AARTI JAISWAL Reference: VID: 11196397318

Sample Collected At: Registered On:

CARDINAL GRACIAS MEMORIAL HOSP
21/08/2019 03:52 PM
TRUST 12
PID NO: P11190492409 BANGLI , SANDOR POST OFFICE VASAI Collected On:
Age: 27 Year(s) Sex: Female THANE 21/08/2019
Zone: W-26A(12)401201 Reported On:
22/08/2019 02:31 PM

Investigation Observed Value Unit Biological Reference Interval

Immature Platelet fraction

(EDTA Whole Blood)
Immature Platelet fraction 50.9 % 0.70-4.30
Platelet count 84 10^3 / µl 140-440
Medical Remarks: Platelet reduced on smear.
Method:
• Automated CBC counter based on Flow Cytometry principle
• IPF have a greater RNA content, are measured by automated hematology analyzers equipped with a reticulocyte detection
channel, and are reported as percentage of the total platelet count (%-IPF).
Clinical Background:

• Circulating immature platelets, also known as the immature platelet fraction (IPF), is the term that defines much larger
platelets that have been recently released from the bone marrow, presence of which show the thrombopoietic activity of the
marrow.
CLINICAL UTILITY:

It can help to distinguish between causes of thrombocytopenia due to

• Increased consumption/destruction (ITP) - High IPF

• Decreased production (marrow failure) – Low IPF
• It predicts the timing of platelet recovery (in Dengue, Malaria, after transplantation or chemotherapy)
• It can save platelet transfusions
◦ help to save a precious resource
◦ help to avoid potential infection risk
• IPF estimation can be used as guide for decisions concerning platelet transfusions & recovery of platelets in patients with
Dengue, Malaria, etc.
• The IPF% can predict the timing of platelet recovery. The platelet recovery time is 1-2 days of IPF increase.
• It was found that when the IPF starts going up (rising trend), 93.75% of the patients showed recovery within 24–48 h of the
rise. After a certain point when the IPF has peaked, then the platelets start coming up and IPF starts falling. This fall in the
IPF is a strong predictor of an impending rise in platelet count.
• As on most clinical scenarios follow-up IPF data for patients is not available, it has been shown in various studies that use of
a single time point IPF with cut-off value of 10% and above can be considered a good predictor for platelet recovery.
References:
• Assessment of an immature platelet fraction (IPF) in peripheral thrombocytopenia, Carol Briggs, Stefan Kunka,Dan Hart,
Shinichiro Oguni and Samuel J. Machin, British Journal of Haematology, 126, 93–99
• Dadu, T., Sehgal, K., Joshi, M, Khodaiji, S. Evaluation of the immature platelet fraction as an indicator of platelet recovery in
dengue patients. International Journal of Laboratory Hematology, 2013; 2013; 49(7)10.

Page 1 of 3 Dr. Rina Shah

M.D (Pathology)
Consulting Pathologist
 AARTI JAISWAL Reference: VID: 11196397318
Sample Collected At: Registered On:
CARDINAL GRACIAS MEMORIAL HOSP
21/08/2019 03:52 PM
TRUST 12
PID NO: P11190492409 BANGLI , SANDOR POST OFFICE VASAI Collected On:
Age: 27 Year(s) Sex: Female THANE 21/08/2019
Zone: W-26A(12)401201 Reported On:
22/08/2019 02:31 PM

Investigation Observed Value Biological Reference Interval

Anti Nuclear Antibody by IFA

(Serum,Immunofluorescence)
Result Negative Negative
Pattern - -
Grade - -
Estimated Titre - -

Interpretation Guidelines (Sample screening Dilution - 1:100):

Negative : No Immunofluorescence
+ : Weak Positive (1:100)
++ : Moderate Positive (1:320)
+++ : Strong Positive (1:1000)
++++ : Very strong Positive (1:3200)

Test Description:

Antinuclear antibodies (ANAs) are unusual antibodies, detectable in the blood, that have the capability of binding to certain
structures within the nucleus of the cells. ANAs indicate the possible presence of autoimmunity & provide, therefore, an indication of
autoimmune illness. Fluorescence tech. are frequently used to actually detect the antibodies in the cells, thus ANA testing is
sometimes referred to as fluorescent antinuclear antibody test (FANA). The ANA test is a sensitive screening test used to detect
autoimmune diseases.

