Beckman Coulter Chemistry Analyzer: Ise User Guide

BECKMAN COULTER
CHEMISTRY ANALYZER
AU480
ISE USER GUIDE
© 2009 Beckman Coulter Biomedical K.K.. All Rights Reserved.
Beckman Coulter AU480 User Guide.
This User Guide as well as the system described in it may be used or copied only in accordance with
the terms of copyright. The content of this User Guide is intended for instructional use only and is
subject to change without notice.
No part of this publication may be reproduced, stored in a retrieval system, or transmitted, in any form
or by any means, electronic, mechanical or otherwise, without the prior written permission of Beckman
Coulter Biomedical K.K..
Please remember that existing artwork or images from this guide that you may want to include in your
project may be protected under copyright law.
The unauthorized incorporation of such material into your new work could be a violation of the rights of
the copyright owner. Please be sure to obtain any permission required from the copyright owner.
Any references to any company names or peoples names in sample templates and windows are for
demonstration purposes only and are not intended to refer to any actual organization or names of
persons.
Beckman Coulter Biomedical Limited.

Lismeehan, O’Callaghan’s
Mills, Co. Clare,
EC REP
Ireland
Beckman Coulter Biomedical Limited. is the EC authorized representative of:
Beckman Coulter Biomedical K.K.

TOC Ariake West Tower,
3-5-7, Ariake,
Koutou-ku, Tokyo, 135-0063,
Japan
Beckman Coulter Mishima K.K.

454-32 Higashino,
Nagaizumi-cho,
Sunto-gun, Shizuoka, 411-0931,
Japan
Lumi-Phos ® 530 is a registered trademark of Lumigen, Inc., Southfield, Michigan, USA.

Windows ® is either a registered trademark or trademark of Microsoft Corporation in the United States
and/or other countries.
Adobe Acrobat® Reader ® is either a registered trademark or trademark of Adobe Systems
Incorporated in the United States and/or other countries.
Manufacturer Authorized Representative in the European

EC REP Community
Return to Main Table of Contents
Document Change Notification
Document Software Date ISE User Guide change description

Revision Version
Number
1 1.0 2009/10/25 New manual
DCN-1
ISE User Guide
Version 1.0
DCN-2
ISE User Guide
Version 1.0
FORWARD
Thanks for using the optional ISE unit for Beckman Coulter Automated Chemistry Analyzers.
This ISE User Guide (Supplement) offers detail descriptions, limited troubleshooting to common
problems, and maintenance procedures to be used in conjunction with the conventional user guide.
Use also the conventional user guide.
Precautions in Using This Manual

• Before getting started with this system, read this manual thoroughly along with the conventional
user guide to understand the contents.
• The contents of this manual are subject to change without prior notice.
• No part or whole of this manual may absolutely be copied or reproduced in any form without the
written permission of Beckman Coulter.
• Carefully store this manual and the conventional user guide for future reference.
• For any questions, mistakes or omissions detected in this manual, contact Beckman Coulter Sales
or Beckman Coulter Technical Service.
Whom and What This Manual is for
This manual is intended for laboratory personnel, and explains troubleshooting techniques and
maintenance procedures on a day-to-day basis for the ISE unit of the AU480.
Objective readers
This manual is intended for laboratory personnel who have completed the training course offered by
Beckman Coulter, or personnel who have been trained by those who have taken Beckman Coulter
training course.
Aim of this manual

In order to use the optional unit ISE safely and under optimal conditions, the aim of this manual is to
familiarize the readers thoroughly with the basic maintenance operations.

ISE User Guide
Version 1.0
How AU480 Documents are Organized
AU480 ISE
User Guide User Guide
This manual
How This Manual is Structured

This manual is structured into two parts.
Part I: ISE TROUBLESHOOTING

This part gives troubleshooting information in flowchart form about some problems that may occur in
client workflows.
Part II: ISE MAINTENANCE FLOW

This part gives the procedures of periodic maintenance and corrective maintenance in
case of ISE trouble, which should be performed by clients.
ii
ISE User Guide
Version 1.0
Contents
Document Change Notification ...................................................................................Behind Cover Page
Part I ISE TROUBLESHOOTING I-1

1 ISE WORKFLOWS AND PROBLEMS THAT CAN OCCUR....................................... I-2
2 OTHER ERRORS IN ISE............................................................................................. I-3
3 TROUBLESHOOTING................................................................................................. I-4
Part II ISE MAINTENANCE FLOW II-1

1 ISE MAINTENANCE CHECKLIST.............................................................................. II-2
2 OVERVIEW OF ISE MAINTENANCE FLOW.............................................................. II-4
3 LIST OF PARTS USED FOR ISE MAINTENANCE.................................................... II-5
4 TUBING BLOCK DIAGRAM FOR ISE MAINTENANCE............................................ II-7
5 DAILY MAINTENANCE............................................................................................... II-8
5.1 Check the Buffer Syringe for Leaks......................................................... II-8
5.2 Auto-wash the Sample Pot and Electrode Line....................................... II-10
6 WEEKLY MAINTENANCE........................................................................................... II-12
6.1 Check the Selectivity of the Na and K Electrodes................................... II-12
6.2 Enhanced Cleaning of Electrode Line..................................................... II-14
7 MAINTENANCE REQUIRED EVERY TWO WEEKS OR 3,000 SAMPLES............... II-16
7.1 Manually Wash the Mix Bar and Liquid Level Sensors............................ II-16
7.2 Manually Wash the Sample Pot and the Sample Pot Tubes................... II-18
8 MAINTENANCE REQUIRED EVERY TWO MONTHS OR 20,000 SAMPLES........... II-22
8.1 Replace the Na, K, or Cl Electrode.......................................................... II-22
8.2 Check and Add REF Electrode Solution.................................................. II-25
9 MAINTENANCE REQUIRED EVERY THREE MONTHS OR 20,000 SAMPLES....... II-26
9.1 Replace the Roller Tubes for MID Solution Dispense
and Mixture Aspiration............................................................................. II-26
9.2 Replace the Sample Pot Tubes............................................................... II-29
9.3 Replace the REF Block Side Drain Tube and the Pinch Valve Tubing.... II-32
9.4 Replace the Drain Tube and Manually Wash the Drain Tank.................. II-35
10 MAINTENANCE REQUIRED EVERY TWO YEARS OR 150,000 SAMPLES............ II-38
10.1 Replace the REF Electrode and Packing................................................ II-38
11 MAINTENANCE ON AN AS-NEEDED BASIS............................................................ II-42
11.1 Replace the Sample Pot.......................................................................... II-42
11.2 Clean the Electrode Block (Inlet Side)..................................................... II-44
11.3 Manually Wash the K Electrode............................................................... II-47
11.4 Manually Wash and Replace the REF Electrode Block........................... II-49
11.5 Replace Reagents................................................................................... II-53
11.6 Replace the Buffer Syringe...................................................................... II-55
11.7 Procedure for Enhanced Cleaning of the Electrode Line (Manual)......... II-58
11.8 Replace the Buffer Syringe Case and Case Head................................... II-61
12 RESETTING THE SYSTEM WHEN SWITCHED TO THE STOP MODE
WHILE PERFORMING MAINTENANCE..................................................................... II-64

ISE User Guide Contents
Version 1.0

Contents ISE User Guide
Version 1.0
Part I
ISE TROUBLESHOOTING
In order to keep the ISE unit of the AU480 in good condition and to
maintain it’s original performance, customers should perform periodic
maintenance.
This part of “ISE TROUBLESHOOTING” describes problems that can
occur and how to resolve them in flow chart form.
Also refer to “Part II MAINTENANCE FLOW”.
Contents
1 ISE WORKFLOWS AND PROBLEMS THAT CAN OCCUR...........................................I-2
2 OTHER ERRORS IN ISE..................................................................................................I-3
3 TROUBLESHOOTING.....................................................................................................I-4
I-1
ISE User Guide ISE TROUBLESHOOTING
Version 1.0
1 ISE WORKFLOWS AND PROBLEMS THAT CAN

OCCUR
Workflow Problems Encountered Remedies
(1) ISE sample pot liquid level

Power ON sensor error -1
(3186, 3187)
Refer to page I-4.
Notifies that the diluent pot is
overflowing with the diluent.
Calibration
(3) Calibration error

Refer to pages
Notifies that the slope factor I-6 and I-7.
has exceeded the rated value.
Analysis Operation
Start (2) ISE sample pot overflow
sensor error (3186)
Notifies that the liquid level

Refer to page I-5.
sensor in the diluent pot does
not function properly.
(4) Concurrent
reproducibility error Refer to page I-8.
(5) Daily variation Refer to page I-9.

sensor error -1 Refer to page I-4.
(3186, 3187)
(6) ISE data low-value

(3188, 3509)
Refer to page I-10.
Notifies that in the Serum
mode the Na measurements
of three serial samples are
less than 70mmol/L.
Maintenance
(7) Selectivity check error Refer to page I-11.

sensor error -1 Refer to page I-4.
(3186, 3187)
Power OFF
If another error occurs: Refer to page I-3.
I-2
ISE TROUBLESHOOTING ISE User Guide
Version 1.0
2 OTHER ERRORS IN ISE

If any of the following errors are encountered, contact Beckman Coulter
Technical Services.
These Errors indicate unit malfunctions
Alarm No. Display
3182 ISE Buffer Dispenser (SA-ISE) Error
3183 ISE Mixture Rolling Pump (IB-S) Error
3184 ISE MID Rolling Pump (IB-M) Error
3185 ISE Mixture Unit (ISE-MIX) Error
3189 ISE Analog Power Supply Error
These Errors indicate abnormal communication conditions

Alarm No. Display
3032 ISE Control Error 1
3033 ISE Control Error 2
3180 ISE sequence error
3181 ISE A/D error
If any of the following errors are encountered, check the level of the solution
named in the Display column next to the corresponding Alarm No. Column.
These Errors indicate insufficient reagent liquid level
Alarm No. Display
3251 ISE Buffer Solution Short
3252 ISE MID Solution Short
3253 ISE REF Solution Short
3254 ISE Detergent Short
3255 ISE Serum Standard Solution H Short
3256 ISE Serum Standard Solution L Short
3257 ISE Urine Standard Solution H Short
3258 ISE Urine Standard Solution L Short
3259 ISE Electrode Check Liquid (Na) Short
3260 ISE Electrode Check Liquid (K) Short
3261 ISE CRS Standard Solution H Short
3262 ISE CRS Standard Solution M Short
3263 ISE CRS Standard Solution L Short
I-3
Version 1.0
3 TROUBLESHOOTING
(1) Troubleshooting for ISE Sample Pot Liquid level Sensor Error
-1 (3186, 3187)
3186(1)
3187(1) ISE sample pot liquid level sensor error – 1
3187(2)
Does liquid remain Does the liquid level sensor and

in the diluent pot? its surrounding area get wet or dirty?
Contact Beckman Coulter Technical Services.
CALL (The liquid level sensor is
NO NO YES supposed to be defective.)
YES YES
Clean the liquid level sensor.
Part II 7.1
Is the mixing unit installed properly?
Properly install the mixing unit.

NO
YES
Is any tubing detached?
Reattach the tubing in place.

