LABORATORY EXPERIMENT

NO. 6

DISSOLVED
OXYGEN LEVELS
IN NATURAL
WATERS
Arthur Lechoncito Sir Adonis Adornado
 LAB QUESTIONS
1. Part B. The water chemist waits until returning to the laboratory to fix the water sample
for the dissolved oxygen analysis. Will the reported dissolved oxygen concentration be
reported as too high, too low, or remain unchanged? Explain.
o It is unchanged because the water chemist didn’t tamper and only left the
water sample.
2. Part B. 3 A solution of MnSo4 is added to fix the dissolved oxygen in the collected
sample.
a. What is the meaning of the expression, “fix the dissolved oxygen,” and why is it so
important for the analysis of dissolved oxygen in a water sample?
o The expression refers to the procedure that’s needed to determine the
concentration of the dissolved oxygen in the sample. It is very useful when
determining the magnitude of biological activity in the water sample.
b. Only an approximate volume (-1mL) of MnSo4 is required for fixing the dissolved
oxygen in the sample. Explain why an exact volume is not critical.
o An exact volume is not critical because adding 1mL isn’t that required. Having
approximation won’t really have an affect to the precipitate that will come.
3. Part B.4 No precipitate forms! Assuming the reagents were properly prepared and
dispensed into the sample, what might be predicted about its dissolved oxygen
concentration? Explain.
o The precipitate’s value is proportional to the concentration of the dissolve
oxygen, and if the reagent is dispensed properly and that there are no
precipitates then there would be zero dissolved oxygen concentration.
4. Part B.5. A water chemist measured and recorded the air temperature at 27oC when he
should have measured the water temperature, which was only 21OC. As a result of this
error, will the dissolved oxygen concentration be reported as being higher or lower than
it should be? Explain
o If the water chemist measured and recorded the air temp at 27C when he
should have measured the water temp which is only 21C then the result will be
too high because the oxygen concentration in the water is inversely
proportional to the temperature.
5. Part C.3. The color of the analyte did not fade to form the light yellow-brown color but
remained intense even after the addition of a full burette of the S2O32- titrant, even
though a precipitate formed in Part B.4. What can be stated about the dissolved oxygen
concentration of the sample? Explain.
o If the color of the analyte did not fade to form the light yellow-brown color but
remained intense then it means that they failed precipitating it. It must turn
light yellow-brown because the triiodide ion is indeterminable. Titration is a
must when determining the amount of triiodide ions. This can also help
determine and calculate the dissolved oxygen concentration which can help us
have an accurate determination of the concentration of dissolved oxygen.
6. Assuming a dissolved oxygen concentration of 7.0 ppm (mg/L) in a 200-mL water
sample.
𝐦𝐦𝐦𝐦 𝟏𝟏𝟏𝟏 𝟏𝟏 𝐦𝐦𝐦𝐦𝐦𝐦 𝐎𝐎𝟐𝟐
𝟕𝟕 ∗ 𝟎𝟎. 𝟐𝟐𝟐𝟐 ∗ ∗ = 𝟒𝟒. 𝟑𝟑𝟑𝟑𝟑𝟑 𝐱𝐱 𝟏𝟏𝟏𝟏−𝟓𝟓 𝐦𝐦𝐦𝐦𝐦𝐦 𝐎𝐎𝟐𝟐
𝐋𝐋 𝟏𝟏𝟏𝟏𝟏𝟏𝟏𝟏𝟏𝟏𝟏𝟏 𝟑𝟑𝟑𝟑 𝐠𝐠

