JABATAN KEJURUTERAAN AWAM

DCC40172
WATER QUALITY LAB

DISSOLVED OXYGEN TEST (DO)

NAME LECTURER: PUAN HANISON BINTI JUSOH

CLASS: DKA 4D

NAME NO MATRIC
MUHAMMAD ALIF ASYRAF BIN ROKHMAN 04DKA20F1042
ANIS NURDINI BINTI ROSLIMI 04DKA20F1038
NUR ZULIANA BINTI ZAMRI 04DKA20F1022

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 TABLE OF CONTENT

NO. CONTENT PAGE

1. INTRODUCTION 3
2. OBJECTIVE 3
3. EQUIPMENT 3
4. THEORY 4
5. PROCEDURE 3
6. DATA 5
7. DISCUSSION 6
8. CONCLUSION 6
9. REFERENCES 6

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INTRODUCTION
Dissolved oxygen (DO) is a measure of the quantity of free oxygen molecules in water. The
concentration of DO is an important indicator of the health of an aquatic ecosystem because
oxygen is essential for almost all forms of life. Oxygen is necessary for respiration and for
some chemical reactions. Ongoing low dissolved oxygen in a water body will harm most
aquatic life because there will not be enough to sustain life.
Conversely, too much oxygen in water (supersaturated) can also be dangerous.
Dissolved oxygen in water comes from two main sources: the atmosphere and
photosynthesis. The concentration of DO is affected by numerous variables, including water
temperature (colder water holds more oxygen than warmer water), salinity (freshwater holds
more oxygen than saltwater) and atmospheric pressure (the amount of DO absorb in water
decreases as altitude increases).

OBJECTIVE

To measure dissolved oxygen in various water samples.

EQUIPMENT

1. Sampling Bottle 2. Dissolved Oxygen Meter Test reagent:

Distilled water.

3. Beaker

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NOTE: Use only high-grade distilled or deionized water. The water must contain less than
0.01 mg/L copper, and be free of chlorine, chloramines, caustic alkalinity, organic material,
or acids

THEORY

The dissolved oxygen (DO) is oxygen that is dissolved in water. It can be measured with a
dissolved oxygen meter. The standard unit is milligrams per litre (mg/l) or parts per million
(ppm).

Oxygen present in water by diffusion from the air, by aeration, from photosynthesis. Total
dissolved gas concentrations in water should not exceed 110 percent. This is because it could
be harmful to aquatic life. Fish in waters containing excessive dissolved gases may suffer from
"gas bubble disease" however; this is a very rare occurrence.

Dissolved oxygen is used by organic material for decomposition, and is dictated by the
temperature, salinity, and the atmospheric pressure.

PROCEDURE
1. Using pail or sampling bottle, collect water samples from selected sources such as lake,
river, or drain into any appropriate sample container, avoid aerating the sample.

2. Pour the water sample into beaker.

3. Place the probe in the sample, allow the meter to equilibrate, and read the DO
concentration directly from the meter.

NOTE: The probe may need to be gently stirred to aid water movement across the membrane.

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DATA
Sample Reading 1 Reading 2 Reading 3 Average
1 5.9 6.3 6.1 6.1

2 6.9 6.8 7.0 6.9

7.3 7.3 7.6 7.4
3
8.0 7.7 8.0 7.9
4
8.4 8.0 8.5 8.3
5

Table 1: Dissolved Oxygen Average

Calculations

Sample 1 = 5.9 + 6.3 + 6.1 = 6.1 mg/L

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Sample 2 = 6.9 + 6.8 + 7.0 = 6.9 mg/L
3
Sample 3 = 7.3 + 7.3 + 7.6 = 7.4 mg/L
3
Sample 4 = 8.0 + 7.7 + 8.0 = 7.9 mg/L
3
Sample 5 = 8.4 + 8.0 + 8.5 = 8.3 mg/L
3

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DISCUSSION

Dissolved oxygen (DO) is one of the important indicators of water quality. Oxygen dissolves
in surface water due to the action of wind ventilation. When dissolved oxygen becomes too
low, fish and other aquatic organisms cannot survive. Water Quality Index based on the
National Water Quality Standard, the range of DO value is between 3 to 7. DO value 7 which
is class I indicates clean water quality. DO values between 7 to 3 class II and III indicate
good water quality. Next if they DO value reading is less than 3 class IV and V it indicates
contaminated water quality. (Water.gov.my)
The results of the analysis found that the DO values for the samples were different.
The lowest reading was recorded by sample 1 which was 6.1 mg/L while the highest reading
was sample 5 which was 8.3 mg/L. This shows that the overall average Dissolved Oxygen
(DO) for the river is between 6 to 8. Therefore, it can be said that the river water is still in a
safe condition and not too dangerous to life.

CONCLUSION

Based on the data, the mean DO values for samples 1, 2, 3, 4 and 5 were 6.1, 6.9, 7.4, 7.9 and

8.3, respectively. The acceptable limit of Dissolved Oxygen in water is 3 mg/L to 7 mg/L.
Water with a DO of less than 3 mg/L is very dangerous to drink. While water with a DO
value of 7 is the cleanest because it is in class I. Therefore, sample 2 is the safest and cleanest
compared to other samples because the value is closest to 7.

References

1. Chegg.com, Solved A test was used to determine oxygen transfer, from:

https://www.chegg.com/homework-help/questions-and-answers/test-used-determine-
oxygen-transfer-coefficient-kl-sea-level-170-m-water-temperature-20-de-q20077085
2. Puan Yuslina. Pengukuran kualiti air sungai berdasarkan jps river index (jri) di
lembangan sungai klang.water.gov.my, from:
https://www.water.gov.my/jps/resources/auto%20download%20images/5848ebac0e6
51.pdf