YES
NO
Is there any dirty, bent,
crashed, or leaking tubing?
Wash the tubing.

Part II 5.2, 7.2, 11.2, 11.4
YES
NO
Replace the tubing.
Part II 9.1, 9.2, 9.3, 9.4
Perform user maintenance prime operation.
Does aspiration operate properly?
CALL Contact Beckman Coulter Technical Services.

NO
YES
END
Details of operating
Check item Cause and action Service call
procedure
CALL
I-4
Version 1.0
(2) a Troubleshooting for ISE Sample Pot Overflow Sensor Error

(3186)
3186(2) ISE sample pot overflow sensor error
Has the buffer solution been

dispensed up to the sensor position?
Continue to
“(2) b Troubleshooting for ISE Sample
YES Pot Liquid level Sensor Error - 2 (3186)”
NO
Is any tubing detached?
Reattach the tubing in place.

YES
NO
Check the liquid level of the buffer solution.
Check the ISE buffer syringe.

Part II 5.1
Has the problem been corrected?

CALL (The dispense nozzle is
NO
supposed to be defective.)
YES
END
(2) b Troubleshooting for ISE Sample Pot Liquid level Sensor Error
-2 (3186)
3186(3) ISE sample pot liquid level sensor error – 2
Is the level sensor connector detached?
Remove the liquid level sensor connector and reconnect it.

NO
YES
Connect the level sensor connector.

Contact Beckman Coulter
CALL
Technical Services.
NO
YES
END
procedure
CALL
I-5
Version 1.0
(3) Troubleshooting for Calibration Error – 1 of 2 (3264, 3265,

3266, 3267, 3268, 3269)
START Calibration error
Is the probe factor 0 for all items?
Continue “Troubleshooting for

NO Calibration Error – 2 of 2”.
YES
Has the electrode set properly?

Re-set the electrode and check for
the connection of lead wires.
Part II 8.1
NO
YES
Has the reagent/standard solution set properly?
Re-set the reagent/standard solution.

NO
YES
Has the buffer solution been dispensed properly?
Check the ISE buffer syringe.

Part II 5.1
NO
YES
Check/replace the roller tube.
Part II 9.1

CALL (The dispense nozzle/roller pump is
NO supposed to be defective.)
Are air bubbles YES
in the tubing
on the electrode END
outlet side?
Check the O-ring of the indicated
electrode.
Part II 8.1
YES
NO
Check the REF electrode packing.
Part II 10.1
NO
YES
Are air bubbles
gotten in the REF END
solution tubing?
CALL Contact Beckman Coulter Technical Services .

YES
NO
Is the REF electrode filled with solution?
Add the internal solution
into the REF electrode.
Part II 8.2
NO
YES
The sample probe gets dirty or clogged.
→Wash or replace the sample probe.
User Guide 8.3.4, 8.8.4
The tubing gets dirty.

→Wash or replace the tubing.
Part II 5.2, 7.2, 9.1, 9.2, 9.3, 9.4, 11.2, 11.4
The indicated electrode/REF electrode has deteriorated.

→Replace the electrode.
Part II 8.1, 10.1

CALL
NO (The electric system is supposed to be defective.)
YES
END
I-6
Version 1.0
(3) (Continued) Troubleshooting for Calibration Error – 2 of 2

Continued from
Is the slope of the specific item 0?
“Troubleshooting for The REF solution has been
Calibration Error – 1 of 2”. entered into the electrode/tubing.
Perform MID/REF solution prime.
YES
NO →Wash or replace the tubing.
Part II 5.2, 7.2, 9.1, 9.2, 9.3, 9.4, 11.2, 11.4
The REF solution has been
entered into the K electrode
→Wash the K electrode.
Part II 11.3
The indicated electrode has deteriorated.

Part II 8.1

CALL (The electric system is
NO supposed to be defective.)
YES
END
Does the slope indicate an abnormal value?
Replace the standard solution.

YES The indicated electrode
NO has deteriorated.
Part II 8.1

CALL (The electric system is
NO
supposed to be defective.)
YES
END
Does the factor indicate an abnormal value?

The standard solution has
been concentrated or diluted.
Replace the standard solution.
YES
NO The MID solution has
been concentrated or diluted.
Replace the MID solution.

→Wash or replace the sample probe.
User Guide 8.3.4, 8.8.4
The sample syringe is leaked or abraded.
→Replace the sample syringe.
Contact User Guide 8.8.7
CALL Beckman Coulter The ISE buffer syringe is leaked or abraded.
Technical Services. →Replace the ISE reagent syringe.
Part II 5.1, 11.6
The REF solution has been mixed into
the buffer solution/MID solution
(this occurred at the K/Cl electrode).
Replace the reagent.

CALL (The reagent dispense nozzle/tubing is
NO supposed to be leaked.)
YES
END
procedure
CALL
I-7
Version 1.0
(4) Troubleshooting for Concurrent Reproducibility Error

START Concurrent reproducibility error
Reproducibility has deteriorated

for the Na, K, and Cl electrodes.
Reproducibility has deteriorated for the specific item.

NO
YES The REF solution has been
entered into the electrode/tubing. Perform MID/REF solution prime.
Part II 5.2, 7.2, 9.1, 9.2, 9.3, 9.4, 11.2, 11.4
The REF solution has been entered into the K electrode.
→Wash the K electrode.
Part II 11.3
The electrode has deteriorated.
Part II 8.1

CALL (The electric system is supposed to be
NO
defective.)
YES
END
This error results The sample probe gets dirty.

from sample dispense. →Wash or replace the probe.
User Guide →8.3.4, 8.8.4
The sample syringe leaks.
→Replace the syringe.
User Guide →8.8.7
This error results from The tubing system is clogged or leaking.
buffer solution dispense. →Check the ISE buffer syringe.
Part II 5.1
The error results from The mix bar comes in contact with the diluent pot.
mixing. →Check the mix bar.
Part II 7.1
The error results from The sample pot gets dirty, clogged or leaks.
the diluent pot. →Wash or replace the sample pot.
Part II 7.2, 9.2, 11.1
MThis error results from The tubing system is clogged or leaks.
MID solution dispense. →Check the roller tube.
Part II 9.1
The error results Air enters into the REF solution tubing.
from the tubing. →Replace the electrode set and O-ring.
Part II 8.1
The tubing get clogged, dirty, or leaks.
Part II 5.2, 7.2, 9.1, 9.2, 9.3, 9.4, 11.2, 11.4
The error results from Air enters into the REF solution tubing.
the REF solution tubing. →Replace the REF electrode packing.
Part II 10.1
The amount of the REF electrode solution is insufficient.
→Add the REF electrode solution.
Part II 8.2
The indicated electrode/REF
The error results electrode has deteriorated.
from electrodes. →Replace the electrode.
Part II 8.1, 10.1

NO
- Reagent dispense nozzle
YES - Roller pump
- Pinch valve
- REF solution tubing
END - REF valve
- The electric system, etc. are supposed to be defective.
procedure
CALL
I-8
Version 1.0
(5) Troubleshooting for Daily Variation

START Daily variation in the control sample
Is the concurrent reproducibility favorable?
Refer to the flowchart of “Concurrent reproducibility error”

NO on the previous page.
YES
Do the measurement values increase for all items?
The control sample has been concentrated.
Replace the control sample.
YES
Fluctuation of the room temperature
NO Refer to
(±2°C or more from the analysis start) Adjust the room temperature.
Perform calibration again. “Troubleshooting for
The sample probe gets dirty. Calibration Error”.
→Wash or replace the probe.
User Guide 8.3.4, 8.8.4
The ISE buffer syringe is loosened or leaks.

→Tighten or replace the syringe.
Part II 5.1
NO
YES
END
Do the measurement values decrease for all items?

The control sample is diluted.
Replace the control sample.
YES
NO Fluctuation of the room temperature
(±2°C or more from the analysis start) Adjust the room temperature. Refer to
Perform calibration again. “Troubleshooting for
Calibration Error”.
The sample probe gets clogged.
→Wash or replace the probe.
User Guide 8.3.4, 8.8.4
The sample probe is loosened or leaks.
→Tighten or replace the probe.
User Guide 8.8.4
NO
YES
Does a measurement
value increase only END
for the K electrode?
NO
YES Did the control sample
analyze next to the
highvalue sample?
The indicated electrode has deteriorated.
Part II 8.1
NO
YES Has the problem been corrected?
NO
YES
END
The sample pot gets dirty or clogged.

→Wash the pot.
Part II 5.2, 7.2
Part II 5.2, 7.2, 9.1, 9.2, 9.3, 9.4, 11.2, 11.4
The roller tube has deteriorated.
→Replace the tube.
Part II 9.1, 9.2, 9.3, 9.4
Has the problem corrected?
NO
YES
END
I-9
Version 1.0
(6) Troubleshooting for ISE Data Low-value (3188, 3509)

3188, 3509 ISE data low-value
Check the sample.
Has calibration been performed properly?

Perform calibration again. Refer to “Troubleshooting
NO for Calibration Error”.
YES
The sample syringe is loosened or leaks.

→Replace the syringe.
User Guide 8.8.7

→Wash the sample probe
or replace it.
User Guide 8.3.4, 8.8.4
Replace the electrode.

Part II 8.1

NO
YES
END
procedure
CALL
I-10
Version 1.0
(7) Troubleshooting for Selectivity Check Error

START Selectivity check error
Has the check solution set properly?
Replace the check solution.

NO
YES
Has calibration been performed properly?

Perform calibration again. Refer to “Troubleshooting
NO for Calibration Error”.
YES
Replace the electrode.

Part II 8.1

NO
YES
END
procedure
CALL
I-11
Version 1.0
I-12
Version 1.0
Part II
ISE MAINTENANCE FLOW
In order to maintain the original performance of the AU480 ISE unit
and to keep it operating in good condition users should perform
periodic maintenance on the analyzer.
This part of “ISE MAINTENANCE FLOW” describes the daily,
periodic and occasional maintenance procedures to be performed in
case of trouble along with a series of operation flows, based on the
conventional ISE User Guide.
Also refer to “Part I ISE TROUBLESHOOTING”.
Contents
1 ISE MAINTENANCE CHECKLIST..................................................................................II-2
2 OVERVIEW OF ISE MAINTENANCE FLOW..................................................................II-4
3 LIST OF PARTS USED FOR ISE MAINTENANCE........................................................II-5
4 TUBING BLOCK DIAGRAM FOR ISE MAINTENANCE................................................II-7
5 DAILY MAINTENANCE...................................................................................................II-8
6 WEEKLY MAINTENANCE............................................................................................II-12
7 MAINTENANCE REQUIRED EVERY TWO WEEKS OR 3,000 SAMPLES.................II-16
8 MAINTENANCE REQUIRED EVERY TWO MONTHS OR 20,000 SAMPLES.............II-22
9 MAINTENANCE REQUIRED EVERY THREE MONTHS OR 20,000 SAMPLES.........II-26
10 MAINTENANCE REQUIRED EVERY TWO YEARS OR 150,000 SAMPLES..............II-38
11 MAINTENANCE ON AN AS-NEEDED BASIS..............................................................II-42
12 RESETTING THE SYSTEM WHEN SWITCHED TO THE STOP MODE
WHILE PERFORMING MAINTENANCE......................................................................II-64
When performing maintenance on the ISE unit be sure to wear protective gloves
to avoid infection and other injury and observe the “WARNING” and “CAUTION”
notations in this user guide.
CAUTION
The ISE maintenance flow, biweekly or longer periodic maintenance is managed on the basis
of either period of time or number of samples.
The AU480 ISE can process 200 samples per day.
II-1
ISE User Guide ISE MAINTENANCE FLOW
Version 1.0
1 ISE MAINTENANCE CHECKLIST