A. How many moles of Mn(OH)3 will be produced with the addition of the
MnSO4 solution?
𝟒𝟒 𝐦𝐦𝐦𝐦𝐦𝐦 𝐌𝐌𝐌𝐌(𝐎𝐎𝐎𝐎)𝟐𝟐
o 𝟒𝟒. 𝟑𝟑𝟑𝟑𝟑𝟑 𝐱𝐱 𝟏𝟏𝟏𝟏−𝟓𝟓 𝐦𝐦𝐦𝐦𝐦𝐦 𝐎𝐎𝟐𝟐 ∗ = 𝟏𝟏. 𝟕𝟕𝟕𝟕 𝐱𝐱 𝟏𝟏𝟏𝟏−𝟒𝟒 𝐦𝐦𝐦𝐦𝐦𝐦 𝐌𝐌𝐌𝐌(𝐎𝐎𝐎𝐎)𝟐𝟐
𝟏𝟏 𝐦𝐦𝐦𝐦𝐦𝐦 𝐎𝐎𝟐𝟐
B. How many moles of I3- will be produced when the KI-NaN3 solution is added
to the above solution?
𝟏𝟏 𝐦𝐦𝐦𝐦𝐦𝐦 𝐈𝐈𝟑𝟑
o 𝟏𝟏. 𝟕𝟕𝟕𝟕 𝐱𝐱 𝟏𝟏𝟏𝟏−𝟒𝟒 𝐦𝐦𝐦𝐦𝐦𝐦 𝐌𝐌𝐌𝐌(𝐎𝐎𝐎𝐎)𝟐𝟐 ∗ = 𝟖𝟖. 𝟕𝟕𝟕𝟕 𝐱𝐱 𝟏𝟏𝟏𝟏−𝟓𝟓 𝐦𝐦𝐦𝐦𝐦𝐦 𝐈𝐈𝟑𝟑
𝟐𝟐 𝐦𝐦𝐦𝐦𝐦𝐦 𝐌𝐌𝐌𝐌(𝐎𝐎𝐎𝐎)𝟐𝟐
C. How many moles of S2O32- will be needed to react with the I3- that is
generated?
𝟐𝟐 𝐦𝐦𝐦𝐦𝐦𝐦 𝐒𝐒𝟐𝟐 𝐎𝐎𝟑𝟑 𝟐𝟐−
o 𝟖𝟖. 𝟕𝟕𝟕𝟕 𝐱𝐱 𝟏𝟏𝟏𝟏−𝟓𝟓 𝐦𝐦𝐦𝐦𝐦𝐦 𝐈𝐈𝟑𝟑 ∗ = 𝟏𝟏. 𝟕𝟕𝟕𝟕 𝐱𝐱 𝟏𝟏𝟏𝟏−𝟒𝟒 𝐦𝐦𝐦𝐦𝐦𝐦 𝐒𝐒𝟐𝟐 𝐎𝐎𝟑𝟑 𝟐𝟐−
𝟏𝟏 𝐦𝐦𝐦𝐦𝐦𝐦 𝐈𝐈𝟑𝟑
D. and assuming the concentration of the S2O32- titrant to be 0.025 M, how
many milliliters of titrant will be predictably used for the analysis.
𝟏𝟏 𝐋𝐋 𝐒𝐒𝟐𝟐 𝐎𝐎𝟑𝟑 𝟐𝟐− 𝟏𝟏𝟏𝟏𝟏𝟏𝟏𝟏 𝐦𝐦𝐦𝐦
o 𝟏𝟏. 𝟕𝟕𝟕𝟕 𝐱𝐱 𝟏𝟏𝟏𝟏−𝟒𝟒 𝐦𝐦𝐦𝐦𝐦𝐦 𝐒𝐒𝟐𝟐 𝐎𝐎𝟑𝟑 𝟐𝟐− ∗ 𝟐𝟐− ∗ = 𝟕𝟕 𝐦𝐦𝐦𝐦 𝐨𝐨𝐨𝐨 𝐒𝐒𝟐𝟐 𝐎𝐎𝟑𝟑 𝟐𝟐−
𝟎𝟎.𝟎𝟎𝟎𝟎𝟎𝟎 𝐦𝐦𝐦𝐦𝐦𝐦𝐒𝐒𝟐𝟐 𝐎𝐎𝟑𝟑 𝟏𝟏 𝐋𝐋
7. A nonscientist brings a water sample to your laboratory and asks you to determine why
there was a fish kill in the nearby lake. Having recently finished this experiment, what
might you tell that person about the legitimacy of a test for dissolved oxygen? What
reasoning would you use to maintain the integrity of your laboratory?
o Marine life can adapt to slow changes. These changes can be a rise in
temperature or even a lowered dissolved oxygen level. But fish kill can occur
when the chemicals that dissolves oxygen are present. Fishes needs oxygen to
live, since an element that dissolves oxygen is present in a lake, oxygen are
insufficient to be breath by the fishes. Fish kill can also occur when oxygen and
elements that dissolve it is insufficient.

