LAAN-A-LC-E100

Application Data Sheet

High Performance Liquid Chromatography No. 31

High-Speed Analysis of Aldehydes and Ketones

This is an example of simultaneous analysis of a mixture of 15 aldehydes and ketones.

mAU

225 Peaks
1 1. Formaldehyde-2,4-DNPH 8. Benzaldehyde-2,4-DNPH
200 2. Acetaldehyde-2,4-DNPH 9. Isovaleraldehyde-2,4-DNPH
3. Acetone-2,4-DNPH 10. Valeraldehyde-2,4-DNPH
175 4. Acrolein-2,4-DNPH 11. o -Tolualdehyde-2,4-DNPH
5. Propionaldehyde-2,4-DNPH 12. m -Tolualdehyde-2,4-DNPH
150 6. Crotonaldehyde-2,4-DNPH + -Tolualdehyde-2,4-DNPH
7. Butyraldehyde-2,4-DNPH 13. Hexaldehyde-2,4-DNPH
125 14. 2,5-Dimethylbenzaldehyde-2,4-DNPH
2
100
4
3
75
5 12
9
50
6 8 10
7
11
13
25 14

0.0 1.0 2.0 3.0 4.0 5.0 6.0 7.0 8.0 9.0 min

Analysis of Standard Sample Mixture of Aldehydes and Ketones

[Sample Preparation]
T011/IP-6A Aldehyde/Ketone-DNPH Mix (SUPELCO Analytical, concentration of 15 g/mL/component) was
diluted with 2 times that amount of distilled water.

Analytical Conditions
Instrument : Prominence UFLC system
Column : Shim-pack XR-ODS (75 mm x 3.0 mm i.d.)
Mobile Phase : A) Water / Tetrahydrofuran / Methanol =7 / 2 / 1 (v/v/v)
B) Water / Acetonitrile = 4 / 6 (v/v)
B.conc; 45% to 60% (0 to 4.1 min), 60 to 100% (4.1 to 5.4 min),
100% (5.4 to 9.6 min) , 45% (9.7 to 13 min)
Flow Rate : 1.0 mL/min
Column Temperature : 50 C
Detection : Absorbance at 360 nm with Semi-micro flow cell
Sample Volume : 4 µL

SHIMADZU CORPORATION. International Marketing Division

3, Kanda-Nishikicho 1-chome, Chiyoda-ku, Tokyo 101-8448, Japan Phone. 81(3)3219-5641 Fax. 81(3)3219-5710
URL http://www.shimadzu.com Issued in October, 2007