Photocopy the following maintenance checklist every month and use it for daily management of the
ISE unit. Check the appropriate box each day when ISE maintenance is performed.
Make a separate copy for each ISE unit.
Date (below)
Maintenance
Month: Year:
Daily 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31
5.1 Check the
Buffer Syringe for
Leaks
5.2 Auto-wash the
Sample Pot and
Electrode Line
Weekly 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31
6.1 Check the
Selectivity of
the Na and K
Electrodes
6.2 Enhanced
Cleaning of
Electrode Line
Every two
weeks or 3000 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31
Samples
7.1 Manually
Wash the Mix Bar
and Liquid Level
Sensors
7.2 Manually
Wash the Sample
Pot and the
Sample Pot Tubes
Every two
months or 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31
20000 Samples
8.1 Replace
the Na, K, or Cl
Electrode
8.2 Check
and Add REF
Electrode Solution
Every three
months or 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31
20000 Samples
9.1 Replace the
Roller Tubes for
MID Solution
Dispense and
Mixture Aspiration
9.2 Replace the
Sample Pot Tubes
9.3 Replace the
REF Block Side
Drain Tube and
the Pinch Valve
Tubing
9.4 Replace the
Drain Tube and
Manually Wash
the Drain Tank
II-2
ISE MAINTENANCE FLOW ISE User Guide
Version 1.0
Date (below)
Maintenance
Month: Year:
Every two
years or
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31
150000
Samples
10.1 Replace the
REF Electrode
and Packing
On an as
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31
needed basis
11.1 Replace the
Sample Pot
11.2 Clean the
Electrode Block
(Inlet Side)
11.3 Manually
Wash the K
Electrode
11.4 Manually
Wash and
Replace the REF
Electrode Block
11.5 Replace
Reagents
Replace Buffer
solution
Replace MID
solution
Replace REF
solution
11.6 Replace the
Buffer Syringe
11.7 Procedure
for Enhanced
Cleaning of the
Electrode Line
(Manual)
11.8 Replace the
Buffer Syringe
Case and Case
Head
II-3
Version 1.0
2 OVERVIEW OF ISE MAINTENANCE FLOW

To keep the system performing properly, perform maintenance in the following order.
Place a check on the “1. ISE MAINTENANCE CHECKLIST” after as each maintenance item is
completed.
DAILY MAINTENANCE
5.1 Check the Buffer Syringe for Leaks .......II-8

5.2 Auto-wash the Sample Pot and Electrode Line .......II-10
WEEKLY MAINTENANCE
6.1 Check the Selectivity of the Na and K Electrodes .......II-12

6.2 Enhanced Cleaning of Electrode Line .......II-14
MAINTENANCE REQUIRED EVERY TWO WEEKS OR 3000 SAMPLES
7.1 Manually Wash the Mix Bar and Liquid Level Sensors .......II-16
7.2 Manually Wash the Sample Pot and the Sample Pot Tubes .......II-18
MAINTENANCE REQUIRED EVERY TWO MONTHS OR 20,000 SAMPLES
8.1 Replace the Na, K, or Cl Electrode .......II-22

8.2 Check and Add REF Electrode Solution .......II-25
MAINTENANCE REQUIRED EVERY THREE MONTHS OR 20,000 SAMPLES
9.1 Replace the Roller Tubes for MID Solution Dispense

and Mixture Aspiration .......II-26
9.2 Replace the Sample Pot Tubes .......II-29
9.3 Replace the REF Block Side Drain Tube and the Pinch Valve Tubing.......II-32
9.4 Replace the Drain Tube and Manually Wash the Drain Tank .......II-35
MAINTENANCE REQUIRED EVERY TWO YEARS OR 150,000 SAMPLES
10.1 Replace the REF Electrode and Packing .......II-38
MAINTENANCE ON AN AS-NEEDED BASIS
11.1 Replace the Sample Pot .......II-42

11.2 Clean the Electrode Block (Inlet Side) .......II-44
11.3 Manually Wash the K Electrode .......II-47
11.4 Manually Wash and Replace the REF Electrode Block .......II-49
11.5 Replace Reagents .......II-53
11.6 Replace the Buffer Syringe .......II-55
11.7 Procedure for Enhanced Cleaning of the Electrode Line (Manual) .......II-58
11.8 Replace the Buffer Syringe Case and Case Head .......II-61
II-4
Version 1.0
3 LIST OF PARTS USED FOR ISE MAINTENANCE

The following table lists the names and numbers of the parts used for ISE maintenance. These parts
are described in detail in the maintenance procedures in chapter 5 and later. Use this parts list along
with “4. TUBING BLOCK DIAGRAM FOR ISE MAINTENANCE” as a reference for ordering parts.
Target
Maintenance
Maintenance Item Parts to Be Used Part Number Location
Interval
(in Diagram)
Daily 5.1 Check the Buffer - - (0)
Syringe for Leaks
5.2 Auto-wash the Sample ISE cleaning solution AUH1019 (1) to (11)
Pot and Electrode Line
Weekly 6.1 Check the Selectivity Selectivity check liquid AUH1018 Electrodes
of the Na and K
Electrodes
6.2 Enhanced Cleaning of ISE cleaning solution AUH1019 (1) to (11)
Electrode Line
Every two 7.1 Manually Wash the Ethyl alcohol (Ethanol) Commercial item (1) and (2)
weeks or 3,000 Mix Bar and Liquid Level
samples Sensors
7.2 Manually Wash the Wash solution OSR0001
Sample Pot and the
Sample Pot Tubes
Every two 8.1 Replace the Na, K, or Na electrode MU9194 Electrodes
months or Cl Electrode K electrode MU9195
20,000 samples
Cl electrode MU9196
O-ring MU9900
8.2 Check and Add REF REF electrode solution AUH1017
Electrode Solution
Every three 9.1 Replace the Roller Roller Tube MU9623 (9)
months or Tubes for MID Solution
20,000 samples Dispense and Mixture
Aspiration
9.2 Replace the Sample Tube set MU5386 (4)
Pot Tubes
9.3 Replace the REF Tube set 2 MU8247 (7) and (8)
Block Side Drain Tube Pinch valve tubes ZM2970
and the Pinch Valve
Tubing
9.4 Replace the Drain Drain Tube 1 MU8302 (10) and (11)
Tube and Manually Wash Sodium hypochlorite Commercial item
the Drain Tank solution
Every two years 10.1 Replace the REF REF Electrode (with the MU9197 Electrodes
or 150,000 Electrode and Packing packing)
samples The packing for REF MU9202
Electrode
II-5
Version 1.0
Target
Maintenance
Maintenance Item Parts to Be Used Part Number Location
Interval
(in Diagram)
On an as 11.1 Replace the Sample Sample Pot MU9627 (3)
needed basis Pot
11.2 Clean the Electrode Mandolin string(f0.3) ZM0227 (5)
Block (Inlet Side)
11.3 Manually Wash the K - - Electrodes
Electrode
11.4 Manually Wash REF electrode block 2 MU8245 (6)
and Replace the REF
Electrode Block
Wash solution OSR0001

11.5 Replace Reagents Buffer solution AUH1011
MID solution AUH1012
REF solution AUH1013
11.6 Replace the Buffer R Syringe ZM0112 (0)
Syringe
11.7 Procedure for ISE cleaning solution AUH1019 (1) to (11)
Enhanced Cleaning of the Pipette Commercial item
Electrode Line (Manual)
11.8 Replace the Buffer ISE syringe case ZM1362
Syringe Case and Case
Head
II-6
Version 1.0
4
Version 1.0
Level sensor connector
Mixer connector
ISE User Guide

(1) Liquid level sensor
←
Nozzle ←
MID solution
(2) Mix bar (3) Sample pot
Buffer solution
Roller pump
(for MID solution dispense)
(4) Tubes between the (0)
sample pot and

MAINTENANCE
electrode unit (tube set) (9) Roller tube

T-connector
Buffer syringe
B
E
Description of Tubing Block Diagram Symbols
(5) Electrode block CI (0) to (11): The numbers of tubing, etc.,
(8) Pinch valve tubing
(inlet) for which maintenance is performed
A to H: The symbols of detaching locations, etc.,
Na Electrode that are shown in the detail description
F of maintenance routine
K
REF solution tube

REF C
G
TUBING BLOCK DIAGRAM FOR ISE
D
→Waste solution
(6) REF
electrode block H
(10) Drain tube
↑ (7) REF block-side

drain tube (tube set) Roller pump (11) Drain tank
ISE MAINTENANCE FLOW

(for mixture
REF solution aspiration)
A
(9) Roller tube
II-7
5 DAILY MAINTENANCE
5.1 Check the Buffer Syringe for Leaks

The buffer syringe dispenses a precise small amount of serum or reagent. If liquid leaks from
the syringe, the correct amount of buffer solution is not dispensed and may cause the syringe to
malfunction. Before starting daily analysis, be sure to check the buffer solution and sample syringes
for leaks.
Maintenance location: No. 0 in the ISE tubing block diagram
Fixing screws
Case head
(0)
Syringe case Syringe
Buffer syringe
Procedure for check the Buffer Syringe for Leaks

Materials:
Dry, clean cloth or paper towel
The R syringe is used for the ISE buffer solution.

If the syringe needs to be replaced because of leaks check, prepare a new R
TIP syringe.
1. Open the right front door of the analyzer.