8. A. Fish kills are often found near the discharge point of water from cooling waters at
electrical generating power plants. Explain why this occurrence may occur.
o Fish kills are often found near the discharge point of water from cooling waters
at electrical generating power plants because a electric generating power plant
needs to boil water so that it could produce high-pressure steam that helps
turbines turn to produce electricity. To cool the steam, it uses a condenser which
uses water drawn in from a body of water the cooling water is then not reused,
instead it is dumped with a higher temperature back into the water body which
can harm the fish living in the body of water.
B. Fish kills are often found in streams following heavy rainfall in a watershed
dominated by farmland or denuded forestland. Explain why this occurrence may
occur.
o Material that sinks in the liquid is worn-away and moved mostly during heavy
rainfall events and the connected high stream flows, especially floods. material
that sinks in the liquid can become a problem because its removal from a ruling
position or legal statement under oath in streams and lakes can ruin the home
for water-based plants and animals especially fishes. The most important factor
for material that sinks in liquid transport is the amount of land cleared of green
plants. material that sinks in the liquid sources usually don't have enough
developed areas, but during land or construction, when little vegetative cover
exists, the exposed soil can be easily wore away during storms and deposited in
downstream water ways.
9. Explain how the dissolved oxygen concentrations may change starting at the
headwaters of a river and ending at the ocean. Account for the changes.
o Dissolved oxygen concentration at the headwater of a river is higher and can
tend go to lower as it go down to the oceans and the dissolved oxygen might be
more bothered to the end of the ocean compared to the headwater of a river
because there are bacteria which can consume oxygen and there are more
bacteria in a river compare to the ocean.
10. Salt (ocean) water generally has a lower dissolved oxygen concentration than
freshwater at a given temperature. Explain why this is generally observed.
o Salt (ocean) water has a lower dissolved oxygen concentration because is it more
exposed to the sun. Daylight is essential for oxygen to be broken down.
 DOCUMENTATION
Preparing the materials

Filling the 500mL bottle with water to the brim

Adding 2mL of MnSO4 to the bottle

Flipping the bottle for the reagents to form precipitates

Waiting for the precipitates to set

Dissolving the precipitates by adding 3mL of concentrated H2SO4

Dissolving the precipitates by adding 3mL of concentrated H2SO4

Aliquot with completely dissolved precipitates

Withdrawing 200mL aliquot

Filling the burette with Na2S2O3

Titrating the aliquot until very light yellow in color

Getting 1mL of starch solution

Adding 1mL starch solution to the aliquot

Titrating using Na2S2O¬3 until the aliquot becomes colorless

Colorless aliquot
 CONCLUSION
This experiment is all about dissolved oxygen levels in natural waters. The objective for this
experiment is to develop a proper technique for obtaining natural water sample, to determine
the dissolve oxygen concentration of a natural water sample and to learn the chemical
reactions involved in fixing and analyzing a water sample for dissolve oxygen using the Winkler
method. The Winkler method was developed by Lajos Winkler where it is the standard
experimental procedure for determining the dissolve oxygen concentration in water and for the
calibration of dissolve oxygen meters. To do this experiment first we filled up a bottle with
500ml tap water up to the brim and it doesn’t have any bubbles then we added 2 mL MnSO4
solution via pipette then we added 2 mL KI-NaN3 solution via pipette then we let the
precipitate settle at the bottom, then we added 3 mL H2SO4 to dissolve the precipitate and we
also flipped the botte several times, then we withdraw 200mL aliquot, then we titrated with
NaS2O3 until it turns very light yellow, then we added 1mL starch solution which will turn the
solution to dark blue, then we titrated it again until it turns colorless and finally we get the
volume of NaS2O3.
PRINTED BY: 1fa8ca33c6f62c2@placeholder.32106.edu. Printing is for personal, private use only. No part of this book may be reproduced or transmitted
without publisher's prior permission. Violators will be prosecuted.

