ENVIRONMENTAL ENGINEERING LAB

Determination of dissolved oxygen in water

‫براءه بسام ابوراس‬

31615019092

Eng. Aysha As-hab

Monday 2-5
  Introduction
The level of dissolved oxygen in water is one of the most important parameters in determining its
quality, because it indirectly indicates whether there is some kind of pollution.
The measurement of the DO is also important to determine whether a water system is
predominantly aerobic or anaerobic, predict the survival of aquatic organisms, and predict
whether aerobic biological processes can take place for transforming the biodegradable organic
contaminants discharged in water.
The DO depends on water temperature, dissolved salts, atmospheric pressure (and therefore of
altitude), the presence of reducing compounds, suspended matter, and living species. The aquatic
flora and fauna can contribute to the consumption or to the production of oxygen in water. For
the aquatic flora, the photosynthesis process is responsible for the generation of oxygen in the
presence of light; this explains the DO fluctuations during the day/night cycles and in the
different seasons.

 Data

 Calculations
DO = Volume of S2O3-2 * N * 1000 / V sample

= 7.9 * 0.025 * 1000 / 200

= 0.9875 mg/L
  Winkler Method
The Winkler Method is a technique used to measure dissolved oxygen in freshwater systems.
Dissolved oxygen is used as an indicator of the health of a water body, where higher dissolved
oxygen concentrations are correlated with high productivity and little pollution. This test is
performed on-site, as delays between sample collection and testing may result in an alteration in
oxygen content.

 Conclusion
In this experiment we measured the
amount of dissolved oxygen in water in
gm/L by Winkler’s method.

The value I think it’s not reasonable,

maybe too small; or the known numbers
are not correct. However there shouldn’t
be any air gap or bubbles in the reaction
bottle to get the right values.