2. Verify that the system in either Warm up or Standby mode and the power to the system is off.
• If protective gloves, etc., get wet, thoroughly rinse them immediately with water.
• Verify that strong alkali such as AU wash solution alkali does not come in contact
CAUTION with the syringe cases. If the alkali wash solution comes in contact with the
syringe case cracks may occur. If there is contact with the syringe case rinse
thoroughly with water.
3. Using a dry cloth, wipe the bottom of the syringe case, the joint of the case head and syringe
case, and the vicinity of fixing screws to check for leaks.
If any of the liquid has leaked, wipe it off with a dry clean cloth.
4. Visually check the case head for cracks.
If any cracks are found on the case head, replace it with a new one. After replacing the case head
advance to step 9.
5. Check to see if the case head and the syringe are loose.
• If the case head and syringe case are loose, tighten them by turning the syringe case
clockwise toward the case head.
• If any leak was found, allow approximately five minutes after tightening the case head and
syringe case. Then, recheck the bottom of the syringe case for leaks.
II-8
Version 1.0
6. Check to see if the two fixing screws on the case head, fixing nut, and piston fixing screw are
loose.
• If loosen, firmly tighten the fixing screws, fixing nut, and piston fixing screw.
• If a leak was found, allow approximately five minutes after tightening the case head and
syringe case. Then, recheck the bottom of the syringe case, etc. for leaks.
7. Visually check to see if any liquid is leaking inside the syringe case.
If any leak was found, go to section “11.6 Replace the Buffer Syringe” in this chapter.
8. From the AU480 “Home” window select Menu List>Maintenance>User Maintenance>ISE
Maintenance>Maintenance to display the “ISE Maintenance: Maintenance tab” window.
9. Select I n s p e c t I S E b u f f e r S y r i n g e f o r L e a k from the list of maintenance

items.
10. Select U p d a t e .
A confirmation window appears.
11. Select O K .
12. Close the right front door of the analyzer.
• Reagent may drip from the case head during syringe replacement. Wipe off the
reagent with a paper towel.
CAUTION • If leaks still occur even after tightening the loose parts, replace the syringe.
II-9
Version 1.0
5.2 Auto-wash the Sample Pot and Electrode Line
When handling wash solution, wear protective gloves to avoid contact with the wash
solution.
If hands or clothing come into contact with the wash solution, wash them
CAUTION immediately with water. Should wash solution get splashed accidentally in the eyes
or mouth, rinse it immediately with water and consult a physician.
If the sample pot and electrode line remain unwashed, contamination between samples will occur,
which may result in a calibration or data error. After completing daily analysis, be sure to auto-wash
the sample pot and electrode line. It takes approximately 4 minutes from start to auto-wash the sample
pot and electrode line.
If performing an analysis after auto-wash be sure to execute calibration before starting the analysis.
Procedure for Auto-wash the Sample Pot and Electrode Line

Materials:
• ISE Cleaning Solution : AUH1019
• Sample cup : MU8532
1. Verify that the system is in Warm up or Standby mode.

2. Fill the sample cup with approximately 1 mL of ISE Cleaning Solution.
3. Open the sub cover of the STAT table and press the TA B L E R OTAT I O N / D I A G button.
Rotate the STAT table until the “CLEAN” position marked on the STAT table is visible.
4. Set the sample cup filled with the ISE Cleaning Solution in the “CLEAN” position on the STAT
table.
Avoid spilling wash solution when handling the reagent bottle. If the wash solution is
spilled, wipe it off immediately with a cloth or paper towel soaked in water.
CAUTION
II-10
Version 1.0

6. Select A u t o - w a s h i n g S a m p l e Po t & E l e c t r o d e L i n e from the list of

maintenance items.
The “Single Operation” buttons appear to the right of the Maintenance items list.
7. Select C l e a n i n g ( F 5 ) .
8. Select O K .
Maintenance date is updated automatically.
9. After completing an auto-wash operation, remove the sample cup filled with the wash solution out
of the system.
When starting analysis again, be sure to calibrate before analysis.

CAUTION
II-11
Version 1.0
6 WEEKLY MAINTENANCE
6.1 Check the Selectivity of the Na and K Electrodes

The Na electrode and K electrode are ion-selective electrodes. If the selectivity of electrodes
deteriorates, the ISE unit is affected by ions other than those to be measured, affecting analysis
results.
To check the electrodes for deterioration, check the selectivity of the Na and K electrodes every week
after terminating analysis.
Do not leave the bottle of selectivity check liquid open. Evaporation may effect the
concentration of the selectivity check liquid or it may crystallize.
CAUTION
Procedure for Check the Selectivity

Materials:
• Selectivity check liquid : AUH1018
1. Verify that the system is in the Warm up or Standby mode.

2. Pour the Na and K electrode check liquid in separate sample cups.
3. Open the STAT table cover (small).
4. Press the TA B L E R OTAT I O N / D I A G button. Rotate the STAT table until the U-H and
U-L positions are visible on the STAT table.
5. Place the filled sample cups on the STAT table.
• Place the Na-electrode sample cup in the U-H (SEL-Na) position.
• Place the K-electrode sample cup in the U-L (SEL-K) position.

6. Close the STAT table cover (small).
II-12
Version 1.0

8. Select the S e l e c t i v i t y C h e c k tab to display the “ISE Maintenance: Selectivity Check

tab” window.
9. Select C h e c k S t a r t .
The “Selectivity Check” window appears.
10. Select O K .
The selectivity check procedure will start and display the check results.
11. Check the selectivity data.
For abnormal data, the background of the numeric value is displayed in yellow.
The system will judge a value more than 160 mmol/L for Na-electrode and a value more than 6.0
mmol/L for K-electrode as abnormal data.
If the check results are abnormal, replace the relevant electrode.
For details on replacing the electrode, refer to section “8.1 Replace the Na, K, or Cl Electrode” in
this chapter.
II-13
Version 1.0
12. Remove previously placed Na and K sample cups from STAT table.
13. The maintenance date will be automatically updated in the list.
6.2 Enhanced Cleaning of Electrode Line

If the sample pot and electrode line remain unwashed, contamination between samples will occur,
resulting in a calibration or data error. After the Na and K selectivity check, perform the enhanced
cleaning of the electrode line procedure.
Refer to Nos. (1) to (11) in the “4 TUBING BLOCK DIAGRAM FOR ISE MAINTENANCE” in this
chapter.
Procedure for Enhanced Cleaning of the Electrode Line (Auto)

Materials:
solution.

2. Fill the sample cup with 1.5 mL of ISE Cleaning Solution.
3. Set the sample cup filled with the wash solution in the “CLEAN” position on the STAT table.
5. Select C l e a n i n g ( E n h a n c e d ) from the list of maintenance items.

The “Start” window appears.
6. Select C l e a n i n g ( E n h a n c e d ) ( F 6 ) .
II-14
Version 1.0
7. Select O K .
The enhanced cleaning operation starts. This procedure requires approximately 30 minutes to
complete.
Maintenance date is automatically updated in the list.
8. After completing enhanced cleaning operation, remove the sample cup filled with the wash
solution from the STAT table.
The enhanced cleaning of the electrode line can be run in a W2 operation.

TIP
II-15
Version 1.0
7 MAINTENANCE REQUIRED EVERY TWO

WEEKS OR 3,000 SAMPLES
7.1 Manually Wash the Mix Bar and Liquid Level Sensors
If the mix bar and liquid level sensors are not washed over an extended period of time, they become
contaminated with serum, urine or a fur, etc.
Wash the mix bar and liquid level sensors with alcohol every two weeks or every 3,000 samples,
whichever comes first.
Maintenance locations: Nos. (1) and (2) in the ISE tubing block diagram
Mixing unit Mixing motor connector
Mixing unit clamp knob
Buffer solution and
MID solution connecting tubes
(1) Liquid level sensor
Nozzle
(2) Mix bar

(3) Sample pot
Procedure for Wash the Mix Bar and Liquid Level Sensors
Materials:
• Ethyl alcohol (Ethanol)
• Dry, clean cloth or paper towel

2. Open the main cover.
3. Open the ISE cover.
II-16
Version 1.0

5. Select Wa s h i n g L i q u i d L e v e l S e n s o r s ( M i x b a r ) from the list of

maintenance items.
6. Select D ra i n - F l o w c e l l from the “Single Operation” buttons.
7. Select O K .
8. Press the TA B L E R OTAT I O N / D I A G button once.
The buffer solution in the sample pot is drained.
9. Disconnect the connectors of the liquid level sensor (714) and mixing motor (706) from the mixing
unit.
10. Loosen the knob clamping the mixing unit. Gently lift the mixing unit to dismount it.
Be careful not to bend or break the liquid level sensors when washing them.
CAUTION
11. Wipe the two liquid level sensors and mix bar with a cloth dampened with ethyl alcohol (ethanol).
Mix bar
Nozzle
Connecting tubes
Liquid level sensors
Mixing unit
12. Gently remount the mixing unit in its original position.

13. Tighten the knob to secure the mixing unit.
II-17
Version 1.0
14. Reconnect the connectors of the liquid level sensor (714) and mixing motor (706) to the mixing
unit.
15. Press the TA B L E R OTAT I O N / D I A G button.
The MID solution is supplied.
16. Close the ISE cover.
17. Close the main cover.
Do not change the orientation and position of two nozzles attached to the mixing unit
and do not use force pulling on the connecting tubes of the mixing unit.
CAUTION
19. Select O K .
7.2 Manually Wash the Sample Pot and the Sample Pot Tubes
The sample pot tubes are the three tubes that run between the sample pot, electrode unit, and T-
connector. When the system processes samples that contain large amounts of fiblin and protein debris
may build up in the tubes over time.
(3) Sample pot
(4)Tubes between
the sample pot and
electrode unit (tube set)
T-connector
Electrode unit
.
II-18
Version 1.0
Procedure for Wash the Sample Pot, Tubes between the Sample Pot and
Electrode Unit, and T-connector
Materials:
• Wash solution: OSR0001
• Deionized water or distilled water
• Dry, clean cloth or paper towel
• Sonicator filled with deionized water

3. Select C l e a n s a m p l e p o t , t u b e s , & T- c o n n e c t o r from the list of maintenance

items.
5. Select O K .
unit.
8. Loosen the knob clamping the mixing unit. Gently lift the mixing unit to dismount it and place it on
mount plate.
9. Loosen the sample pot clamp knob and lift the sample pot.
10. Loosen the tube connected to the bottom of the sample pot, and then dismount the sample pot.
Remove the sample pot and tubes on the electrode unit side (location (4) in the tubing block
diagram) from the electrode unit.
II-19
Version 1.0
11. Remove the tube attached to the bottom of the sample pot.
12. Remove the tube from the electrode unit and the bypass tube.
13. Remove three tubes from the T-connector.
14. Supply washing water or the sample diluent of approximately 2% concentration of alkali wash
solution to the sonicator filled with deionized water.
To prevent the REF electrode block from deforming due to the ultrasonic cleaning,
follow these precautions.
• Do not perform ultrasonic cleaning for more than ten minutes.
CAUTION
• Use cleaning liquid at room temperature.
• Use the sonicator rated at 600W or less.

If the output of the sonicator is uncertain, contact the manufacturer of the
sonicator.
15. Wash the T-connector and the two tubes together with the sample pot in the sonicator filled with
deionized water for approximately 10 minutes.
16. Rinse the sample pot, T-connector, and the three tubes with deionized water or distilled water.
17. Wipe the sample pot, T-connector, and the three tubes with a wiper cloth.
18. Connect the three tubes to the T-connector.
Make sure to push the tubes all the way on the T connector to avoid leaks.
CAUTION
19. Connect the other end of the tube to the original position of the electrode unit and the bypass
tube.
20. Attach the tube to the bottom of the sample pot.
Tighten the tube knob firmly.
Tighten the knob firmly when attaching the tube to the bottom of the sample pot.
CAUTION
21. Remount the sample pot in place.