Group 4
Jaramilla, Chabelita
Labininay, Angelika
PDS
Labuac, Eugene
Lechoncito, Arthur
Legaspi, Jericho

200
0
8
8
0.02581
2.06 * 10^-4
1.03 * 10^-4
5.15 * 10^-5
1.648
8.24
C. Sample Analysis
4. Volume Na2S2O3 dispensed (mL)
𝑣𝑁𝑎2 𝑆2 𝑂3 (𝑚𝐿) = 𝐵𝑢𝑟𝑒𝑡 𝑟𝑒𝑎𝑑𝑖𝑛𝑔 𝑓𝑖𝑛𝑎𝑙 − 𝐵𝑢𝑟𝑒𝑡 𝑟𝑒𝑎𝑑𝑖𝑛𝑔 𝑖𝑛𝑖𝑡𝑖𝑎𝑙

= 8 𝑚𝐿 − 0 𝑚𝐿 = 8 𝑚𝐿
6. Moles of Na2S2O3 dispensed (mol)
𝑛𝑁𝑎2 𝑆2 𝑂3 1𝐿 𝑚𝑜𝑙 𝑁𝑎2 𝑆2 𝑂3
𝑛𝑁𝑎2 𝑆2 𝑂3 (𝑚𝑜𝑙) = 𝑉𝑁𝑎2 𝑆2 𝑂3 ∗ = 8 𝑚𝐿 ∗ ∗ 0.02581
𝐿𝑁𝑎2 𝑆2 𝑂3 1000 𝑚𝐿 𝐿

= 2.06 ∗ 10−4 𝑚𝑜𝑙 𝑁𝑎2 𝑆2 𝑂3

7. Moles of I3- reduced by S2O3- (mol)
1 𝑚𝑜𝑙 𝐼3− 1 𝑚𝑜𝑙 𝐼3−
𝑛𝐼3− (𝑚𝑜𝑙) = 𝑛𝑁𝑎2 𝑆2 𝑂3 ∗ = 2.06 ∗ 10−4 𝑚𝑜𝑙 𝑁𝑎2 𝑆2 𝑂3 ∗
2 𝑚𝑜𝑙 𝑁𝑎2 𝑆2 𝑂3 2 𝑚𝑜𝑙 𝑁𝑎2 𝑆2 𝑂3
= 1.03 ∗ 10−4 𝑚𝑜𝑙 𝐼3−
8. Moles of O2 (mol)
2 𝑚𝑜𝑙 𝑀𝑛(𝑂𝐻)2 1 𝑚𝑜𝑙 𝑂2
𝑛𝑂2 (𝑚𝑜𝑙) = 𝑛𝐼3− ∗ − ∗
1 𝑚𝑜𝑙 𝐼3 4 𝑚𝑜𝑙 𝑀𝑛(𝑂𝐻)2
2 𝑚𝑜𝑙 𝑀𝑛(𝑂𝐻)2 1 𝑚𝑜𝑙 𝑂2
= 1.03 ∗ 10−4 𝑚𝑜𝑙 𝐼3− ∗ ∗
1 𝑚𝑜𝑙 𝐼3− 4 𝑚𝑜𝑙 𝑀𝑛(𝑂𝐻)2
= 5.15 ∗ 10−5 𝑚𝑜𝑙 𝑂2
9. Mass of O2 (mg)
32𝑔 𝑂2 1000𝑚𝑔
𝑚𝑂2 (𝑚𝑔) = 𝑛𝑂2 ∗ ∗
1 𝑚𝑜𝑙 𝑂2 1𝑔
32𝑔 𝑂2 1000𝑚𝑔
= 5.15 ∗ 10−5 𝑚𝑜𝑙 𝑂2 ∗ ∗
1 𝑚𝑜𝑙 𝑂2 1𝑔
= 1.648 𝑚𝑔 𝑂2
10. Dissolved oxygen, ppm O2 (mg/L)
𝑚𝑂2 1.648 𝑚𝑔
𝐷𝑖𝑠𝑠𝑜𝑙𝑣𝑒𝑑 𝑜𝑥𝑦𝑔𝑒𝑛(𝑝𝑝𝑚) = = = 8.24 𝑝𝑝𝑚
𝑉 𝑠𝑎𝑚𝑝𝑙𝑒 200 𝑚𝐿 𝑠𝑎𝑚𝑝𝑙𝑒 ∗ 1 𝐿
1000 𝑚𝐿
PRINTED BY: 1fa8ca33c6f62c2@placeholder.32106.edu. Printing is for personal, private use only. No part of this book may be reproduced or transmitted
without publisher's prior permission. Violators will be prosecuted.

