RESURRECCION, CYNTHIA B.

GROUP 6 DMD-1I
ROQUEN, ELLAINE KATE TTh 7:00-10:00 AM
SICAT,MIHO MRS. BERONIO
SEPTEMBER 20, 2016

EXPERIMENT 9

PROPERTIES OF PROTEINS

A. REACTION WITH HEAT

SAMPLE OBSERVATION
ALBUMIN Formation of white coagulate
GELATIN Formation of colorless coagulate
CASEIN Formation of white coagulate

EXPLANATION:

 Gentle heating causes reversible denaturation of protein

 Vigorous heating denatures protein irreversibly by disrupting several types of bonds.
 Egg white, contains high percentage of protein, coagulates on heating
 Heat coagulates and destroys protein present in bacteria- thus, sterilization of instruments and
clothing use in operating rooms requires the use of high temperatures
B. REACTION WITH SALTS OF HEAVY METALS

SAMPLE REAGENT OBSERVATION

ALBUMIN +AgNO3 more white coagulate is formed
ALBUMIN +Hg2Cl2 less white coagulate is formed

EXPLANATION:

 *Heavy metal salts leads to the precipitation of protein. It also cleaves -SH bonds.
 Denatured protein irreversibly by disrupting the salt bridges and the disulfide bonds present
in the protein.
 It is poisonous if taken internally because they coagulate and destroy proteins present in the
body.
C. REACTION WITH ALKALOIDAL REAGENTS

SAMPLE REAGENT OBSERVATION

ALBUMIN + Sat. Picric acid There is a formation of suspended colloids and it is
color yellow coagulate therefore coagulation happens.
ALBUMIN + Tannic acid There is a formation of suspended colloids and it is
brown/flesh coagulate therefore coagulation happens.
ALBUMIN + TCA There is a formation of suspended colloids and it is
color white/ milky white coagulate therefore
coagulation happens.

*All reagents are clear solution.

 Sat. Picric acid – yellow clear solution
 Tannic acid - brown clear solution
 TCA- white clear solution

EXPLANATION:

 Alkaloidal reagents, such as tannic acis and picric acid form insoluble compounds with proteins
 Alkaloidal reagents denature protein irreversibly by disrupting salt bridges and hydrogen bond
D. REACTION WITH CONCENTRATED ACIDS

SAMPLE REAGENT OBSERVATION

ALBUMIN + HNO3 There is a formation of coagulation and its color is
yellow opaque.
+ EXCESS HNO3 Yellow coagulate disappears
ALBUMIN + HCl There is a formation of coagulation and its color is
white opaque.
+ EXCESS HCl White coagulate disappears
* 2 drops of each reagent

EXPLANATION:

 Changes in pH can disrupt hydrogen bonds and salt bridges, causing irreversible denaturation.
 Proteins are coagulates by strong acids as concentrated HCl, sulfuric and nitric acid.
E. REACTION WITH ALCOHOL

SAMPLE REAGENT OBSERVATION

ALBUMIN + 95 % ethyl alcohol There is coagulation in the middle region of the
solution and it is white opaque. There is a greatest
amount of white coagulate formed.
ALBUMIN + 70 % ethyl alcohol There is coagulation in the middle and bottom region
of the solution and it is less white opaque. There is a
moderate amount of white coagulate formed.
ALBUMIN + 45 % ethyl alcohol There is a formation of precipitate in the bottom and it
is white translucent. There is a least amount of white
coagulate formed.

EXPLANATION:

 Alcohol coagulates (precipitates) all types of proteins except prolamines

 Alcohol denatures protein by forming hydrogen bonds that compete with the naturally occurring
hydrogen bonds in the proteins
 Process is not reversible
 70 % alcohol- used to distinct because of its ability to coagulate the proteins present in the bacteria