Insert the sample pot fixing knob into the notch on a side of the sample pot, and then match the
hole on the other side of the pot with the positioning pin.
22. Tighten the sample pot fixing knob to secure the sample pot.
23. Gently remount the mixing unit in place, then secure the unit by tightening the knob.
When remounting the mixing unit be careful not to get the tube caught in the
analyzer’s peripheral part.
CAUTION
II-20
Version 1.0
24. Reconnect the connectors of the mixing unit and the mixing motor.
25. Press the TA B L E R OTAT I O N / D I A G two or three times.
The MID solution is supplied.
Confirm that liquid is properly aspired from the sample pot to the electrode unit. If liquid remains
in the sample pot, check the tubes connections.
29. Select O K .
II-21
Version 1.0
8 MAINTENANCE REQUIRED EVERY TWO

MONTHS OR 20,000 SAMPLES
8.1 Replace the Na, K, or Cl Electrode

If the electrodes have deteriorated, analysis results will be effected. Replace the electrodes every two
months or every 20,000 samples, whichever comes first. If a calibration measurement value fluctuates
markedly higher or lower than a previous day, or if an alarm message appears on the window, replace
the corresponding electrode.
Maintenance location: Electrodes in the ISE tubing block diagram

Sample pot
E (O-ring)
CI Cl electrode cord (blue)
Electrodes Na Na electrode cord (yellow)
K K electrode cord (red)
REF
Lock lever
Replace the Na, K, or Cl Electrode

Materials:
• Na electrode : MU9194
• K electrode : MU9195
• Cl electrode : MU9196
• O-ring : MU9900
1. Verify that the system has entered Warm up or Standby mode.

II-22
Version 1.0

5. Select Re p l a c i n g N a , K , o r C l E l e c t r o d e from the list of maintenance items.

7. Select O K .
8. Press the TA B L E R OTAT I O N / D I A G button once.
The liquid remaining in the electrodes is drained.
Select D ra i n - F l o w c e l l and then press the TA B L E R OTAT I O N /

D I A G button. The first time the button is pressed, liquid is drained from the tube.
TIP Each successive time the button is pressed, MID solution is injected into the tube.
9. Move the lock lever to the back, if viewed from the front, then unlock the electrodes
to be replaced.
10. Remove the three electrodes.
11. Disconnect the cords from the Cl electrode, Na electrode, or K electrode.
Three O-rings are attached to one side of three new electrodes. Be careful not to
lose the O-rings when mounting the electrodes.
An O-ring is not attached to the right side of the Cl electrode. Use the separately
CAUTION packaged O-ring to insert between the right side of Cl electrode and the metal plate.
II-23
Version 1.0
12. Connect the blue cord, yellow cord, and red cord to the Cl electrode, Na electrode, and K
electrode, respectively.
13. Mount the three new electrodes on the electrode block.
Mount the electrodes in order of Cl, Na, and K from the sample pot side to the front side.
14. Move the lock lever forward to clamp the electrodes.
The MID solution is supplied to the tube.
The two roller pumps turn on and move the solution in each roller tube.
Press and hold down the TA B L E R OTAT I O N / D I A G button until the roller pumps are
activated five times. Visually check the tube to see if the solution passing through the electrodes
includes air bubbles. Repeat this operation until there are no air bubbles in the tube.
17. Enter the serial number of each replacement electrode, and then select O K .
The “Electrode Serial No. Setup” window is closed.
20. Allows at least 5 minutes after closing the main cover, and then perform calibration analysis.
To obtain the best possible analysis data, perform two successive calibration
measurements to confirm the electrode stability.
If the difference in factor value between the first and second calibrations is within the
CAUTION following values, the electrodes are stable. The MID solution concentrations in the
following table have been set from “Specific Test Parameters: ISE” menus.
Na K CI
MID solution concentration 140 4.0 100
Difference between 1st and 2nd factors 0.020 0.045 0.025
• If the difference between those factor values is not within each value shown
above, of if slope 0 is output at the first calibration:
There is a possibility that air remains inside the electrode block. First, open the
main cover and ISE cover.
From the AU480 “Home” window select Menu List>Maintenance>User
Maintenance>ISE Maintenance>Maintenance to display the “ISE
Maintenance: Maintenance tab” window.
Select the “ISE Maintenance” check box. After selecting the M I D / R E F
P r i m e , press the TA B L E R OTAT I O N / D I A G button.
• If slope 0 is output in both calibrations:

The electrodes might not be set correctly. Remove the electrodes once, then
repeat steps 9 to 15 in the above maintenance procedure.
II-24
Version 1.0
8.2 Check and Add REF Electrode Solution

Visually check the REF electrode and add the REF electrode solution if the solution falls below the
reference line.
REF electrode
Reference line
Add the REF electrode solution

Materials:
REF electrode solution: AUH1017
1. Remove the cap on the REF electrode, then add the REF electrode solution until it is above the
reference line.
If air bubbles are visible in the electrode of the REF electrode block, turn the
electrode downward and tap it with finger. Refer to section “10.1 Replace the REF
CAUTION Electrode and Packing” in this chapter.

3. Select C h e c k & s u p p l y l i q u i d o f R E F E l e c t r o d e from the list of

Maintenance items.
5. Select O K .
II-25
Version 1.0
9 MAINTENANCE REQUIRED EVERY THREE

MONTHS OR 20,000 SAMPLES
When performing maintenance required every three months or every 20,000 samples, replace
the roller tubes for MID solution dispense, mixture aspiration, and individual tubes (tubes between
the sample pot and electrode unit, drain tube on the REF electrode block side, pinch valve tubing,
and drain tube). This section describes a series of procedure in which maintenance is performed
concurrently for all tubes.
When performing maintenance for each tube separately, observe the procedure of a relevant
maintenance item described in section 9.1 or later.
9.1 Replace the Roller Tubes for MID Solution Dispense and
Mixture Aspiration
The roller tubes will deteriorate due to friction with each roller pump and vibrations. If used for an
extended period of time these tubes will break. Replace the roller tubes with new ones every three
months or every 20,000 samples.
Maintenance locations: No. (9) in the ISE tubing block diagram

Tube connectors
Tube number Tube number
Pump-side tube number Pump-side tube number
Roller pump
(9) Roller tube
II-26
Version 1.0
Procedure for Replace Roller Tubes

Materials:
Roller tube: MU9623

3. Select Re p l a c i n g M i x t u r e & P u m p Ro l l e r Tu b i n g from the list of

maintenance items.
6. Remove the MID solution roller tube from the roller pump and the mixture roller tube from the
other roller pump.
• Avoid spilling solution from the roller tube when replacing the tube.
• The roller tube consists of the MID solution roller tube and waste liquid roller
CAUTION tube. Replace these two roller tubes at the same time.
7. Turn each roller tube connector to remove the roller tube.

8. Connect a new roller tube.
Turn the connectors at both ends of the tube to secure it.
II-27
Version 1.0
9. Connect the roller tube with the roller pump, matching connectors with the notches on the roller
pump.
Connect the MID solution roller tube and waste liquid roller tube with each roller pump in the
same manner as the roller tube pump.
Make the connection so the numbers on the tubes match those on the notches near the roller
pump.
If connected incorrectly, liquid will not be supplied or drained correctly.

CAUTION
10. Select P r i m e B y p a s s button.

11. Select O K .
The two roller pumps are activated and solution flows into the individual roller tubes.
The roller pumps rotate for approximately one minute to bleed the air from the tubes.
13. Ensure that the roller tubes are filled with the MID solution.
15. Select O K .
II-28
Version 1.0
9.2 Replace the Sample Pot Tubes

If the system analyzes samples that contain large amounts of fibrin and protein such as a dialysis
sample, fibrin, protein, etc. will accumulate near the T-connector between the sample pot and
electrode unit, which may result in a calibration or data error.
Since the accumulated contaminants may not be removed with the washing procedure performed
every two weeks or every 3000 samples, replace the tubes and T-connector with new ones every
three months or 20,000 samples.
For details on how to clean sample pot, tubes, and T-connector, refer to section “7.2 Manually Wash
the Sample Pot and the Sample Pot Tubes” in this chapter.
Maintenance location: No. (4) in the ISE tubing block diagram
(3) Sample pot
(4)Tubes between
the sample pot and
T-connector
Electrode unit
Replace the Tubes between Sample Pot and Electrode Unit, and T-connector
Materials:
Tube set: MU5386
Getting the System Ready for Maintenance
Prepare the system for maintenance before beginning the procedure. If the operation
is not performed in Warm up or Standby, liquid may be dispensed from nozzles,
CAUTION tubes, etc. during maintenance.

II-29
Version 1.0

5. Select Re p l a c e s a m p l e Po t , t u b e s , & T- c o n n e c t o r from the list of

maintenance items.
7. Select O K .

TIP Each successive time the button is pressed, the MID solution is injected into the
tube.
II-30
Version 1.0
Performing the Maintenance Procedure

unit.
mount plate.
3. Loosen the sample pot clamp knob and lift the sample pot.
4. Loosen the tube connected to the bottom of the sample pot, and then dismount the sample pot.
Remove the sample pot and tubes on the electrode unit side (location (4) in the tubing block
diagram) from the electrode unit.
5. Connect the new set of tubes in place in order of the electrode unit side and waste liquid side (B
in the tubing block diagram).
Attach the tube to the bottom of the sample pot.
Make sure to push the tubes all the way on the T connector to avoid leaks. Tighten
the knob firmly when attaching the tube to the bottom of the sample pot.
CAUTION

Insert the sample pot clamp knob into the notch on a side of the sample pot, and then match the
7. Tighten the sample pot clamp knob to clamp the sample pot.
8. Gently remount the mixing unit in place, and then clamp the unit by tightening the knob.
When remounting the mixing unit in place, be careful not to splash the buffer solution
and MID solution.
CAUTION
9. Reconnect the connectors of the liquid level sensor (714) and mixing motor (706) of the mixing
unit in place.
10. Confirm that D ra i n - F l o w c e l l is selected on the “ISE Maintenance: Maintenance tab”
window.
11. Press the TA B L E R OTAT I O N / D I A G button two or three times.
Ensure that liquid is properly aspired from the sample pot to the electrode unit.
If liquid remains in the sample pot, check the tube connections.
13. Select O K .
Refer to “ (1) Troubleshooting for ISE Sample Pot Liquid level Sensor Error -1 (3186, 3187)” in section
“3. TROUBLESHOOTING” in Part I.
II-31
Version 1.0
9.3 Replace the REF Block Side Drain Tube and the Pinch Valve
Tubing
The REF block-side drain tube is subject to accumulation of contaminants and may adversely affect
analysis data due to flow-rate decrease and noise interference if it is used over an extended period of
time. Replace the drain tube with a new one every three months or every 20,000 samples.
The pinch valve tubing is provided with pinch valves.
If the pinch valve tubing is used for an extended period of time, the section pinched by the valve
will deteriorate. Replace the pinch valve tubing with a new one every three months or every 20,000
samples. Concurrently replace the REF block-side drain tube and pinch valve tubing.
B
Tubes between
the sample pot and
CI (8) Pinch valve tube
Na
Electrodes
K
REF C
(6)REF electrode
block
(7)REF block-side
drain tube Pinch valve
(tube set 2)
A
Procedure for Replace the REF Block Side Drain Tube and Pinch Valve Tubing
Materials:
• Tube set 2 : MU8247
• Pinch valve tubing : ZM2970
II-32
Version 1.0
Prepare the system for maintenance before beginning the procedure. If the operation
is not performed in Warm up or Standby, liquid may be dispensed from nozzles,
CAUTION tubes, etc. during maintenance.

5. Select Re p l a c i n g D ra i n t u b e s & Va l v e t u b e s from the list of maintenance

items.
7. Select O K .