Technique:

Indirect Immunofluorescence - Automated IF Processor (AP 16 IF Plus)

The BIOCHIP Slide is a combination of Hep-20-10 cells and primate liver and has the following advantages.

• It is a global standard tech. with a natural antigen spectrum capable of detecting more than 30 diagnostically relevant auto
antibodies.

• Hep 20-10 cell lines contain 40% mitotic cells, facilitating easier identification of rare patterns.

• If the test is negative, detectable level of auto antibodies is ruled out. In case of a positive result, autoantibodies against any
one or in some cases simultaneously against more than one antigens may be present and further monospecific tests or
panel of profiles can be used to determine the specific autoantibodies present.
• NOTE- All weak positive (+) results may be repeated after 6 - 8 weeks.

Associated Tests: Monospecific ELISA to define single antigens, ANA Immunoblot assay.

Abbreviations: SLE: Systemic Lupus Erythematosus, SCL: Scleroderma, MCTD: Mixed Connective Tissue Disease; CFS: Chronic
Fatigue Syndrome; AIH: Autoimmune Hepatitis, PBC: Primary Bilary Cirrhosis, PM:Polymyositis, DM:Dermatomyositis, SS: Systemic
sclerosis, RA:Rheumatoid Arthritis.

Please view next page for co-relation table including various single antigens with their Immunoflourescence patterns and
clinical associations.

Page 2 of 3 Dr. Rina Shah

M.D (Pathology)
Consulting Pathologist
 AARTI JAISWAL Reference: VID: 11196397318
Sample Collected At: Registered On:
CARDINAL GRACIAS MEMORIAL HOSP
21/08/2019 03:52 PM
TRUST 12
PID NO: P11190492409 BANGLI , SANDOR POST OFFICE VASAI Collected On:
Age: 27 Year(s) Sex: Female THANE 21/08/2019
Zone: W-26A(12)401201 Reported On:
22/08/2019 02:31 PM

Investigation Observed Value Unit Biological Reference Interval

Location Pattern Target Antigen Clinical Association

Nucleus Homogeneous Double strand DNA SLE

Histones Drug Induced Lupus, SLE ,
Nucleosome, RNA,Single RA
Strand DNA SLE, MCTD,RA, PM, DM, SS
Speckled Sm SLE
U1-snRNP MCTD,SLE,RA, sharp
SSA/Ro syndrome
SSB/La Sjogren`s syndromes
Ku (SS)/SLE/Neonatal Lupus
Cyclin1(PCNA) PM/DM/SLE/SS
Mitosin/Cyclin II SLE/Overlap Syndromes
DM
Dense Fine Speckled(DFS) Lens epithelium-derived Healthy individuals, Various
growth factor (LEDGF), DNA Inflammatory conditions like
binding transcription atopic dermatitis, interstitial
coactivator p75.(DFS-70) cystitis, Asthma.
Centomeres Proteins of Kinetochores CREST syndrome, PSS
limited form
Nuclear Dots Sp-100 , NDP53 PBC,Rheumatic Disease
Nuclear Membrane Lamins, gp210, p62 CFS,Collagenoses,PBC,AIH
Nucleolus Nucleolar homogeneous PM-Scl PM, DM, PSS(Diffuse)
Scl-70 PSS(Diffuse)
Nucleolar speckled RNA-Polymerase I / NOR-90 Progressive Systemic
Sclerosis(Diffuse)
Nucleolar Pattern Fibrillarin Progressive Systemic
Sclerosis(Diffuse)
Cytoplasm Cytoplasmic speckled Mitochondrial PBC,
Lysosomal Unknown
Golgi Complex SS/SLE/RA
Ribosome P SLE
Jo -1 Polymyositis (PM),
SRP, PL12, TIF1-Gamma PM/ DM, Myositis
Cytoplasmic filament F-Actin AIH
Vimentin Unknown
Tropomyosin Unknown
Cytoplasmic Rings & rods HCV Infection- on therapy
Cell Cycle (mitotic cells) Centriole -- Unknown
Mid-Body -- Unknown
Spindle Fibres -- Rheumatic Disease

-- End of Report --

Page 3 of 3 Dr. Rina Shah

M.D (Pathology)
Consulting Pathologist