A.

PREPARATION OF THE REAGENT FLASK

PREPARE A BROMINE REAGENT

FLASK FROM A 250 mL
S ERLENMEYER FLASK.
CAUTION!

T FIT THE FLASK WITH A

Do not get Br-HAc solution on
your skin. If this happens,
E MEDICINE DROPPER IN A
HOLE CORK OR RUBBER
wash it off immediately, since
it will never cause burns.
P STOPPER.

S FILL WITH 50 ML OF 5%
BROMINE-ACETIC SOLUTION.

PRINCIPLE: The excess bromine not taken up by the unsaturated bonds

displaces an equimolar quantity of iodine to liberate iodine.
 B. PREPARATION OF REACTION FLASK

PLACE 0.2 TO 0.3 GRAM

FAT OR OIL IN ANOTHER
S FLASK.

T LIQUIDS MAY BE ADDED WITH A

DROPPER AND SOLID WITH A
E WOODEN SPLIT SPATULA. BE SURE
THAT SOLIDS ARE AT THE BOTTOM
P
S DISSOLVE ALL OF THE FAT OR
OIL IN 0.5 mL OF ETHER BY
STIRRING WITH A GLASS ROD.

PRINCIPLE
 C. PREPARATION OF THE BLANK FLASK

USE ANOTHER 250 mL

S ERLENMEYER FLASK.

T
PUT 10 mL ETHER & 10
E DROPS OF BROMINE-ACETIC
ACID SOLUTION.
P
S STOPPER THE FLASK AND
PLACE ON WHITE PAPER.

PRINCIPLE
 D. DETERMINATION OF THE IODINE NUMBER

WEIGH THE REAGENT FLASK THEN

THENFILL
FILLTHETHE
DROPPER WITH
DROPPER
START TO TWO DECIMAL PLACE AND BROMINE SOLUTION FROM
S RECORD THE WEIGHT WITHTHEBROMINE SOLUTION
REAGENT FLASK
FROM THE REAGENT FLASK
COLOR SHOULD RAPIDLY ADD TO THE REACTION
T PERSIST FOR ABOUT
HALF A MINUTE.
CONTINUE UNTIL THE COLOR IN
THE REACTION FLASK MATCHES
THE COLOR OF THE BLANK.
FLASK (SQUIRT IN ABOUT 10 D.
AT A TIME & SWIRL THE FLASK.
E RETURN EXCESS BROMINE WEIGH THE THE DIFFERENCE BET. THE INITIAL
SOLUTION FROM THE DROPPER REAGENT AGAIN & FINAL WEIGHT OF TEH REAGENT
P TO REAGENT FLASK. AND RECORD. FLASK EQUALS THE...

S END
REPEAT DETERMINATION
3 TIMES IF
PRECISION IS POOR.
POUR THE CONTENT INTO RECEPTABLE FOR
WASTE SOLVENTS, WASH AND DRY THE
FLASK BEFORE WEIGHING ANOTHER OIL.
...THE WEIGHT OF THE
BROMINE SOL'N USED
IN THE REACTION.

The determination of the amount of unsaturation contained in the

fatty acids. This unsaturation is in the form of double bonds
PRINCIPLE: which reacts with iodine compounds. The > the iodine value, the
more unsaturated fatty acids bonds are present in the fat.