II-33
Version 1.0

1. Move the lock lever toward the back to release the electrodes from being clamped.
2. Gently lift up the block on which the REF electrode is mounted.
3. Pull out the REF electrode cord.
4. While holding the REF electrode block by hand, pull the tube set 2 out of the REF electrode
block.
5. Attach the tube side of a new tube set 2 to the REF electrode block (6).
6. Reconnect the REF electrode cord in place.
7. Set the REF electrode block in the original position.
8. Move the lock lever to the clamp position.
9. Disconnect the three ends of the pinch valve tubing.
Disconnect the tube ends at positions A, B and C in the tubing block diagram.
10. Remove the pinch valve tubing from the pinch valve groove.
Remove the tube in the top groove of the pinch valve directly upward. Remove the tube in the
bottom groove of the pinch valve upward while pressing the top end of the pinch valve with finger.
Do not mount each branch tube in a different groove on the pinch valve. If a tube is
mounted backwards, liquid will not be supplied and drained correctly.
Mount a shorter tube in the bottom groove of the pinch valve (between A and C in
CAUTION the tubing block diagram). Mount longer tube in the top groove of the pinch valve
(between B and C in the tubing block diagram).
11. Mount a new section of pinch valve tubing by connecting the branches at positions A, B, and C in
the tubing block diagram.
Connect the longer Y-branched tube at position B.
12. Insert the two branch tubes into the grooves on the pinch valve, so that each tube passes through
the middle of the groove.
Mount the tube in the bottom groove of the pinch valve while pressing the top end of the pinch
valve with finger, in the same way the tube was removed. Mount the tube in the top groove of the
pinch valve directly.
To position B
Groove
To position A
Pinch valve
Pass the tube through

the middle of the groove
13. Confirm that D ra i n - F l o w c e l l is selected on the “ISE Maintenance: Maintenance tab”

window.
II-34
Version 1.0

Press and hold the TA B L E R OTAT I O N / D I A G button until the roller pumps are
activated five times. Verify that there are no air bubbles in the tubes.
Hold down the TA B L E R OTAT I O N / D I A G button until no air bubbles are found in the
tube.
Confirm that liquid is properly aspired from the sample pot to the electrode unit.
If liquid remains in the sample pot, check the tubes connections.
16. Select O K .
Refer to “ (1) Troubleshooting for ISE Sample Pot Liquid level Sensor Error -1 (3186, 3187)” in section
“3. TROUBLESHOOTING” in Part I.
9.4 Replace the Drain Tube and Manually Wash the Drain Tank
If the system uses samples that contain large amounts of fibrin and protein such as a dialysis sample,
fibrin, protein, etc. debris will accumulate at the drain tube outlet and drain tank, resulting in a
calibration or data error.
Replace the drain tube and manually wash the drain tank every three months or 20,000 samples,
whichever comes first.
D
Pinch valve tubing → Waste solution H
(10) Drain tube
Roller pump
(for mixture aspiration)
G (Joint No.3)
(11) Drain tank
Procedure for Replace the Drain Tube and Manually Wash the Drain Tank
Materials:
• Drain Tube 1 : MU8302
• Sodium hypochlorite solution commercially available

*Prepare the solution with deionized water so that the available chlorine concentration becomes
approximately 0.5% or more.
(To prepare a sodium hypochlorite solution prescribed as more than 5% of available chlorine,
add 90 mL of deionized water to 10 mL of sodium hypochlorite.)
II-35
Version 1.0

5. Select Re p l a c e D ra i n Tu b i n g & c l e a n d ra i n w e l l from the list of

maintenance items.
7. Select O K .
The liquid in the waste bypass tube is drained into the drain tank.
II-36
Version 1.0

1. Remove the drain tube end from the tube joint (No.3) of the roller pump for mixture aspiration.
The drain tube end on the roller pump side in block G in the tubing block diagram is
disconnected.
2. Remove the drain tube end from the hook on the drain tank, and then dismount the whole drain
tube.
The drain tube in block D in the tubing block diagram is dismounted.
When handling the solution hypochlorite solution, wear protective gloves to avoid
coming into contact with the sodium hypochlorite solution.
3. Prepare approximately 50 mL of the diluted sodium hypochlorite solution.

4. Pour the diluted sodium hypochlorite solution into the drain tank directly from its top (position H in
the tubing block diagram).
Pour 50 mL as a guide of the solution amount.
5. Allow approximately 10 minutes after pouring the sodium hypochlorite solution, and then pour a
sufficient amount of water into the drain tank from its top (position H in the tubing block diagram)
to rinse out the sodium hypochlorite solution.
6. Connect a new drain tube to the mixture aspiration roller tube and the drain tank side, and then
remount it in place.
When connecting the tubing, make sure to push the tube all the way to avoid leaking
joints.
CAUTION
Verify that the solution aspired reliably from the sample pot into the electrode unit
and drained from the drain tube, and verify that no air bubbles are present in the
drain tank.
CAUTION If solution remains in the sample pot, make sure the tubes are connected correctly.
9. Select O K .
II-37
Version 1.0
10 MAINTENANCE REQUIRED EVERY TWO YEARS

OR 150,000 SAMPLES
10.1 Replace the REF Electrode and Packing

Replace the REF electrode and packing every two years or every 150,000 samples, whichever comes
first.
If all calibration measurement values of Na, K, and Cl fluctuate markedly higher or lower than
previously, or if an alarm message appears on the window, replace all the electrodes.
Maintenance location: Electrodes in the ISE tubing block diag
CI
Na
REF electrode packing
K Cap screw
REF solution REF REF electrode cord

(green)
Electrode unit
Replace the REF electrode and Packing

Materials:
• REF electrode (with the packing) : MU9197
• REF electrode packing : MU9202
To avoid damage when replacing the REF electrode to not use force mounting or
dismounting the electrode. When dismounting or mounting the electrode, be careful
CAUTION not to break the electrode unit.
II-38
Version 1.0

5. Select Re p l a c i n g R E F E l e c t r o d e a n d p a c k i n g from the list of maintenance

items.
7. Select O K .
Liquid that remains in the area of electrodes will be drained.
9. Travel the lock lever back to release the electrode unit from being locked.
10. Gently lift the block on which the REF electrode is mounted.
11. Pull out the REF electrode cord.
When loosening the set screw, do not press the connector of the thermistor cord by
hand, otherwise the cord could break.
CAUTION
12. Loosen the set screw, then gently remove the REF electrode along with the set screw.
13. Remove the packing of the REF electrode.
II-39
Version 1.0
Attaching the REF electrode

1. Verify that no air bubbles are in the electrode bar on a new REF electrode.
If air bubbles are found on the electrode bar of the new REF electrode, remove the bubbles by
pointing the electrode bar down and tapping it with a finger.
2. Insert a new packing into the REF electrode insertion hole.
At this time verify that the packing is not cracked or broken. If so, replace the packing.
3. Insert the set screw into the REF electrode insertion hole while turning it.
Insert the screw all the way until torque changes.
4. Insert the REF electrode into the REF electrode insertion hole (through the set screw).
Insert the REF electrode so that the electrode tip can be properly inserted into the packing.
The REF electrode tip can be easily inserted into the packing if it is dampened with
deionized water.
TIP
5. After inserting the REF electrode tighten the coupling screw by rotating it 1/4 to 1/2 turn, then
secure the REF electrode.
6. Connect the REF electrode cord correctly.
7. Remount the block in place.
8. Clamp the lock lever.
9. Select M I D / R E F P r i m e .
10. Select O K .
The MID solution will be injected into the tube.
Press the TA B L E R OTAT I O N / D I A G button until the roller pumps are activated five
times or so. Visually check for air bubbles in the tubes.
Press the TA B L E R OTAT I O N / D I A G button again until no air bubbles are found in the
tube.
If air bubbles will not purge, push in the cap screw.
The “Electrode Serial No. Setup” window appears.
13. Enter the serial number of the new reference electrode, and then select O K .
II-40
Version 1.0
16. Allows at least 5 minutes after closing the main cover, and then perform calibration analysis.
CAUTION following values, the electrodes are stable. The MID solution concentrations in the
following table are those set up in “Specific Test Parameters: ISE” menus.
Na K CI
MID solution concentration 140 4.0 100
Difference between 1st and 2nd factors 0.020 0.045 0.025
above, or if slope 0 is output at the first calibration:
There is a possibility that air remains inside the electrode block. First, open the
main cover and ISE cover.
From the AU480 “Home” window select Menu List>Maintenance>User
Maintenance>ISE Maintenance>Maintenance to display the “ISE
Maintenance: Maintenance tab” window.
After selecting the “ISE Maintenance” check box, select M I D / R E F
P r i m e , and then repeat steps 9 to 12 in the above maintenance procedure.
• If slope 0 is output in both calibrations:

The electrodes might not be set correctly. Remove the electrodes once, then
repeat steps 1 to 16 in the above maintenance procedure.
II-41
Version 1.0
11 MAINTENANCE ON AN AS-NEEDED BASIS
11.1 Replace the Sample Pot

Replace the sample pot if accumulated contaminants cannot be removed during the washing
procedure performed every two weeks or every 3000 samples or if any breakage or a large flaw is
found in the pot.

Mixing unit Mixing motor connector
Mixing unit clamp knob

Buffer solution and
(1) Liquid level sensor MID solution connecting tubes
Nozzles
(2) Mix bar

(3) Sample pot
Procedure for Replace the Sample Pot

Materials:
Sample pot: MU9627

II-42
Version 1.0
5. Select Re p l a c i n g S a m p l e Po t from the list of maintenance items.


D I A G button. The first time this button is pressed, liquid is drained from the tube.

unit.
mount plate.
3. Loosen the sample pot clamp knob to dismount the sample pot.
4. Loosen the knob on the tube attached to the bottom of the sample pot to remove the tube from
the pot.
5. Attach the tube to the bottom of a new sample pot.
Tighten the knob firmly when attaching the tube to the bottom of the sample pot.
TIP

7. Insert the sample pot clamp knob into the notch on a side of the sample pot, and then match the
8. Tighten the sample pot clamp knob to clamp the sample pot.
9. Gently remount the mixing unit in place, and then clamp the unit by tightening the knob.
When remounting the mixing unit in place, avoid splashing the buffer solution and
MID solution.
CAUTION
10. Reconnect the connectors of the liquid level sensor (714) and mixing motor (706) of the mixing
unit in place.
11. Press the TA B L E R OTAT I O N / D I A G button two or three times.
Confirm that liquid is properly aspired from the sample pot to the electrode unit.
If liquid remains in the sample pot check, the tube connections.
13. Select O K .
II-43
Version 1.0

Refer to “ (1) Troubleshooting for ISE Sample Pot Liquid level Sensor Error -1 (3186, 3187)” and “ (4)
Troubleshooting for Concurrent Reproducibility Error” in section “3. TROUBLESHOOTING” in Part I.
11.2 Clean the Electrode Block (Inlet Side)

When performing maintenance of the REF electrode block, check the block on the sample pot side
because contaminants may have accumulated there. Perform maintenance of the electrode block on the
sample pot side.

