NAME: PARDILLO, MELODY JANE B.

SECTION: BMLS 10-2C

Bacteriological diagnostics of intestinal pathogens with real-time PCR -

An evaluation of two molecular biology applications
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Publication Bachelor thesis

Title Bacteriological diagnostics of intestinal pathogens with real-
time PCR - An evaluation of two molecular biology applications
Author Johansson, Christian
Date 2018
English abstract
Intestinal infections possesses a prominent problem in medical health care,
primarily in the form of the increasing number of clinical patient samples
caused by these infectious intestinal diseases. Hence, could a introduction of
molecular biology methods may be vital as a complimenting screening
method, with the intention of shortening existing analytic time without a
reduction or limitation of analytical sensitivity and specificity. The study´s
design involves an evaluation of the two commercial real-time PCR
applications: Amplidiag® Bacterial GE Assay and BD-MaxTM Enteric
Bacterial Panel Assay with the use of clinical retrospective patient samples (n
= 242). Both of these methods detected a total of eight bacterial pathogens
each, five of which were measured against the reference method. The
methods received high specificity of >98% for Salmonella, Campylobacter,
Shigella, EHEC, and Yersinia. And showed a sensitivity of >90% for
Campylobacter, Yersinia and EHEC, while Salmonella and Shigella obtained
a dissentient sensitivity of <90%. No statistically significant difference could be
detected for the Amplidiag® (p-value = 1.00) or for the BD-Max (p-value =
0.655) when compared to the available culture diagnostics. This study shows
a strong argument to propose multiplex real-time PCR panels as a foundation
for a reformed diagnostic method of intestinal pathogens. Based on a
prominent improvement of factors such as; the time aspect and the
performance of the method.