NUEVA ECIJA UNIVERSITY OF SCIENCE AND TECHNOLOGY

COLLEGE OF ARTS AND SCIENCES

COLLEGE OF NURSING
BIOCHEMISTRY (MC2) LABORATORY
POST LABORATORY REPORT IN

PROPERTIES OF LIPIDS
ROXANNE S. ECLEVIA
BSN 1 – A
OCTOBER 29, 2019

INTRODUCTION

Lipids are organic compounds that contain hydrogen, carbon, and oxygen atoms, which forms the

framework for the structure and function of living cells. These organic compounds are nonpolar molecules,

which are soluble only in nonpolar solvents and insoluble in water because water is polar molecules. In the

human body, these molecules can be synthesized in the liver and are and generally found in the oil, butter,

whole milk, cheese, fried foods, and also in some red meats. The difference between fats and oils is on the

basis of their physical states at room temperature. It is customary to call a lipid a fat if it is solid at 25°C, and an

oil if it is a liquid at the same temperature.

It is established that lipids play extremely important roles in the normal functions of a cell. Not only do

lipids serve as highly reduced storage forms of energy, but they also play an intimate role in the structure of

cell membrane and organellar membranes.  Lipids perform many functions, such as: Energy Storage, Making

Biological Membranes, Insulation, Protection – e.g. protecting plant leaves from drying up, Boyancy, Acting as

hormones, Act as the structural component of the body and provide the hydrophobic barrier that permits

partitioning of the aqueous contents of cell and sub cellular structures. Lipids are major sources of energy in

animals and high lipid-containing seeds and activators of enzymes.

METHODS

IN SEPARATE TEST TUBES

PLACE 1 ML OF WATER,
DILUTE HCl, DILUTE + 2 DROPS OF EACH TEST OBSERVE IN WHAT
NaOH, ETHYL ALCOHOL, SAMPLE IN EACH TEST SOLVENT IS THE OIL
CHLOROFORM, ETHER TUBE SOLUBLE.
AND CARBON
TETRACHLORIDE

Figure 1. Solubility Test

ON THE OTHER SIDE OF AFTER 15 MINS, NOTE

THE SAME FILTER PAPER, THE APPERANCE OF THE
PUT A DROP OF TEST
PUT A DROP OF ETHYL FILTER PAPER. NOTE
SOLUTION ON ONE SIDE
ALCOHOL AND ON THE WHICH WILL LEAVE A
OF FILTER PAPER.
OTHER CORNER A DROP SPOT AND WHICH WILL
OF ETHER. LABEL EACH. NOT.

POST LABORATORY REPORT IN BIOCHEMISTRY (MC2) rtae

 Figure 2. Spotting Effect

PLACE 1 ML OF ETHYL
TEST THE SOLUTION
ALCOHOL IN SEPARATE
WITH RED AND BLUE
TEST TUBES + 5 DROPS
LITMUS PAPER
OF TEST SAMPLES

Figure 3. Reaction with Litmus Paper

PLACE 1 ML OF TEST HEAT IN WATER BATH

+ A PINCH OF
SAMPLE IN SEPARATE OBSERVE AND DESCRIBE
POTASSIUM BISULFATE
TEST TUBES THE ODOR PRODUCED

Figure 4. Acrolein Test

+ DROPWISE OF A
SOLUTION OF Br2 IN CCl4
PLACE 5 DROPS OF TEST
TO EACH, COUNT THE
SAMPLES IN 1ML OF
NO. OF DROPS REQUIRED
CHLOROFORM
TO PRODUCE REDDISH
SOLUTION

Figure 5. Test for Unsaturation

+ 15 DROPS OF ACETIC
PLACE 5 DROPS OF TEST ANHYDRIDE, MIX
SAMPLES AND + MIX AND NOTE THE
CHLOROFORM UNTIL IT IS + 5 DROPS OF COLOR CHANGES
DISSOLVED CONCENTRATED
SULFURIC ACID

Figure 6. Liebermann-Burchard Test

RESULTS

TEST DILUTE DILUTE ETHYL CHLOROFORM ETHER CARBON WATER

SAMPLE HCl NaOH ALCOHOL TETRACHLORIDE
Butter Not Not Not Soluble Soluble Soluble Not
Soluble Soluble Soluble Soluble
Canola Not Not Not Soluble Soluble Soluble Not
Soluble Soluble Soluble Soluble
Coconut Not Not Not Not Soluble Soluble Soluble Not
Soluble Soluble Soluble Soluble
Palm Not Not Not Soluble Soluble Soluble Not
Soluble Soluble Soluble Soluble
Lauric Not Not Not Soluble Soluble Soluble Not
Soluble Soluble Soluble Soluble
Table 1. Solubility Test Results

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 TEST SAMPLE SPOTTING LIEBERMANN- REACTION WITH ACROLEIN TEST
EFFECT BURCHARD TEST LLITMUS PAPER
Butter Spot is Visible DARK GREEN BLUE: No No burnt smell
(Change in Color)Changes
RED: No Changes
Canola Spot is Visible GREEN (Change BLUE: No No burnt smell
in Color) Changes
RED: No Changes
Coconut Spot is Visible GREEN (Change BLUE: No No burnt smell
in Color) Changes
RED: No Changes
Palm Spot is Visible DARK GREEN BLUE: No No burnt smell
(Change in Color) Changes
RED: No Changes
Lauric Spot is Visible GREEN (Change BLUE: No No burnt smell
in Color) Changes
RED: No Changes
Water Not Visible GREEN (Change BLUE: No No burnt smell
in Color) Changes
RED: No Changes
Table 2. Results of other tests performed.

CONCLUSION

DISCUSSION AND INTERPRETATION OF THE DATA GATHERED IN RELATION TO THE GIVEN

BACKGROUND IN THE INTRODUCTION MUST BE REFLECTED IN THIS PART. A LOT ONE PARAGRAPH

EACH FOR EVERY PART OF THE EXPERIMENT. THERE IS NO LIMITATION AS TO HOW MANY

SENTENCES MAY BE USED. BE COHERENT IN CONSTRUCTING YOUR CONCLUSION.

REFERENCE:

To

https://microbenotes.com/lipids-properties-structure-classification-and-functions/

https://byjus.com/biology/lipids/

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