Mandolin string
(5) Electrode block CI Cl electrode cord (blue)

(inlet side)
Na Na electrode cord (yellow)
K K electrode cord (orange)
Electrodes
REF
Clean the Electrode Block (Inlet Side)

Materials:
Mandolin: ZM0227
II-44
Version 1.0

5. Select C l e a n i n g B l o c k o f E l e c t r o d e from the list of maintenance items.

7. Select O K .

TIP Each time the button pressed in succession, MID solution is injected into the tube.
II-45
Version 1.0

1. Move the lock lever to release the electrodes from being clamped.
2. Remove the three electrodes from the electrode unit.
3. Pull the cords connected to the Cl electrode, Na electrode, and K electrode out of each electrode.
4. Remove the tube connecting to the sample pot from the electrode block.
Four O-rings are attached to each side of three electrodes and the metal part
(location E in the tubing block diagram) that comes in contact with the Cl electrode.
CAUTION Be careful not to loosen the O-rings when removing the electrodes.
5. Pass the mandolin strings through the flow path hole on the side of the electrode block.
If contamination disturbed, it may get stuck in the mixture flow path in the electrode block. Bind
the mandolin strings up to the maximum thickness that can pass through the flow path.
6. Remove the stuck contamination by turning the mandolin strings while holding both ends by
hand.
When contaminants stick on the mandolin strings, wipe them with a dry, clean cloth or paper
towel several times.
7. Connect the blue cord, yellow cord, and red cord to the Cl electrode, Na electrode, and K
electrode, respectively.
8. Mount the three electrodes on the electrode block.
Attach the electrodes in order of Cl, Na, and K from the sample pot side.
9. Move the lock lever to clamp the electrodes.
10. Mount the tube connecting from the sample pot to the electrode block.
The two roller pumps are activated and solution flows into individual roller tubes.
times or so. Visually check that there are no air bubbles in the tube.
tube.
13. Select O K .
Refer to “ (3) Troubleshooting for Calibration Error – 1 of 2 (3264, 3265, 3266, 3267, 3268, 3269)”,
“ (4) Troubleshooting for Concurrent Reproducibility Error”, and “ (5) Troubleshooting for Daily
Variation” in section “3. TROUBLESHOOTING” in Part I.
II-46
Version 1.0
11.3 Manually Wash the K Electrode

If a calibration error such as slope 0 occurs frequently on the K electrode only, the REF solution may
have entered in the K electrode.
When these errors occur, manually wash the K electrode.
E (O-ring)
CI Cl electrode cord (blue)
Electrodes Na Na electrode cord (yellow)
F K K electrode cord (orange)
REF solution REF
(6) REF electrode block
Procedure for Wash the REF electrode

Materials:
Dry, clean cloth or paper towel

II-47
Version 1.0
5. Select C l e a n i n g K- E l e c t r o d e from the list of maintenance items.

7. Select O K .


2. Remove the K electrode from the electrode stack.
3. Remove the electrode electrical connection.
4. Remove the O-ring from the electrode.
5. Wash the O-ring and O-ring inserted groove on the electrode side using the nozzle-type wash
bottle filled with deionized water.
At this time there is no problem if deionized water comes in the electrode flow path during
washing.
6. Wipe the side face (location F in the tubing block diagram) of the REF electrode block in contact
with the K electrode using a clean cloth dampened with deionized water.
7. Sufficiently wipe the K electrode, O-ring, and REF electrode side faces that have been washed
with deionized water using a dry, clean cloth.
8. Connect the electrode wire to the electrode.
9. Attach the K electrode to the electrode stack.
Mount the electrodes in order of Cl, Na, and K from the sample pot side toward the lock lever
side.
Three O-rings are attached to one side of three electrodes. Be careful not to lose the
O-rings when mounting the electrodes.
CAUTION
10. Move the lock lever to clamp the electrodes.

11. Confirm that D ra i n - F l o w c e l l has been selected on the “ISE Maintenance: Maintenance
tab” window.
times or so. Verify that there are no air bubbles in the tube.
tube.
14. Select O K .
II-48
Version 1.0

17. Then, allows at least 5 minutes before performing calibration analysis.
CAUTION following values, the electrodes are stable.
• Difference between 1st and 2nd factors Na: 0.020, K: 0.045, Cl: 0.025
above, or if slope 0 is output at the first time, there may reside air inside the
electrode block. First, open the main cover and ISE cover. Next, from AU480
“Home” window select Menu List>Maintenance>User Maintenance>ISE
Maintenance>Maintenance to display the “ISE Maintenance: Maintenance
tab” window. Then select the check box of “ISE Maintenance”, select M I D /
R E F P r i m e and O K , and press the TA B L E R OTAT I O N /
D I A G button.
• If slope 0 is output in both calibrations, the electrodes may not be set correctly.
Repeat steps 1 to 15 in the above maintenance procedure.
If a calibration error occurs even after performing maintenance of the K electrode

manual-wash, contact Beckman Coulter Technical Services.
Refer to “ (3) Troubleshooting for Calibration Error – 1 of 2 (3264, 3265, 3266, 3267, 3268, 3269)” and
“ (4) Troubleshooting for Concurrent Reproducibility Error” in section “3. TROUBLESHOOTING” in Part
I.
11.4 Manually Wash and Replace the REF Electrode Block

Although auto-wash of the REF electrode is performed every day, there is a possibility that data will
be adversely affected by the accumulation of contaminants or crystals, reduction in flow rate, and
interference of noises.
If data is effected by these reasons, manually wash the REF electrode block or replace it.
CI
Na
REF electrode packing
K Cap screw
REF solution tube G Electrode unit
REF electrode cord (green)
REF
(6) REF electrode block
II-49
Version 1.0
Procedure for Replace the REF Electrode Block

Materials:
• REF electrode block : MU8245 (to replace)
• Wash solution : OSR0001

4. From AU480 “Home” window select Menu List>Maintenance>User Maintenance>ISE
5. Select C l e a n / Re p l a c e R E F e l e c t r o d e b l o c k from the list of maintenance

items.
7. Select O K .

TIP Each successive time this button is pressed, the MID solution is injected into the
tube.
II-50
Version 1.0

2. Gently lift up the block on which the REF electrode is mounted.
3. Pull the cord connecting to the REF electrode out of the electrode.
4. Loosen the cap screw on the REF electrode and gently remove the electrode along with the cap
screw.
Remove the REF electrode packing in the block.
5. While holding the REF electrode block by hand, pull the waste liquid drain tube out of the REF
electrode block.
6. Remove the REF solution tube (location G in the tubing block diagram) connected to the lower
side of the REF electrode block.
Dismount the REF electrode block.
7. Attach the REF solution tube (location G in the tubing block diagram) to a new REF electrode
block.
8. Insert the REF electrode packing into the block.
If the packing is cracked or broken. Replace the packing (part No.MU9900).
9. Insert the cap screw into the REF electrode insertion hole while turning it with fingers. Insert the
screw all the way until torque changes.
10. Verify that there are no air bubbles in the electrode block of the REF electrode.
If any air bubble is found, remove the air bubble by tapping the electrode block with fingers with
pointing it downward.
11. Insert the REF electrode into the REF electrode block.
At this time make sure that the REF electrode tip is correctly inserted into the packing.
The REF electrode tip can easily be inserted into the packing if it is damped with
deionized water.
TIP
12. After inserting the REF electrode tighten the cap screw by rotating it 1/4 to 1/2 turn, then secure
the REF electrode.
13. Connect the roller tube for waste liquid with the roller pump.
14. Reconnect REF electrode cord in place.
15. Remount the REF electrode block in place.
16. Move the lock lever to the clamp position.
17. Confirm that D ra i n - F l o w c e l l has been selected from the “Single Operation” buttons on
the “ISE Maintenance: Maintenance tab” window.
times or so. Verify that there are no air bubbles in the waste liquid tube on the REF electrode
block side.
tube.
II-51
Version 1.0
20. Select O K .
To prevent the REF electrode block from becoming deformed due to ultrasonic
cleaning, follow these precautions. If the REF electrode block has been deformed or
cracked, replace it.
CAUTION
• Do not perform ultrasonic cleaning for more than ten minutes.
• Use a cleaning liquid at room temperature.
• Use a sonicator rated at 600W or less.

If the output of the sonicator is uncertain, contact the manufacturer of the
sonicator.
Procedure for wash the REF electrode block:

Perform ultrasonic cleaning for the electrode block dismounted in step 6 of this
maintenance procedure for 10 minutes using a sonicator which is filled with a 2%
TIP
sample diluent of AU alkaline wash solution.
If no sonicator is available, soak it in the 2% sample diluent of AU alkaline wash
solution for more than 30 minutes. At this time make sure that the diluted wash
solution flows into the flow path in the electrode block.
After cleaning the electrode block, thoroughly rinse the wash solution from the block
under running water, wipe it with a clean cloth, and then implement the maintenance
procedure from step 7 to step 21.
Refer to section “10.1 Replace the REF Electrode and Packing” in section
“10. MAINTENANCE REQUIRED EVERY TWO YEARS OR 150,000 SAMPLES” in this chapter.
II-52
Version 1.0
11.5 Replace Reagents

The ISE reagent on board stability is specified below for each reagent. Replace expired reagent
bottles with new ones. When the quantity of reagent becomes insufficient, an alarm message will
appear. After the message displays the system is capable of analyzing approximately 180 samples.
Replace the reagent before the bottle empties.
On Board Stability of Reagents for the ISE Unit

The on board stability for the buffer solution, MID solution, and REF solution used for the ISE unit are
specified. If an expired reagent is used, data will be incorrect. Replace the corresponding reagent
before the on board stability expires.
On Board Stability: Check the package insert for on board stability claims.
On board stability Remark

Buffer solution 2 months after breaking seal New solution can be added only once.
MID solution 1 month after breaking seal No solution can be added.
REF solution 2 months after breaking seal New solution can be added only once.
Procedure for Replace ISE Reagents

Materials:
• Buffer solution tank : AUH1011
• MID solution tank : AUH1012
• REF solution tank : AUH1013
• Never replace reagents during analysis. A data error will result.
• Reference solution is highly concentrated. Do not contaminate other bottles with

CAUTION Reference solution.

2. Pull out the reagent bottle to be replaced.
3. Loosen the cap of the reagent bottle, then remove the aspiration tube.
• When the aspiration tube is removed from the tank, the solution in the tube may
drip in the system. If this occurs wipe it off with a dry cloth or paper towel wearing
protective gloves.
CAUTION
• Do not handle the aspiration tube with bare hands. Reagents will become
contaminated, resulting in defective data.
4. Place the aspiration tube in a new bottle, tighten the cap, and place it on the instrument.
Dispose of the old solution as liquid waste is handled.
II-53
Version 1.0
• Push the reagent tank all the way until it stops. If the reagent tank is positioned forward,
the liquid level sensor will not detect the reagent level. This may display an alarm message
indicating there is insufficient reagent.
CAUTION
• If the roller tube for the MID solution is removed from the roller pump, the solution in the
tubing flows backwards into each reagent tank. Do not remove the roller tube for the MID
solution from the roller pump when replacing either of the reagent tanks.
5. Push the reagent bottle to the back of its position.

