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Bioluminescent Ecoli
Bioluminescent Ecoli
1
Photo Gallery
Figure 1.1 & 1.2. Nutrient Agar Figure 4.1 & 4.2. Ice bath and heat shock
Preparation. of vials.
2
Data and Observations E. Interpretation
A. LB Plate All plates showed almost identical results as
predicted by the Bio-Rad Kit Manual.
The bacteria on the Luria-Bertani (LB) Plate
External factors must have affected the
grew as normal. Regular colonies were found
plates, as Set D, the LB/Amp/Arab + pGLO
on the plate after the incubation period. This
plates did not glow as prominently as
was expected as the plate was not modified in
expected.
any way by any substance except the agar.
Conclusion
B. LB|Amp
When the bacteria is exposed to the plasmid
On the plate with ampicillin, no bacteria
pGLO, the antibiotic ampicillin so that it can
grew, and like the past plate, this was also to
adopt it, and the activator arabinose, the
be expected. Ampicillin, being an antibiotic
bacterial colony will then glow. However,
for the bacteria, most likely killed .2all of it
some problems were encountered in the
on the plate before the bacteria could grow.
execution of this experiment, and that may
C. LB|Amp + pGLO have affected its results.
References
Bacterial transformation. (2007, November 16). Retrieved January 30, 2018, from
https://www.sciencelearn.org.nz/resources/2032-bacterial-transformation