FDS
Group 4
Jaramilla, Chabelita
Labininay, Angelika
Labuac, Eugene
Lechoncito, Arthur
Legaspi, Jericho

200
0
8
8
0.02581
2.06 * 10^-4
1.03 * 10^-4
5.15 * 10^-5
1.648
8.24
C. Sample Analysis
4. Volume Na2S2O3 dispensed (mL)
𝑣𝑁𝑎2 𝑆2 𝑂3 (𝑚𝐿) = 𝐵𝑢𝑟𝑒𝑡 𝑟𝑒𝑎𝑑𝑖𝑛𝑔 𝑓𝑖𝑛𝑎𝑙 − 𝐵𝑢𝑟𝑒𝑡 𝑟𝑒𝑎𝑑𝑖𝑛𝑔 𝑖𝑛𝑖𝑡𝑖𝑎𝑙

= 8 𝑚𝐿 − 0 𝑚𝐿 = 8 𝑚𝐿
6. Moles of Na2S2O3 dispensed (mol)
𝑛𝑁𝑎2 𝑆2 𝑂3 1𝐿 𝑚𝑜𝑙 𝑁𝑎2 𝑆2 𝑂3
𝑛𝑁𝑎2 𝑆2 𝑂3 (𝑚𝑜𝑙) = 𝑉𝑁𝑎2 𝑆2 𝑂3 ∗ = 8 𝑚𝐿 ∗ ∗ 0.02581
𝐿𝑁𝑎2 𝑆2 𝑂3 1000 𝑚𝐿 𝐿

= 2.06 ∗ 10−4 𝑚𝑜𝑙 𝑁𝑎2 𝑆2 𝑂3

7. Moles of I3- reduced by S2O3- (mol)
1 𝑚𝑜𝑙 𝐼3− 1 𝑚𝑜𝑙 𝐼3−
𝑛𝐼3− (𝑚𝑜𝑙) = 𝑛𝑁𝑎2 𝑆2 𝑂3 ∗ = 2.06 ∗ 10−4 𝑚𝑜𝑙 𝑁𝑎2 𝑆2 𝑂3 ∗
2 𝑚𝑜𝑙 𝑁𝑎2 𝑆2 𝑂3 2 𝑚𝑜𝑙 𝑁𝑎2 𝑆2 𝑂3
= 1.03 ∗ 10−4 𝑚𝑜𝑙 𝐼3−
8. Moles of O2 (mol)
2 𝑚𝑜𝑙 𝑀𝑛(𝑂𝐻)2 1 𝑚𝑜𝑙 𝑂2
𝑛𝑂2 (𝑚𝑜𝑙) = 𝑛𝐼3− ∗ − ∗
1 𝑚𝑜𝑙 𝐼3 4 𝑚𝑜𝑙 𝑀𝑛(𝑂𝐻)2
2 𝑚𝑜𝑙 𝑀𝑛(𝑂𝐻)2 1 𝑚𝑜𝑙 𝑂2
= 1.03 ∗ 10−4 𝑚𝑜𝑙 𝐼3− ∗ ∗
1 𝑚𝑜𝑙 𝐼3− 4 𝑚𝑜𝑙 𝑀𝑛(𝑂𝐻)2
= 5.15 ∗ 10−5 𝑚𝑜𝑙 𝑂2
9. Mass of O2 (mg)
32𝑔 𝑂2 1000𝑚𝑔
𝑚𝑂2 (𝑚𝑔) = 𝑛𝑂2 ∗ ∗
1 𝑚𝑜𝑙 𝑂2 1𝑔
32𝑔 𝑂2 1000𝑚𝑔
= 5.15 ∗ 10−5 𝑚𝑜𝑙 𝑂2 ∗ ∗
1 𝑚𝑜𝑙 𝑂2 1𝑔
= 1.648 𝑚𝑔 𝑂2
10. Dissolved oxygen, ppm O2 (mg/L)
𝑚𝑂2 1.648 𝑚𝑔
𝐷𝑖𝑠𝑠𝑜𝑙𝑣𝑒𝑑 𝑜𝑥𝑦𝑔𝑒𝑛(𝑝𝑝𝑚) = = = 8.24 mg/L
𝑉 𝑠𝑎𝑚𝑝𝑙𝑒 200 𝑚𝐿 𝑠𝑎𝑚𝑝𝑙𝑒 ∗ 1 𝐿
1000 𝑚𝐿