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LIPIDS
Lipids are a dissimilar group of biological compounds, but they are poised of a chain of hydrocarbons, ending
with groups of bonded oxygen and hydrogen and share the general property of being at least partially not water
soluble. They are essential to all forms of life and are the key constituent of every cell membrane on Earth. Some
of key properties of lipids include energy storage for animals and other organisms while others are described
below:
1. Solubility: Lipids are soluble in non-polar solvent (ether, chloroform, benzene etc) but insoluble in
water.
2. Consistency: They are colorless, odorless and tasteless. Being lighter than water they have a specific
gravity of 0.86. The consistency of lipids depends upon the presence of saturated and/or unsaturated
fatty acids.
3. Hydrolysis: It is brought about in presence of acids or alkalis under the activity of enzyme lipases. Acid
hydrolysis results in the formation of glycerol and long chain of fatty acid whereas alkaline hydrolysis of
fats results in the formation of sodium or potassium salts of fatty acids called as Soaps and the process is
called as saponification.
4. Hydrogenation: The conversion of liquid vegetable oil into solid vegetable ghee by reacting with
5. Emulsification: When fats or oils are rubbed with water , the large molecules of lipids breaked into
smaller ones forming the emulsion and the process is called emulsification.
6. Rancidity: When lipids are exposed to atmosphere (heat, light, air, moisture) for more than 30 days, an
unusual and undesirable odour is developed. Such types of lipids are called rancid lipids and the
phenomenon is termed as Rancidity. Rancidity is of two types: (a) Hydrolytic and (b) Oxidative
a. Hydrolytic Rancidity: This type of phenomenon occurs due to liberation of volatile acids during
b. Oxidative rancidity: When a part of unsaturated fatty acid oxidized to form aldehyde ketone, the
at one end and hydrophobic at the other. That is why soaps made by processing accustomed lipids soften in
water and emulsifies lipids. Cell membranes are made from phospholipids bilayer with hydrophobic ends facing
the cell's externally and internally but the hydrophilic ends in front of each other within the membrane. In most
of the lipids, polar hydrophilic areas are veiled and do not dissolve in water at all. Among fats and oils, the lone
unswerving difference between fats and oils is melting temperature as both the types of triglycerides contains
Besides the frequent fatty acids with unsaturated or saturated acyl chains, chemically several fatty acids are
customized by hydrocarbon chain substituent e.g. the tidy duck gland secretes 10 carbons long fatty acid with
methyl groups on carbons 2, 4, 6, and 8 replaced with one of the hydrogens. Few bacteria make FAs with a
methyl group on the last but one carbon from the acidic group. Other bacteria integrate a cyclopropane ring in
the vicinity of the acyl chain. Mycobacterium tuberculosis synthesizes α-mycolic acids, a member of
cyclopropane-containing fatty acids. Monohydroxyl acids are another frequent component of a hydroxyl group,
present in both animals and plants in minuscule quantity, but they are ubiquitous in bacteria.
The chaste FAs form crystals set up of stacked layers of molecules. The thickness of each layer of two extended
molecules arranged in such a way that the water loving (hydrophilic) carboxylic acid groups shape the two faces
and the water-fearing (hydrophobic) chains outline the inner core. In case of specific FAs, the specifics of the
molecular packing might differ in forming different crystal configuration termed polymorphs. The SFAs of
biological significance have melting temperatures exceeding 27° C (81° F) and rise with escalating hydrocarbon
chain length. Polyunsaturated and monounsaturated molecules liquefy at considerably lower temperatures than
their saturated moiety. In most of the biological molecules, the melting temperatures becomes lowest when the
C=C are positioned in close proximity to the centre of the hydrocarbon chain. At room temperature, these
molecules form viscous liquids and the hydrophobic nature of the majority of FAs surpasses the hydrophilic
character of the carboxylic acid, creating the aqueous solubility of these molecules very squat. At 77° F (25° C),
the solubility of FA is 3 × 10−6 per gram of solution and the aqueous solubility lessens rampantly with the
accumulation of each carbon units to the hydrocarbon chain. This association imitates the energy necessary to
transmit the molecule to water from a pure hydrocarbon solvent. With every CH2 group, for example, additional
energy is obligatory to organize water molecules of FA in the region of the hydrocarbon chain resulting in the
hydrophobic effect.