7. Select one of following maintenance item from the list of maintenance items.
• To replace Buffer solution: Re p l a c e B u f f e r L i q u i d
• To replace MID solution: Re p l a c e M I D L i q u i d
• To replace REF solution: Re p l a c e R E F L i q u i d

8. Select one of following buttons from the “Single Operation” buttons.
• To replace Buffer solution: B u f f e r P r i m e
• To replace MID solution: M I D / R E F P r i m e
• To replace REF solution: M I D / R E F P r i m e

9. Select O K .
10. Press the TA B L E R OTAT I O N / D I A G button. The reagent is primed for approximately
90 seconds.
12. Select O K .
II-54
Version 1.0
11.6 Replace the Buffer Syringe

Lock nut
Mounting groove
Fixing screws
Syringe case
Mounting groove
Piston fixing screw
Procedure for replace the buffer syringe

Materials:
R Syringe: ZM0112

3. Loosen the piston fixing screw located at the bottom of the syringe.
4. Loosen the lock nut located at the top of the syringe.
5. Pull the syringe case forward to remove the syringe from the mounting grooves.
When removing the fixing screws from the syringe case do not use excessive force,
or the syringe case may be damaged.
CAUTION
6. While holding the case head and syringe case, turn the syringe case counterclockwise to remove
it.
II-55
Version 1.0
7. Pull out the syringe from the case head.

Case head
O-ring
Syringe
ZM0112
Syringe case
If the O-ring remains in the case head, carefully remove the O-ring with tweezers. Be careful not
to scratch the case head.
• Set the R syringe in place. The S syringe and R syringe are identified with the piston shaft
diameter.
Piston shaft diameter: D
CAUTION
D = 5mm: R syringe
D = 2mm: S syringe
• Do not pull out the piston from a new syringe. Once the piston has been pulled out, the
syringe cannot maintain the dispensing accuracy even if the piston is inserted in the
original position.
8. Insert the new R syringe into the case head.

9. Mount the syringe case on the case head.
10. Insert the syringe into the mounting grooves by holding the case head. Tighten the lock nut while
holding the case head by hand.
Mounting groove
Lock nut
Fixing screws
Case head
Syringe case
Piston fixing screw
11. Tighten the piston clamp screw.
II-56
Version 1.0
13. Select Re p l a c e I S E r e a g e n t ( b u f f e r ) s y r i n g e from the list of maintenance

items.
14. Select B u f f e r P r i m e from the “Single Operation” buttons.
15. Select O K .
18. Select O K .
If air bubbles get in the syringe, the bubbles will affect the dispense amount of buffer solution
and cause an error in data. If air bubbles have gotten in the syringe, perform B u f f e r
P r i m e until the air bubbles are fully eliminated.
CAUTION If air bubbles are not eliminated after the B u f f e r P r i m e is repeatedly performed, re-
exercise steps 8 to 16 in the above procedure.

Refer to “ (1) Troubleshooting for ISE Sample Pot Liquid level Sensor Error -1 (3186, 3187)” in
section “3. TROUBLESHOOTING” in Part I.
Avoid getting AU Alkali wash solutions on the syringe case, be sure to wipe any excess off the
syringe case with a damp cloth.
CAUTION
II-57
Version 1.0
11.7 Procedure for Enhanced Cleaning of the Electrode Line

(Manual)
To help eliminate sample buildup and residue from high volume sample testing, this ISE cleaning may
be necessary on an as-needed basis. This method should be used when the ISE calibration slopes
are affected, or if a build-up/residue is present upon inspection of the sample pot or T-tubing. This is
the ONLY ISE cleaning procedure recommended by Beckman Coulter.
Materials:
• Pipette
solution.
Clean the Electrodes

II-58
Version 1.0
5. Select M a n u a l - w a s h i n g E l e c t r o d e L i n e from the list of maintenance items.

6. Collect 1 mL of the wash solution with a pipette and dispense it gently in the sample pot so that
no wash solution is splashed. The total amount of wash solution to be used in this procedure
including the following operations is approximately 5 mL.
Pipette
Mix bar Sample pot

The window to select the D ra i n - F l o w c e l l operation will be displayed.
8. Select O K .
The wash solution in the sample pot is drained through the electrodes.
10. After completing the operation in step 9, press the TA B L E R OTAT I O N / D I A G button
again.
The system will start with the Prime operation with the MID solution.

12. Select C a n c e l .
13. Repeat the procedure in steps 6 to 10.
II-59
Version 1.0
Clean the Bypass Tube

1. Re-collect 1 mL of the wash solution with a pipette and dispense it gently in the sample pot so
that the wash solution is not splashed.
2. Select D ra i n B y p a s s .
The Drain Bypass operation window is displayed.
3. Select O K .
The wash solution in the sample pot is drained through the bypass tube (without passing through
the electrodes).
5. Re-collect 1 mL of the wash solution with a pipette and dispense it gently in the sample pot so
that the wash solution is not splashed.
The wash solution in the sample pot is drained through the bypass tube (without passing through
the electrodes).
7. Select To t a l P r i m e from the “Single Operation” buttons.
The window to select the Total Prime will be displayed.
8. Select O K .
10. After completing the Prime resolution, re-press the TA B L E R OTAT I O N / D I A G button.
12. Select O K .
13. Perform auto-wash of the sample pot and electrode line to be implemented in daily maintenance.
Refer to section “5.2 Auto-wash the Sample Pot and Electrode Line” in section
“5. DAILY MAINTENANCE” in this chapter.
14. Perform calibration of the ISE unit.
II-60
Version 1.0
11.8 Replace the Buffer Syringe Case and Case Head

Lock nut
Mounting groove
Fixing screws
Syringe case
Mounting groove
Piston fixing screw
Procedure for replace the syringe case and the case head
Materials:
ISE Syringe Case: ZM1362

3. Loosen the fixing screws of the two tubes connected to the case head and remove the tubes.
Tubes
Case head
Fixing screws
Syringe case
4. Loosen the piston fixing screw located at the bottom of the syringe.
5. Loosen the lock nut located at the top of the syringe.
6. Pull the syringe case to the front to remove the syringe from the mounting grooves.
To avoid damaging the syringe case, do not apply excessive force to the fixing
screws when removing them.
CAUTION
7. While holding the case head and syringe case, turn the syringe case counterclockwise to remove
it.
II-61
Version 1.0
8. Pull out the syringe from the case head.
Case head
O-ring
Syringe
ZM0112
Syringe case
If the O-ring remains in the case head, carefully take out the O-ring with tweezers be careful not
to scratch the case head.
• Set the syringe case and the case head for ISE.
• The syringe case and the case head are identified with their figures and color.
CAUTION
F
R
O
N
T
ISE syringe case head S-syringe case head

(Gray) R-syringe case head
(Clear)
Syringe case for ISE S-syringe case

(Separate type) R-syringe case
(Whole type)
9. Insert the new R syringe for ISE into the new case head.
Avoid getting AU Alkali wash solutions on the syringe case and syringe head. The
strong alkali adhering to the syringe case and case head will cause cracks on them.
If the strong alkali has adhered to, remove the syringe case and case head and
CAUTION wash them with water.
10. Mount the syringe case on the case head.
II-62
Version 1.0
11. Insert the syringe into the mounting grooves by holding the case head. Tighten the lock nut while
holding the case head by hand.
Mounting groove
Lock nut
Fixing screws
Case head
Syringe case
Piston fixing screw
12. Tighten the piston clamp screw.

13. Fix the removed two tubes to the initial positions of the case head.
15. Select Re p l a c e B u f f e r S y r i n g e C a s e from the list of maintenance items.

16. Select B u f f e r P r i m e from the “Single Operation” buttons.
17. Select O K .
20. Select O K .
If air bubbles get in the syringe, it will affect the dispense amount of buffer solution
and cause a data error. If air bubbles have gotten in the syringe, perform B u f f e r
P r i m e until the air bubbles are fully eliminated.
CAUTION If air bubbles are not eliminated after the B u f f e r S o l u t i o n P r i m e is
repeatedly performed, repeat step 9 to 18 in the above procedure.
II-63
Version 1.0
12 RESETTING THE SYSTEM WHEN SWITCHED

TO THE STOP MODE WHILE PERFORMING
MAINTENANCE
After performing maintenance in Stop mode, reset the system by performing the following:
1. Determine and correct the cause of the error.
2. Confirm that “Analyzer Maintenance: Maintenance tab” window is displayed from the AU480
“Home” window in following order.
4. Select C a n c e l .
5. Select the S t o p / S t a n d b y button. After initialization the analyzer goes into Warm up or
Standby mode.
II-64
Version 1.0

Beckman Coulter Chemistry Analyzer: Ise User Guide

Uploaded by

Document Information

Original Title

Copyright

Available Formats

Share this document

Share or Embed Document

Sharing Options

Did you find this document useful?

Is this content inappropriate?

Copyright:

Available Formats

Beckman Coulter Chemistry Analyzer: Ise User Guide

Uploaded by

Copyright:

Available Formats

BECKMAN COULTER

Beckman Coulter Biomedical Limited.

Beckman Coulter Biomedical Limited. is the EC authorized representative of:

Beckman Coulter Biomedical K.K.

Beckman Coulter Mishima K.K.

Lumi-Phos ® 530 is a registered trademark of Lumigen, Inc., Southfield, Michigan, USA.

Manufacturer Authorized Representative in the European

Document Change Notification

Document Software Date ISE User Guide change description

Precautions in Using This Manual

Aim of this manual

How AU480 Documents are Organized

How This Manual is Structured

Part I: ISE TROUBLESHOOTING

Part II: ISE MAINTENANCE FLOW

Document Change Notification ...................................................................................Behind Cover Page

Part I ISE TROUBLESHOOTING I-1

Part II ISE MAINTENANCE FLOW II-1

1 ISE WORKFLOWS AND PROBLEMS THAT CAN

(1) ISE sample pot liquid level

(3) Calibration error

Notifies that the liquid level

(5) Daily variation Refer to page I-9.

(1) ISE sample pot liquid level

(6) ISE data low-value

(1) ISE sample pot liquid level

If another error occurs: Refer to page I-3.

2 OTHER ERRORS IN ISE

These Errors indicate abnormal communication conditions

Does liquid remain Does the liquid level sensor and

Properly install the mixing unit.

Is any tubing detached?

Reattach the tubing in place.

Wash the tubing.

Perform user maintenance prime operation.

Does aspiration operate properly?

CALL Contact Beckman Coulter Technical Services.

(2) a Troubleshooting for ISE Sample Pot Overflow Sensor Error

Has the buffer solution been

Is any tubing detached?

Reattach the tubing in place.

Check the liquid level of the buffer solution.

Check the ISE buffer syringe.

Has the problem been corrected?

Is the level sensor connector detached?

Remove the liquid level sensor connector and reconnect it.

Connect the level sensor connector.

Has the problem been corrected?

(3) Troubleshooting for Calibration Error – 1 of 2 (3264, 3265,

Is the probe factor 0 for all items?

Continue “Troubleshooting for

Has the electrode set properly?

Has the reagent/standard solution set properly?

Re-set the reagent/standard solution.

Has the buffer solution been dispensed properly?

Check the ISE buffer syringe.

Has the problem been corrected?

Has the problem been corrected?

CALL Contact Beckman Coulter Technical Services .

The tubing gets dirty.

The indicated electrode/REF electrode has deteriorated.

Has the problem been corrected?

(3) (Continued) Troubleshooting for Calibration Error – 2 of 2