GRAM POSITIVE COCCI

POSITIVE NEGATIVE

Staphylococci
Gram Stain: Gram (+) cocci in cluster
BAP: Pin- head, opaque, cream, yellow, white
Screening Test: _______________________
Confirmatory Test: ____________________

POSITIVE NEGATIVE

COAGULASE NEGATIVE Staphylococci

___________________________
Motility: _____________________
Colony on BAP: _______________________
Vogel- Johnson: _______________________
VIRULENCE FACTORS:
Protein A: ____________________________
Enterotoxin: __________________________
Staphylokinase: _______________________
Hyaluronidase: ________________________
Gelatinase: ___________________________
OTHER TESTS:
Mannitol Fementation Test: ______________
DNAse Hydrolysis Test: _________________
LATEX Agglutination Test: ______________
DISEASES
Osteomyelitis
Scalded Skin Syndrome
Toxic Shock Syndrome
Sty, Carbuncles, Furuncles
SENSITIVE RESISTANT
_____________________________
Motility: _____________________
Skin Normal Flora and Blood Culture
Contaminant
VIRULENCE FACTOR:
Biofilms: ________________________
OTHER TEST:
Mannitol Fementation Test: ______________
DISEASES
Subacute Bacterial Endocarditis
Prosthetic Valve Endocarditis

_____________________________
Motility: _____________________
Colony: ______________________
VIRULENCE FACTOR:
Adherence to urothelial cells
Lipoteichoic Acid
Hemagglutinin that binds to fibronectin
Hemolysin
Extracellular Slime
OTHER TEST:
Mannitol Fementation Test: ______________
DISEASES
UTI
Pyelonephritis
Cystitis
 MICROCOCCUS vs. STAPHYLOCOCCUS

CATALASE TEST
Micrococcus
- Motility:
____________
- Except: POSITIVE NEGATIVE
_____________ Staphylococcus
- Colony: aureus
_____________ RESISTANT SENSITIVE
-
- Media:
______________
SENSITIVE RESISTANT

Streptococci
Catalase Test:
Gram Stain: Gram (+) cocci in chains
Motility: _____________________________
Colony: Pin-point colony with large zone of
hemolysis

Beta- Hemolysis Alpha- Hemolysis Gamma- Hemolysis

- Complete hemolysis - Incomplete hemolysis - No hemolysis
- Clearing of medium - Green to brown color - No change in color

GAMMA- HEMOLYTIC STREPTOCOCCI

(GROUP D SPTRETOCOCCI)

Enterococcus Non- Enterococcus

- Enterococcus faecalis - Streptococcus bovis:
- Enterococcus faecium
- Enerococcus durans BILE ESCULIN - Streptococcus equinus

LABORATORY DIAGNOSIS: GROWTH AT 45oC LABORATORY DIAGNOSIS:

- 6.5% NaCl: ___________ - 6.5% NaCl: ___________
- PYR: ______________ - PYR: ______________
- Hippurate HOH: ________ - Hippurate HOH: ________
- PENICILLIN: ___________ - PENICILLIN: ___________
- Growth at 10oC: __________ - Growth at 10oC: __________
 BETA- HEMOLYTIC STREPTOCOCCI

SENSITIVE RESISTANT

Streptococcus pyogenes POSITIVE

- Lancefield: __________
- Motility: ____________ NEGATIVE
- VIRULENCE:
- M PROTEIN: capsule Streptococcus agalactiae
- Exotoxin - Lancefield: __________
- Streptokinase: ________ - Motility: ____________ SENSITIVE
- Hyaluronidase: _______ - Media: ______________
- Streptolysin O: - DISEASES:
____________________ - Pneumonia, Meningitis,
Sepsis GROUP C, F, G
- Streptolysin S: - Motility: ____________
___________________ - OTHER TESTS:
- SXT: ______________ - DISEASES:
- DISEASES: - Pneumonia, Cellulitis,
- Scarlet Fever, - Hippurate HOH:
____________________ Pharyngitis
Erysipelas, Puerperal - NAMES:
Sepsis, Impetigo - GROUP C:
- OTHER TESTS:
- PYR: ______________
- SXT: ______________ - GROUP F, G:

ALPHA- HEMOLYTIC STREPTOCOCCI

Doubtful screening test (9-13mm)

POSITIVE NEGATIVE
CONFIR MATORY TEST

Streptococcus pneumoniae
- Motility: ____________
- Colony: _______________ Viridans Streptococci
- VIRULENCE: - SPECIES
- Capsule - Streptococcus sanguis:
- Streptokinase: ________ -
- CHARACTERISTIC: -
- Streptococcus mutans:
- DISEASES -
- Otitis Media, Lobar - OTHER ASSOCIATED
Pneumonia DISEASE:
- Agent of Subacute bacterial
- SKIN TEST: _____________ Endocarditis with damage
heart valves
 OTHER SPECIAL STREPTOCCI SPECIES

VANCOMYCIN: RESISTANT

NUTRITIONAL VARIANT
STREPTOCOCCI
- SPECIES
o Abiotrophia spp
o Granulicatella

- When mixed around colonies of S.

aureus, they satellite around the
colonies (Staphylococcus spp are
pyridoxal producers) POSITIVE NEGATIVE

NEGATIVE POSITIVE

Pediococcus Leuconostoc
- Motility: ______________ - Motility: ______________
- Aerobic to microaerophilic - found in plants, dairy products, and
microorganisms that divide in foods
two planes to form tetrads or - Normal flora of GIT
large clumps of cells
- Wine Fermentation
 GRAM POSITIVE BACILLI

