EXPERIMENT # 6

DISSOLVED OXYGEN LEVELS

IN NATURAL WATERS

NAME: AGUSTIN, SETH JARL G. CM011L/A3

PROGRAM/YEAR: CE - 1 DOP: APRIL 9, 2020

GROUP N0. 3 DOS: APRIL 29, 2020

PRELIM DATA SHEET: /50

COVER: /10

OBJECTIVE: /10

REPORT SHEET: /20

CONCLUSION: /10

ELIZABETH ESPIRITU TOTAL: /100

PROFFESOR
 EXPERIMENT # 6
DISSOLVED OXYGEN LEVELS IN
NATURAL WATERS

OBJECTIVES:

1. TO DEVELOP A PROPER TECHNIQUE FOR OBTAINING

A NATURAL WATER SAMPLE.DDDDDDDDDDDDDDDD
DSSDSDSDSDSDSDSDSDSDSDSDSSDDSSDSDSDSDSD
2. TO DETERMINE THE DISSOLVED OXYGEN
CONCENTRATION OF A NATURAL WATER SAMPLE.
DDDD
3. TO LEARN THE CHEMICAL REACTIONS INVOLVED IN
FIXING AND ANALYZING A WATER SAMPLE FOR
DISSOLVED OXYGEN USING THE WINKLER METHOD.
 PRELABORATORY ASSIGNMENT:

1. For a natural water sample, what range of dissolved oxygen concentrations may
you expect? Explain your reasoning.
Dissolved oxygen can range from zero up to saturation for the temperature of
the water. The greater the contact with air, the more turbulence in water then
the greater dissolved oxygen.

2. Experimental Procedure, Parts A.4,5.

a. Ambient temperature changes?
Solubility of oxygen decrease as temperature increase, therefore the warmer
the surface of water, the less it requires dissolved oxygen.

b. Atmospheric pressure changes?

Water at lower altitudes can hold more dissolved oxygen, therefore, the
higher atmospheric pressure, and the lower dissolved oxygen.

c. The volume of the flask collecting the water sample?

There will be more air present in a bigger flask, therefore, the bigger the
volume of the flask, the more dissolved oxygen.

d. The amount of organic matter in the water sample?

The more amount of organic matter, the lower the dissolved oxygen.

e. The depth of the body of water (e.g., lake, river,or ocean)?

The deeper we go in a body of water, the less oxygen will be present,
therefore, the deeper the body of water the less oxygen dissolved.
3. Experimental Procedure, Parts A.1. A 100-mL volume of a primary standard
0.0110 M KIO₃ solution is prepared. A 25.0-mL aliquot of this solution is used to
standerize a prepared Na₂S₂O₃ solution. A 15.6-mL volume of the Na₂S₂O₃ solution
titrated the KIO₃ solution to the starch endpoint. What is the molar concentration of
the Na₂S₂O₃ solution?

IO₃⁻(aq) + 8I⁻(aq) + 6H⁺(aq) ⇒ 3I₃⁻(aq) + 3H₂O(l)

I₃⁻(aq) + 2S₂O₃²⁻(aq) ⇒ 3I₃⁻(aq) + S₄O₆²⁻(aq)

4. Experimental Procedure, Part A.2. What is the procedure for preparing 250 mL of
0.0210 M of Na₂S₂O₃ for this experiment from a 100-mL volume of standard 0.106 M
Na₂S₂O₃?

Dilute the 43.5 mL of 0.106 M of Na₂S₂O₃

5. a. An analyses of a water sample for dissolved oxygen levels provided the data
in the following table. Complete the analyses for Trial 1 (see Report Sheet.) of the
water sample. Record the calculated values using the correct number of significant
figures.

A. Standard 0.025 M Na₂S₂O₃

1. Sample Volume (mL) 200 mL

2. Buret reading, initial (mL) 3.85 mL
3. Buret reading, final (mL) 18.25 mL
4. Volume Na₂S₂O₃ dispensed (mL) 14.40 mL
5. Average Molar Concentration of Na₂S₂O₃ (mol/L) 0.0213 mol/L
6. Moles of Na₂S₂O₃ dispensed (mol) 3.07 x 10⁻⁴ mol
7. Moles of I₃⁻ reduced by S₂O₃²⁻(mol) 1.53 x 10⁻⁷ mol
8. Moles of O₂ (mol) 7.67 x 10⁻⁵ mol
9. Mass of O₂ (mg) 2.40 mg
10. Dissolved Oxygen ppm O₂ (mg/L) 6.965 mg/L
SOLUTIONS:
5. b. For Trial 2 and 3, the dissolved oxygen levels were 10.9 ppm and 11.1 ppm
respectively.

a. What is the average dissolved oxygen level in the water sample?

b. What are the standard deviation and the relative standard deviation (%RSD) of
the dissolved oxygen level in the water sample?
 CONCLUSION & RECOMMENDATIONS:

A water's quality is not based how much filtration or boiling

is done to is, but also the existing dissolved oxygen within
it. Having dissolved oxygen in water is one the important
indicators of its quality. Much more can be observed from
this dissolved oxygen, as it is also essential for marine
species that solely exist from the oxygen contained in the
water. Like us breathing oxygen from atmosphere, these
aquatic anime breathe oxygen from the water. In some
cases, the red tide occurring in the sea depletes oxygen
from the water causing the deaths of these animals that
only rely on the dissolved oxygen in the water.

The results from the pre-laboratory assignment showed that

the dissolved oxygen concentration is 6.965 mg/L, with
average dissolved oxygen level computed being 12.3 ppm
O₂, standard deviation 11.4 and the relative standard
deviation 7.13%.

I have learned in this experiment that just as much oxygen

in the atmosphere is important for us, it is also vital for the
marine animals to survive. The colder the water, the more
oxygen it stores, and the warmer it is, the less oxygen it
holds.