Total Phenolic compound determination assay

Concentration
Absorbance (nm)
(µg/mL)
500 1.98
250 1.07
125 0.52
62.5 0.27
31.25 0.16
15.63 0.08

TPC determination Assay

2.5
y = 0.0039x + 0.034
2 R² = 0.9987
Absorbance (nm)

1.5
Absorbance (nm)

1 Linear (Absorbance (nm))

0.5

0
0 200 400 600
Concentration (µg/mL)

Sample Weight Absorbance GAE GAE TPC as Mean

solution of dry (nm) Conc. Conc. GAE (mg/gm)
(µg/mL) extract C C A=(c×v)/m
per mL (µg/mL) (mg/mL) (mg/gm)
m(mg)
1000 0.001 1.770 445.13 0.445 445.13
1000 0.001 1.767 444.36 0.444 444.36 442.57
1000 0.001 1.743 438.21 0.438 438.21
Total Flavonoid content determination assay

Concentration
Absorbance (nm)
(µg/mL)
500 1.846
250 0.727
125 0.564

TFC determination Assay

2 1.846
1.8
y = 0.0036x + 0.0045
1.6
R² = 0.9539
1.4
Absorbance (nm)

1.2 Absorbance (nm)

1 Linear (Absorbance (nm))
0.727
0.8
0.564
0.6
0.4
0.2
0
0 100 200 300 400 500 600
Concentration (µg/mL)

Sample Weight of Absorbance GAE GAE TPC as Mean

solution dry extract (nm) Conc. Conc. GAE (mg/gm)
(µg/mL) per mL C C A=(c×v)/m
m(mg) (µg/mL) (mg/mL) (mg/gm)
1000 0.001 0.356 97.64 0.098 97.64
1000 0.001 0.366 100.42 0.100 100.42 99.68
1000 0.001 0.368 100.97 0.101 100.97
Thrombolytic assay

Empty Weight Weight Weight Final Percentage Mean

weight of clot of clot, of clot weight (%) of clot (%)
of tube, with C=B-A with tube of lysis lysis
A tube, (gm) after clot,
(gm) B lysis, E=B-D
(gm) D (gm)
(gm)
0.85 1.23 0.38 1.15 0.08 21.05
0.81 1.16 0.35 1.09 0.07 20
0.84 1.18 0.34 1.11 0.09 20.59 20.42
0.84 1.20 0.36 1.14 0.06 16.66
0.8 1.22 0.42 1.12 0.1 23.81

Group % 0f clot lysis

Streptokinase 48.72
Extract 20.42
Distilled water 2.63

% 0f clot lysis
60
48.72
50
% of clot lysis

40

30 Streptokinase
20.42
Extract
20
Distilled water
10
2.63
0
Streptokinase Extract Distilled water
Group
Anthelmintic Assay

Concentration Paralysis time

Test Sample (mg/mL) (min) Death time (min)
1 4.5 8.55
Standard
0.8 7.4 12.26
(Levamisole)
0.5 15.15 45.3
10 14.25 27.3
MeOH extract 5 19.1 36.25
2.5 33.34 54.3

60
Concentration (mg/mL) Paralysis time (min) Death time (min) 54.3

50
45.3

40 36.25
33.34
Time (min)

30 27.3

19.1
20
15.15 14.25
12.26
10
10 8.55 7.4
4.5 5
2.5
1 0.8 0.5
0
Standard MeOH extract
(Levamisole)
DPPH Antioxidant Assay
Table: 01

Control: 1.265

Concentration % inhibition of Ascorbic

Absorbance (nm)
(µg/mL) acid IC50 (µg/mL)
15.63 0.775 38.73517787
31.25 0.694 45.13833992
62.5 0.473 62.60869565
7.84
125 0.378 70.11857708
250 0.045 96.44268775
500 0.027 97.86561265

% inhibition of Ascorbic acid

120

100

80
% inhibition of Ascorbic
% inhibition

acid
60 y = 0.1183x + 49.072
Linear (% inhibition of
R² = 0.7726
40 Ascorbic acid)

20

0
0 200 400 600

Concentration (µg/mL)
Table: 02

Control: 1.024

Concentration
Absorbance (nm) % inhibition of Extract IC50 (µg/mL)
(µg/mL)
15.63 0.595 41.89453125
31.25 0.577 43.65234375
62.5 0.461 54.98046875
31.71
125 0.293 71.38671875
250 0.269 73.73046875
500 0.087 91.50390625

% inhibition of Extract
120

100

80
% inhibition

60 % inhibition of Extract
y = 0.0972x + 46.918
R² = 0.8615
40 Linear (% inhibition of
Extract)
20

0
0 200 400 600
Concentration (µg/mL)
Table:03
Concentration % inhibition of Ascorbic
(µg/mL) acid % inhibition of extract
15.63 38.73517787 41.89453125
31.25 45.833992 43.65234375
62.5 62.60869565 54.98046875
125 70.11857708 71.38671875
250 96.44268775 73.73046875
500 97.86561265 91.50390625

% inhibition of DPPH
120
y = 0.1178x + 49.276
R² = 0.7736
100

80
% inhibition

y = 0.0972x + 46.918
R² = 0.8615
60

40

% inhibition of Ascorbic acid

% inhibition of extract
20 Linear (% inhibition of Ascorbic acid)
Linear (% inhibition of extract)

0
0 100 200 300 400 500 600

Concentration (µg/mL)
Reducing Power Assay

Concentration Absorbance of Ascorbic acid Absorbance of Extract

(µg/mL) (nm) (nm)
15.63 0.376 0.325
31.25 0.874 0.398
62.5 1.472 0.409
125 2.1 0.424
250 2.364 0.446
500 2.587 0.495

Reducing power activity

3.5

3
y = 0.0039x + 0.99
2.5 R² = 0.6774
Absorbance (nm)

Absorbance of Ascorbic acid

2 (nm)
Absorbance of Extract (nm)

1.5 Linear (Absorbance of

Ascorbic acid (nm))
Linear (Absorbance of Extract
1
y = 0.0003x + 0.3728 (nm))
R² = 0.7572
0.5

0
0 100 200 300 400 500 600
Concentration (µg/mL)