YES SPORE FORMATION NO

AEROBIC OXYGEN ENVIRONMENT ANAEROBIC YES BRANCHING NO

YES ACID FAST NO

YES CATALASE NO

YES MOTILITY NO
BILE ESCULIN

YES H2S NO
 SPORE FORMING GRAM POSITIVE BACILLI

AEROBIC OXYGEN ENVIRONMENT ANAEROBIC

YES NO NO YES

Bacillus cereus Bacillus anthracis Clostridium perfringes YES NO

Fried rice bacillus Colony:________ Other:
Food poisoning ____________________ Box Car Bacillus
Diarrheal Type:________ VIRULENCE: Important Characteristic: Clostridium botulinum YES NO
____________________ ___________________ _____________________ Canned good bacillus
Emetic Type: _________ Stain: _______________ VIRULENCE: _________ Most potent toxin Clostridium difficile
____________________ DISEASES: DISEASES: VIRULENCE: Clindamycin associated
VIRULENCE: ________ Malignant Pustule: Gas gangrene: Botulinum toxin: pseudomembranous
OTHER TESTS: _____________ Food Poisoning: enterolitis
Hemolysis: Woolsorter: Necrotic Enteritis: Neurotoxin Media: _______________
Growth on 45oC: _______________ OTHER TESTS DISEASES: TESTS:
Penicillin G: Gastroenteritis Lecithinase: Wound botulism EIA:
Gelatin HOH: Serologic Test: Floppy Baby Syndrome
Definitive Test: Reverse CAMP test: SIDS; Crib Death Gold Standard:
OTHER TESTS OTHER TESTS
OTHER BACILLUS SPP. Hemolysis: Lipase: Lecithinase: OTHER TESTS
Growth on 45oC: Lactose: Lactose: Lecithinase:
Penicillin G: Glucose: Glucose: Lactose:
Bacillus subtilis Gelatin HOH:
Motility: ____________
Clostridium tetani
Hemolysis: ___________
Tennis racket
- Quality Control
Assacharolytic
for Sterilization
Virulence: _____________
- Eye infection in
Bacillus stearothermophilus DISEASES:
heroin addicts
Motility: ____________ Spastic paralysis, Lockjaw
:
- Flat sour spoilage TESTS:
- GEOBACILLUS Clinical Findings
: Gram Stain
OTHER TESTS
Lecithinase:
Lactose:
 NON- SPORE FORMING GRAM POSITIVE BACILLI

YES BRANCHING NO

YES NO
YES NO

Nocardia
N. asteroids Actinomyces israelli
YES NO
N. farcinica YES NO
N. asiatica
N. vinaceae
N. mexicana Erysiphilothrix
rhusiopathiae
Listeria monocytogenes

NO YES Lactobacillus

Corynebacterium diphtheriae Gardnerella vaginalis

NEGATIVE VARIABLE

Corynebacterium ulcerans Corynebacterium

pseudotuberculosis
 GRAM POSITIVE DIPLOCOCCI

YES NO

Neisseria gonorrhoeae YES NO

Oxidase:
Catalase:
DNAse: Moraxella catarrhalis
ONPG
CHO UTILIZATION TESTS YES NO
Oxidase:
Glucose: Catalase:
Maltose: Superoxol:
Lactose: ONPG:
YES
CHO UTILIZATION TESTS
Glucose:
Maltose:
Lactose:

Neisseria lactamica Neisseria meningitidis

Oxidase: Oxidase:
Catalase: Catalase:
Superoxol: Superoxol:
CHO UTILIZATION TESTS CHO UTILIZATION TESTS
Glucose: Glucose:
Maltose: Maltose:
 ENTEROBACTERIACEAE

RAPID LACTOSE FERMENTER LATE LACTOSE FERMENTER

NO MOTILITY YES K/K LYSINE K/A

________________ +,+,-,- IMViC -,-,+,+ ________________ NO INDOLE YES

TSI: A/A ________________ ________________ TSI: A/A
LIA: K/K LIA: K/K
LOA: +,-,- TSI: A/A TSI: A/A ONPG: Positive
IMViC: -,-,+,+ LIA: K/K LIA: K/K IMViC: -,+,-,+
Malonate: Positive LOA: +,+,- ONPG: Positive Urease: Negative
ONPG: Positive
________________
YES INDOLE NO LOA
Malonate: Positive
+,+,- IMViC: -,+,-,+
_____________ YES VP NO Enterobacter aerogenes
Urease: Negative
UREASE Enterobacter gergoviae
LIA: K/K LIA: K/A
Hafnia alvei
VP: Positive
Urease: Positive -,+,+ YES MALONATE NO
Enterobacter cloacae
YES LYSINE DECARBOXYLASE NO Enterobacter sakazakii
-,-,-
_____________ Pantoea agglomerans
_____________
LIA: K/K
Indole: ________________
_____________ LIA: K/A
Indole: ________________
IMViC: +,+,-,+
LIA: K/K VP: Urease: Negative Indole: Positive
Indole: Urease:
VP:
Urease:
 ENTEROBACTERIACEAE

NON LACTOSE FERMENTER

NEGATIVE METHYL RED POSITIVE

NO MOTILITY YES
YES PAD NO
YES ARABINOSE NO
NO MANNITOL YES
YES H2S NO
_____________ _____________
_____________ YES ONPG NO
Red pigment Red Prodigiosin YES CITRATE NO
DNAse: ONPG: Positive YES INDOLE NO
Lipase: _____________ _____________
Citrate: _____________ ______________

_____________
_____________ _____________

_____________
POSITIVE
CITRATE _____________ NEGATIVE
_____________ NEGATIVE
INDOLE
_____________ POSITIVE POSITIVE
H2S

_____________ POSITIVE NEGATIVE

_____________
POSITIVE ODC
NEGATIVE MOTILITY AT 25oC NEGATIVE
_____________