The carboxylate group separates a positively charged hydrogen ion in pure water to a very small degree thus
The negative charge bearing carboxylate ion is more polar than the undissociated acid. RCOOH can be changed
to RCOO− with the addition of equal number of base molecules (e.g. NaOH). In fact, it reinstates the Na + with
H+ to provide the FA salt refereed as soap. The RCOO− anions in water impulsively shape stable, spherical
aggregates known as micelles that dole out the useful detergent property of soaps. The micelle diameter is more
or less twice the length of the unmitigated FA. Concentrated preparations of micelles-water dispersions display
immense cleansing power. Usually look like pure water, these dispersions are quite stable. Foams and bubbles
on the exterior surface of soap dispersions results due to spontaneous adsorption of RCOO− ions at the edge
between the air and aqueous dispersion with the mechanically stretched and vigorously stabilized effect between
Chemical properties
Chemically, the acidic carboxyl group (COOH) of the fatty acid is the most reactive portion. It reacts with
alcohols (R′OH) to yield esters (RCOOR′) releasing water molecule. In complex lipids, the ester (principal
covalent) bond link FA moieties to other groups. Ether (R′−O−R, second chemical) bond also links FAs and are
In fatty acids, the hydrocarbon part is relatively defiant to chemical assail except C=C and different molecules
respond with similar double bond. For instance, when platinum is present as a catalyst, hydrogen append to the
double bond to bestow a SFA. Halogens (Iodine, Chlorine, Bromine) and their derivatives (hydroiodic acid) also
retort with the double bond to structure the SFAs, but herein the halogen (one or two atoms) swap the hydrogen
atoms (one or two) usually endowed in the saturated acyl chain. C=C can also respond with oxygen in either
enzymatically catalyzed oxidation reactions or non-enzymatic processes. The procedure engenders a range of
products, several of which add to the stale smell in vegetable products and spoiled meat. Universally, it is known
Properties of lipids explain its consistency, solubility, hydrolysis, saponification, emulsification and
rancidity.
Based on the chemical and physical properties, lipids undergo many reactions which are described below:
Alike other carboxylic acids, fatty acids demonstrate some of the key reactions i.e. they undergo acid-base
Acidity
The pKa of FAs do not illustrate a vast disparity in their acidities. For instance, nonanoic acid has a pK a of 4.96,
to some extent weaker than acetic acid (pKa 4.76). In water, the solubility of the FAs decreases very rapidly due
to increase in the chain length, so the longer chain fatty acids have least effect on aqueous solution pH. On the
other hand water insoluble FAs liquefy in warm ethanol can be titrated by NaOH solution using phenolphthalein
as an indicator with a pink endpoint. This testing is utilized to ascertain the FFA content of fats i.e. the
Hydrogenation is the phenomenon extensively accomplished for unsaturated fatty acids to give SFAs which are
not as much of decumbent to rancidification. Since the UFAs have inferior melting than the SFAs, the procedure
is called hardening. This knowledge and technology is employed to make margarine from vegetable oils. During
partial hydrogenation, the isomerization of UFAs from cis to trans configuration takes place. Use of high
temperature and high pressure (forcing hydrogenation) form fatty alcohols from fatty acids. However, fatty acid
esters convert to fatty alcohols more simply. Certain UFAs are cleaved in molten alkali in the Varrentrapp
metals and requires oxygen (air). Oils of vegetable origin defy this practice as they restrain antioxidants
(tocopherol). Oils and fats and oils are repeatedly are treated with citric acid (a chelating agents) to eliminate the
metal catalysts.
Fats & Oils: Sunflower, Peanut, Palm, Lard, Herring oil, Human fat, Coconut oil, Corn, Butter, Animal fats.