the screw inside the latch assembly. When properly adjusted, a
closed cover will compress the gasket slightly and the latch will be snug. If
the latch is snug but a gap still exists between the cover and the gasket, the
gasket must be replaced.

4.3 Brush Replacement

The motor brushes should be inspected every 3-4 months under nonnal use,
and more often with heavy use. To do this, unplug the centrifuge, remove
the rotor, and place the unit on a benchtop upside down. Remove the three
screws from the baseplate and remove the baseplate. Unscrew the motor
brush caps and remove the brushes. If the carbon portion of the· brush is 1/4
inch or less, replace the brushes. When replacing the brushes, spread the
metal clip on the end of the brush spring slightly, so that it will stay in the
brush holder until the cap is screwed in. Order a pair of replacement brushes
as IEC Part No. 1840. (A pair of brush caps is IEC Part No. 30895)

Micro MB Instruction Manual 9

I -I
I
W~rranty 'I
IEC wants you to be satisfied with the q uality of your Micro MB c entrifuge .
·11 ·
W e warrantee your IEC centrifuge for one y ear . W e_wt r~pa•r or r"'l"'
Anlace a I
product that fails , within this period from the date of its delrvery , due to
defects in material and workmanship, and w e will ship you the repaired
product or its replacement at our expense . You must use IEC approved
accessories and genuine IEC spare parts. This warranty does ~ ~ to
any instrument that has been abused or repaired without authoriza\1.o n.

THE FOREGOING OBLIGATIONS ARE IN LIEU OF ALL OTHER

OBLIGATIONS AND LIABILITIES INCLUDING NEGLIGENCE, AND ALL
WARRANTIES, OF MERCHANTABILITY OR OTHERWISE, EXPRESSED
OR IMPLIED IN FACT OR BY LAW. THE FOREGOING STATES OUR
ENTIRE AND EXCLUSIVE LIABILITY, AND BUYER' S EXCLUSIVE
REMEDY, FOR ANY CLAIM OR DAMAGES IN CONNECTION WITH THE
SALE OR FURNISHING OF GOODS OR PARTS , THEIR DESIGN ,
SUITABILITY FOR USE, INSTALLATION, OR OPERATION. IEC WILL IN
NO EVENT BE LIABLE FOR ANY SPECIAL OR CONSEQUENTIAL
DAMAGES WHATSOEVER, AND OUR LIABILITY UNDER NO
CIRCUMSTANCES WILL EXCEED THE PURCHASE PRICE FOR THE
GOODS FOR WHICH LIABILITY IS CLAIMED.

~
0
@ ~ ~&lldition of Returned Equipment

Befo_re returning equipment to IEC, you must contact IEC or your dealer and
receive a r?tum goods authorization (RGA). All returned units must be
~eco~tamInated,_ free of radioactivity, and free of hazardous and
1nfect1ou~ m~ter1als. The RGA paperwork includes a Certificate of
Decontamma!1on for you to sign indicating that you have performed these
step~. IEC will not accept the shipment until this signed certificate·
receNed. is

You must prepay transportation to the service depot.

10 Micro MB Instruction Manual

 5 SPECIFICATIONS

Rotation Speed 11 ,600 RPM minimum

14,000 RPM maximum

Relative Centrifugal Force 13,200 xg (275 rotor) minimum

8,700 xg (837 rotor) minimum
19,200 xg (275 rotor) maximum
12,700 xg (837 rotor) maximum

Acceleration Less than 1O seconds

Deceleration Less than 5 seconds (brake)
Approx. 45 seconds (coast)

· Capacity 12 x 2.0 ml (837 rotor)

24 x Hematocrit Tubes (275 rotor)

Timer
Range O to 15 minutes
Increments 30 seconds
Accuracy ± 5% full scale

Power Requirement 120 VAC ± 10%, 50/60 Hz

(Cat. No. 3411, 3613, 3615)
240 VAC ± 10%, 50/60 Hz
(Cat. No. 3412, 3614, 3616)

Power Consumption 140 Watts (operating)

Heat Output (typical) 480 Btu/hr

Height
Cover Closed: 229 mm ( 9 in.)
Cover Opened: 457 mm (18 in.)
Width 254 mm (10 in.)
Depth 279 mm (11 in.)

Net Weight 9.1 kg (20 lbs.)

Shipping Weight 10.9 kg (24 lbs.)

Specifications Subject To Change Without Notice

Micro MB Instruction Manual 11

 DRAWINGS
I
6
I

Micro MB Assy · Parts Lis

Description
t (Refer to Micro
MB ASSY. Drawing)

izml
2._4(N
49780
'
item No. 49780 41313
1 Base 41313 49793
Brake Resistor
3
Harness - Power
49793 49794
4 49794 33790
Harness _ Brake Res.
5 33790 44891
7 Ground Lead 44890
8 Motor 7915 7915
9 Head Adapter 49784 49784
10 Switch (Latch) 1511 1511
11 Gasket (Cover) 30898 30898
13 Stud 9660 9660
14 Grommet 30889 30889
15 Screen 1883 1883
16 Spanner Wrench 30901 30901
17 Coupling
38805 46759
18 Power Cord
7907 7907
19 Bead Chain
46618 46618
20 Foot
30888 30888
21 Washer
Base Plate ~0877 30877
22
Timer 768 768
23
Screw 65455 65455
24
25 Spacer 30882 30882
26 Latch Pin 30887 30887
27 Latch 30878 30878
28 Switch (Spin) 49789 49789
29 Cover 49771B 49771B
30 Front Panel 49788 49788
31 Switch (Brake) 9942 9942
32 Timer Knob 30839 30839
33 'S' Hook 30902 30902
34 Liner 42991 42991
35 Insulation 31675 31675
37 Motor Dampener 30992 30992
38 Vinyl Tip 45390 45390
39 Knurled Nut 42011 42011
40 Vent Screen 30900 30900
43 Washer 49224 49224
66 Label 50012 50012
70 Label 46625 46625

12 Micro MB Instruction Manual

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Micro MB Assy. Drawing

Micro MB Instruction Manual 13

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Micro MB Instruction. Manual

14