Pulp dentin complex

regeneration
final assignment for tissue engineering course
 re·gen·er·a·tion  noun
The act or process of regenerating or the state of being regenerated.
Spiritual or moral revival or rebirth.

Regrowth of lost or
Biology 

destroyed parts or organs.

The American Heritage® Dictionary of the English Language, 4th edition Copyright © 2010 by Houghton Mifflin
Harcourt Publishing Company. Published by Houghton Mifflin Harcourt Publishing Company.
Pulp dentin complex:

why complex ???

Dentin and pulp are
embryologically, histologically, and
functionally the same tissue and
therefore are considered as a
complex
Odontoblast cell layer
One of the
most
important
functions
of this
complex is
the
reparative,
formative
function ….
Previous trials to repair or regenerate dentin :

“Regenerative dental procedures have a

long history, originating around 1952,
when Dr. B. W. Hermann reported on
the application of Ca(OH)2 in a case
report of vital pulp amputation”

vital pulp therapy.

apxification.
So it is evolution not a revolution to use the
next step …….tissue engineering.

Regenerative endodontics ….

“Regenerative endodontic procedures can be

defined as biologically based procedures
designed to replace damaged
structures, including dentin and root
structures, as well as cells of the pulp-
dentin complex”
Regenerative Endodontics: A Review of Current Status and a Call for Action
Peter E. Murray, BSc(Hons), PhD, Franklin Garcia-Godoy, DDS, MS, and Kenneth M. Hargreaves,
DDS, PhD
Dental pulp stem cells:
DPSCs were found to differentiate into odontoblast like Cells.
DPSCs were also found to differentiate into adipocytes or
neural-like cells
These cells can be induced to undergo uniform differentiation
into smooth and skeletal muscle cells, neurons, cartilage and
bone cells under chemically defined culture conditions.

Ectomesenchymal stem cells of human exfoliated

deciduous teeth (SHEDs):
They induced bone formation and produced dentin
under in vivo conditions; and they were able to survive and
migrate in murine brain after transplantation into
immunocompromised
animals
Regeneration of dentine/pulp-like tissue using a
dental pulp stem cell/poly(lactic-co-glycolic) acid
scaffold construct in New Zealand white rabbits

Rania M. El-Backly, BDS, MS; Ahmed G. Massoud, BDS, MS, PhD; Azza
M. El-Badry, BDS, MS, PhD;
Raef A. Sherif, BDS, MS, PhD; and Mona K. Marei, BDS, MS, PhD
Dental pulp stem cells were isolated from rabbit teeth
and seeded onto scaffolds prepared from 50/50
poly(lactic-co-glycolic acid) polymers using two
different porogen particle sizes.

These cell/scaffold constructs were then transplanted

subcutaneously in the rabbits.

Histological evaluation of transplanted samples

revealed the formation of osteodentine-like
structures as well as tubular bilayered structures of
vertically aligned parallel tubules resembling
tubular-like dentine.
“Finally, results from the present work managed to
display the capacity of using a tissue engineering
concept to regenerate a variety of dentine-like and
pulp-like tissues. The regenerated tissues appeared to
be quite similar to well-known repair tissues seen in in
vivo in situ situations. This indicates that tissues
regenerated by tissue engineering can closely simulate
those that are formed by naturally occurring events”
Thank you